##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513175_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2489843
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.632278822399645	31.0	30.0	34.0	25.0	34.0
2	29.498869205809363	31.0	30.0	34.0	23.0	34.0
3	29.679457700746593	31.0	30.0	34.0	25.0	34.0
4	32.7642650560698	37.0	33.0	37.0	25.0	37.0
5	32.9162513459684	37.0	35.0	37.0	26.0	37.0
6	33.303080555681625	37.0	35.0	37.0	28.0	37.0
7	33.28241338911731	37.0	35.0	37.0	27.0	37.0
8	33.17226467692943	37.0	35.0	37.0	27.0	37.0
9	34.44225519440382	39.0	35.0	39.0	26.0	39.0
10	34.01793607066791	39.0	34.0	39.0	23.0	39.0
11	34.48322082958644	39.0	35.0	39.0	27.0	39.0
12	34.47323626429458	39.0	35.0	39.0	27.0	39.0
13	34.51555861152691	39.0	35.0	39.0	27.0	39.0
14	34.76221874230624	40.0	34.0	41.0	17.0	41.0
15	35.19964351165917	40.0	35.0	41.0	25.0	41.0
16	35.04650855495708	40.0	35.0	41.0	23.0	41.0
17	34.661756986283876	39.0	34.0	41.0	17.0	41.0
18	34.40061562114559	39.0	33.0	41.0	17.0	41.0
19	35.0336045284783	40.0	34.0	41.0	24.0	41.0
20	35.24938158751375	40.0	36.0	41.0	25.0	41.0
21	35.29309398223101	40.0	36.0	41.0	25.0	41.0
22	34.98728634697047	40.0	35.0	41.0	22.0	41.0
23	34.529810915788666	40.0	34.0	41.0	18.0	41.0
24	34.78568809358662	40.0	35.0	41.0	19.0	41.0
25	34.43380446076319	39.0	34.0	41.0	17.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7179709672236747
1101	2	0.9019630100960043
1101	3	0.8601684337570177
1101	4	1.229803690407934
1101	5	1.058661314676435
1101	6	0.740221813449061
1101	7	0.7732513910253971
1101	8	0.770220656530924
1101	9	1.1793565182292411
1101	10	1.5049126681806086
1101	11	1.0073670841940157
1101	12	0.999602798227734
1101	13	0.8869501836730933
1101	14	1.8824553903668715
1101	15	1.4502367533504525
1101	16	1.4373903316679915
1101	17	1.8230667980772282
1101	18	1.8865242533341444
1101	19	1.5187843795997509
1101	20	1.1195432155504292
1101	21	1.0830401014281321
1101	22	1.279044082042624
1101	23	1.5946252740080737
1101	24	1.3564650532138387
1101	25	1.5626982557605444
1102	1	1.0043392020816313
1102	2	1.2447634518224682
1102	3	1.0866681051802196
1102	4	1.46944541090825
1102	5	1.3777508887891727
1102	6	1.0733048721403122
1102	7	1.0098714032449223
1102	8	1.017197815730583
1102	9	1.362552953086933
1102	10	1.5582159062074297
1102	11	1.262281305702274
1102	12	1.1245620092576232
1102	13	1.1554468216974882
1102	14	2.174767672329459
1102	15	1.6569061536513985
1102	16	1.7506673770750112
1102	17	2.1212092906636073
1102	18	2.4147643954090867
1102	19	1.732301212274301
1102	20	1.5328842081256298
1102	21	1.4715159332418182
1102	22	1.738665084011167
1102	23	2.023014967115735
1102	24	1.8648296233846793
1102	25	2.133606400534248
1103	1	0.8211638865003827
1103	2	1.1478614974743095
1103	3	0.8343361438517221
1103	4	1.3715321321427751
1103	5	1.2136490088115508
1103	6	0.8674290102420414
1103	7	0.9013030125509047
1103	8	0.9175421772562657
1103	9	1.2791111381629108
1103	10	1.524336648722425
1103	11	1.170296381353083
1103	12	1.1149952736322248
1103	13	1.107489831087797
1103	14	1.9848333741631166
1103	15	1.6239459940435026
1103	16	1.690557329892144
1103	17	2.0432811790417986
1103	18	2.200000900500548
1103	19	1.6563912560215002
1103	20	1.2859144982090385
1103	21	1.2451201081280843
1103	22	1.5010797192139478
1103	23	1.9213148754490348
1103	24	1.4639970210375282
1103	25	1.7414576783138074
1104	1	0.607872608191542
1104	2	0.7892455631036022
1104	3	0.6826546153113213
1104	4	1.0547461486394951
1104	5	0.9487403062196051
1104	6	0.7543007428043609
1104	7	0.6877318495687632
1104	8	0.674726512770718
1104	9	1.0087053177227574
1104	10	1.2012343567884116
1104	11	0.8139338923140826
1104	12	0.7171938159969073
1104	13	0.7465595690309925
1104	14	1.7404997144790784
1104	15	1.3241719481258656
1104	16	1.4028647667696958
1104	17	1.7062268139293693
1104	18	1.881643513678597
1104	19	1.273936723312076
1104	20	0.9707299600920365
1104	21	1.061320126182352
1104	22	1.3007264854538647
1104	23	1.6522727762080436
1104	24	1.4124475905161233
1104	25	1.6331384637847108
1105	1	0.8218002377943279
1105	2	1.0781400818226103
1105	3	0.8890114061513721
1105	4	1.3106442330363492
1105	5	1.0876813066689195
1105	6	0.8718947101919667
1105	7	0.8751732663760876
1105	8	0.9324081843599288
1105	9	1.0879125378729881
1105	10	1.4802081171479244
1105	11	0.9649921400330186
1105	12	0.9070137291336167
1105	13	0.8085141391524857
1105	14	1.686325712108136
1105	15	1.3717617572781933
1105	16	1.5655669284463016
1105	17	1.8457119125173165
1105	18	1.4184850150759019
1105	19	1.2802618018179217
1105	20	1.181897120081473
1105	21	1.1814528618985278
1105	22	1.3075243271622767
1105	23	1.628217367277493
1105	24	1.4684614071169193
1105	25	1.6872362852898775
1106	1	0.5262437162115532
1106	2	0.7492757173327824
1106	3	0.7203434060850924
1106	4	1.2603968560294234
1106	5	1.1353974103074265
1106	6	0.8340941355820561
1106	7	0.7740709729075945
1106	8	0.8018407209323399
1106	9	1.0087824277564579
1106	10	0.926846924153665
1106	11	0.8613145731241758
1106	12	0.9086476548813778
1106	13	0.6787068051725456
1106	14	1.658851474890632
1106	15	1.2366876005864569
1106	16	1.386419930481658
1106	17	1.700084719344666
1106	18	1.7505098398600722
1106	19	1.3635174122677967
1106	20	1.128912571933654
1106	21	0.9986889734062743
1106	22	1.2152810674056695
1106	23	1.6824626162040204
1106	24	1.1681822111477231
1106	25	1.5439267112902542
1107	1	0.532089868392589
1107	2	0.8138908827079199
1107	3	0.7078131321985204
1107	4	1.022267050262407
1107	5	0.96418909405309
1107	6	0.7738150011203473
1107	7	0.6942739754999039
1107	8	0.7243936181922024
1107	9	0.9132852616447877
1107	10	1.2321674825335975
1107	11	0.6756914881807887
1107	12	0.7849431496608261
1107	13	0.7074800061328759
1107	14	1.4840605926207004
1107	15	0.9610005529949532
1107	16	1.216364498776798
1107	17	1.5848059775727634
1107	18	1.73238331992156
1107	19	1.1599968995675383
1107	20	0.971488361760521
1107	21	0.8214847139290598
1107	22	1.0580762362386977
1107	23	1.4683594600602845
1107	24	1.212964251652778
1107	25	1.4899236064043322
1108	1	0.6368234708356688
1108	2	0.7518859621466092
1108	3	0.5628820033482356
1108	4	1.1028811644723504
1108	5	1.0161716304978157
1108	6	0.5647881103239953
1108	7	0.5792360646622043
1108	8	0.5539748774384492
1108	9	0.7767476286911545
1108	10	1.0763551174183021
1108	11	0.8041951351866672
1108	12	0.5068059836729617
1108	13	0.48812881134093544
1108	14	1.4177049853748471
1108	15	1.0189599252207344
1108	16	1.1175145334404064
1108	17	1.4892464527230231
1108	18	1.6890108755579476
1108	19	1.097126279049263
1108	20	0.7736006641772804
1108	21	0.7572978761292717
1108	22	0.9835360597350657
1108	23	1.365794832497528
1108	24	1.009193053919013
1108	25	1.277170617541337
1109	1	0.44089248639433265
1109	2	0.5653336927999888
1109	3	0.4738982637356628
1109	4	0.6892038963566449
1109	5	0.8231726579936094
1109	6	0.3991478153300463
1109	7	0.44501957775896983
1109	8	0.41039923522659905
1109	9	0.7072548786944992
1109	10	1.0524774748068921
1109	11	0.5700945771122505
1109	12	0.4813490569524177
1109	13	0.3933629966131136
1109	14	1.5067634568598436
1109	15	0.9001431951556285
1109	16	1.0893866842034612
1109	17	1.3726565705822011
1109	18	1.5289403982762337
1109	19	1.028836658240671
1109	20	0.7592164649232203
1109	21	0.7200278503143949
1109	22	0.9847994195748271
1109	23	1.2945352942352812
1109	24	1.0266202837582412
1109	25	1.311638130692593
1110	1	0.4261210047330515
1110	2	0.6117853726633911
1110	3	0.5993275956405135
1110	4	0.8896556766603112
1110	5	0.7353272020785155
1110	6	0.42814016513000297
1110	7	0.5332795841201872
1110	8	0.43878465799640765
1110	9	0.6698314392850833
1110	10	0.9746524752253976
1110	11	0.5698998569220777
1110	12	0.5657881817829278
1110	13	0.49511983073602295
1110	14	1.306201861868466
1110	15	0.9380105805281787
1110	16	1.1232902950309196
1110	17	1.3541884854758166
1110	18	0.9721988812934583
1110	19	0.7276416869498448
1110	20	0.6706982601255262
1110	21	0.399477303751965
1110	22	0.5834008223905016
1110	23	-0.5360211783026756
1110	24	0.1512983894548725
1110	25	0.6160999550225696
1111	1	0.25918642375956225
1111	2	0.6181995563619864
1111	3	0.5908610438880721
1111	4	0.9751853491547706
1111	5	0.7749903392368509
1111	6	0.4333323181300841
1111	7	0.40510163123501286
1111	8	0.5012208395608795
1111	9	0.7529206619724391
1111	10	1.0663940823829066
1111	11	0.6560106670673633
1111	12	0.47568229653298744
1111	13	0.41466903864868954
1111	14	1.3149133450827861
1111	15	0.9569339984055532
1111	16	0.9763806668605213
1111	17	1.3277050249160212
1111	18	1.5452935213656929
1111	19	1.0075362941894426
1111	20	0.6794077733816977
1111	21	0.47954395400231675
1111	22	0.8518430779925694
1111	23	1.1480687831189726
1111	24	0.7753020010447997
1111	25	0.9913668022687361
1112	1	0.2549739727545628
1112	2	0.3882828194823986
1112	3	0.27557661507100306
1112	4	0.6885216531595333
1112	5	0.4069377700276675
1112	6	0.29809334447760705
1112	7	0.19354195738620206
1112	8	0.3283865920001716
1112	9	0.5504374271317758
1112	10	0.8209071355001996
1112	11	0.1722447378147507
1112	12	0.20392423631656698
1112	13	0.16172823608742704
1112	14	0.7449539031798409
1112	15	0.4997546703869915
1112	16	0.4251231582938928
1112	17	0.9612014857307258
1112	18	1.0810838853637463
1112	19	0.650044851085255
1112	20	0.47364110340708265
1112	21	0.3927976889081606
1112	22	0.6382843628014783
1112	23	1.0344083645915845
1112	24	0.6206240043151325
1112	25	0.9376226211849072
1113	1	0.12130372309437831
1113	2	0.2124660319111733
1113	3	0.24519115554538828
1113	4	0.48421172846545346
1113	5	0.49862622513893484
1113	6	0.21572297298197896
1113	7	0.10217336369145613
1113	8	0.167872930578298
1113	9	0.30025308939322315
1113	10	0.5608832450935637
1113	11	0.1685383395584097
1113	12	0.0838084497577043
1113	13	-0.04141002019575524
1113	14	0.5182386193239523
1113	15	0.2841500036449389
1113	16	0.4290400692327552
1113	17	0.7985737924370326
1113	18	0.6042457478778189
1113	19	0.37125724175044184
1113	20	0.14275818571570653
1113	21	0.08321695225258452
1113	22	0.19805932441647656
1113	23	0.45888587571486994
1113	24	0.30553333555333495
1113	25	0.7362194084667166
1114	1	-1.0484315272546958
1114	2	-0.632482193974333
1114	3	-0.6692823750339727
1114	4	-0.4535074813206492
1114	5	-3.523857450356811
1114	6	-1.342502407651338
1114	7	-1.0270398182291345
1114	8	-0.7071797254305849
1114	9	-0.4268394104994755
1114	10	-0.2660312843381618
1114	11	-0.7435210051497094
1114	12	-0.777460888483624
1114	13	-0.8203924810646299
1114	14	-6.3250559241371995
1114	15	-2.6824001077203192
1114	16	-1.4005813280297446
1114	17	-0.4450822103088399
1114	18	-6.431316969228337
1114	19	-2.5667758493793897
1114	20	-1.5417058749356656
1114	21	-1.180056830162414
1114	22	-1.0367034388195435
1114	23	-0.6015097347993432
1114	24	-0.7050936222097945
1114	25	-0.48314138248295535
1115	1	-0.4320937886747451
1115	2	-0.12262812417328917
1115	3	-0.09802989869675116
1115	4	-2.6317902344542716
1115	5	-0.6026282771060494
1115	6	-0.40321217416547483
1115	7	-0.26737432511983883
1115	8	-0.05762555835913474
1115	9	0.14508067774482214
1115	10	-0.06369728654919271
1115	11	-0.2064331900200429
1115	12	-0.29476481360877926
1115	13	-0.3148240886578719
1115	14	-5.853119234749514
1115	15	-2.171986763844231
1115	16	-0.8754131338529305
1115	17	-0.057017325662194196
1115	18	0.284687516769516
1115	19	-0.10385124439437021
1115	20	-0.1619090975250259
1115	21	-0.24411154261216694
1115	22	-1.2932912029160661
1115	23	-0.4746927396554881
1115	24	-0.12274863077475828
1115	25	0.23220680497961155
1116	1	-0.10696013949295491
1116	2	0.08390579882768989
1116	3	-0.003299835913686877
1116	4	0.18028233928517068
1116	5	-0.37772865620188867
1116	6	-0.201456989831442
1116	7	-0.1645672067088526
1116	8	-0.13538404805817095
1116	9	-0.048953238319107584
1116	10	0.12964059378838044
1116	11	-0.07339080576192458
1116	12	-0.45473010616632337
1116	13	-0.41922079017442115
1116	14	0.46483429146337585
1116	15	0.08416559372567178
1116	16	0.1098251593222912
1116	17	0.47536015634448603
1116	18	0.5358595172867453
1116	19	0.11716129834478295
1116	20	-0.17556076555228373
1116	21	-0.3708432658472276
1116	22	-0.34551674690555245
1116	23	-0.9078791925426586
1116	24	-0.6259562666340841
1116	25	6.342426169325677E-4
1201	1	0.7633242113278769
1201	2	0.9530532700383851
1201	3	0.9660968640269125
1201	4	1.3586923359334122
1201	5	1.2384301239919964
1201	6	0.9793517899095008
1201	7	0.8323632245484305
1201	8	0.9873069347485952
1201	9	1.129181457623453
1201	10	1.5512850616340401
1201	11	1.0926630474490011
1201	12	1.1447123307952722
1201	13	0.9364156024320565
1201	14	1.8797130133433981
1201	15	1.5649693104651305
1201	16	1.635571762799053
1201	17	2.0031153939760884
1201	18	1.4754010749117086
1201	19	1.326679778362248
1201	20	1.2764553203976305
1201	21	1.15874879894033
1201	22	1.4063469774457786
1201	23	1.619721735227543
1201	24	1.305226188675931
1201	25	1.69455855397117
1202	1	0.7553305454656929
1202	2	0.9317075295797395
1202	3	0.8742461303589373
1202	4	1.2681416662721219
1202	5	1.072356589722041
1202	6	0.8443665183284708
1202	7	0.6890345731852463
1202	8	0.8604283157328751
1202	9	1.1547961741128603
1202	10	1.4594035003757497
1202	11	0.9349030690598639
1202	12	0.9056740483766887
1202	13	0.7591317224801415
1202	14	1.6563011647662265
1202	15	1.2990105121848217
1202	16	1.4070399533141398
1202	17	1.8032202651368365
1202	18	2.029648170522549
1202	19	1.5759535376879512
1202	20	1.2559707450899822
1202	21	1.1816829489132559
1202	22	1.3202227196020928
1202	23	1.6352043547755102
1202	24	1.385037386174396
1202	25	1.853272180282275
1203	1	0.6062970706629471
1203	2	0.7156985465741847
1203	3	0.5287157786028764
1203	4	0.9368423803378363
1203	5	0.9515726461225853
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2211	15	-0.3471812398945602
2211	16	-6.94886327268317
2211	17	-2.252712758494731
2211	18	-0.9278922176427358
2211	19	-0.9243553976221364
2211	20	-1.0674096270899938
2211	21	-0.9706518759153795
2211	22	-0.7965405742305478
2211	23	-0.4022156111454649
2211	24	-0.8668400933760338
2211	25	-8.239165029540398
2212	1	-1.7168777825577557
2212	2	-4.316859465237812
2212	3	-1.94706774755619
2212	4	-3.88783288344964
2212	5	-1.5057037624295262
2212	6	-1.2262613224599406
2212	7	-1.0744216043348231
2212	8	-0.9655988814974918
2212	9	-1.0955038343829528
2212	10	-0.5243786509772121
2212	11	-1.0738559842938642
2212	12	-1.1323587985711896
2212	13	-1.236093363539993
2212	14	-6.251751408403987
2212	15	-5.1156807445587305
2212	16	-2.9207553028347064
2212	17	-7.795044597230948
2212	18	-3.3271853858259526
2212	19	-2.1164760266890923
2212	20	-2.1406653100903412
2212	21	-1.5998568380541656
2212	22	-1.5191397225201584
2212	23	-0.9944879904944486
2212	24	-1.3916114658284968
2212	25	-1.1404876430047537
2213	1	-0.5322524260630708
2213	2	-0.4328477117160787
2213	3	-0.4765595805870433
2213	4	-0.13826931936701925
2213	5	-0.40881930991893967
2213	6	-0.6202030733889856
2213	7	-0.7305662763597169
2213	8	-0.6234892971918953
2213	9	-0.4608223029682108
2213	10	-4.241421257493499
2213	11	-1.7947357699649373
2213	12	-1.2393460943969572
2213	13	-1.138452710182456
2213	14	-0.35688061072012545
2213	15	-0.8414866386041879
2213	16	-0.6210923523550633
2213	17	-0.2168361450660541
2213	18	-0.08186086763392098
2213	19	-0.6755720425909146
2213	20	-1.0794260049308875
2213	21	-0.9526612629094018
2213	22	-1.066460610948127
2213	23	-1.507572425720447
2213	24	-0.9993203933196355
2213	25	-0.6839324619486646
2214	1	-1.0056709116254865
2214	2	-0.7729750506440176
2214	3	-0.7461104382750818
2214	4	-0.28329721126522855
2214	5	-3.662132507216395
2214	6	-1.4962620406585998
2214	7	-1.1927620453583287
2214	8	-0.9023861312424017
2214	9	-0.8305659214187244
2214	10	-4.306899800689436
2214	11	-1.8699273138034087
2214	12	-1.427775824337182
2214	13	-1.1965379532751967
2214	14	-0.3860957920904795
2214	15	-0.6554773705109653
2214	16	-0.7827660609553106
2214	17	-0.4221672457494776
2214	18	-0.1707165209760788
2214	19	-0.8893159823093697
2214	20	-1.175890353925226
2214	21	-1.1605230787547285
2214	22	-0.8187766607154501
2214	23	-0.5817096266005208
2214	24	-1.003297300969706
2214	25	-0.6705144144788946
2215	1	-2.229546010825427
2215	2	-4.704324835176443
2215	3	-2.996756925367759
2215	4	-1.8791239312692412
2215	5	-1.8238989821206175
2215	6	-2.157240942772006
2215	7	-2.2108913466339715
2215	8	-2.3987596683144936
2215	9	-8.089732514157532
2215	10	-3.6906318663528026
2215	11	-3.1316545426561007
2215	12	-3.293876095106011
2215	13	-2.7788114664332326
2215	14	-7.800072870757113
2215	15	-4.835177278036852
2215	16	-9.528147899965496
2215	17	-4.645944229820593
2215	18	-3.032828182742616
2215	19	-3.5261416276272755
2215	20	-3.2739039323840586
2215	21	-3.350287501196526
2215	22	-9.442596265805673
2215	23	-10.624162078128755
2215	24	-6.664648474060222
2215	25	-5.063361062220029
2216	1	-1.8712643281618035
2216	2	-1.8820699892308355
2216	3	-1.7703439640473704
2216	4	-6.811603733187518
2216	5	-3.4474077675767774
2216	6	-2.638943514501676
2216	7	-2.172875338299022
2216	8	-7.8340731254948786
2216	9	-3.8956988306781426
2216	10	-2.2254607120148826
2216	11	-2.1313882533353237
2216	12	-2.300292418533978
2216	13	-2.1838450411585058
2216	14	-1.546961282022231
2216	15	-2.0842880831090014
2216	16	-1.7210204466952845
2216	17	-1.5247957591951717
2216	18	-7.594603235361749
2216	19	-4.694256785351271
2216	20	-3.9764593828533386
2216	21	-3.165267352291565
2216	22	-2.75297407670962
2216	23	-2.021947394333054
2216	24	-2.4875575404682877
2216	25	-1.9310934438832277
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102360.0
3	1.0
4	877.0
5	5149.0
6	6224.0
7	5684.0
8	5198.0
9	4876.0
10	4537.0
11	4482.0
12	4379.0
13	4419.0
14	4399.0
15	4406.0
16	4458.0
17	5025.0
18	5565.0
19	6722.0
20	8664.0
21	11248.0
22	14092.0
23	16694.0
24	19513.0
25	22221.0
26	25382.0
27	28291.0
28	31756.0
29	38339.0
30	45896.0
31	55072.0
32	70981.0
33	92911.0
34	123095.0
35	165699.0
36	237345.0
37	349657.0
38	954226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69353712268234	18.41080032055305	12.156596280168516	25.73906627659609
2	14.419590348337197	20.116194275088418	41.47599265047855	23.988222726095835
3	18.42841869371138	24.809295713960687	29.662484722918542	27.099800869409396
4	11.243398091549153	16.7051181181787	39.29540640319554	32.7560773870766
5	12.63361732593809	38.008003549974674	36.4420040605654	12.916375063521837
6	31.867073302021552	37.259437319934506	18.261232150116577	12.612257227927365
7	28.042767584626098	31.433114187490613	22.565340034916577	17.95877819296671
8	25.10310729867514	36.532742668204385	20.887508099350967	17.476641933769503
9	26.60855536822502	14.754428947830386	20.06551078770817	38.571504896236426
10	13.649554575722128	28.76762736049157	35.96085986810814	21.62195819567816
11	34.55200976965782	22.254011103344663	24.630785670097293	18.563193456900223
12	23.05827612209225	24.67343492823101	31.98270746800034	20.285581481676402
13	28.95748164860452	21.64845989689794	25.733212169128517	23.66084628536902
14	20.323906612309962	21.49253827278731	28.78377140616639	29.399783708736337
15	23.169625798913472	30.337718358968342	23.417692274621725	23.07496356749646
16	22.26204593733831	27.769730012887134	27.774269434347914	22.19395461542664
17	21.574388068337054	28.1250301345205	28.26698381575352	22.03359798138892
18	21.4197815813357	27.30426146601566	29.71041829628942	21.565538656359216
19	23.46044048134611	26.93511051403953	27.40221100391855	22.20223800069581
20	23.64124157486414	27.102412128901314	27.715518159163306	21.54082813707124
21	23.261277968929036	26.69488937779328	26.89922748464049	23.144605168637195
22	23.19475723903221	27.70912480688213	27.02034945043023	22.07576850365543
23	22.297674612937673	27.363814081523934	27.79798114524971	22.54053016028868
24	23.152602074458873	26.904075263325606	27.703263899408874	22.240058762806648
25	22.772050879552193	27.01948663224501	27.709982213290253	22.498480274912545
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	219.0
1	219.0
2	360.5
3	502.0
4	502.0
5	502.0
6	949.0
7	1396.0
8	1396.0
9	1396.0
10	2381.5
11	3367.0
12	3367.0
13	3367.0
14	6548.5
15	9730.0
16	9730.0
17	9730.0
18	18624.5
19	27519.0
20	27519.0
21	27519.0
22	44282.0
23	61045.0
24	61045.0
25	61045.0
26	87932.5
27	114820.0
28	114820.0
29	114820.0
30	147605.0
31	180390.0
32	180390.0
33	180390.0
34	208824.0
35	237258.0
36	237258.0
37	237258.0
38	259436.0
39	281614.0
40	281614.0
41	281614.0
42	296754.5
43	311895.0
44	311895.0
45	311895.0
46	320745.5
47	329596.0
48	329596.0
49	329596.0
50	317958.0
51	306320.0
52	306320.0
53	306320.0
54	273906.5
55	241493.0
56	241493.0
57	241493.0
58	207167.0
59	172841.0
60	172841.0
61	172841.0
62	140215.0
63	107589.0
64	107589.0
65	107589.0
66	83062.5
67	58536.0
68	58536.0
69	58536.0
70	42709.5
71	26883.0
72	26883.0
73	26883.0
74	18794.5
75	10706.0
76	10706.0
77	10706.0
78	7419.5
79	4133.0
80	4133.0
81	4133.0
82	2766.0
83	1399.0
84	1399.0
85	1399.0
86	917.5
87	436.0
88	436.0
89	436.0
90	276.0
91	116.0
92	116.0
93	116.0
94	72.0
95	28.0
96	28.0
97	28.0
98	20.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1662353811063589
2	0.25768693046107727
3	0.26298846955410443
4	0.45757905217316913
5	0.2588516625345454
6	0.018916855400119604
7	0.01931848714959136
8	0.017912776026440223
9	0.019358650324538533
10	0.021326645896950127
11	0.02020207699842922
12	0.02715030626429056
13	0.02646753229018858
14	0.02437904719293546
15	0.03992219589749233
16	0.02164795129652753
17	0.040283664472016915
18	0.030925644709325045
19	0.02638720594029423
20	0.028676506912283226
21	0.033375598381102746
22	0.04329590259305506
23	0.028475691037547345
24	0.0479548308869274
25	0.0363878365021409
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2489843.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.52482248874884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.96633781575802	58.626277623249145
2	12.094018094046074	17.30044994704724
3	3.1556509862084345	6.771221298750106
4	1.147320023554579	3.282474440901136
5	0.5548479242817707	1.98426996462522
6	0.30048292998163334	1.2895192936701283
7	0.1748760737655663	0.8755586093528034
8	0.11315977132168259	0.6474986045320607
9	0.09029440934574962	0.581246244016305
>10	0.37205476372800933	4.544533767123757
>50	0.018272903090503053	0.8936666109797389
>100	0.010640814702348629	1.5520784898515652
>500	0.0014190904275037629	0.6960477092669421
>1k	6.243997880852911E-4	0.9551573966338427
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3101	0.12454600551119087	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2766	0.11109134190388711	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2651	0.10647257678496194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.032634989435077E-5	0.0
2	0.0	0.0	0.0	8.032634989435077E-5	0.0
3	0.0	0.0	0.0	8.032634989435077E-5	0.0
4	0.0	0.0	0.0	8.032634989435077E-5	0.0
5	0.0	0.0	0.0	8.032634989435077E-5	0.0
6	2.0081587473587693E-4	0.0	0.0	8.032634989435077E-5	0.0
7	2.0081587473587693E-4	0.0	0.0	8.032634989435077E-5	0.0
8	2.0081587473587693E-4	0.0	0.0	1.2048952484152615E-4	0.0
9	2.0081587473587693E-4	0.0	0.0	1.2048952484152615E-4	4.016317494717539E-5
10	2.0081587473587693E-4	0.0	0.0	1.2048952484152615E-4	4.016317494717539E-5
11	2.409790496830523E-4	0.0	0.0	1.2048952484152615E-4	4.016317494717539E-5
12	2.409790496830523E-4	0.0	0.0	1.2048952484152615E-4	8.032634989435077E-5
13	2.409790496830523E-4	0.0	0.0	1.2048952484152615E-4	8.032634989435077E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	765	0.0	12.457899	4
GTCCTAC	1790	0.0	11.2067995	1
GCGTTTA	60	0.005927686	11.070639	13
GGACCGG	105	3.5281537E-6	10.841657	6
AGGACCT	1405	0.0	10.628274	5
GTAGGAC	1625	0.0	10.615708	3
TGGCGAG	1565	0.0	10.549992	18
TCCTACA	2095	0.0	10.515982	2
GGACCTG	1365	0.0	10.424671	6
GGACGTG	1215	0.0	10.384345	6
TGTAGGA	1820	0.0	10.270854	2
TATGGCG	1570	0.0	10.153554	16
ATGGCGA	1620	0.0	10.015889	17
GGCGAGA	960	0.0	9.983544	19
CCTACAG	2070	0.0	9.85297	3
TAGACTA	175	2.5229383E-9	9.783041	5
AGGACGT	1285	0.0	9.696334	5
GGCGAGG	765	0.0	9.675375	19
GTCCTAA	990	0.0	9.648952	1
CTACAGT	2195	0.0	9.595419	4
>>END_MODULE