FastQCFastQC Report
Thu 26 May 2016
SRR1513178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069686
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT24620.11895524248605827No Hit
GTCCTAAAGTGTGTATTTCTCATTT23280.11248083042548482No Hit
GTCCTACAGTGTGCATTTCTCATTT20870.10083655201803558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7100.013.2798974
TATACCG657.947527E-411.7059035
CGTATCC658.085863E-411.681849517
GTCCTAG2550.011.6026411
AATGTCC5200.011.3140558
GTATTAC1451.1277734E-911.1896451
TGTAGGA18750.011.1815692
GTAGGAC18200.011.145643
AGGACCT12250.011.1026915
AAACCCG600.005826154711.0962215
GTCCTAC14900.011.0173611
AGGACGT13300.010.7982565
AATCCCG803.8031253E-410.6779419
GTCTAGG1803.274181E-1110.6045661
GGACGTG13400.010.5511786
CTGTAGG19100.010.5434921
TGGCGAG14900.010.51046918
ACGAAAT1106.1173832E-610.35361612
TCCTACA17000.010.31454852
GGCGAGA9150.010.26949519
Produced by FastQC (version 0.11.5)