FastQCFastQC Report
Thu 26 May 2016
SRR1513177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78992
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC40505.127101478630747No Hit
GTACATGGGAAGCAGTGGTATCAAC39334.978985213692526No Hit
GTATCAACGCAGAGTACTTTTTTTT16002.0255215718047395No Hit
GAGTACATGGGAAGCAGTGGTATCA15111.912851934373101No Hit
TATCAACGCAGAGTACTTTTTTTTT13291.682448855580312No Hit
TATCAACGCAGAGTACATGGGAAGC12631.5988960907433662No Hit
CATGTACTCTGCGTTGATACCACTG12331.5609175612720274No Hit
GGTATCAACGCAGAGTACTTTTTTT10281.3013976098845452No Hit
ACGCAGAGTACATGGGAAGCAGTGG10271.3001316589021672No Hit
GCGTTGATACCACTGCTTCCCATGT9801.240631962730403No Hit
GTATCAACGCAGAGTACATGGGAAG9421.1925258254000406No Hit
GGTATCAACGCAGAGTACATGGGAA8411.0646647761798662No Hit
ACTCTGCGTTGATACCACTGCTTCC8051.0190905408142596No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7460.9443994328539599No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6060.7671662953210452No Hit
CTTATACACATCTCCGAGCCCACGA5180.6557626088717845No Hit
ATACCACTGCTTCCCATGTACTCTG4850.6139862264533117No Hit
GGGAAGCAGTGGTATCAACGCAGAG4230.5354972655458781No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4210.5329653635811221No Hit
GTACTCTGCGTTGATACCACTGCTT4170.5279015596516102No Hit
TACCACTGCTTCCCATGTACTCTGC4170.5279015596516102No Hit
GCTTCCCATGTACTCTGCGTTGATA4070.5152420498278307No Hit
GTGGTATCAACGCAGAGTACATGGG3970.502582540004051No Hit
CAGTGGTATCAACGCAGAGTACATG3690.46713591249746805No Hit
GCAGAGTACATGGGAAGCAGTGGTA3620.45827425562082236No Hit
CTGCTTCCCATGTACTCTGCGTTGA3430.43422118695564105No Hit
CATGGGAAGCAGTGGTATCAACGCA3290.4164978732023496No Hit
GATACCACTGCTTCCCATGTACTCT3200.4051043143609479No Hit
CCACTGCTTCCCATGTACTCTGCGT3060.38738100060765646No Hit
AAGCAGTGGTATCAACGCAGAGTAC3030.3835831476605226No Hit
ACATGGGAAGCAGTGGTATCAACGC3020.3823171966781446No Hit
CCATGTACTCTGCGTTGATACCACT3010.38105124569576665No Hit
GCAGTGGTATCAACGCAGAGTACAT2940.3721895888191209No Hit
ATACACATCTCCGAGCCCACGAGAC2900.36712578488960906No Hit
GTTGATACCACTGCTTCCCATGTAC2800.35446627506582945No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2660.336742961312538No Hit
ATGGGAAGCAGTGGTATCAACGCAG2420.3063601377354669No Hit
ACACATCTCCGAGCCCACGAGACGG2170.27471136317601785No Hit
ATCAACGCAGAGTACATGGGAAGCA2150.2721794612112619No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT2130.26964755924650596No Hit
CTTCCCATGTACTCTGCGTTGATAC2090.26458375531699413No Hit
ATCAACGCAGAGTACTTTTTTTTTT1970.24939234352845857No Hit
TGATACCACTGCTTCCCATGTACTC1830.23166902977516712No Hit
TTCCCATGTACTCTGCGTTGATACC1740.22027547093376545No Hit
GTACATGGTAAGCAGTGGTATCAAC1680.21267976503949768No Hit
AAAAAGTACTCTGCGTTGATACCAC1560.19748835325096212No Hit
GTGGTATCAACGCAGAGTACTTTTT1480.18736074539193842No Hit
GGAAGCAGTGGTATCAACGCAGAGT1230.15571197083248936No Hit
ACCATGTACTCTGCGTTGATACCAC1180.14938221592059955No Hit
GTACTGGTTCACTATCGGTCAGTCA1170.14811626493822158No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1160.14685031395584364No Hit
AAAGTACTCTGCGTTGATACCACTG1100.13925460806157586No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT920.11646749037877253No Hit
CCCCATGTACTCTGCGTTGATACCA900.11393558841401662No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT870.11013773546688273No Hit
TCAACGCAGAGTACATGGGAAGCAG860.10887178448450475No Hit
GGATACCACGTGTCCCGCCCTACTC850.10760583350212681No Hit
TGGGAAGCAGTGGTATCAACGCAGA830.10507393153737087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC451.5830519E-619.0553071
TATACAC602.4693629E-514.2733343
TTATACA602.4693629E-514.2733342
ATACACA803.63045E-410.7054
TACACAT856.349914E-410.0752945
CCATGTA9500.09.2150652
TGCGTTG9750.09.05338312
CTGCGTT9800.09.00719311
GAACATG850.00735955558.9331298
CTCTGCG10250.08.7969539
TGATACC10250.08.79695317
TCTGCGT10200.08.65396910
TTGATAC10250.08.61175416
CGTTGAT10250.08.61175414
GATACCA10700.08.51569418
TACTCTG10600.08.422287
GTTGATA10500.08.40671315
GCGTTGA10500.08.40671313
CCCATGT9550.08.3803451
ATGTACT10600.08.3485544