FastQCFastQC Report
Thu 26 May 2016
SRR1513178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069686
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30160.14572258787081713No Hit
GTCCTACAGTGGACATTTCTAAATT24720.11943840756520555No Hit
CTTTAGGACGTGAAATATGGCGAGG23980.11586298597951573No Hit
GTCCTAAAGTGTGTATTTCTCATTT21660.10465355614329903No Hit
GTACATGGGAAGCAGTGGTATCAAC21380.10330069392168667No Hit
CTGTAGGACGTGGAATATGGCAAGA20890.10093318503386504No Hit
TATCAACGCAGAGTACTTTTTTTTT20700.10001517138348523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG604.013379E-412.6962111
TAGGACC7250.012.2104854
GTAGGAC16450.011.5740043
TGTAGGA17050.011.3344852
GGCGAGG8000.011.03119
GGACGTG14400.010.9718786
TGGCGAG15450.010.93551818
AGGACGT14650.010.8509265
GTCCTAC16600.010.8414931
ATATGGC25850.010.46508215
AGGACCT12100.010.4629545
GACGTGA8850.010.402677
TAGGACG15700.010.3677494
TCCTACA18050.010.2846032
GGACCTG12000.010.23160656
TTTATAC3100.010.1337883
CGGGACT856.4753165E-410.0788644
TATGGCG16300.010.07434516
TTAGACA2850.010.0199244
TGGAATA20400.010.00287111
Produced by FastQC (version 0.11.5)