FastQCFastQC Report
Thu 26 May 2016
SRR1513175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489843
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT31010.12454600551119087No Hit
GTCCTACAGTGTGCATTTCTCATTT27660.11109134190388711No Hit
GTCCTAAAGTGTGTATTTCTCATTT26510.10647257678496194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7650.012.4578994
GTCCTAC17900.011.20679951
GCGTTTA600.00592768611.07063913
GGACCGG1053.5281537E-610.8416576
AGGACCT14050.010.6282745
GTAGGAC16250.010.6157083
TGGCGAG15650.010.54999218
TCCTACA20950.010.5159822
GGACCTG13650.010.4246716
GGACGTG12150.010.3843456
TGTAGGA18200.010.2708542
TATGGCG15700.010.15355416
ATGGCGA16200.010.01588917
GGCGAGA9600.09.98354419
CCTACAG20700.09.852973
TAGACTA1752.5229383E-99.7830415
AGGACGT12850.09.6963345
GGCGAGG7650.09.67537519
GTCCTAA9900.09.6489521
CTACAGT21950.09.5954194
Produced by FastQC (version 0.11.5)