##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513175_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2489843 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.830460394490736 31.0 31.0 34.0 27.0 34.0 2 30.941963408937834 33.0 31.0 34.0 27.0 34.0 3 31.041706645760396 33.0 31.0 34.0 27.0 34.0 4 34.427366303819156 37.0 35.0 37.0 31.0 37.0 5 34.17975269926658 37.0 35.0 37.0 30.0 37.0 6 34.0981921350061 37.0 35.0 37.0 30.0 37.0 7 33.96565727236617 37.0 35.0 37.0 30.0 37.0 8 33.94013518121424 37.0 35.0 37.0 30.0 37.0 9 35.506583346821465 39.0 37.0 39.0 30.0 39.0 10 35.34991724377802 39.0 35.0 39.0 29.0 39.0 11 35.364642670240656 39.0 35.0 39.0 29.0 39.0 12 35.30579277488581 39.0 35.0 39.0 29.0 39.0 13 35.273086294999324 39.0 35.0 39.0 29.0 39.0 14 36.43482139235285 40.0 37.0 41.0 29.0 41.0 15 36.421514529229356 40.0 37.0 41.0 29.0 41.0 16 36.38840762248865 40.0 37.0 41.0 29.0 41.0 17 36.322171719261014 40.0 37.0 41.0 28.0 41.0 18 36.27491934230391 40.0 36.0 41.0 28.0 41.0 19 36.35686065346289 40.0 37.0 41.0 28.0 41.0 20 36.321868085658416 40.0 37.0 41.0 28.0 41.0 21 36.270904631336194 40.0 37.0 41.0 28.0 41.0 22 36.21065986891543 40.0 37.0 41.0 28.0 41.0 23 36.130868492511375 40.0 37.0 41.0 27.0 41.0 24 36.07037552166944 40.0 37.0 41.0 27.0 41.0 25 36.011980273454995 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32249790953224533 1101 2 0.38056042313330707 1101 3 0.38204473208378076 1101 4 0.4396863985205286 1101 5 0.3706791878249476 1101 6 0.34853636912534824 1101 7 0.4086822301485782 1101 8 0.4531728131013466 1101 9 0.4713587901295142 1101 10 0.5548347148997621 1101 11 0.55136587320942 1101 12 0.6071686179060123 1101 13 0.4871494185966654 1101 14 0.6059319073826543 1101 15 0.7142580122874875 1101 16 0.6178142205661032 1101 17 0.5247387079944659 1101 18 0.580561703790643 1101 19 0.5371423910257604 1101 20 0.5416860590923847 1101 21 0.49375011168528715 1101 22 0.46057843308958013 1101 23 0.44312097802250605 1101 24 0.5372369602466733 1101 25 0.45989587620339023 1102 1 0.5809480031952639 1102 2 0.5805023650667636 1102 3 0.5275963633576275 1102 4 0.6943071148018802 1102 5 0.6706863190447976 1102 6 0.709141333301929 1102 7 0.6483030538366208 1102 8 0.6276592227937243 1102 9 0.6042945189876718 1102 10 0.6986502302421869 1102 11 0.7683886210700877 1102 12 0.7693415444344254 1102 13 0.7796808248250002 1102 14 0.8125856716072803 1102 15 0.7003567367197618 1102 16 0.7923381860679513 1102 17 0.8604297765361579 1102 18 0.9022005428689681 1102 19 0.8736019654117655 1102 20 0.8522426464178992 1102 21 0.8867922623092213 1102 22 0.8091103735748746 1102 23 0.9071108750713606 1102 24 0.8988001820908877 1102 25 0.8799475220173321 1103 1 0.5239052251079386 1103 2 0.5913425193826889 1103 3 0.577010415441169 1103 4 0.6426545802073491 1103 5 0.6279681937009727 1103 6 0.557372322185536 1103 7 0.6187785538414801 1103 8 0.5513892736479704 1103 9 0.7055596284499686 1103 10 0.6405456683285635 1103 11 0.7138489979849325 1103 12 0.6619614175743109 1103 13 0.7498028143135329 1103 14 0.7582612928915182 1103 15 0.7056664661483083 1103 16 0.7868076875172036 1103 17 0.8590008544699401 1103 18 0.7733497533302298 1103 19 0.741512934093933 1103 20 0.8209392496820129 1103 21 0.7272588372600808 1103 22 0.8351239299923208 1103 23 0.7704748248659428 1103 24 0.8871674835688381 1103 25 0.9146092318266668 1104 1 0.3820227078787397 1104 2 0.49875069007039485 1104 3 0.5157071092753434 1104 4 0.596702190063354 1104 5 0.6025359144717086 1104 6 0.5072924131883312 1104 7 0.5854512337593576 1104 8 0.6115152790776008 1104 9 0.4633482158893685 1104 10 0.6352545635961135 1104 11 0.5691877346332674 1104 12 0.5778826684621521 1104 13 0.6125034539628516 1104 14 0.6563896575668053 1104 15 0.7182977042675418 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-1.399061396895931 2215 25 -1.3892488254221789 2216 1 -0.6441605452295143 2216 2 -0.5904923614543094 2216 3 -0.6924601351615465 2216 4 -0.5377552564381176 2216 5 -0.6862631465581401 2216 6 -0.8733384413450551 2216 7 -1.041189478677822 2216 8 -1.0552874121237181 2216 9 -1.1938410949873557 2216 10 -1.1025304866394308 2216 11 -1.064610701057866 2216 12 -1.086155704887517 2216 13 -1.1829851279934047 2216 14 -1.3854791752304436 2216 15 -1.2663758849522608 2216 16 -1.2507866963937389 2216 17 -1.3698662960379693 2216 18 -1.3542434986307228 2216 19 -1.3672138950412531 2216 20 -1.3831968147501996 2216 21 -1.6193884683451998 2216 22 -1.6154543246418527 2216 23 -1.518722986427882 2216 24 -1.545485534341239 2216 25 -1.6453184244405463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56292.0 3 1.0 4 887.0 5 7896.0 6 8825.0 7 6752.0 8 5679.0 9 4803.0 10 4484.0 11 3980.0 12 3586.0 13 3548.0 14 3467.0 15 3513.0 16 3585.0 17 3723.0 18 4055.0 19 4988.0 20 6536.0 21 8267.0 22 10603.0 23 12823.0 24 15007.0 25 16719.0 26 18333.0 27 20187.0 28 22803.0 29 27859.0 30 35338.0 31 45191.0 32 57582.0 33 75507.0 34 99968.0 35 134501.0 36 194552.0 37 317115.0 38 1240888.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.992443969499064 19.165400622507477 13.158678249897804 27.683477158095656 2 13.778409376183662 20.452822852579924 39.8988683847018 25.869899386534613 3 16.89570381628615 24.804414029332072 29.991268017245464 28.308614137136313 4 11.127087418336748 17.16621187742941 37.83110115760395 33.875599546629886 5 12.626078302594356 38.21626583942204 36.22566157195236 12.931994286031243 6 31.96759983932073 37.653784524588 17.840797787879996 12.537817848211274 7 27.777954099061404 31.462836807688426 22.60593304603231 18.153276047217858 8 25.093903757234838 36.94973763987151 20.475899541959343 17.480459060934308 9 26.37177525273825 14.565715961168479 19.70743815594462 39.35507063014865 10 14.086911258041946 29.15576533944263 35.144018012995645 21.613305389519784 11 34.48663228966646 22.171317629264177 24.566046935489506 18.77600314557986 12 22.79344520919592 24.795820459362297 31.933258442399783 20.477475889041997 13 28.85503222492342 21.546258137561285 25.82427888023462 23.77443075728068 14 20.824514038443155 21.561238104494887 27.759793333295846 29.854454523766112 15 23.009764069461408 30.537186481235967 23.27267221266562 23.18037723663701 16 22.43699703153974 27.932082464637332 27.218141866776342 22.412778637046593 17 21.815483064958332 28.35429918874894 27.573618171702424 22.256599574590307 18 21.759876581649156 27.441339121246877 29.059597966329125 21.73918633077484 19 23.401957472820577 26.976439879944238 27.33172332552695 22.289879321708238 20 23.546464576280513 27.05716786158806 27.711988265926806 21.68437929620462 21 23.09650123723654 26.641693003880196 27.01605696469187 23.245748794191396 22 23.119897760416542 27.735579583892605 26.892994644631496 22.251528011059353 23 22.356414288382407 27.402614355458354 27.392894367427594 22.84807698873164 24 22.941245693001527 26.904828939013427 27.777494404265653 22.3764309637194 25 22.721688206898687 27.071410698772652 27.524733546814513 22.682167547514155 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 118.0 1 118.0 2 263.5 3 409.0 4 409.0 5 409.0 6 935.0 7 1461.0 8 1461.0 9 1461.0 10 2355.5 11 3250.0 12 3250.0 13 3250.0 14 6391.5 15 9533.0 16 9533.0 17 9533.0 18 18110.5 19 26688.0 20 26688.0 21 26688.0 22 43233.0 23 59778.0 24 59778.0 25 59778.0 26 86154.5 27 112531.0 28 112531.0 29 112531.0 30 145166.0 31 177801.0 32 177801.0 33 177801.0 34 205559.5 35 233318.0 36 233318.0 37 233318.0 38 255199.0 39 277080.0 40 277080.0 41 277080.0 42 292679.0 43 308278.0 44 308278.0 45 308278.0 46 320036.0 47 331794.0 48 331794.0 49 331794.0 50 321344.0 51 310894.0 52 310894.0 53 310894.0 54 278145.0 55 245396.0 56 245396.0 57 245396.0 58 210556.0 59 175716.0 60 175716.0 61 175716.0 62 143115.0 63 110514.0 64 110514.0 65 110514.0 66 85611.0 67 60708.0 68 60708.0 69 60708.0 70 44099.5 71 27491.0 72 27491.0 73 27491.0 74 19262.0 75 11033.0 76 11033.0 77 11033.0 78 7585.5 79 4138.0 80 4138.0 81 4138.0 82 2745.5 83 1353.0 84 1353.0 85 1353.0 86 905.0 87 457.0 88 457.0 89 457.0 90 273.0 91 89.0 92 89.0 93 89.0 94 51.0 95 13.0 96 13.0 97 13.0 98 7.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17715976469199063 2 0.008675245788589882 3 0.00582366036734043 4 0.0018073428726228923 5 0.0765108482743691 6 0.31648581858374203 7 0.028114222463022767 8 0.013093195032779175 9 0.004538438769030819 10 0.003413869870509908 11 0.0 12 0.0 13 0.0 14 0.0014860374730454892 15 0.0 16 0.0 17 0.019197997624749833 18 0.03004205486048719 19 0.0 20 0.0 21 8.032634989435077E-4 22 2.0081587473587693E-4 23 0.0051408863932384495 24 0.0 25 2.409790496830523E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2489843.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.86215982297901 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2469767799091 55.25980140328986 2 12.964481800861133 17.85524435586004 3 3.5884048887580864 7.413159328776557 4 1.3284688432659038 3.6592493521929894 5 0.6093013853573401 2.097890468941984 6 0.35876379885512255 1.4823150033276327 7 0.21077174735097776 1.015993842657611 8 0.14012920611915786 0.7719679830115694 9 0.09830708119812914 0.609267414347653 >10 0.41682914747982147 4.967928489430534 >50 0.022359953425611524 1.0615902136564197 >100 0.012520329307300297 1.6743266239189907 >500 0.0017511118123695697 0.8157196400692707 >1k 9.339262999039563E-4 1.3155458805189484 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3087 0.12398372106193041 No Hit GTCCTACAGTGGACATTTCTAAATT 2995 0.12028870896679028 No Hit GTCCTACAGTGTGCATTTCTCATTT 2683 0.10775779838327156 No Hit GTACATGGGAAGCAGTGGTATCAAC 2641 0.1060709450354902 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2570 0.10321935961424074 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.016317494717539E-5 0.0 0.0 0.0 4.016317494717539E-5 2 4.016317494717539E-5 0.0 0.0 0.0 4.016317494717539E-5 3 4.016317494717539E-5 0.0 0.0 0.0 4.016317494717539E-5 4 4.016317494717539E-5 0.0 0.0 0.0 4.016317494717539E-5 5 4.016317494717539E-5 0.0 0.0 0.0 4.016317494717539E-5 6 2.0081587473587693E-4 0.0 0.0 0.0 4.016317494717539E-5 7 2.0081587473587693E-4 0.0 0.0 0.0 4.016317494717539E-5 8 2.409790496830523E-4 0.0 0.0 0.0 4.016317494717539E-5 9 2.409790496830523E-4 0.0 0.0 4.016317494717539E-5 4.016317494717539E-5 10 2.409790496830523E-4 0.0 0.0 4.016317494717539E-5 4.016317494717539E-5 11 2.409790496830523E-4 0.0 0.0 4.016317494717539E-5 4.016317494717539E-5 12 2.409790496830523E-4 0.0 0.0 4.016317494717539E-5 8.032634989435077E-5 13 2.409790496830523E-4 0.0 0.0 4.016317494717539E-5 8.032634989435077E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCGCG 45 6.8079395E-4 14.765263 18 TAAGCCG 50 0.0014776695 13.326485 5 TAGGACC 790 0.0 12.651726 4 GGCGAGG 755 0.0 11.939441 19 CGTGCGC 115 7.199742E-8 11.551246 10 AAGGCGT 165 7.2759576E-12 11.537849 6 TTAGGAC 1160 0.0 11.243076 3 GGCGTGC 170 1.2732926E-11 11.164313 8 TGGCGAG 1665 0.0 10.831659 18 TAGTACC 195 1.8189894E-12 10.739291 4 TGTAGGA 2010 0.0 10.609217 2 CGGGAGT 90 9.329336E-5 10.576576 4 GGACCTG 1305 0.0 10.576363 6 AGGACCT 1360 0.0 10.568798 5 AGGACGT 1565 0.0 10.52251 5 AGGCGTG 190 9.094947E-12 10.491107 7 GTCCTAC 1785 0.0 10.456357 1 TAGGACG 1640 0.0 10.38955 4 GCACCGT 110 5.9082868E-6 10.384065 6 TAAGACA 360 0.0 10.312161 4 >>END_MODULE