FastQCFastQC Report
Thu 26 May 2016
SRR1513176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810889
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC26740.14766228079136823No Hit
CCCATGTACTCTGCGTTGATACCAC21920.12104551963151801No Hit
GTCCTACAGTGGACATTTCTAAATT20480.11309362418127229No Hit
GTATCAACGCAGAGTACTTTTTTTT20480.11309362418127229No Hit
GTCCTAAAGTGTGTATTTCTCATTT18890.10431340628829265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4200.012.4797864
CGGGCCG550.003096282512.07651210
CGCAATT550.003096282512.07651211
TATACTG2050.011.5978415
CGCAGAT1800.011.5963258
GTCCTAT1850.011.3575731
GGCGAGG5500.011.21514419
GTCTACG600.005648046311.1424671
TATCGCA600.005932286411.069228
GTATTAT1657.2759576E-1110.9977591
TGGCGAA951.3768209E-510.98811618
GTCCTAC12900.010.883341
ATTCCGT700.001507893810.8430186
AGGACCT8150.010.6187845
CTAATAC1909.094947E-1210.533473
TGTAGGA12750.010.4718872
TTAGGAC8500.010.427253
GTAGGAC11950.010.3676713
GGACGTG10400.010.2174596
GTATTAA2150.010.2170121
Produced by FastQC (version 0.11.5)