FastQCFastQC Report
Thu 26 May 2016
SRR1513174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781295
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26690.14983481119073483No Hit
TATCAACGCAGAGTACTTTTTTTTT18560.10419385896215955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC701.0991278E-412.20466417
TTGCGCG701.0994159E-412.20432218
AATCCCG550.003094710212.07731519
GAAGCGT657.9023663E-411.7136896
CGATACT600.00579320611.1045394
GTATCAA18800.010.739311
TAGGACC2700.010.575754
GTAGGAC5500.010.3837573
GGTATCA8600.010.298711
GTTAACC951.612257E-410.0194153
ATTTGCG951.6577232E-49.99184216
GTCTTAA2201.8189894E-129.9564281
TAGGACG4900.09.9066734
TAATACC1451.5724072E-79.8463894
TTAGACA2350.09.7206914
GTATAAA2450.09.7178971
TAATACT2450.09.7124244
TAGGACT2450.09.7124244
TGTAGGA6900.09.6569372
TCTATAC1304.134643E-69.5184443
Produced by FastQC (version 0.11.5)