FastQCFastQC Report
Thu 26 May 2016
SRR1513176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810889
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30140.16643758949333726No Hit
GTACATGGGAAGCAGTGGTATCAAC29810.1646152801193226No Hit
CCCATGTACTCTGCGTTGATACCAC27880.15395753135614607No Hit
GTCCTACAGTGGACATTTCTAAATT20970.11579947749420312No Hit
GTCCTAAAGTGTGTATTTCTCATTT20330.11226530173853837No Hit
TATCAACGCAGAGTACTTTTTTTTT19820.10944900543324301No Hit
CTGTAGGACGTGGAATATGGCAAGA19000.10492084274629754No Hit
CTTTAGGACGTGAAATATGGCGAGG18720.10337464085319421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5100.012.3181694
TAGGACT2500.012.1837894
AATCCCG1553.2014214E-1011.01935119
CTAGGAC2350.010.9368523
TAGAACA5150.010.5351334
ATAGGAC2100.010.4256943
GGCGAGG4850.010.36929819
GTCCTAA5800.010.1795511
AGGACCT10000.010.0897015
TAGCCCC856.476622E-410.0785024
ATTGGGC951.6111485E-410.0201183
TTAATCT2002.3646862E-119.9945144
TAAGAGT2002.3646862E-119.9945144
TGGCGAG11600.09.98244818
TCAATAC1451.5702244E-79.8473593
CTAAGAC1856.2573235E-109.7763863
GGACCTG9250.09.7753026
AGGACGT11700.09.7626515
AGGACTG4100.09.7507465
TTAGGAC7450.09.7107723
Produced by FastQC (version 0.11.5)