##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513178_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2069686 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.67788157237378 31.0 31.0 34.0 27.0 34.0 2 30.799935835677488 33.0 31.0 34.0 27.0 34.0 3 30.88979439393222 33.0 31.0 34.0 27.0 34.0 4 34.27416815884148 37.0 35.0 37.0 30.0 37.0 5 34.03543725956498 37.0 35.0 37.0 30.0 37.0 6 33.95641947619107 37.0 35.0 37.0 30.0 37.0 7 33.81999201811289 37.0 35.0 37.0 29.0 37.0 8 33.7832690562723 37.0 35.0 37.0 29.0 37.0 9 35.33119130148245 39.0 35.0 39.0 29.0 39.0 10 35.163005885916995 39.0 35.0 39.0 28.0 39.0 11 35.178092715513365 39.0 35.0 39.0 28.0 39.0 12 35.11341140636792 39.0 35.0 39.0 28.0 39.0 13 35.080969770293656 39.0 35.0 39.0 28.0 39.0 14 36.232560398050715 40.0 36.0 41.0 27.0 41.0 15 36.20403771393342 40.0 36.0 41.0 27.0 41.0 16 36.16587588648713 40.0 36.0 41.0 27.0 41.0 17 36.09928124362826 40.0 36.0 41.0 27.0 41.0 18 36.0512111499039 40.0 36.0 41.0 27.0 41.0 19 36.12604424052731 40.0 37.0 41.0 27.0 41.0 20 36.090197256975216 40.0 37.0 41.0 27.0 41.0 21 36.03232519329019 40.0 37.0 41.0 27.0 41.0 22 35.97659306774071 40.0 36.0 41.0 27.0 41.0 23 35.89279195008325 40.0 36.0 41.0 27.0 41.0 24 35.83508174669974 40.0 36.0 41.0 27.0 41.0 25 35.77652793708804 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5229293115131668 1101 2 0.49777654236099167 1101 3 0.43263807856954983 1101 4 0.6624314024520501 1101 5 0.46288939701735643 1101 6 0.4422968606686055 1101 7 0.4748072086423676 1101 8 0.6022429004663081 1101 9 0.5686720577952329 1101 10 0.6626977948604065 1101 11 0.6408011574510937 1101 12 0.5444197890791571 1101 13 0.6866710976950188 1101 14 0.7017089225229256 1101 15 0.7649626690887175 1101 16 0.7759923852905928 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0.5935047808761382 1103 5 0.5284193996383877 1103 6 0.4592209893028212 1103 7 0.4676412708705513 1103 8 0.5597348024967488 1103 9 0.6448910134625692 1103 10 0.5689162398640448 1103 11 0.6829800042934551 1103 12 0.6180504415409089 1103 13 0.6510871481090916 1103 14 0.7531104686771855 1103 15 0.741995216423156 1103 16 0.7582486555214132 1103 17 0.7582504070213787 1103 18 0.7236009418246852 1103 19 0.6906235293063077 1103 20 0.7258704822229163 1103 21 0.6696598903503599 1103 22 0.7392747926369978 1103 23 0.8208271554013322 1103 24 0.8863521964170644 1103 25 0.8137799377501835 1104 1 0.4993043955982728 1104 2 0.5790555724214848 1104 3 0.5054186601358133 1104 4 0.6682145730971243 1104 5 0.6768287369678632 1104 6 0.5819629300312528 1104 7 0.6550642549454224 1104 8 0.6407844279428687 1104 9 0.6706839901708221 1104 10 0.8395052105668768 1104 11 0.8591694553402007 1104 12 0.8585223312187864 1104 13 0.761767231664713 1104 14 0.9870123883709496 1104 15 0.8356406948104791 1104 16 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-1.429841411642986 2216 4 -1.192927428733853 2216 5 -1.5744490155356914 2216 6 -1.7045288389960263 2216 7 -2.0021105359805667 2216 8 -2.006190808932793 2216 9 -2.1648734749928735 2216 10 -2.14103784828837 2216 11 -2.2141061404294575 2216 12 -2.395173322176106 2216 13 -2.2964631794065227 2216 14 -2.5490075951165565 2216 15 -2.5125342252948855 2216 16 -2.4931416035079863 2216 17 -2.7385424235709834 2216 18 -2.581410373107836 2216 19 -2.5744379570498737 2216 20 -2.5602533590544567 2216 21 -2.836841166218754 2216 22 -2.9218780123701507 2216 23 -2.8286801490085054 2216 24 -2.741988344895965 2216 25 -2.9122911652580186 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51825.0 3 0.0 4 864.0 5 6720.0 6 7886.0 7 6152.0 8 4866.0 9 4375.0 10 3978.0 11 3584.0 12 3363.0 13 3207.0 14 3073.0 15 3031.0 16 3029.0 17 3373.0 18 3630.0 19 4425.0 20 5512.0 21 7180.0 22 9067.0 23 10971.0 24 12484.0 25 14220.0 26 15662.0 27 17356.0 28 19779.0 29 23430.0 30 29812.0 31 38387.0 32 49158.0 33 63715.0 34 84607.0 35 113771.0 36 163388.0 37 270038.0 38 1003768.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.2027682826599 19.521204042258034 13.648913297069567 27.6271143780125 2 13.876453059246305 20.600788687966688 39.422994685223046 26.09976356756396 3 16.912084105019176 24.789102064502224 29.962799628721065 28.336014201757536 4 11.445783423598062 17.429346851863574 37.298136450892976 33.82673327364539 5 12.861501416704543 38.022802657408924 35.879855718554474 13.23584020733205 6 32.06026366078573 37.36304319273719 17.704849386890047 12.871843759587037 7 27.71613288618434 31.431016925642872 22.521433138611837 18.331417049560947 8 25.2425903688688 36.58394682606605 20.339319669378355 17.834143135686794 9 26.283474512187638 14.662033236422356 19.71837995513136 39.33611229625865 10 14.527649775151852 28.824918982672653 34.742868131767246 21.904563110408244 11 34.3910622190999 22.141571233510785 24.56329124321274 18.904075304176576 12 23.06572108039577 24.6622434514221 31.561840781645138 20.710194686536994 13 28.873993446348866 21.571967921704065 25.786520274089884 23.76751835785718 14 21.12427324914225 21.529461161922 27.340085820983862 30.006179767951885 15 23.263335597767004 30.39002051518926 23.064948016269135 23.281695870774602 16 22.61516964409094 27.681058865934254 27.047629447172177 22.656142042802628 17 22.05091725185439 28.29439626072656 27.377603503442504 22.277082983976545 18 22.06283492758218 27.25397080088773 28.79860074699 21.88459352454009 19 23.684897129322998 26.800200610140863 27.13585539062447 22.37904686991167 20 23.88014413780641 26.886493893276565 27.39033843781134 21.843023531105686 21 23.275817084413212 26.449164023740913 26.879086388400893 23.395932503444982 22 23.488646823067278 27.41876179225341 26.690245133921348 22.402346250757965 23 22.58773868938815 27.207413280839667 27.190839893833683 23.014008135938496 24 23.236568252382245 26.71791759716208 27.523981898703475 22.5215322517522 25 22.950363437123737 26.94710883400558 27.28851360884077 22.814014120029917 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 148.0 1 148.0 2 238.0 3 328.0 4 328.0 5 328.0 6 838.0 7 1348.0 8 1348.0 9 1348.0 10 2041.0 11 2734.0 12 2734.0 13 2734.0 14 5120.5 15 7507.0 16 7507.0 17 7507.0 18 14575.5 19 21644.0 20 21644.0 21 21644.0 22 35062.5 23 48481.0 24 48481.0 25 48481.0 26 69622.0 27 90763.0 28 90763.0 29 90763.0 30 117393.5 31 144024.0 32 144024.0 33 144024.0 34 166382.5 35 188741.0 36 188741.0 37 188741.0 38 207163.5 39 225586.0 40 225586.0 41 225586.0 42 239549.0 43 253512.0 44 253512.0 45 253512.0 46 264145.0 47 274778.0 48 274778.0 49 274778.0 50 267581.5 51 260385.0 52 260385.0 53 260385.0 54 234088.5 55 207792.0 56 207792.0 57 207792.0 58 179373.5 59 150955.0 60 150955.0 61 150955.0 62 123906.0 63 96857.0 64 96857.0 65 96857.0 66 75054.5 67 53252.0 68 53252.0 69 53252.0 70 39001.5 71 24751.0 72 24751.0 73 24751.0 74 17464.0 75 10177.0 76 10177.0 77 10177.0 78 7092.0 79 4007.0 80 4007.0 81 4007.0 82 2689.5 83 1372.0 84 1372.0 85 1372.0 86 907.5 87 443.0 88 443.0 89 443.0 90 264.5 91 86.0 92 86.0 93 86.0 94 50.0 95 14.0 96 14.0 97 14.0 98 7.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17935087737946723 2 0.0083587558692478 3 0.004010270156922354 4 0.0015944447611859964 5 0.0727646609195791 6 0.30695477478226163 7 0.027878625066797575 8 0.012127443486596516 9 0.004300169204410717 10 0.00323720603028672 11 0.0 12 0.0 13 0.0 14 0.0014011787295270877 15 0.0 16 0.0 17 0.019664818721293956 18 0.028120207606371204 19 0.0 20 0.0 21 4.831650791472716E-4 22 4.8316507914727166E-5 23 0.004976600315216897 24 0.0 25 9.663301582945433E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2069686.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.14871824475793 #Duplication Level Percentage of deduplicated Percentage of total 1 83.15556771166406 59.995676253117416 2 11.448799918249504 16.52032479084782 3 2.8580432072709776 6.186124622782142 4 1.0329419566131801 2.9810175276349318 5 0.4809456070301184 1.734980454633504 6 0.2795413341597199 1.2101129373632014 7 0.16248647316457232 0.8206233539654604 8 0.10632566446713632 0.613700832626087 9 0.07437086218484162 0.48291861432544736 >10 0.3639440693105359 4.500247845303764 >50 0.020865315796749204 1.0131828111821997 >100 0.013486512738811502 1.8317775503639828 >500 0.0018769571447668216 0.9059601491863004 >1k 8.044102048999303E-4 1.2033522566678077 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3016 0.14572258787081713 No Hit GTCCTACAGTGGACATTTCTAAATT 2472 0.11943840756520555 No Hit CTTTAGGACGTGAAATATGGCGAGG 2398 0.11586298597951573 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2166 0.10465355614329903 No Hit GTACATGGGAAGCAGTGGTATCAAC 2138 0.10330069392168667 No Hit CTGTAGGACGTGGAATATGGCAAGA 2089 0.10093318503386504 No Hit TATCAACGCAGAGTACTTTTTTTTT 2070 0.10001517138348523 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.831650791472716E-5 0.0 0.0 0.0 0.0 2 4.831650791472716E-5 0.0 0.0 0.0 0.0 3 4.831650791472716E-5 0.0 0.0 0.0 0.0 4 4.831650791472716E-5 0.0 0.0 0.0 0.0 5 4.831650791472716E-5 0.0 0.0 0.0 0.0 6 9.663301582945432E-5 0.0 0.0 0.0 0.0 7 9.663301582945432E-5 0.0 0.0 0.0 0.0 8 9.663301582945432E-5 0.0 0.0 0.0 0.0 9 9.663301582945432E-5 0.0 0.0 0.0 4.831650791472716E-5 10 9.663301582945432E-5 0.0 0.0 0.0 4.831650791472716E-5 11 9.663301582945432E-5 0.0 0.0 9.663301582945432E-5 4.831650791472716E-5 12 9.663301582945432E-5 0.0 0.0 9.663301582945432E-5 2.415825395736358E-4 13 9.663301582945432E-5 0.0 0.0 9.663301582945432E-5 3.382155554030901E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 60 4.013379E-4 12.696211 1 TAGGACC 725 0.0 12.210485 4 GTAGGAC 1645 0.0 11.574004 3 TGTAGGA 1705 0.0 11.334485 2 GGCGAGG 800 0.0 11.031 19 GGACGTG 1440 0.0 10.971878 6 TGGCGAG 1545 0.0 10.935518 18 AGGACGT 1465 0.0 10.850926 5 GTCCTAC 1660 0.0 10.841493 1 ATATGGC 2585 0.0 10.465082 15 AGGACCT 1210 0.0 10.462954 5 GACGTGA 885 0.0 10.40267 7 TAGGACG 1570 0.0 10.367749 4 TCCTACA 1805 0.0 10.284603 2 GGACCTG 1200 0.0 10.2316065 6 TTTATAC 310 0.0 10.133788 3 CGGGACT 85 6.4753165E-4 10.078864 4 TATGGCG 1630 0.0 10.074345 16 TTAGACA 285 0.0 10.019924 4 TGGAATA 2040 0.0 10.002871 11 >>END_MODULE