FastQCFastQC Report
Thu 26 May 2016
SRR1513175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489843
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30870.12398372106193041No Hit
GTCCTACAGTGGACATTTCTAAATT29950.12028870896679028No Hit
GTCCTACAGTGTGCATTTCTCATTT26830.10775779838327156No Hit
GTACATGGGAAGCAGTGGTATCAAC26410.1060709450354902No Hit
GTCCTAAAGTGTGTATTTCTCATTT25700.10321935961424074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG456.8079395E-414.76526318
TAAGCCG500.001477669513.3264855
TAGGACC7900.012.6517264
GGCGAGG7550.011.93944119
CGTGCGC1157.199742E-811.55124610
AAGGCGT1657.2759576E-1211.5378496
TTAGGAC11600.011.2430763
GGCGTGC1701.2732926E-1111.1643138
TGGCGAG16650.010.83165918
TAGTACC1951.8189894E-1210.7392914
TGTAGGA20100.010.6092172
CGGGAGT909.329336E-510.5765764
GGACCTG13050.010.5763636
AGGACCT13600.010.5687985
AGGACGT15650.010.522515
AGGCGTG1909.094947E-1210.4911077
GTCCTAC17850.010.4563571
TAGGACG16400.010.389554
GCACCGT1105.9082868E-610.3840656
TAAGACA3600.010.3121614
Produced by FastQC (version 0.11.5)