FastQCFastQC Report
Thu 26 May 2016
SRR1513173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217730
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC30330.13676146329805702No Hit
CCCATGTACTCTGCGTTGATACCAC26930.1214304716985386No Hit
GTATCAACGCAGAGTACTTTTTTTT23610.10646020931312648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4800.011.1191794
AACACCG700.001477633410.8695985
GTCCTAC8450.010.8519041
TGGCGAG7450.010.06217618
TTAGGAC6550.010.0402283
TAGGACT3500.09.8030324
TAGGGCT2650.09.7105494
GTCCTAA5550.09.6379891
AACTATC1106.9407135E-59.4870877
GTATAAG2950.09.3900391
TATGGCG8350.09.31834116
GTCTTAC1951.5243131E-99.3070141
GTAGGAC9950.09.291473
ATGGCGA8500.09.26553317
CTAGGAC3100.09.2234923
TAGGACG8550.09.1405794
TAAGACA3550.09.1280194
ATAGACC2106.2755134E-109.0770863
GGCGAGG3450.09.076447519
AATCCCG1054.5456388E-49.03715619
Produced by FastQC (version 0.11.5)