##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513177_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 78992 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.553663662143002 31.0 30.0 34.0 25.0 34.0 2 29.48560613733036 31.0 30.0 34.0 25.0 34.0 3 29.63652015393964 31.0 30.0 34.0 25.0 34.0 4 32.8353377557221 35.0 33.0 37.0 26.0 37.0 5 32.675562082236176 35.0 33.0 37.0 25.0 37.0 6 32.371404699210046 37.0 33.0 37.0 20.0 37.0 7 32.07491897913713 37.0 33.0 37.0 17.0 37.0 8 32.02333147660523 37.0 33.0 37.0 17.0 37.0 9 33.28735821348997 39.0 34.0 39.0 13.0 39.0 10 33.19323475795017 39.0 34.0 39.0 13.0 39.0 11 33.1708907231112 39.0 34.0 39.0 11.0 39.0 12 33.119758962932956 39.0 34.0 39.0 11.0 39.0 13 33.02496455337249 39.0 34.0 39.0 10.0 39.0 14 33.965553473769496 39.0 34.0 41.0 10.0 41.0 15 33.90281294308284 39.0 34.0 41.0 10.0 41.0 16 33.70633735061779 39.0 33.0 41.0 10.0 41.0 17 33.87331628519344 39.0 34.0 41.0 10.0 41.0 18 33.84595908446425 39.0 34.0 41.0 8.0 41.0 19 33.83071703463642 39.0 34.0 41.0 7.0 41.0 20 33.97004759975694 39.0 34.0 41.0 2.0 41.0 21 33.912737998784685 39.0 34.0 41.0 2.0 41.0 22 33.89622999797448 39.0 34.0 41.0 2.0 41.0 23 33.821057828640875 39.0 34.0 41.0 2.0 41.0 24 33.71418624670853 39.0 34.0 41.0 2.0 41.0 25 33.67111859428803 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.37502100891304835 1101 2 0.044348188640956465 1101 3 -0.15325653255953497 1101 4 -0.3480624904582257 1101 5 -0.4732746102181409 1101 6 -0.0766202184345417 1101 7 -0.31159892462484606 1101 8 -0.803410164258267 1101 9 -1.2018365703468383 1101 10 -0.7499908254354608 1101 11 -0.8138835275822984 1101 12 -0.5287485585597693 1101 13 -0.5549547710792098 1101 14 -1.09462537336627 1101 15 -0.6686827271173428 1101 16 -0.7409593915696746 1101 17 -0.7208413458265284 1101 18 -0.5750881553206 1101 19 -0.6320415371664012 1101 20 -1.1013431959011726 1101 21 -0.9960457350638166 1101 22 -0.7402061924042371 1101 23 -0.6535161872407755 1101 24 -0.39929664406563603 1101 25 -0.21248737568278386 1102 1 -0.7007499969061861 1102 2 -0.08909949575012632 1102 3 -0.3379906663502723 1102 4 -0.4286799861700672 1102 5 -0.212725725140956 1102 6 -1.1546648153470613 1102 7 -0.9685457513829938 1102 8 -1.6874581917025218 1102 9 -1.7611847693176763 1102 10 -1.0866975149723395 1102 11 -1.0725456202066574 1102 12 -1.2474449565014503 1102 13 -0.6103578585577694 1102 14 -0.9853629376887412 1102 15 -0.9681681473574812 1102 16 -0.9661737998029523 1102 17 -0.8539459770443685 1102 18 -0.9001310369672524 1102 19 -1.4011667515746353 1102 20 -0.7510859060212383 1102 21 -0.587812458906015 1102 22 -0.9470672558690723 1102 23 -0.8233275079954936 1102 24 0.04924537994808986 1102 25 -0.5411323156484755 1103 1 0.7841080972748813 1103 2 0.7823924972734986 1103 3 0.8451391893655966 1103 4 0.5319986248970068 1103 5 0.38058329658094436 1103 6 0.9432422720161853 1103 7 1.2655592113568197 1103 8 0.7793247478884062 1103 9 1.2154285175064885 1103 10 0.5866019935102997 1103 11 0.8389812546942466 1103 12 0.9914959028611605 1103 13 0.8270925933210975 1103 14 1.1254662996665772 1103 15 1.4945716655488184 1103 16 1.1326846114860913 1103 17 1.2308011293453518 1103 18 0.5994725780636685 1103 19 0.7959951320467411 1103 20 0.8395276979108957 1103 21 0.5807304299476428 1103 22 0.8214439221870578 1103 23 1.21203003124662 1103 24 1.3719027447808116 1103 25 1.3117295838282956 1104 1 0.4292109660085828 1104 2 0.33933251465976255 1104 3 0.5439221508260452 1104 4 0.2107149390088523 1104 5 0.6049385559260614 1104 6 0.29328573767831045 1104 7 0.08887129481089318 1104 8 0.2100694595661814 1104 9 0.2588217368631973 1104 10 0.6727364472918111 1104 11 0.23893778903212137 1104 12 0.6287749524120159 1104 13 0.41322704710260894 1104 14 -0.18835578088978622 1104 15 0.3711291851083658 1104 16 0.7789465645431761 1104 17 1.2547072435151634 1104 18 0.9178585531433541 1104 19 1.2928800929276463 1104 20 1.065114484349614 1104 21 0.953013826064705 1104 22 1.2760947480346374 1104 23 1.113725192069566 1104 24 0.6539948919845244 1104 25 0.6690173265115718 1105 1 0.6543381565116917 1105 2 0.1872692020537734 1105 3 -0.04878559366236601 1105 4 0.4932191786922928 1105 5 0.20004878769542245 1105 6 0.3891025833538393 1105 7 0.2614264106955737 1105 8 0.5664855138341345 1105 9 0.6905628335279772 1105 10 0.9063430046688623 1105 11 1.089842254832007 1105 12 0.6724436918128092 1105 13 0.90912868644687 1105 14 1.2845102451136086 1105 15 1.3906317289184287 1105 16 0.7149273446449342 1105 17 0.35561170931504904 1105 18 1.034449847660028 1105 19 0.5448586118648961 1105 20 1.2397894419527802 1105 21 1.1365921188855097 1105 22 1.118661169741813 1105 23 0.8956641406280781 1105 24 0.947946276947782 1105 25 0.9392264842278024 1106 1 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0.7548937137692775 1107 14 0.7015867478458517 1107 15 0.6343475759129618 1107 16 0.38555575994547553 1107 17 0.5314313814461968 1107 18 1.1052148749824369 1107 19 0.783109977985113 1107 20 0.375171955613979 1107 21 0.45243911342102905 1107 22 0.924945322747277 1107 23 1.1131504794258902 1107 24 1.1560063862374008 1107 25 1.3966035334081255 1108 1 1.3674529106100515 1108 2 1.4987446118898369 1108 3 1.4184275210917292 1108 4 1.2637109819709806 1108 5 1.1836553450724665 1108 6 1.528446845648915 1108 7 1.2614264106955737 1108 8 1.3123871531783955 1108 9 1.8135136532001113 1108 10 1.447326611226238 1108 11 1.6226291400779047 1108 12 1.7134272983701848 1108 13 1.4501122930042456 1108 14 1.6123790975726209 1108 15 1.587353040393836 1108 16 1.993615869235093 1108 17 1.8965953158724247 1108 18 1.5918268968403595 1108 19 2.331743857766533 1108 20 2.534871409165895 1108 21 2.095608512328134 1108 22 1.9465300222008324 1108 23 2.280910042267422 1108 24 2.333192178587126 1108 25 2.644144517014688 1109 1 0.27663844274640326 1109 2 -0.18119293690017102 1109 3 0.2541027747997724 1109 4 0.45496781257207175 1109 5 0.8801164431729092 1109 6 0.10664096482664576 1109 7 0.4863808733549497 1109 8 0.40164034079223754 1109 9 0.03755736904710005 1109 10 0.6528951774505387 1109 11 0.8501857364869636 1109 12 0.7865472567360499 1109 13 0.9806873645629253 1109 14 0.6170629383220358 1109 15 1.2041888457542314 1109 16 0.6915081408978594 1109 17 1.187383762398575 1109 18 1.2409291606967159 1109 19 1.5434274383025723 1109 20 1.013664019106045 1109 21 0.9079946689765848 1109 22 0.36859611639807355 1109 23 0.473467939743351 1109 24 0.9564032116342247 1109 25 0.5759686127732095 1110 1 -0.04965443999808983 1110 2 -0.41122365886637624 1110 3 -0.10457618065337826 1110 4 0.15926888784088788 1110 5 0.12217993523640303 1110 6 -0.032631948639817665 1110 7 -0.44283059617909615 1110 8 0.05128191785316716 1110 9 0.6289552185094678 1110 10 0.5840267698724588 1110 11 0.33098450760831355 1110 12 0.5005769546367134 1110 13 -0.04645037225223092 1110 14 -0.3126605639821065 1110 15 -0.014577155269833497 1110 16 -0.22439047661366374 1110 17 -0.4758266830699327 1110 18 0.070806272831895 1110 19 0.23364761239371745 1110 20 -0.028322668041937504 1110 21 -0.0722920693746687 1110 22 0.08838618296233136 1110 23 0.48358058695277606 1110 24 0.4320816550546027 1110 25 -0.3421061440112254 1111 1 0.2941987477082826 1111 2 0.13976694311482873 1111 3 0.09849722549343554 1111 4 -0.0975256257481334 1111 5 0.6871405651576623 1111 6 -0.3371785577330364 1111 7 0.08174395297000103 1111 8 -0.12023192517451875 1111 9 -0.05824315588728268 1111 10 -0.4709643401097665 1111 11 0.465858777356857 1111 12 0.8434776404380813 1111 13 0.9663766534018237 1111 14 1.1181877977146115 1111 15 1.46453130294708 1111 16 1.1071436864546627 1111 17 1.0072187830209955 1111 18 0.7452411217015964 1111 19 0.7694616840218629 1111 20 0.7782559451152906 1111 21 0.42986693494334105 1111 22 0.800667107524184 1111 23 0.46459404897969847 1111 24 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12 1.1383276085163985 2216 13 0.656470290345851 2216 14 1.1045146757737783 2216 15 0.2609241524895367 2216 16 0.4907359401256599 2216 17 0.9685935436083568 2216 18 0.599359019126581 2216 19 0.3308577257328622 2216 20 0.13035213579416194 2216 21 0.35469136567328263 2216 22 0.2956660434680032 2216 23 0.5829702992457086 2216 24 0.20082620605721502 2216 25 -0.13853160172700996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3702.0 3 0.0 4 61.0 5 476.0 6 857.0 7 500.0 8 386.0 9 315.0 10 273.0 11 253.0 12 191.0 13 150.0 14 168.0 15 137.0 16 152.0 17 151.0 18 176.0 19 290.0 20 366.0 21 466.0 22 532.0 23 602.0 24 682.0 25 714.0 26 754.0 27 760.0 28 873.0 29 994.0 30 1225.0 31 1546.0 32 1908.0 33 2598.0 34 3418.0 35 4467.0 36 6636.0 37 11250.0 38 30963.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.84188896076715 18.19323414132958 12.626051219605008 24.338825678298264 2 12.052616253307507 23.127856482712346 35.40582627520985 29.4137009887703 3 19.27332573743512 31.24066337511077 27.297126218508673 22.188884668945434 4 11.328303033372157 20.54236086494151 28.2485947232491 39.88074137843723 5 10.147846908137282 41.38319840877707 33.9161060646371 14.552848618448557 6 32.08661417322835 30.928371856743713 19.353568707137413 17.631445262890526 7 23.17754169146417 32.8947868258772 22.34054676914895 21.58712471350968 8 31.04448130468364 30.407587019005533 20.847841776719804 17.70008989959102 9 29.00224087508071 12.7375390887108 18.314406168103613 39.94581386810488 10 17.53199893653386 31.181080430956992 29.464342233532097 21.822578398977047 11 28.49022685841604 26.06972858010938 18.40312943082844 27.036915130646143 12 25.28483897103504 30.63601377354669 27.16983998379583 16.909307271622442 13 33.10715009114847 18.96141381405712 22.859276888798867 25.072159205995543 14 22.834246939524753 26.17260194199339 25.656087402362292 25.337063716119555 15 34.870619809600974 21.32874215110391 22.83648977111606 20.964148268179056 16 18.255013165890215 25.46966781446222 33.247670650192426 23.027648369455132 17 23.87681709973155 28.004862482905335 28.469584156409866 19.64873626095325 18 26.69722795598161 20.347740195271445 31.83101802018565 21.124013828561296 19 22.080717034636418 28.324387279724526 33.63505165080008 15.959844034838971 20 24.007494429815676 22.804840996556614 31.562689892647356 21.62497468098035 21 20.908711118988236 25.610512590041907 33.82157460976567 19.659201681204188 22 21.586003468749603 25.639629831246598 25.33959565013736 27.43477104986644 23 17.548235174963285 26.0951030536284 31.02749784777434 25.329163923633967 24 21.88829248531497 32.10325096212275 27.312892444804536 18.695564107757747 25 20.716284133635476 21.975921307490726 26.42706131078224 30.88073324809156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 355.0 1 355.0 2 331.5 3 308.0 4 308.0 5 308.0 6 576.0 7 844.0 8 844.0 9 844.0 10 705.5 11 567.0 12 567.0 13 567.0 14 421.0 15 275.0 16 275.0 17 275.0 18 499.0 19 723.0 20 723.0 21 723.0 22 1144.0 23 1565.0 24 1565.0 25 1565.0 26 2398.0 27 3231.0 28 3231.0 29 3231.0 30 3449.0 31 3667.0 32 3667.0 33 3667.0 34 3735.0 35 3803.0 36 3803.0 37 3803.0 38 3666.0 39 3529.0 40 3529.0 41 3529.0 42 4311.0 43 5093.0 44 5093.0 45 5093.0 46 12953.0 47 20813.0 48 20813.0 49 20813.0 50 18423.5 51 16034.0 52 16034.0 53 16034.0 54 11693.0 55 7352.0 56 7352.0 57 7352.0 58 5608.5 59 3865.0 60 3865.0 61 3865.0 62 3661.0 63 3457.0 64 3457.0 65 3457.0 66 2639.5 67 1822.0 68 1822.0 69 1822.0 70 1375.0 71 928.0 72 928.0 73 928.0 74 732.5 75 537.0 76 537.0 77 537.0 78 358.5 79 180.0 80 180.0 81 180.0 82 106.0 83 32.0 84 32.0 85 32.0 86 20.5 87 9.0 88 9.0 89 9.0 90 6.0 91 3.0 92 3.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19622240226858417 2 0.006329754911889811 3 0.002531901964755925 4 0.00506380392951185 5 0.07469110796029978 6 0.3190196475592465 7 0.024053068665181282 8 0.018989264735669434 9 0.006329754911889811 10 0.006329754911889811 11 0.0 12 0.0 13 0.0 14 0.0012659509823779625 15 0.0 16 0.0 17 0.025319019647559244 18 0.031648774559449054 19 0.0 20 0.0 21 0.0012659509823779625 22 0.0012659509823779625 23 0.00506380392951185 24 0.0 25 0.0012659509823779625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 78992.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.75708932550131 #Duplication Level Percentage of deduplicated Percentage of total 1 81.66585007349339 31.651306461413814 2 10.550383798791442 8.178043346161637 3 3.3937612281561327 3.945969212072108 4 1.4437367303609343 2.2382013368442375 5 0.7643312101910829 1.481162649382216 6 0.43442756818552997 1.010228883937614 7 0.27764167891556424 0.7532408345148875 8 0.20578147966682997 0.638039295118493 9 0.11758941695247428 0.4101681182904598 >10 0.8851869998366813 6.590540814259673 >50 0.09145843540748 2.4027749645533723 >100 0.11758941695247428 12.327830666396597 >500 0.02286460885187 6.8842414421713585 >1k 0.02939735423811857 21.488251974883532 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 4050 5.127101478630747 No Hit GTACATGGGAAGCAGTGGTATCAAC 3933 4.978985213692526 No Hit GTATCAACGCAGAGTACTTTTTTTT 1600 2.0255215718047395 No Hit GAGTACATGGGAAGCAGTGGTATCA 1511 1.912851934373101 No Hit TATCAACGCAGAGTACTTTTTTTTT 1329 1.682448855580312 No Hit TATCAACGCAGAGTACATGGGAAGC 1263 1.5988960907433662 No Hit CATGTACTCTGCGTTGATACCACTG 1233 1.5609175612720274 No Hit GGTATCAACGCAGAGTACTTTTTTT 1028 1.3013976098845452 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1027 1.3001316589021672 No Hit GCGTTGATACCACTGCTTCCCATGT 980 1.240631962730403 No Hit GTATCAACGCAGAGTACATGGGAAG 942 1.1925258254000406 No Hit GGTATCAACGCAGAGTACATGGGAA 841 1.0646647761798662 No Hit ACTCTGCGTTGATACCACTGCTTCC 805 1.0190905408142596 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 746 0.9443994328539599 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 606 0.7671662953210452 No Hit CTTATACACATCTCCGAGCCCACGA 518 0.6557626088717845 No Hit ATACCACTGCTTCCCATGTACTCTG 485 0.6139862264533117 No Hit GGGAAGCAGTGGTATCAACGCAGAG 423 0.5354972655458781 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 421 0.5329653635811221 No Hit GTACTCTGCGTTGATACCACTGCTT 417 0.5279015596516102 No Hit TACCACTGCTTCCCATGTACTCTGC 417 0.5279015596516102 No Hit GCTTCCCATGTACTCTGCGTTGATA 407 0.5152420498278307 No Hit GTGGTATCAACGCAGAGTACATGGG 397 0.502582540004051 No Hit CAGTGGTATCAACGCAGAGTACATG 369 0.46713591249746805 No Hit GCAGAGTACATGGGAAGCAGTGGTA 362 0.45827425562082236 No Hit CTGCTTCCCATGTACTCTGCGTTGA 343 0.43422118695564105 No Hit CATGGGAAGCAGTGGTATCAACGCA 329 0.4164978732023496 No Hit GATACCACTGCTTCCCATGTACTCT 320 0.4051043143609479 No Hit CCACTGCTTCCCATGTACTCTGCGT 306 0.38738100060765646 No Hit AAGCAGTGGTATCAACGCAGAGTAC 303 0.3835831476605226 No Hit ACATGGGAAGCAGTGGTATCAACGC 302 0.3823171966781446 No Hit CCATGTACTCTGCGTTGATACCACT 301 0.38105124569576665 No Hit GCAGTGGTATCAACGCAGAGTACAT 294 0.3721895888191209 No Hit ATACACATCTCCGAGCCCACGAGAC 290 0.36712578488960906 No Hit GTTGATACCACTGCTTCCCATGTAC 280 0.35446627506582945 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 266 0.336742961312538 No Hit ATGGGAAGCAGTGGTATCAACGCAG 242 0.3063601377354669 No Hit ACACATCTCCGAGCCCACGAGACGG 217 0.27471136317601785 No Hit ATCAACGCAGAGTACATGGGAAGCA 215 0.2721794612112619 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 213 0.26964755924650596 No Hit CTTCCCATGTACTCTGCGTTGATAC 209 0.26458375531699413 No Hit ATCAACGCAGAGTACTTTTTTTTTT 197 0.24939234352845857 No Hit TGATACCACTGCTTCCCATGTACTC 183 0.23166902977516712 No Hit TTCCCATGTACTCTGCGTTGATACC 174 0.22027547093376545 No Hit GTACATGGTAAGCAGTGGTATCAAC 168 0.21267976503949768 No Hit AAAAAGTACTCTGCGTTGATACCAC 156 0.19748835325096212 No Hit GTGGTATCAACGCAGAGTACTTTTT 148 0.18736074539193842 No Hit GGAAGCAGTGGTATCAACGCAGAGT 123 0.15571197083248936 No Hit ACCATGTACTCTGCGTTGATACCAC 118 0.14938221592059955 No Hit GTACTGGTTCACTATCGGTCAGTCA 117 0.14811626493822158 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 116 0.14685031395584364 No Hit AAAGTACTCTGCGTTGATACCACTG 110 0.13925460806157586 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 92 0.11646749037877253 No Hit CCCCATGTACTCTGCGTTGATACCA 90 0.11393558841401662 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 87 0.11013773546688273 No Hit TCAACGCAGAGTACATGGGAAGCAG 86 0.10887178448450475 No Hit GGATACCACGTGTCCCGCCCTACTC 85 0.10760583350212681 No Hit TGGGAAGCAGTGGTATCAACGCAGA 83 0.10507393153737087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0025319019647559245 0.0 0.0 0.0 0.0 2 0.0025319019647559245 0.0 0.0 0.0 0.0 3 0.0025319019647559245 0.0 0.0 0.0 0.0 4 0.0025319019647559245 0.0 0.0 0.0 0.0 5 0.0025319019647559245 0.0 0.0 0.0 0.0 6 0.0025319019647559245 0.0 0.0 0.0 0.0 7 0.0025319019647559245 0.0 0.0 0.0 0.0 8 0.0025319019647559245 0.0 0.0 0.0 0.0 9 0.0025319019647559245 0.0 0.0 0.0 0.0 10 0.0025319019647559245 0.0 0.0 0.0 0.0 11 0.0025319019647559245 0.0 0.0 0.0 0.0 12 0.0025319019647559245 0.0 0.0 0.0 0.0 13 0.0025319019647559245 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 45 1.5830519E-6 19.055307 1 TATACAC 60 2.4693629E-5 14.273334 3 TTATACA 60 2.4693629E-5 14.273334 2 ATACACA 80 3.63045E-4 10.705 4 TACACAT 85 6.349914E-4 10.075294 5 CCATGTA 950 0.0 9.215065 2 TGCGTTG 975 0.0 9.053383 12 CTGCGTT 980 0.0 9.007193 11 GAACATG 85 0.0073595555 8.933129 8 CTCTGCG 1025 0.0 8.796953 9 TGATACC 1025 0.0 8.796953 17 TCTGCGT 1020 0.0 8.653969 10 TTGATAC 1025 0.0 8.611754 16 CGTTGAT 1025 0.0 8.611754 14 GATACCA 1070 0.0 8.515694 18 TACTCTG 1060 0.0 8.42228 7 GTTGATA 1050 0.0 8.406713 15 GCGTTGA 1050 0.0 8.406713 13 CCCATGT 955 0.0 8.380345 1 ATGTACT 1060 0.0 8.348554 4 >>END_MODULE