FastQCFastQC Report
Thu 26 May 2016
SRR1513177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78992
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC35604.506785497265546No Hit
CCCATGTACTCTGCGTTGATACCAC31313.9636925258253997No Hit
GAGTACATGGGAAGCAGTGGTATCA13891.7584059145229896No Hit
TATCAACGCAGAGTACATGGGAAGC10011.2672169333603402No Hit
CATGTACTCTGCGTTGATACCACTG9691.2267065019242456No Hit
GCGTTGATACCACTGCTTCCCATGT9681.2254405509418675No Hit
GTATCAACGCAGAGTACATGGGAAG9131.1558132469110796No Hit
GTATCAACGCAGAGTACTTTTTTTT8201.0380798055499292No Hit
ACGCAGAGTACATGGGAAGCAGTGG7300.9241442171359125No Hit
GGTATCAACGCAGAGTACATGGGAA7100.8988251974883532No Hit
ACTCTGCGTTGATACCACTGCTTCC6520.8254000405104314No Hit
TATCAACGCAGAGTACTTTTTTTTT5040.638039295118493No Hit
GGTATCAACGCAGAGTACTTTTTTT5010.6342414421713591No Hit
GTACTCTGCGTTGATACCACTGCTT4200.5316994125987442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4050.5127101478630747No Hit
GGGAAGCAGTGGTATCAACGCAGAG3770.47726352035649183No Hit
GTGGTATCAACGCAGAGTACATGGG3750.47473161839173583No Hit
GCTTCCCATGTACTCTGCGTTGATA3480.4405509418675309No Hit
ATACCACTGCTTCCCATGTACTCTG3400.43042333400850724No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3250.41143406927283777No Hit
GCAGTGGTATCAACGCAGAGTACAT3050.38611504962527854No Hit
CTGCTTCCCATGTACTCTGCGTTGA3000.3797852947133887No Hit
CAGTGGTATCAACGCAGAGTACATG2810.3557322260482074No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2770.3506684221186956No Hit
TACCACTGCTTCCCATGTACTCTGC2740.34687056917156167No Hit
GTTGATACCACTGCTTCCCATGTAC2610.33041320640064814No Hit
GATACCACTGCTTCCCATGTACTCT2560.3240834514887584No Hit
GCAGAGTACATGGGAAGCAGTGGTA2550.32281750050638036No Hit
CCACTGCTTCCCATGTACTCTGCGT2470.3126898926473567No Hit
CATGGGAAGCAGTGGTATCAACGCA2250.2848389710350415No Hit
GAACAAAAAAAAAAAAAAAAAAAAA2170.27471136317601785No Hit
CTTCCCATGTACTCTGCGTTGATAC2170.27471136317601785No Hit
AAGCAGTGGTATCAACGCAGAGTAC2150.2721794612112619No Hit
ACATGGGAAGCAGTGGTATCAACGC2110.2671156572817501No Hit
ATGGGAAGCAGTGGTATCAACGCAG2110.2671156572817501No Hit
CCATGTACTCTGCGTTGATACCACT2010.25445614745797046No Hit
GTACATGGGAAGCAGTGTTATCAAC1810.22913712781041118No Hit
TGATACCACTGCTTCCCATGTACTC1790.2266052258456553No Hit
CCCATGTACTCTGCGTTTATACCAC1780.2253392748632773No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1770.22407332388089934No Hit
GTACATGGGAAGCAGTTGTATCAAC1700.21521166700425362No Hit
ATCAACGCAGAGTACATGGGAAGCA1680.21267976503949768No Hit
GTACATGGTAAGCAGTGGTATCAAC1520.19242454932145026No Hit
GTACTGGTTCACTATCGGTCAGTCA1390.17596718655053675No Hit
TTCCCATGTACTCTGCGTTGATACC1320.16710552967389103No Hit
AAAAAGTACTCTGCGTTGATACCAC1250.1582438727972453No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1230.15571197083248936No Hit
GGAAGCAGTGGTATCAACGCAGAGT1060.134190804132064No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1000.12659509823779622No Hit
ACCATGTACTCTGCGTTGATACCAC970.12279724529066234No Hit
GAGTACATGTGAAGCAGTGGTATCA920.11646749037877253No Hit
GAGTACATGGGAATCAGTGGTATCA870.11013773546688273No Hit
AAAGTACTCTGCGTTGATACCACTG800.10127607859023698No Hit
CCCCATGTACTCTGCGTTGATACCA800.10127607859023698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTAC401.27547155E-518.97338511
TTACATG307.706629E-418.97338314
ATTACAT350.002165935716.26290113
GCAGATT508.65097E-515.1787069
CGCAGAT508.65097E-515.1787068
CAGATTA508.65097E-515.17870610
GATTACA400.00526935614.23003812
CATGGAA758.960924E-713.9847134
ACATGGA801.8532046E-613.1106683
CATGGGT657.636022E-411.7354244
ATGGGGA1053.8947444E-59.9700315
GAAGCAG8150.08.2644819
GGAAGCA8600.07.9423478
AAGCAGT9050.07.65224910
CAGTGGT8650.07.56741913
AGTGGTA8750.07.48093414
AGCAGTG9000.07.378538611
AGTACTT3352.7284841E-117.36280613
GAGTACT3352.7284841E-117.36280612
GTACATG18350.07.34536031
Produced by FastQC (version 0.11.5)