##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513177_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78992
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.61548004861252	31.0	28.0	33.0	10.0	34.0
2	27.386469515900345	31.0	27.0	34.0	10.0	34.0
3	27.64479947336439	31.0	27.0	34.0	10.0	34.0
4	30.257823577071097	35.0	30.0	37.0	10.0	37.0
5	30.76001367227061	35.0	31.0	37.0	10.0	37.0
6	30.739264735669433	35.0	31.0	37.0	8.0	37.0
7	30.717730909459185	35.0	31.0	37.0	2.0	37.0
8	30.292143508203363	35.0	31.0	37.0	2.0	37.0
9	31.112226554587807	37.0	30.0	39.0	2.0	39.0
10	30.81481669029775	37.0	30.0	39.0	2.0	39.0
11	31.20524863277294	37.0	31.0	39.0	2.0	39.0
12	31.264760988454526	37.0	31.0	39.0	2.0	39.0
13	31.169536155560056	37.0	31.0	39.0	2.0	39.0
14	31.27776230504355	38.0	28.0	41.0	2.0	41.0
15	31.61412548106137	38.0	30.0	41.0	2.0	41.0
16	31.412218958881912	38.0	30.0	40.0	2.0	41.0
17	31.205830970224834	38.0	30.0	40.0	2.0	41.0
18	30.922587097427588	38.0	27.0	40.0	2.0	41.0
19	31.4756177840794	38.0	30.0	40.0	2.0	41.0
20	31.7870543852542	38.0	31.0	41.0	2.0	41.0
21	31.827387583552763	39.0	31.0	41.0	2.0	41.0
22	31.5806537370873	38.0	30.0	41.0	2.0	41.0
23	31.230048612517724	38.0	30.0	41.0	2.0	41.0
24	31.44232327324286	38.0	30.0	41.0	2.0	41.0
25	31.04892900546891	38.0	30.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.8939495578933254
1101	2	0.3918644229841739
1101	3	-0.07729613552470482
1101	4	0.5519308804755063
1101	5	0.5985811415870543
1101	6	0.17400504402593597
1101	7	0.03981077296488067
1101	8	0.493827818388457
1101	9	0.6211089885428258
1101	10	0.663080350458273
1101	11	0.7138321606606652
1101	12	0.9489259182604606
1101	13	0.8046561050777328
1101	14	1.009009621056233
1101	15	0.7699746020835185
1101	16	1.0081965210219543
1101	17	1.3752885860257287
1101	18	1.5867298329268422
1101	19	1.1657606867021038
1101	20	0.785381584040298
1101	21	0.8600306491702199
1101	22	1.1909603277887406
1101	23	1.0134000191134689
1101	24	0.901727469364431
1101	25	0.873901258197403
1102	1	0.28718251757837265
1102	2	0.4059296030870918
1102	3	0.759753607185413
1102	4	0.28880909659900666
1102	5	0.33031356011192514
1102	6	0.3501628484856276
1102	7	0.7977867592427543
1102	8	0.7451142677881144
1102	9	1.303613276707491
1102	10	2.059649818725859
1102	11	1.4459076323587823
1102	12	1.8352037913307875
1102	13	0.8218087637398277
1102	14	1.6163852642809324
1102	15	1.0684308628039325
1102	16	1.8464469498384268
1102	17	2.336352050862775
1102	18	2.6649459564259885
1102	19	1.8336852150039853
1102	20	0.9121397315531645
1102	21	0.5279551774721014
1102	22	1.476895147685827
1102	23	2.1001924719436573
1102	24	1.8500979667915303
1102	25	2.003232304509581
1103	1	1.339663887484825
1103	2	1.3174564779879958
1103	3	1.2829024893798007
1103	4	1.6254984893372324
1103	5	1.5858233111821676
1103	6	1.785234990550002
1103	7	1.5479849517272974
1103	8	2.0469217832471287
1103	9	2.759535268145573
1103	10	3.1707961640564406
1103	11	2.1583699757867194
1103	12	2.4688648029052267
1103	13	2.488766112717151
1103	14	3.251790369719334
1103	15	3.537354281227909
1103	16	2.9311147792343313
1103	17	2.984837860280887
1103	18	4.064422728266798
1103	19	2.6674413589710078
1103	20	2.8821730278905626
1103	21	2.7902827500105545
1103	22	2.9267128106000477
1103	23	2.6713832123907792
1103	24	3.242598363176498
1103	25	2.8709982788238335
1104	1	1.1250159593480547
1104	2	1.6570681847396607
1104	3	1.0857132688640085
1104	4	1.576382291133811
1104	5	1.7593961886089957
1104	6	1.1092715017061856
1104	7	1.0482747228081415
1104	8	1.3433575989527178
1104	9	0.7847453521791898
1104	10	1.3350239241259843
1104	11	0.27072871238480545
1104	12	0.962562281896826
1104	13	0.4621796160495215
1104	14	1.2325205928430698
1104	15	0.8264009343719216
1104	16	0.7342153297993868
1104	17	1.2950378023266715
1104	18	1.5571060084754365
1104	19	1.069208962564172
1104	20	0.9435320542597303
1104	21	1.1772720284805587
1104	22	1.1826531177573933
1104	23	1.670296570837607
1104	24	1.6923230806496932
1104	25	1.791456117131574
1105	1	1.6585094585001166
1105	2	1.7568420682896893
1105	3	1.331049617083341
1105	4	1.9181007761536009
1105	5	1.4945572965796003
1105	6	1.2804133897785448
1105	7	1.400168448076652
1105	8	1.6011303519204958
1105	9	1.637204368572629
1105	10	1.8343173102198236
1105	11	1.1908813409885326
1105	12	0.9174803146837114
1105	13	1.3091270439749039
1105	14	1.9832272067492305
1105	15	1.9346541847958925
1105	16	1.2986585180413321
1105	17	1.525959226860305
1105	18	1.5140025601995717
1105	19	1.4903508506365277
1105	20	1.504159527408408
1105	21	1.5862601573669366
1105	22	1.822703993958637
1105	23	2.0002852650151084
1105	24	1.8969584678741143
1105	25	2.3892514817443526
1106	1	0.4364852247153692
1106	2	0.736528454604727
1106	3	0.9191465522223297
1106	4	1.0116146749419102
1106	5	0.946802485460573
1106	6	0.7079971388875954
1106	7	1.081708006166462
1106	8	0.6456064745149384
1106	9	0.4108323087009289
1106	10	0.8670329196282331
1106	11	0.43991911718240573
1106	12	0.1995188036359572
1106	13	-0.14119369729380793
1106	14	1.7244246408190769
1106	15	0.8043619538621805
1106	16	0.7271688530377638
1106	17	1.0744980721917337
1106	18	1.3183503862865251
1106	19	1.1527172084412278
1106	20	1.0980298053841686
1106	21	0.5861176056919533
1106	22	1.6332528179073158
1106	23	1.7742695843308596
1106	24	1.6155614614592935
1106	25	1.633585052663804
1107	1	1.5727171087733396
1107	2	2.085803816923569
1107	3	1.315885682657111
1107	4	1.9996581532027804
1107	5	1.8576720506779623
1107	6	2.3474898925107865
1107	7	1.5284471366012475
1107	8	2.143070242630877
1107	9	1.7264120188458598
1107	10	1.2039894413673622
1107	11	1.163832160660668
1107	12	0.8542289485634917
1107	13	1.6584439838656095
1107	14	2.3802217422683576
1107	15	2.3275503596592806
1107	16	2.0112268240522617
1107	17	2.3737734345105785
1107	18	2.7640025601995717
1107	19	2.2240940200354338
1107	20	1.77629067494939
1107	21	1.785030649170217
1107	22	2.3136876005160154
1107	23	2.955066685780139
1107	24	2.653242620879581
1107	25	2.8132951975913443
1108	1	1.0437553601394605
1108	2	1.5765141994372307
1108	3	0.4540004367554715
1108	4	1.4836745466454033
1108	5	1.5027540178910783
1108	6	0.813200275024446
1108	7	1.2526274644700983
1108	8	0.9126057617565628
1108	9	0.8994994505398459
1108	10	2.02284190038376
1108	11	0.9695698655786984
1108	12	1.2863327737001065
1108	13	1.2353565521716234
1108	14	2.5897845837984086
1108	15	1.8117033651237584
1108	16	1.7084945836151029
1108	17	1.4931723416144003
1108	18	2.1779369864290814
1108	19	1.834613145718496
1108	20	1.4139955929821788
1108	21	1.307571632776778
1108	22	1.8964744857619174
1108	23	1.803563953539701
1108	24	1.8477781400052677
1108	25	2.217120334203365
1109	1	1.2743044103606422
1109	2	1.431835562955314
1109	3	1.550409492180922
1109	4	2.3103724389170637
1109	5	2.263227606233521
1109	6	1.2082041782250705
1109	7	1.6875741207282253
1109	8	1.5768004013610373
1109	9	2.133951701385538
1109	10	2.1301799175578395
1109	11	2.678911525740034
1109	12	2.1728797422142883
1109	13	1.8342376346592602
1109	14	3.0841899962366064
1109	15	1.7735821056910233
1109	16	2.5048776177030554
1109	17	3.0221861329232773
1109	18	3.125113671310686
1109	19	1.9975067184481325
1109	20	2.049306547965262
1109	21	2.199713188852755
1109	22	2.443052679881095
1109	23	2.3165746222880728
1109	24	2.615147382784343
1109	25	2.589485673781823
1110	1	0.959276248558286
1110	2	1.5191371502569027
1110	3	1.2166921342700157
1110	4	1.290787185460843
1110	5	1.538317211968284
1110	6	1.142920000037666
1110	7	1.0663503624076967
1110	8	1.6653820705878672
1110	9	2.091196965082414
1110	10	1.9443120220125252
1110	11	1.660606354209051
1110	12	1.2690138948000502
1110	13	1.729142908596799
1110	14	2.2683937852791054
1110	15	2.2318514349280925
1110	16	2.754237576740433
1110	17	2.415171283972942
1110	18	1.7881961085866678
1110	19	1.8528574608956525
1110	20	1.740806803981645
1110	21	1.3374500040089288
1110	22	1.3153005037418204
1110	23	0.6085613094360518
1110	24	1.2457157391591522
1110	25	0.9788865954408053
1111	1	1.2981764263586406
1111	2	1.7632316384458804
1111	3	1.3274998558854598
1111	4	1.8553668146201048
1111	5	1.4832626018590673
1111	6	1.610153934505533
1111	7	1.9987227271524226
1111	8	1.0178077754392802
1111	9	1.9973437773760416
1111	10	1.7209185752256317
1111	11	1.8846983023929482
1111	12	1.3346095259913149
1111	13	0.17294529620157206
1111	14	2.073003894499326
1111	15	1.1167892048036343
1111	16	1.0248751180155118
1111	17	2.1452957442218654
1111	18	2.124238780672016
1111	19	1.0326898205603676
1111	20	1.4676292576265517
1111	21	1.2387708066505354
1111	22	1.6077820887049903
1111	23	2.2173370269874866
1111	24	1.645893539514752
1111	25	2.1985970348616846
1112	1	-0.08200925938586323
1112	2	0.4295849114509309
1112	3	-0.020308347193633836
1112	4	0.3754044218594963
1112	5	0.41662727455856086
1112	6	-0.24417677415588201
1112	7	-0.16222450518980125
1112	8	-0.3406362250308099
1112	9	-0.5212247970745416
1112	10	-0.38332399146845475
1112	11	-0.12646634680201885
1112	12	-0.2949004046703365
1112	13	-0.5752624837960774
1112	14	0.663555075601689
1112	15	0.5996896631418629
1112	16	0.11918702305723627
1112	17	0.14218139470958135
1112	18	0.26027121691598865
1112	19	0.047850238940906564
1112	20	-0.0699779817670283
1112	21	-0.32504397769545434
1112	22	-0.5616855338123443
1112	23	-0.6373462495432456
1112	24	-0.46516533931942305
1112	25	-0.5011326631051745
1113	1	-0.9497635113816969
1113	2	-0.6979171133089892
1113	3	-0.43353292199405047
1113	4	-0.39782246695225965
1113	5	-0.8078318252910286
1113	6	-1.2247969291946426
1113	7	-1.1996536385925545
1113	8	-0.973402625586175
1113	9	-0.8586655005339878
1113	10	-1.01655319429155
1113	11	-0.5427569866261557
1113	12	-1.2719338421341817
1113	13	-0.7933002021808981
1113	14	-0.4837317461037358
1113	15	-0.7708992527438205
1113	16	-1.029083253467121
1113	17	-0.06382346471422906
1113	18	-0.5828966646066291
1113	19	-1.2164098559335592
1113	20	-0.5213837436552637
1113	21	-1.2740778779615667
1113	22	-0.45801782584057804
1113	23	-1.1315612211966055
1113	24	-0.33280389074832684
1113	25	-0.14639472488927652
1114	1	-2.009739031577535
1114	2	-1.4282312707957274
1114	3	-1.9846782271173247
1114	4	-2.009176433263388
1114	5	-3.8224031372919605
1114	6	-2.067485044832573
1114	7	-2.157455119037852
1114	8	-2.370150308747565
1114	9	-1.7641268282719373
1114	10	-2.0715744684070714
1114	11	-2.3816565611438456
1114	12	-2.370144485020468
1114	13	-1.9544006276632082
1114	14	-6.89283590184192
1114	15	-3.8571614197893425
1114	16	-2.643409767426437
1114	17	-2.0045473674944354
1114	18	-9.933365860853058
1114	19	-4.752284426079854
1114	20	-3.4928822573814386
1114	21	-3.5091422831606103
1114	22	-2.7490943543712056
1114	23	-2.211412010961716
1114	24	-2.577835073356006
1114	25	-2.4669053036617896
1115	1	-0.3781600842091173
1115	2	0.7747762480012632
1115	3	0.4288556074691421
1115	4	-2.903913275368648
1115	5	-0.04796704706639687
1115	6	0.300936007799006
1115	7	0.7899133020147779
1115	8	0.9982707438840137
1115	9	0.9426400890212925
1115	10	0.544966884976386
1115	11	0.06195246141254529
1115	12	0.8103066427990804
1115	13	0.3538700490285187
1115	14	-3.261256954473499
1115	15	-1.0225749536239306
1115	16	0.23628948069386269
1115	17	0.9202646625807525
1115	18	1.0816717331319055
1115	19	0.9244073032434521
1115	20	0.7552380433704435
1115	21	0.7389780175912719
1115	22	-0.7190191664012815
1115	23	0.7585128010683562
1115	24	1.241713798824442
1115	25	1.1947488316765558
1116	1	1.19176472782096
1116	2	1.1977085788283297
1116	3	1.7551713969428278
1116	4	1.2532295817742067
1116	5	1.2505291935351082
1116	6	1.53439465441555
1116	7	1.3034471366012461
1116	8	2.141879766440404
1116	9	2.063316780750622
1116	10	2.239703727081654
1116	11	2.363832160660664
1116	12	2.3697051390396844
1116	13	1.9715392219608532
1116	14	3.392126504173117
1116	15	2.6251694072783245
1116	16	3.0302744430998807
1116	17	3.5356781964153363
1116	18	3.335431131628141
1116	19	3.180046400987816
1116	20	2.440576389235101
1116	21	2.552887792027363
1116	22	2.385116171944585
1116	23	1.8919714476849023
1116	24	1.8984807161176747
1116	25	2.3823428166389604
1201	1	1.2621907929838656
1201	2	1.2384353958709369
1201	3	1.7408856826571117
1201	4	2.024658153202779
1201	5	1.3304790682218268
1201	6	1.203191646896748
1201	7	1.7823945050222996
1201	8	2.777719365437896
1201	9	2.1882541241090117
1201	10	2.68030523084105
1201	11	2.585761985222071
1201	12	2.3529131590898054
1201	13	2.2536194224621013
1201	14	3.7951340229701067
1201	15	3.0977257982557695
1201	16	3.3077180521224356
1201	17	2.9641243117035607
1201	18	2.303476244410099
1201	19	2.3429536691582378
1201	20	2.939448569686231
1201	21	2.78283766671408
1201	22	3.2847402320949577
1201	23	3.2397158085871567
1201	24	2.8707864805287073
1201	25	3.1132951975913414
1202	1	1.7605958966521271
1202	2	1.63731896843872
1202	3	1.2863402281116585
1202	4	1.697385425930051
1202	5	1.4440356870415982
1202	6	1.3921868622077511
1202	7	1.33071986387397
1202	8	1.912009636570275
1202	9	1.9847453521791927
1202	10	2.2721712595491823
1202	11	1.6683776152061185
1202	12	1.87619864553319
1202	13	1.941019741441373
1202	14	2.7453732574198675
1202	15	2.033610965719884
1202	16	2.27183288465832
1202	17	3.0752885860257315
1202	18	2.8503661965632077
1202	19	2.5566697776111944
1202	20	2.5035634022221167
1202	21	1.7145761037156717
1202	22	2.2455057823341953
1202	23	2.804309110022565
1202	24	2.7926365602735217
1202	25	1.8920830763792225
1203	1	-0.2580521219958918
1203	2	-0.10479447367472261
1203	3	-0.21210577033433964
1203	4	0.1827778113224383
1203	5	0.49827034127625325
1203	6	0.03360100362189655
1203	7	0.19490012805423618
1203	8	0.5676428922035299
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2211	11	0.17320716066066666
2211	12	0.07193728189682602
2211	13	0.3735481505322795
2211	14	0.827617575601689
2211	15	0.439008692992612
2211	16	-6.478877342614407
2211	17	-1.92883073215609
2211	18	0.3108775601995717
2211	19	-0.4404893132978991
2211	20	0.05910317494938866
2211	21	0.683468149170217
2211	22	0.40275010051601257
2211	23	0.45558751911347173
2211	24	0.4261592875462483
2211	25	-6.318996469075323
2212	1	-2.3683681625444883
2212	2	-5.271560524161703
2212	3	-2.914153077032811
2212	4	-4.746659676254584
2212	5	-2.3194597322677737
2212	6	-3.077510107489214
2212	7	-2.7655451114607708
2212	8	-2.7253406100822986
2212	9	-2.664867050921586
2212	10	-2.225855519872944
2212	11	-2.8063228780990244
2212	12	-2.8920888808938727
2212	13	-3.173145163421207
2212	14	-9.049623218971956
2212	15	-7.3290387876275425
2212	16	-5.455439842614407
2212	17	-9.815761449210353
2212	18	-5.350338525071745
2212	19	-3.8288129567087523
2212	20	-3.978748084740534
2212	21	-3.68493059113986
2212	22	-3.566544957623524
2212	23	-2.992026337475675
2212	24	-3.4723387744692573
2212	25	-3.0223637171373383
2213	1	-0.28218485201097465
2213	2	-0.6499804968019198
2213	3	-0.22970255263700423
2213	4	-0.43857714091486955
2213	5	-0.2702691257926233
2213	6	-0.22947089180294
2213	7	-0.14214109869287128
2213	8	0.30532514459166293
2213	9	0.6670982933556608
2213	10	-3.610716440985577
2213	11	-0.9729325452216848
2213	12	-0.25802595339728995
2213	13	0.026581238767573012
2213	14	1.325319781484044
2213	15	0.30941310475731854
2213	16	0.5813248632679482
2213	17	0.642400885490968
2213	18	0.7934143249054557
2213	19	0.3334077455256299
2213	20	-0.21341520740355335
2213	21	0.21150123740551408
2213	22	-0.6510182818369294
2213	23	-0.7645411573571153
2213	24	-0.4433260065714002
2213	25	-0.07543029260473588
2214	1	-2.295512057893326
2214	2	-2.0277378497430973
2214	3	-1.2434893173428883
2214	4	-2.100341846797221
2214	5	-4.417327949322036
2214	6	-3.189489274155882
2214	7	-2.8043653633987553
2214	8	-2.485575590702453
2214	9	-1.8324421478208102
2214	10	-5.611635558632635
2214	11	-2.5455428393393333
2214	12	-2.521812718103174
2214	13	-1.7280143494677205
2214	14	-1.148944924398311
2214	15	-1.857866307007388
2214	16	-1.6585648426144068
2214	17	-1.92101823215609
2214	18	-1.2438099398004283
2214	19	-1.846739313297899
2214	20	-1.6752718250506113
2214	21	-2.668094350829783
2214	22	-2.3316248994839874
2214	23	-1.7475374808865283
2214	24	-2.3941532124537517
2214	25	-1.8268089690753229
2215	1	-2.746581136840696
2215	2	-5.127479391096482
2215	3	-3.3380616857639396
2215	4	-2.1520335761205303
2215	5	-2.2735309568408333
2215	6	-2.300567751599491
2215	7	-2.285274667910034
2215	8	-2.7009773764167377
2215	9	-8.21525464782081
2215	10	-3.725709806752935
2215	11	-3.028273102497227
2215	12	-3.25736253013325
2215	13	-3.1348517554827566
2215	14	-7.94546748078929
2215	15	-5.293251645353251
2215	16	-7.846417286223428
2215	17	-4.057178946441805
2215	18	-3.6025387931838857
2215	19	-4.301156606530981
2215	20	-3.8913784979829416
2215	21	-4.080570854589183
2215	22	-9.598718414521581
2215	23	-10.955772913217356
2215	24	-7.186857629746985
2215	25	-5.166153423962541
2216	1	-1.4439495578933261
2216	2	-1.413295282175529
2216	3	-1.3469521551807269
2216	4	-5.705071576526951
2216	5	-2.130165787159875
2216	6	-1.9603929903720996
2216	7	-1.5465528633987553
2216	8	-6.239587415026776
2216	9	-2.951741134307298
2216	10	-1.4338483964704736
2216	11	-1.6658975690690632
2216	12	-1.4833836640491214
2216	13	-1.453943403521773
2216	14	-0.3702287081820934
2216	15	-1.3756027934938757
2216	16	-0.8270614642360279
2216	17	-0.38343377269663037
2216	18	-6.823835277638267
2216	19	-3.392347421406008
2216	20	-2.8439795953208815
2216	21	-2.0291585400189724
2216	22	-1.9268529400245278
2216	23	-1.4555188998054476
2216	24	-1.6963069286699692
2216	25	-0.9925606582645123
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6625.0
3	0.0
4	30.0
5	321.0
6	725.0
7	561.0
8	505.0
9	335.0
10	342.0
11	376.0
12	361.0
13	312.0
14	280.0
15	230.0
16	193.0
17	209.0
18	257.0
19	363.0
20	518.0
21	670.0
22	821.0
23	974.0
24	998.0
25	1042.0
26	1031.0
27	1098.0
28	1131.0
29	1251.0
30	1499.0
31	1710.0
32	2216.0
33	2745.0
34	3732.0
35	4938.0
36	7088.0
37	10307.0
38	23198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.33420001268312	17.123470099562432	10.572642526475997	21.96968736127846
2	13.559752259112601	24.142044877652555	36.17118489186719	26.12701797136765
3	22.172301756523506	32.42841912884557	25.14849223271398	20.250786881916945
4	12.084250155806824	20.136601248998385	30.47072739529145	37.308421199903336
5	10.807209036679783	43.00038075897957	32.04340652367051	14.149003680670136
6	30.99139022537351	31.11800455811598	19.722714611294	18.16789060521651
7	25.44727079350207	34.80798693323542	20.4750629914281	19.269679281834414
8	30.369713851608005	30.893897189161812	20.79007343631299	17.946315522917196
9	31.130188153663045	15.134594444022387	17.182000050647016	36.553217351667556
10	17.525355483241956	32.113146865543136	28.593134710105474	21.768362941109437
11	28.888523095623984	28.984754862236628	16.700010129659642	25.42671191247974
12	26.593050349508662	32.22571168068078	25.03292472900415	16.148313240806402
13	31.300890222745636	22.921652800466006	20.22439184996644	25.553065126821917
14	22.3705204508041	29.45675573002406	24.43586171964037	23.736862099531468
15	34.31021162882002	23.73636950822579	21.208475284641395	20.744943578312792
16	18.097215715569963	29.597741171703873	30.900619151926463	21.404423960799708
17	24.81414404944338	29.999113463949644	25.591762813612128	19.594979672994846
18	25.462558413434138	24.85594518951914	29.28840089662247	20.39309550042425
19	22.38191718374066	31.85513486133975	30.130429276940614	15.632518677978979
20	25.431520293801054	27.01576647881973	26.605458114354462	20.947255113024756
21	21.569869050935893	28.54284339302449	30.30065094602467	19.586636610014942
22	22.755598338230822	28.782044786705846	21.15969196473807	27.30266491032526
23	18.175140885202303	30.62369404166403	27.285506236940414	23.915658836193252
24	21.768647169835216	35.96443453700303	22.754043544893797	19.512874748267958
25	21.609230340565116	25.868510708360247	22.537583748116063	29.98467520295857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	494.0
1	494.0
2	421.0
3	348.0
4	348.0
5	348.0
6	614.0
7	880.0
8	880.0
9	880.0
10	773.0
11	666.0
12	666.0
13	666.0
14	633.0
15	600.0
16	600.0
17	600.0
18	707.0
19	814.0
20	814.0
21	814.0
22	1104.0
23	1394.0
24	1394.0
25	1394.0
26	1909.0
27	2424.0
28	2424.0
29	2424.0
30	2836.5
31	3249.0
32	3249.0
33	3249.0
34	3522.5
35	3796.0
36	3796.0
37	3796.0
38	4043.5
39	4291.0
40	4291.0
41	4291.0
42	5760.0
43	7229.0
44	7229.0
45	7229.0
46	13649.5
47	20070.0
48	20070.0
49	20070.0
50	17483.5
51	14897.0
52	14897.0
53	14897.0
54	11103.5
55	7310.0
56	7310.0
57	7310.0
58	5686.5
59	4063.0
60	4063.0
61	4063.0
62	3539.0
63	3015.0
64	3015.0
65	3015.0
66	2364.0
67	1713.0
68	1713.0
69	1713.0
70	1343.5
71	974.0
72	974.0
73	974.0
74	729.0
75	484.0
76	484.0
77	484.0
78	336.0
79	188.0
80	188.0
81	188.0
82	127.5
83	67.0
84	67.0
85	67.0
86	42.5
87	18.0
88	18.0
89	18.0
90	11.5
91	5.0
92	5.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	1.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18609479440956045
2	0.25319019647559243
3	0.25319019647559243
4	0.46713591249746805
5	0.2557220984403484
6	0.015191411788535548
7	0.01645736277091351
8	0.015191411788535548
9	0.017723313753291472
10	0.018989264735669434
11	0.0202552157180474
12	0.030382823577071096
13	0.029116872594693134
14	0.027850921612315175
15	0.04177638241847276
16	0.01645736277091351
17	0.04177638241847276
18	0.03671257848896091
19	0.027850921612315175
20	0.034180676524204985
21	0.03797852947133887
22	0.05063803929511849
23	0.034180676524204985
24	0.04937208831274053
25	0.04430828438322868
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	78992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.58583147660523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.88387560190482	40.392697994733645
2	8.592939423767591	8.178043346161637
3	2.7428236984224106	3.9155863884950377
4	1.2344036819282236	2.349605023293498
5	0.595919018861901	1.4178651002633178
6	0.3618079757375828	1.0330160016204173
7	0.19420575168267312	0.6469009519951388
8	0.19686610444544947	0.7494429815677537
9	0.13567799090159355	0.5810715009114847
>10	0.8539732368512064	7.522280737289852
>50	0.07981058288329032	2.550891229491594
>100	0.09311234669717204	10.59980757545068
>500	0.023943174864987097	8.566690297751672
>1k	0.010641411051105377	11.496100870974276
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3560	4.506785497265546	No Hit
CCCATGTACTCTGCGTTGATACCAC	3131	3.9636925258253997	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1389	1.7584059145229896	No Hit
TATCAACGCAGAGTACATGGGAAGC	1001	1.2672169333603402	No Hit
CATGTACTCTGCGTTGATACCACTG	969	1.2267065019242456	No Hit
GCGTTGATACCACTGCTTCCCATGT	968	1.2254405509418675	No Hit
GTATCAACGCAGAGTACATGGGAAG	913	1.1558132469110796	No Hit
GTATCAACGCAGAGTACTTTTTTTT	820	1.0380798055499292	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	730	0.9241442171359125	No Hit
GGTATCAACGCAGAGTACATGGGAA	710	0.8988251974883532	No Hit
ACTCTGCGTTGATACCACTGCTTCC	652	0.8254000405104314	No Hit
TATCAACGCAGAGTACTTTTTTTTT	504	0.638039295118493	No Hit
GGTATCAACGCAGAGTACTTTTTTT	501	0.6342414421713591	No Hit
GTACTCTGCGTTGATACCACTGCTT	420	0.5316994125987442	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	405	0.5127101478630747	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	377	0.47726352035649183	No Hit
GTGGTATCAACGCAGAGTACATGGG	375	0.47473161839173583	No Hit
GCTTCCCATGTACTCTGCGTTGATA	348	0.4405509418675309	No Hit
ATACCACTGCTTCCCATGTACTCTG	340	0.43042333400850724	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	325	0.41143406927283777	No Hit
GCAGTGGTATCAACGCAGAGTACAT	305	0.38611504962527854	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	300	0.3797852947133887	No Hit
CAGTGGTATCAACGCAGAGTACATG	281	0.3557322260482074	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	277	0.3506684221186956	No Hit
TACCACTGCTTCCCATGTACTCTGC	274	0.34687056917156167	No Hit
GTTGATACCACTGCTTCCCATGTAC	261	0.33041320640064814	No Hit
GATACCACTGCTTCCCATGTACTCT	256	0.3240834514887584	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	255	0.32281750050638036	No Hit
CCACTGCTTCCCATGTACTCTGCGT	247	0.3126898926473567	No Hit
CATGGGAAGCAGTGGTATCAACGCA	225	0.2848389710350415	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	217	0.27471136317601785	No Hit
CTTCCCATGTACTCTGCGTTGATAC	217	0.27471136317601785	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	215	0.2721794612112619	No Hit
ACATGGGAAGCAGTGGTATCAACGC	211	0.2671156572817501	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	211	0.2671156572817501	No Hit
CCATGTACTCTGCGTTGATACCACT	201	0.25445614745797046	No Hit
GTACATGGGAAGCAGTGTTATCAAC	181	0.22913712781041118	No Hit
TGATACCACTGCTTCCCATGTACTC	179	0.2266052258456553	No Hit
CCCATGTACTCTGCGTTTATACCAC	178	0.2253392748632773	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	177	0.22407332388089934	No Hit
GTACATGGGAAGCAGTTGTATCAAC	170	0.21521166700425362	No Hit
ATCAACGCAGAGTACATGGGAAGCA	168	0.21267976503949768	No Hit
GTACATGGTAAGCAGTGGTATCAAC	152	0.19242454932145026	No Hit
GTACTGGTTCACTATCGGTCAGTCA	139	0.17596718655053675	No Hit
TTCCCATGTACTCTGCGTTGATACC	132	0.16710552967389103	No Hit
AAAAAGTACTCTGCGTTGATACCAC	125	0.1582438727972453	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	123	0.15571197083248936	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	106	0.134190804132064	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	100	0.12659509823779622	No Hit
ACCATGTACTCTGCGTTGATACCAC	97	0.12279724529066234	No Hit
GAGTACATGTGAAGCAGTGGTATCA	92	0.11646749037877253	No Hit
GAGTACATGGGAATCAGTGGTATCA	87	0.11013773546688273	No Hit
AAAGTACTCTGCGTTGATACCACTG	80	0.10127607859023698	No Hit
CCCCATGTACTCTGCGTTGATACCA	80	0.10127607859023698	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0025319019647559245	0.0
2	0.0	0.0	0.0	0.0025319019647559245	0.0
3	0.0	0.0	0.0	0.0025319019647559245	0.0
4	0.0	0.0	0.0	0.0025319019647559245	0.0
5	0.0	0.0	0.0	0.0025319019647559245	0.0
6	0.0	0.0	0.0	0.0025319019647559245	0.0
7	0.0	0.0	0.0	0.0025319019647559245	0.0
8	0.0012659509823779622	0.0	0.0	0.0025319019647559245	0.0
9	0.0012659509823779622	0.0	0.0	0.0025319019647559245	0.0
10	0.0012659509823779622	0.0	0.0	0.0025319019647559245	0.0
11	0.0012659509823779622	0.0	0.0	0.0025319019647559245	0.0
12	0.0012659509823779622	0.0	0.0	0.0025319019647559245	0.0
13	0.0012659509823779622	0.0	0.0	0.0025319019647559245	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATTAC	40	1.27547155E-5	18.973385	11
TTACATG	30	7.706629E-4	18.973383	14
ATTACAT	35	0.0021659357	16.262901	13
GCAGATT	50	8.65097E-5	15.178706	9
CGCAGAT	50	8.65097E-5	15.178706	8
CAGATTA	50	8.65097E-5	15.178706	10
GATTACA	40	0.005269356	14.230038	12
CATGGAA	75	8.960924E-7	13.984713	4
ACATGGA	80	1.8532046E-6	13.110668	3
CATGGGT	65	7.636022E-4	11.735424	4
ATGGGGA	105	3.8947444E-5	9.970031	5
GAAGCAG	815	0.0	8.264481	9
GGAAGCA	860	0.0	7.942347	8
AAGCAGT	905	0.0	7.652249	10
CAGTGGT	865	0.0	7.567419	13
AGTGGTA	875	0.0	7.480934	14
AGCAGTG	900	0.0	7.3785386	11
AGTACTT	335	2.7284841E-11	7.362806	13
GAGTACT	335	2.7284841E-11	7.362806	12
GTACATG	1835	0.0	7.3453603	1
>>END_MODULE