##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513174_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1781295 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.040341998377585 31.0 30.0 34.0 23.0 34.0 2 28.91501239266938 31.0 30.0 34.0 16.0 34.0 3 29.08399282544441 31.0 30.0 34.0 22.0 34.0 4 32.18234149874108 35.0 33.0 37.0 19.0 37.0 5 32.30352973538914 35.0 33.0 37.0 22.0 37.0 6 32.70253776044956 36.0 33.0 37.0 25.0 37.0 7 32.66878085886953 36.0 33.0 37.0 25.0 37.0 8 32.56554697565535 37.0 33.0 37.0 25.0 37.0 9 33.740086846928776 38.0 34.0 39.0 22.0 39.0 10 33.31105459791893 38.0 33.0 39.0 17.0 39.0 11 33.75872104283681 38.0 34.0 39.0 23.0 39.0 12 33.738258401893006 38.0 34.0 39.0 23.0 39.0 13 33.78188396644015 38.0 34.0 39.0 23.0 39.0 14 33.97588776704588 39.0 33.0 41.0 13.0 41.0 15 34.36508607501845 39.0 33.0 41.0 17.0 41.0 16 34.19413011320416 39.0 33.0 41.0 17.0 41.0 17 33.80619942233038 39.0 33.0 41.0 11.0 41.0 18 33.51809385868147 38.0 32.0 41.0 10.0 41.0 19 34.15918362764169 39.0 33.0 41.0 18.0 41.0 20 34.36058092567486 39.0 34.0 41.0 18.0 41.0 21 34.40968228171078 39.0 34.0 41.0 18.0 41.0 22 34.09628556752251 39.0 34.0 41.0 10.0 41.0 23 33.60719195865929 39.0 33.0 41.0 8.0 41.0 24 33.8361658231792 39.0 34.0 41.0 2.0 41.0 25 33.451922337400596 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9533425580941639 1101 2 1.2698258863982268 1101 3 1.0585555658743075 1101 4 1.434362955189414 1101 5 1.162863206802328 1101 6 1.0095441928978488 1101 7 1.0013181965128979 1101 8 1.111986125764858 1101 9 1.4684958471137861 1101 10 1.7957647649053428 1101 11 1.3409324157157627 1101 12 1.2661400418989217 1101 13 1.131401074102321 1101 14 2.21170506425522 1101 15 1.844488152410669 1101 16 1.8875587100896425 1101 17 2.2928739814743864 1101 18 2.3181858723048663 1101 19 1.8628331272084182 1101 20 1.6049567094969817 1101 21 1.5106346177214647 1101 22 1.9900001659875954 1101 23 2.0072090440924057 1101 24 1.7912917792826093 1101 25 2.100134834037192 1102 1 1.1997417729144324 1102 2 1.294760600806626 1102 3 1.3035526740293086 1102 4 1.5991420357579642 1102 5 1.520200606244991 1102 6 1.256935398183792 1102 7 1.2829751360645716 1102 8 1.3688029931080408 1102 9 1.7671200299484937 1102 10 2.0336637080674436 1102 11 1.592724483297303 1102 12 1.4633989233683309 1102 13 1.417329181083062 1102 14 2.4054902264614384 1102 15 1.9989950490228239 1102 16 2.141150732102723 1102 17 2.45890619487502 1102 18 2.8364658379532557 1102 19 2.311724586626198 1102 20 2.00459391439734 1102 21 1.8888493854099124 1102 22 2.053876640390385 1102 23 2.3379151800616995 1102 24 2.0193286546879037 1102 25 2.37141443163258 1103 1 0.7974192841054588 1103 2 1.108352244127996 1103 3 0.8080550379911315 1103 4 1.4100550574817987 1103 5 1.161462777236892 1103 6 0.8773572456326661 1103 7 0.8599570779113819 1103 8 0.9864399945288227 1103 9 1.34255114862286 1103 10 1.4568803015543068 1103 11 1.14736718658866 1103 12 1.0319671946393 1103 13 1.0023135417430993 1103 14 1.7923563917059795 1103 15 1.4633531404079463 1103 16 1.4733436913227038 1103 17 1.8037642100033082 1103 18 2.1051440796044716 1103 19 1.475968694113547 1103 20 1.2227912028649044 1103 21 1.2715789519232672 1103 22 1.4644351347326605 1103 23 1.9503843763146804 1103 24 1.5673875838279159 1103 25 1.8629729340018955 1104 1 0.9272775197395191 1104 2 1.1987304042688152 1104 3 1.024236558672584 1104 4 1.6386559905003466 1104 5 1.3252098102389667 1104 6 1.0323170385400502 1104 7 1.1820751899869606 1104 8 1.1091276430773576 1104 9 1.50854729980216 1104 10 1.633803162521616 1104 11 1.2162346448785328 1104 12 1.21270629957926 1104 13 1.226032998416322 1104 14 2.1895143241911157 1104 15 1.8256606000538582 1104 16 1.7924941433446122 1104 17 2.2648432358374464 1104 18 2.4629138400357746 1104 19 1.7643900260936647 1104 20 1.52416774158538 1104 21 1.5743814506374108 1104 22 1.756154396475246 1104 23 2.0599210929305585 1104 24 1.9024137590928092 1104 25 2.198682425905652 1105 1 0.8721201603912903 1105 2 1.1163947653291935 1105 3 0.9198879069947381 1105 4 1.3385365580846553 1105 5 1.2053442007977822 1105 6 0.9972973702744312 1105 7 0.8140345216097344 1105 8 0.9339507556299793 1105 9 1.213799681442154 1105 10 1.5069133614046208 1105 11 1.048748401028007 1105 12 0.9896972056841165 1105 13 0.8950938671628492 1105 14 1.7653951647033566 1105 15 1.3754059466591073 1105 16 1.5916673198184341 1105 17 2.0578601949231157 1105 18 1.6065101627021718 1105 19 1.3704592873740253 1105 20 1.1840746816242813 1105 21 1.1167379082656126 1105 22 1.2514199431853328 1105 23 1.6659279404595821 1105 24 1.4662207123020607 1105 25 1.743343960892524 1106 1 0.39870068105555134 1106 2 0.7488319479195802 1106 3 0.6190563238945082 1106 4 1.0817564323608835 1106 5 0.9150624158844209 1106 6 0.6899281825395605 1106 7 0.6612498981269965 1106 8 0.6216377480231401 1106 9 0.8240947147567539 1106 10 0.6064079652449834 1106 11 0.496843917187185 1106 12 0.6175827357300463 1106 13 0.5401706628915477 1106 14 1.5966619672027633 1106 15 1.156900462595388 1106 16 1.1864148414988236 1106 17 1.4697039098334272 1106 18 1.6423831392077162 1106 19 1.1865731001404214 1106 20 0.8476087188181722 1106 21 0.7772544284777965 1106 22 1.107155475376537 1106 23 1.4813225617761105 1106 24 1.1034368946067588 1106 25 1.3996361143018916 1107 1 0.45721654843974946 1107 2 0.6817893362108123 1107 3 0.7323026244399102 1107 4 0.9241959229057173 1107 5 0.8201569675731974 1107 6 0.5578650341314813 1107 7 0.42986422771900834 1107 8 0.4913483178194298 1107 9 0.8431676886676343 1107 10 1.0711493271415051 1107 11 0.5761391432462588 1107 12 0.6539042062923812 1107 13 0.6647789635508943 1107 14 1.4214461117313988 1107 15 0.8639994790092089 1107 16 1.1547686424715309 1107 17 1.4647207172960464 1107 18 1.644121118893736 1107 19 1.0696674860853435 1107 20 0.8374895386287093 1107 21 0.7042168098026096 1107 22 1.069958152139769 1107 23 1.3702990416874385 1107 24 0.9543202575495471 1107 25 1.3564093404542064 1108 1 0.3302924879311675 1108 2 0.6098140838212736 1108 3 0.3844453879317342 1108 4 0.8531995927281457 1108 5 0.7321103074750823 1108 6 0.3815518101788413 1108 7 0.32790521497523173 1108 8 0.4158901741758072 1108 9 0.66711331246605 1108 10 0.9551900781374982 1108 11 0.545538120576964 1108 12 0.29582144511123687 1108 13 0.34312233794418034 1108 14 1.3696765615215583 1108 15 0.9261810956263474 1108 16 0.9848562534781422 1108 17 1.3818333576080093 1108 18 1.5167828240954861 1108 19 0.8489287704323232 1108 20 0.8695989920237821 1108 21 0.8236566134210221 1108 22 0.9065630406496012 1108 23 1.2915278026063604 1108 24 0.9826895296992646 1108 25 1.4120583615968982 1109 1 0.6603775374378849 1109 2 0.7694486916934196 1109 3 0.7052678774723766 1109 4 0.9921150814307964 1109 5 1.109349254572905 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Count 2 88402.0 3 1.0 4 733.0 5 4217.0 6 5219.0 7 4801.0 8 4526.0 9 4210.0 10 3976.0 11 3787.0 12 3679.0 13 3787.0 14 3730.0 15 3682.0 16 4008.0 17 4174.0 18 4817.0 19 5655.0 20 7399.0 21 9409.0 22 11653.0 23 14026.0 24 16266.0 25 18567.0 26 20919.0 27 23544.0 28 26272.0 29 31207.0 30 37620.0 31 44711.0 32 56640.0 33 72995.0 34 94598.0 35 124863.0 36 173810.0 37 248219.0 38 595173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61083918620074 18.564352677830925 12.300046163197305 25.52476197277102 2 14.871240505246384 20.40197317552731 40.3745792145789 24.35220710464741 3 18.33857069538362 24.998649390875286 29.82814624395602 26.834633669785074 4 11.752790218201206 16.818074729714343 38.85885401812838 32.570281033956064 5 13.11239525530666 37.6389866627951 36.03808314749257 13.210534934405665 6 31.793588119559065 37.095663872642824 18.283337376133108 12.827410631665003 7 27.876223711435717 31.618541737438623 22.590520254582515 17.914714296543146 8 25.5322697110207 35.39926264403765 21.076184839844622 17.99228280509703 9 26.778933159492652 14.846926428219689 19.944905530278657 38.429234882009006 10 14.15761743875452 28.43158008812364 35.3421599218825 22.06864255123934 11 34.59485046308098 22.449404738508324 24.18122767278081 18.774517125629878 12 22.963695898892777 24.755455947815626 31.444891880615216 20.835956272676377 13 28.653102527328006 21.612006913847573 25.812539940497786 23.92235061832664 14 21.17225377437212 20.99609956278716 28.563879907413753 29.267766755426976 15 23.669469202990122 29.125934412787203 23.58758347233689 23.617012911885787 16 23.003472953357985 27.545321058675228 26.667059725252052 22.784146262714742 17 22.220830621115255 27.523598403390466 27.60947204838129 22.64609892711299 18 22.063402032908407 27.247655416409277 28.57393159993261 22.115010950749706 19 23.630411221985746 26.87515091616642 27.323772033760296 22.170665828087536 20 23.89528824036803 26.708170104945374 27.27040953852789 22.12613211615871 21 23.919892444132948 26.500626735315524 26.697745100681995 22.881735719869532 22 23.588018595204943 26.910251794621498 26.797865044912577 22.703864565260982 23 23.10542480074172 26.620838993468404 27.179486603520242 23.09424960226963 24 23.167037654257538 26.680495151885857 27.208808441779098 22.943658752077503 25 23.31313013317039 26.484182646514387 27.178845497187424 23.0238417231278 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 161.0 1 161.0 2 294.0 3 427.0 4 427.0 5 427.0 6 832.0 7 1237.0 8 1237.0 9 1237.0 10 1959.5 11 2682.0 12 2682.0 13 2682.0 14 4912.0 15 7142.0 16 7142.0 17 7142.0 18 13193.0 19 19244.0 20 19244.0 21 19244.0 22 30420.0 23 41596.0 24 41596.0 25 41596.0 26 58746.5 27 75897.0 28 75897.0 29 75897.0 30 96610.5 31 117324.0 32 117324.0 33 117324.0 34 137727.0 35 158130.0 36 158130.0 37 158130.0 38 176298.5 39 194467.0 40 194467.0 41 194467.0 42 207733.0 43 220999.0 44 220999.0 45 220999.0 46 228651.0 47 236303.0 48 236303.0 49 236303.0 50 230549.0 51 224795.0 52 224795.0 53 224795.0 54 204082.5 55 183370.0 56 183370.0 57 183370.0 58 158065.5 59 132761.0 60 132761.0 61 132761.0 62 108336.5 63 83912.0 64 83912.0 65 83912.0 66 64665.5 67 45419.0 68 45419.0 69 45419.0 70 33392.5 71 21366.0 72 21366.0 73 21366.0 74 15127.5 75 8889.0 76 8889.0 77 8889.0 78 6197.0 79 3505.0 80 3505.0 81 3505.0 82 2342.5 83 1180.0 84 1180.0 85 1180.0 86 776.0 87 372.0 88 372.0 89 372.0 90 230.0 91 88.0 92 88.0 93 88.0 94 55.0 95 22.0 96 22.0 97 22.0 98 14.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15842406788319732 2 0.23920799193844927 3 0.24246405003101676 4 0.39639700330377614 5 0.23780451862268742 6 0.019704765353296336 7 0.019087237094361123 8 0.018918820296469706 9 0.01987318215118776 10 0.02178190586062387 11 0.022287156254298137 12 0.02986591215941211 13 0.0292483839004769 14 0.026329159403692258 15 0.04294628346231253 16 0.02189418372588482 17 0.04333925599072585 18 0.03340266491513197 19 0.028350160978389317 20 0.03138166334043491 21 0.03587277795087282 22 0.04693214767907618 23 0.031774635868848225 24 0.051198706558992196 25 0.04036389256131073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1781295.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.06502081753726 #Duplication Level Percentage of deduplicated Percentage of total 1 84.20640439396037 64.05161903196556 2 10.871207085745251 16.538371865779418 3 2.684540948235042 6.12598989339089 4 0.9603240734714328 2.9218828256074683 5 0.43632479567259247 1.6594527333021718 6 0.2268158162061787 1.035164986888181 7 0.15245655975019548 0.8117627973818111 8 0.09398756434297276 0.5719332830670275 9 0.07210273253801909 0.4936046266355169 >10 0.27644449205843963 3.543691993056777 >50 0.012162439657145535 0.6309196490248068 >100 0.006111602448260369 0.9160936418893808 >500 8.194970036676285E-4 0.40959799038365347 >1k 2.9799891042459075E-4 0.2899146816273884 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1929 0.10829200104418414 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.61389326304739E-5 0.0 5 5.61389326304739E-5 0.0 0.0 5.61389326304739E-5 0.0 6 5.61389326304739E-5 0.0 0.0 5.61389326304739E-5 0.0 7 5.61389326304739E-5 0.0 0.0 5.61389326304739E-5 0.0 8 5.61389326304739E-5 0.0 0.0 5.61389326304739E-5 0.0 9 5.61389326304739E-5 0.0 0.0 5.61389326304739E-5 0.0 10 5.61389326304739E-5 0.0 0.0 5.61389326304739E-5 0.0 11 5.61389326304739E-5 0.0 0.0 5.61389326304739E-5 0.0 12 5.61389326304739E-5 0.0 0.0 5.61389326304739E-5 0.0 13 5.61389326304739E-5 0.0 0.0 5.61389326304739E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACG 495 0.0 11.541522 4 TTAGGAC 415 0.0 11.471994 3 TGGCGAG 460 0.0 11.350482 18 GCCCTAG 135 4.4128683E-9 11.308945 1 GCGGCAA 60 0.0059153647 11.073452 12 TAGGACT 260 0.0 10.986641 4 GGACGTG 470 0.0 10.699909 6 AGGACGT 525 0.0 10.504472 5 GCCTTAT 130 3.6158963E-7 10.275917 1 GTCCTAC 540 0.0 10.248731 1 TAATACC 150 2.3590474E-8 10.156539 4 GGCGAGG 290 0.0 10.14783 19 GACGTGA 310 0.0 10.101334 7 GGCGAGA 235 0.0 10.099075 19 TTTAGGA 545 0.0 9.96471 2 GTATCAA 1785 0.0 9.889387 1 GTAGGAC 530 0.0 9.881066 3 TGCGTTG 280 0.0 9.830514 12 TCACGTC 145 1.644039E-7 9.816343 8 TAGACTC 195 1.6007107E-10 9.752162 5 >>END_MODULE