##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513176_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1810889 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.675086104117923 31.0 30.0 34.0 25.0 34.0 2 29.540630044138542 31.0 30.0 34.0 23.0 34.0 3 29.723082971954657 31.0 30.0 34.0 25.0 34.0 4 32.80695614143109 37.0 33.0 37.0 25.0 37.0 5 32.9607159798309 37.0 35.0 37.0 26.0 37.0 6 33.34896948404899 37.0 35.0 37.0 28.0 37.0 7 33.331777375642574 37.0 35.0 37.0 28.0 37.0 8 33.212352054709044 37.0 35.0 37.0 27.0 37.0 9 34.48710163902923 39.0 35.0 39.0 26.0 39.0 10 34.059357586246314 39.0 34.0 39.0 23.0 39.0 11 34.52555788897055 39.0 35.0 39.0 27.0 39.0 12 34.52341087719899 39.0 35.0 39.0 27.0 39.0 13 34.56066550738339 39.0 35.0 39.0 27.0 39.0 14 34.80472740184517 40.0 34.0 41.0 18.0 41.0 15 35.23836855820539 40.0 35.0 41.0 25.0 41.0 16 35.09121762846867 40.0 35.0 41.0 23.0 41.0 17 34.71146492137287 39.0 34.0 41.0 18.0 41.0 18 34.44882817224026 39.0 34.0 41.0 17.0 41.0 19 35.0862962887289 40.0 34.0 41.0 24.0 41.0 20 35.29820381039368 40.0 36.0 41.0 25.0 41.0 21 35.343848794708016 40.0 36.0 41.0 25.0 41.0 22 35.03188157860587 40.0 35.0 41.0 22.0 41.0 23 34.5772965653886 40.0 34.0 41.0 18.0 41.0 24 34.82742343677608 40.0 35.0 41.0 21.0 41.0 25 34.46875761021244 40.0 34.0 41.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8528633186897459 1101 2 1.1222760866330184 1101 3 1.0131898436212907 1101 4 1.3848095571812493 1101 5 1.1887904744604043 1101 6 0.8803707258085254 1101 7 0.8821341227156694 1101 8 0.9887498677638149 1101 9 1.3195466206646103 1101 10 1.6896308006510594 1101 11 1.1829328407357877 1101 12 1.1963353285240999 1101 13 1.0029666851145151 1101 14 2.1521489185121894 1101 15 1.6093265140579476 1101 16 1.643216367340493 1101 17 2.0634654807400565 1101 18 2.1156200868958166 1101 19 1.6059424358213121 1101 20 1.2519745873974273 1101 21 1.3559944663256474 1101 22 1.6560169089861603 1101 23 1.9052568605049771 1101 24 1.4987361723734551 1101 25 1.8205633172425237 1102 1 0.8906866562133438 1102 2 1.1074603456544807 1102 3 0.9750052083771052 1102 4 1.3031602147825225 1102 5 1.283043841315859 1102 6 1.0904635199043113 1102 7 1.0176855368174884 1102 8 1.0227231176522977 1102 9 1.3777876442607635 1102 10 1.6222486349598668 1102 11 1.1717317665861202 1102 12 1.186302347082922 1102 13 1.0466442847684831 1102 14 2.0351594212088244 1102 15 1.5733307854249219 1102 16 1.6461247717506353 1102 17 1.9289070017612175 1102 18 2.200130805864198 1102 19 1.5871676265234314 1102 20 1.4532737385308252 1102 21 1.290209557587815 1102 22 1.6354063242864854 1102 23 1.8806213121885165 1102 24 1.6601824671428318 1102 25 1.8598690173547183 1103 1 0.5757661947391846 1103 2 0.9236161015866351 1103 3 0.7809408209781843 1103 4 1.1308579218214305 1103 5 1.004739590508791 1103 6 0.7970895583740827 1103 7 0.7657339589275409 1103 8 0.7397894400290213 1103 9 1.0223118609639457 1103 10 1.3773173097273812 1103 11 0.8196586567712387 1103 12 0.6472531559962178 1103 13 0.7898158629945726 1103 14 1.7650465213292534 1103 15 1.2882228407747078 1103 16 1.36118723629464 1103 17 1.7782933771259763 1103 18 1.8800407603785843 1103 19 1.4427971500471983 1103 20 1.1051645366823024 1103 21 1.1733696025416762 1103 22 1.2751829160419916 1103 23 1.6684622786690113 1103 24 1.3784157549877918 1103 25 1.6291107226148611 1104 1 0.8530946641575774 1104 2 1.0323572473545752 1104 3 0.8842782896619532 1104 4 1.2369168090501645 1104 5 1.2031825832967584 1104 6 0.9053640137640357 1104 7 0.9175862339287448 1104 8 0.8790280214894395 1104 9 1.330823268031402 1104 10 1.5671965071652636 1104 11 1.1186930733026301 1104 12 1.118730340044607 1104 13 1.0219550314648203 1104 14 2.0602170160547004 1104 15 1.4273596869624114 1104 16 1.511074917054735 1104 17 2.0213109529709 1104 18 2.1298783674680735 1104 19 1.477924078929803 1104 20 1.2974777101486126 1104 21 1.290024972017676 1104 22 1.5539368904125084 1104 23 1.9095510560169302 1104 24 1.5682198621053445 1104 25 1.790919462724439 1105 1 0.7363630902487301 1105 2 0.9493412706770208 1105 3 0.8787389242289656 1105 4 1.1928760633138396 1105 5 0.9516375227155365 1105 6 0.8084816068845626 1105 7 0.7001763004317425 1105 8 0.852123654653731 1105 9 1.0071357347328274 1105 10 1.4264844063773126 1105 11 0.8950772949162555 1105 12 0.7487647490784965 1105 13 0.7738082033313134 1105 14 1.5863093481137298 1105 15 1.2779259168005623 1105 16 1.3868856818629425 1105 17 1.621069448992273 1105 18 1.3705879892775599 1105 19 1.196591721474114 1105 20 1.0324448393005454 1105 21 0.8640274236325567 1105 22 1.1396433715052794 1105 23 1.5746195266213192 1105 24 1.3461350899823898 1105 25 1.5215938952314048 1106 1 0.7817467670200244 1106 2 0.9413728857142125 1106 3 0.9978082714800678 1106 4 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1107 17 1.4114479839417484 1107 18 1.6516845187257942 1107 19 1.1278956392410606 1107 20 0.9112153544182959 1107 21 0.820388654827255 1107 22 1.0154605938707277 1107 23 1.2186394883129452 1107 24 1.1074291909605591 1107 25 1.3835392440017245 1108 1 0.6482631530268357 1108 2 0.7979024995133095 1108 3 0.6590691944297582 1108 4 1.1127966665360205 1108 5 1.05487099353752 1108 6 0.6662298433084004 1108 7 0.5192083172660489 1108 8 0.5793403007307347 1108 9 0.9201620324922501 1108 10 1.2249484937950328 1108 11 0.7748002586377396 1108 12 0.6431096473216655 1108 13 0.6336818398195163 1108 14 1.553333580887724 1108 15 1.1714501054917932 1108 16 1.2495879181869256 1108 17 1.6130813067249719 1108 18 1.8007408396266626 1108 19 1.0994572074308309 1108 20 0.948724099899799 1108 21 0.9574522136551948 1108 22 1.082206316913826 1108 23 1.430525847027603 1108 24 1.0163637414428095 1108 25 1.4180995128628098 1109 1 0.47194484826818695 1109 2 0.7082025182586875 1109 3 0.6427298165703164 1109 4 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2215 15 -4.884073525344803 2215 16 -9.413712646018631 2215 17 -4.660049229619883 2215 18 -3.203331118370624 2215 19 -3.719759972920528 2215 20 -3.595101076827465 2215 21 -3.639410536231555 2215 22 -9.360912094422773 2215 23 -10.560674064285525 2215 24 -6.8393655404521425 2215 25 -5.145109275756919 2216 1 -1.8053730299296298 2216 2 -1.8943167465094746 2216 3 -1.7014477307597282 2216 4 -7.161307612867372 2216 5 -3.635172414808082 2216 6 -2.779436775176965 2216 7 -2.259539661377584 2216 8 -8.258940801055289 2216 9 -4.0255228798328275 2216 10 -2.2433577676668506 2216 11 -2.2034054544606505 2216 12 -2.3206854887754673 2216 13 -2.1244681787001696 2216 14 -1.490030046441916 2216 15 -2.160254699690107 2216 16 -1.887514021411782 2216 17 -1.6922435557324533 2216 18 -7.498264291682453 2216 19 -4.911791923047158 2216 20 -4.220115167262534 2216 21 -3.329371478899894 2216 22 -2.9512029772601522 2216 23 -2.325210965598302 2216 24 -2.637501227435081 2216 25 -2.1570411916081156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72576.0 3 0.0 4 602.0 5 3596.0 6 4513.0 7 4092.0 8 3835.0 9 3524.0 10 3429.0 11 3218.0 12 3158.0 13 3143.0 14 3255.0 15 3254.0 16 3316.0 17 3550.0 18 4195.0 19 5024.0 20 6504.0 21 8253.0 22 10423.0 23 11993.0 24 14059.0 25 15942.0 26 18285.0 27 20500.0 28 23250.0 29 27870.0 30 33252.0 31 39979.0 32 51555.0 33 67082.0 34 88949.0 35 120199.0 36 172005.0 37 253122.0 38 699387.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73898565715455 18.38373334365856 12.352768727839944 25.524512271346943 2 14.511136369802 20.231325562687168 41.07033610360763 24.187201963903203 3 18.523569079493736 24.882625209004637 29.635030838565367 26.958774872936253 4 11.592035274511414 16.739970308696357 38.62199508695469 33.04599932983754 5 12.84947811157943 37.88091100297913 36.01021797346848 13.259392911972956 6 32.02093069111158 36.71757761604642 18.21096055501459 13.05053113782741 7 28.053844292962264 31.368133731443525 22.43849120214034 18.13953077345387 8 25.29820834734831 36.13025340736555 20.842080612494566 17.72945763279157 9 26.71729964331202 14.80045246019709 19.956245243147645 38.52600265334324 10 14.235570284451809 28.444076222038113 35.383485225075944 21.936868268434136 11 34.47509076156868 22.268384706434034 24.468037512020075 18.78848701997721 12 23.40259702956734 24.50579327167395 31.49229773262571 20.599311966133 13 29.05029198160836 21.635417380141718 25.561371374534076 23.752919263715842 14 20.72873939331004 21.479009773455132 28.394278877648983 29.39797195558585 15 23.523243872135364 29.93641903661223 23.224426050461485 23.315911040790926 16 22.40732701244845 27.48761948317211 27.566658160757267 22.53839534362217 17 21.871305550706353 27.841867073852523 27.99655055829689 22.290276817144232 18 21.763385027550303 27.083037576125836 29.451452087332385 21.70212530899148 19 23.715138402427296 26.75815243958686 27.199489176202725 22.327219981783124 20 24.076261075145275 26.89360127267505 27.303133078503723 21.727004573675952 21 23.61527179722306 26.428616804721962 26.716972376972432 23.23913902108255 22 23.5852559002917 27.24669627861752 26.67920754883762 22.48884027225316 23 22.6201668399289 27.031228077540465 27.43650703341549 22.912098049115148 24 23.484940564455357 26.83107664632812 27.258694340640034 22.425288448576485 25 23.146749191116395 26.611980592317693 27.33150997314678 22.909760243419136 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 235.0 1 235.0 2 321.5 3 408.0 4 408.0 5 408.0 6 847.5 7 1287.0 8 1287.0 9 1287.0 10 1966.0 11 2645.0 12 2645.0 13 2645.0 14 4858.5 15 7072.0 16 7072.0 17 7072.0 18 13099.0 19 19126.0 20 19126.0 21 19126.0 22 30536.5 23 41947.0 24 41947.0 25 41947.0 26 60750.5 27 79554.0 28 79554.0 29 79554.0 30 103132.0 31 126710.0 32 126710.0 33 126710.0 34 145715.0 35 164720.0 36 164720.0 37 164720.0 38 180953.0 39 197186.0 40 197186.0 41 197186.0 42 209526.5 43 221867.0 44 221867.0 45 221867.0 46 232275.5 47 242684.0 48 242684.0 49 242684.0 50 234696.5 51 226709.0 52 226709.0 53 226709.0 54 203655.0 55 180601.0 56 180601.0 57 180601.0 58 155711.0 59 130821.0 60 130821.0 61 130821.0 62 107264.0 63 83707.0 64 83707.0 65 83707.0 66 65246.5 67 46786.0 68 46786.0 69 46786.0 70 34437.0 71 22088.0 72 22088.0 73 22088.0 74 15631.0 75 9174.0 76 9174.0 77 9174.0 78 6392.0 79 3610.0 80 3610.0 81 3610.0 82 2495.5 83 1381.0 84 1381.0 85 1381.0 86 900.0 87 419.0 88 419.0 89 419.0 90 267.0 91 115.0 92 115.0 93 115.0 94 68.5 95 22.0 96 22.0 97 22.0 98 18.5 99 15.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16671369697424857 2 0.25898881709480814 3 0.2600380255222711 4 0.4606024996562462 5 0.2573321722093403 6 0.018885751694333555 7 0.018499201221057724 8 0.018002207755417367 9 0.018940973190515818 10 0.021481162014899864 11 0.020763282564530458 12 0.028328627541500336 13 0.02766596958731319 14 0.026230210686574384 15 0.04174745111378997 16 0.021149833037806293 17 0.04091912867105604 18 0.03114492384679569 19 0.026506318167485693 20 0.029212171480416525 21 0.0344582136177314 22 0.04467419041144984 23 0.029322614472781047 24 0.04754570821292746 25 0.03744017441157354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1810889.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.44102082698302 #Duplication Level Percentage of deduplicated Percentage of total 1 83.28916040325292 61.16840963837227 2 11.470062859022374 16.847462506325332 3 2.852977779259368 6.2857680151652096 4 1.0142968573307174 2.9796398649587457 5 0.4709315418119691 1.7292846585148025 6 0.2503536822450201 1.1031737995121045 7 0.15532835948756712 0.7985231298903266 8 0.10315561636991329 0.6060683016194458 9 0.06395607527700005 0.4227299510787229 >10 0.29959732214398743 3.7437657135282745 >50 0.016991150479373968 0.8695629586487632 >100 0.010901868498158052 1.688757832290785 >500 0.001448107053718935 0.7059431606321609 >1k 8.383777679425389E-4 1.0509104694631137 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2674 0.14766228079136823 No Hit CCCATGTACTCTGCGTTGATACCAC 2192 0.12104551963151801 No Hit GTCCTACAGTGGACATTTCTAAATT 2048 0.11309362418127229 No Hit GTATCAACGCAGAGTACTTTTTTTT 2048 0.11309362418127229 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1889 0.10431340628829265 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 420 0.0 12.479786 4 CGGGCCG 55 0.0030962825 12.076512 10 CGCAATT 55 0.0030962825 12.076512 11 TATACTG 205 0.0 11.597841 5 CGCAGAT 180 0.0 11.596325 8 GTCCTAT 185 0.0 11.357573 1 GGCGAGG 550 0.0 11.215144 19 GTCTACG 60 0.0056480463 11.142467 1 TATCGCA 60 0.0059322864 11.06922 8 GTATTAT 165 7.2759576E-11 10.997759 1 TGGCGAA 95 1.3768209E-5 10.988116 18 GTCCTAC 1290 0.0 10.88334 1 ATTCCGT 70 0.0015078938 10.843018 6 AGGACCT 815 0.0 10.618784 5 CTAATAC 190 9.094947E-12 10.53347 3 TGTAGGA 1275 0.0 10.471887 2 TTAGGAC 850 0.0 10.42725 3 GTAGGAC 1195 0.0 10.367671 3 GGACGTG 1040 0.0 10.217459 6 GTATTAA 215 0.0 10.217012 1 >>END_MODULE