FastQCFastQC Report
Thu 26 May 2016
SRR1513174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781295
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19290.10829200104418414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG4950.011.5415224
TTAGGAC4150.011.4719943
TGGCGAG4600.011.35048218
GCCCTAG1354.4128683E-911.3089451
GCGGCAA600.005915364711.07345212
TAGGACT2600.010.9866414
GGACGTG4700.010.6999096
AGGACGT5250.010.5044725
GCCTTAT1303.6158963E-710.2759171
GTCCTAC5400.010.2487311
TAATACC1502.3590474E-810.1565394
GGCGAGG2900.010.1478319
GACGTGA3100.010.1013347
GGCGAGA2350.010.09907519
TTTAGGA5450.09.964712
GTATCAA17850.09.8893871
GTAGGAC5300.09.8810663
TGCGTTG2800.09.83051412
TCACGTC1451.644039E-79.8163438
TAGACTC1951.6007107E-109.7521625
Produced by FastQC (version 0.11.5)