##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513179_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2015650 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.893582219135265 31.0 31.0 34.0 28.0 34.0 2 31.005844268598217 33.0 31.0 34.0 27.0 34.0 3 31.110183811673654 33.0 31.0 34.0 27.0 34.0 4 34.50258824696748 37.0 35.0 37.0 31.0 37.0 5 34.266993773720635 37.0 35.0 37.0 30.0 37.0 6 34.18298464515169 37.0 35.0 37.0 30.0 37.0 7 34.057066951107586 37.0 35.0 37.0 30.0 37.0 8 34.033524669461464 37.0 35.0 37.0 30.0 37.0 9 35.60112817205368 39.0 37.0 39.0 30.0 39.0 10 35.45028799642795 39.0 35.0 39.0 30.0 39.0 11 35.45898643117605 39.0 35.0 39.0 30.0 39.0 12 35.399286582492 39.0 35.0 39.0 30.0 39.0 13 35.365459777243075 39.0 35.0 39.0 30.0 39.0 14 36.53937637982785 40.0 37.0 41.0 29.0 41.0 15 36.51793614962915 40.0 37.0 41.0 29.0 41.0 16 36.47930345049984 40.0 37.0 41.0 29.0 41.0 17 36.4134993674497 40.0 37.0 41.0 29.0 41.0 18 36.36657058517104 40.0 37.0 41.0 29.0 41.0 19 36.44728598715054 40.0 37.0 41.0 29.0 41.0 20 36.413388733162996 40.0 37.0 41.0 29.0 41.0 21 36.36105077766477 40.0 37.0 41.0 29.0 41.0 22 36.303589412844495 40.0 37.0 41.0 29.0 41.0 23 36.2211564507727 40.0 37.0 41.0 28.0 41.0 24 36.15994939597648 40.0 37.0 41.0 28.0 41.0 25 36.0989963535336 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48415616345931056 1101 2 0.5410244922495444 1101 3 0.46207414080156894 1101 4 0.562349206533014 1101 5 0.4620988738354157 1101 6 0.47528362983281625 1101 7 0.5333795750238153 1101 8 0.5550630961938907 1101 9 0.5210623676969917 1101 10 0.6305209270551799 1101 11 0.6799278159158959 1101 12 0.6809161143141864 1101 13 0.6214131497973128 1101 14 0.6913934355909319 1101 15 0.812438124669498 1101 16 0.6338709679091039 1101 17 0.7527533270421003 1101 18 0.7134527991747319 1101 19 0.814688611214585 1101 20 0.7941112402931907 1101 21 0.7175531818983174 1101 22 0.7722937394165967 1101 23 0.7919466396451824 1101 24 0.7624105441782092 1101 25 0.7877860707848825 1102 1 0.5011333738566748 1102 2 0.5708481895544892 1102 3 0.45579347361112355 1102 4 0.5729347463088033 1102 5 0.6208773176337843 1102 6 0.625237633201472 1102 7 0.5060365052937144 1102 8 0.5687221680988586 1102 9 0.6051192174063189 1102 10 0.6795575236235081 1102 11 0.6619695213008256 1102 12 0.6717742838343739 1102 13 0.7292054577248166 1102 14 0.7888268634433544 1102 15 0.6502986697960438 1102 16 0.587668282290764 1102 17 0.8913347962489482 1102 18 0.7791242901701594 1102 19 0.8370235650650741 1102 20 0.8872175284049248 1102 21 0.7840020292573016 1102 22 0.85685508975007 1102 23 0.9140185087169712 1102 24 0.7758519297137312 1102 25 0.781110244673414 1103 1 0.5388157764405825 1103 2 0.539717158818366 1103 3 0.5156790365751398 1103 4 0.6516025654837705 1103 5 0.6264775402174436 1103 6 0.4647655886759381 1103 7 0.5130908898289945 1103 8 0.587236752057656 1103 9 0.6251455887317405 1103 10 0.5750701093880508 1103 11 0.5517435394968189 1103 12 0.6225290777960168 1103 13 0.715242731690779 1103 14 0.7026550935183238 1103 15 0.7060542280642466 1103 16 0.7051714522384458 1103 17 0.8435959889410825 1103 18 0.7195200360068839 1103 19 0.7118035382538608 1103 20 0.7909604310762575 1103 21 0.6882601515265563 1103 22 0.6444886097061726 1103 23 0.6536917374227826 1103 24 0.7543499518643273 1103 25 0.684648159227244 1104 1 0.33200922327084115 1104 2 0.4924654990481514 1104 3 0.4347344492475713 1104 4 0.602904553945578 1104 5 0.5791535628588349 1104 6 0.5798190656294651 1104 7 0.589745902307854 1104 8 0.6450565670768782 1104 9 0.6600961898377093 1104 10 0.8253466018218916 1104 11 0.8221101842325922 1104 12 0.716819794501383 1104 13 0.727239838987245 1104 14 0.8048012402958378 1104 15 0.847471000307344 1104 16 0.8105436159480703 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6 6756.0 7 5401.0 8 4355.0 9 3873.0 10 3450.0 11 3181.0 12 2899.0 13 2800.0 14 2855.0 15 2855.0 16 2887.0 17 2971.0 18 3345.0 19 4109.0 20 5037.0 21 6770.0 22 8530.0 23 10215.0 24 11889.0 25 13646.0 26 14949.0 27 16568.0 28 18839.0 29 22654.0 30 28604.0 31 36400.0 32 47162.0 33 61122.0 34 81465.0 35 109920.0 36 157730.0 37 256704.0 38 1007414.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.89319430535296 19.20425035970508 13.445241580167638 27.457313754774326 2 14.049960332010405 20.668039706690074 39.245558996149306 26.03644096515022 3 16.907784246940654 24.856949361090923 29.99203689324724 28.24322949872119 4 11.390519774201904 17.219238283672503 37.585966701825654 33.804275240299944 5 12.880782174275277 38.015608134342365 35.969212711722186 13.134396979660174 6 31.94568514403589 37.412206161556085 17.879013334859497 12.76309535954852 7 27.701971052064795 31.514104252664676 22.60040117792668 18.18352351734385 8 25.36640841848709 36.31281340336831 20.507111831352397 17.81366634679221 9 26.35956214584852 14.728633325676228 19.63556475951809 39.27623976895717 10 14.354783174719413 28.896956021722715 34.890349506297426 21.85791129726045 11 34.5425545109518 22.344305807059758 24.236747451194404 18.876392230794035 12 22.754942574355667 24.818892168779303 31.64011609158336 20.78604916528167 13 28.762682013246348 21.3816882891375 25.802594696499888 24.053035001116267 14 21.197968062321486 21.23969234173425 27.53866937750451 30.02367021843975 15 23.50442785205765 29.694143328454842 23.242527224468535 23.558901595018977 16 22.927045866097785 27.676332696648725 26.59246396943914 22.804157467814353 17 22.211360732406245 27.99776705412413 27.256032055178576 22.534840158291054 18 22.28572647943911 27.284862993576457 28.34144216034238 22.087968366642052 19 23.463597350730534 26.977203383523925 27.202044005655747 22.357155260089797 20 23.729070027038425 26.85605139781212 27.348448391337783 22.066430183811676 21 23.42344264263311 26.508042662676505 26.909506029392354 23.159008665298025 22 23.30600382606487 27.199838463858768 26.78865555890711 22.705502151169252 23 22.785096510690995 26.936217209584782 27.032172214923335 23.246514064800884 24 23.130652643067993 26.757224716592663 27.40039193312331 22.711730707216034 25 23.053231516614012 26.76241089714077 27.142288732953375 23.042068853291845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 172.0 1 172.0 2 277.5 3 383.0 4 383.0 5 383.0 6 870.0 7 1357.0 8 1357.0 9 1357.0 10 1979.0 11 2601.0 12 2601.0 13 2601.0 14 4984.0 15 7367.0 16 7367.0 17 7367.0 18 14143.5 19 20920.0 20 20920.0 21 20920.0 22 33607.5 23 46295.0 24 46295.0 25 46295.0 26 66552.0 27 86809.0 28 86809.0 29 86809.0 30 111737.0 31 136665.0 32 136665.0 33 136665.0 34 157843.0 35 179021.0 36 179021.0 37 179021.0 38 199348.0 39 219675.0 40 219675.0 41 219675.0 42 233925.0 43 248175.0 44 248175.0 45 248175.0 46 258862.0 47 269549.0 48 269549.0 49 269549.0 50 263132.5 51 256716.0 52 256716.0 53 256716.0 54 230796.0 55 204876.0 56 204876.0 57 204876.0 58 176884.5 59 148893.0 60 148893.0 61 148893.0 62 122336.0 63 95779.0 64 95779.0 65 95779.0 66 73796.5 67 51814.0 68 51814.0 69 51814.0 70 37727.0 71 23640.0 72 23640.0 73 23640.0 74 16563.5 75 9487.0 76 9487.0 77 9487.0 78 6583.5 79 3680.0 80 3680.0 81 3680.0 82 2461.5 83 1243.0 84 1243.0 85 1243.0 86 838.0 87 433.0 88 433.0 89 433.0 90 262.0 91 91.0 92 91.0 93 91.0 94 50.0 95 9.0 96 9.0 97 9.0 98 4.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17785825912236747 2 0.008483615707092005 3 0.005209237714881056 4 0.002182918661473966 5 0.07798973035993352 6 0.3103217324436286 7 0.028973284052290823 8 0.01369285342197306 9 0.004613896261751792 10 0.0031751544166894055 11 0.0 12 0.0 13 0.0 14 0.0018356361471485625 15 0.0 16 0.0 17 0.018604420410289486 18 0.02788182472155384 19 0.0 20 0.0 21 0.0011906829062585272 22 9.922357552154392E-5 23 0.006052638106814179 24 0.0 25 9.922357552154392E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2015650.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.08892232598623 #Duplication Level Percentage of deduplicated Percentage of total 1 82.20070399462975 59.25760165410249 2 12.08113030219922 17.418313279307164 3 3.0590998304959123 6.615816302041725 4 1.1409094395824377 3.289877278841714 5 0.491333134705679 1.7709838091990515 6 0.2755697367692178 1.1919315209609167 7 0.17129749660786392 0.8644056349320134 8 0.10896836825681076 0.6284329788203755 9 0.08007198569778812 0.5195072841709795 >10 0.3588681357935075 4.413812612740306 >50 0.019040118855747055 0.9516785610661367 >100 0.01094424013870489 1.4981721450080367 >500 0.0013754775115889187 0.6655892916539669 >1k 6.877387557944215E-4 0.9138776471550863 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2855 0.14164165405700394 No Hit GTACATGGGAAGCAGTGGTATCAAC 2358 0.11698459553990029 No Hit CCCATGTACTCTGCGTTGATACCAC 2302 0.11420633542529705 No Hit TATCAACGCAGAGTACTTTTTTTTT 2054 0.1019026120606256 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 4.961178776077196E-5 13 4.961178776077196E-5 0.0 0.0 0.0 4.961178776077196E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCCG 35 0.0021871745 16.267065 9 ACCGTCC 70 1.1026875E-4 12.200603 8 GCGCCAC 105 2.7486567E-7 11.7528105 13 TAGGACC 460 0.0 11.586755 4 TACACCG 60 0.005795852 11.103973 5 CCCTATA 130 3.1721356E-8 10.983591 2 GGCGAGG 535 0.0 10.819373 19 TGACGAA 115 8.9506284E-7 10.726833 10 CGTGCGC 80 3.811859E-4 10.675262 10 GTCTAAG 190 9.094947E-12 10.52584 1 GACGAAA 145 1.465196E-8 10.472834 11 TAGGACT 265 0.0 10.415586 4 ACCGTGC 120 1.546141E-6 10.280137 8 AGGACGT 885 0.0 10.216731 5 AGGACTG 375 0.0 10.1522045 5 TGCACCG 75 0.0026075915 10.152204 5 TGGCGAG 1040 0.0 10.131331 18 ACACCGC 85 6.487345E-4 10.076803 6 CTAACAC 190 9.640644E-11 10.019121 3 TTAGGAC 790 0.0 10.000097 3 >>END_MODULE