##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513173_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2217730
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.52774097838781	31.0	30.0	34.0	25.0	34.0
2	29.389131679690493	31.0	30.0	34.0	23.0	34.0
3	29.572395647802033	31.0	30.0	34.0	24.0	34.0
4	32.65157525938685	37.0	33.0	37.0	23.0	37.0
5	32.8061833496413	37.0	33.0	37.0	25.0	37.0
6	33.192130241282754	37.0	35.0	37.0	27.0	37.0
7	33.17021774517187	37.0	35.0	37.0	27.0	37.0
8	33.05068380731649	37.0	35.0	37.0	26.0	37.0
9	34.312718410266356	39.0	35.0	39.0	25.0	39.0
10	33.88800124451579	39.0	34.0	39.0	22.0	39.0
11	34.35072934937977	39.0	35.0	39.0	27.0	39.0
12	34.3460376150388	39.0	35.0	39.0	27.0	39.0
13	34.38224400625865	39.0	35.0	39.0	27.0	39.0
14	34.61006163960446	40.0	34.0	41.0	17.0	41.0
15	35.04450361405581	40.0	34.0	41.0	24.0	41.0
16	34.89123743647784	40.0	34.0	41.0	21.0	41.0
17	34.50124135940804	39.0	34.0	41.0	17.0	41.0
18	34.228134624142704	39.0	33.0	41.0	17.0	41.0
19	34.86818864334251	40.0	34.0	41.0	22.0	41.0
20	35.08270348509512	40.0	35.0	41.0	24.0	41.0
21	35.13594756800873	40.0	36.0	41.0	25.0	41.0
22	34.82085059948686	40.0	35.0	41.0	18.0	41.0
23	34.367096084735294	40.0	34.0	41.0	16.0	41.0
24	34.61249295450754	40.0	34.0	41.0	18.0	41.0
25	34.2581639784825	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9942240367058197
1101	2	1.2911766291106588
1101	3	1.1175029728590644
1101	4	1.587586839573916
1101	5	1.4854324183774992
1101	6	1.1951693091589988
1101	7	1.1615526590471745
1101	8	1.1742445014178742
1101	9	1.587579131981954
1101	10	1.8937069189901194
1101	11	1.5272199519182266
1101	12	1.3987205102753393
1101	13	1.1543225884573474
1101	14	2.240027734718609
1101	15	1.9005518491914657
1101	16	2.0047580223802015
1101	17	2.3025495402712792
1101	18	2.5022260674642993
1101	19	1.9272065611129534
1101	20	1.5622514151430948
1101	21	1.5303989393801558
1101	22	1.8177847139493792
1101	23	2.109319467672016
1101	24	1.816263268758135
1101	25	2.0648694399848395
1102	1	1.0610789910081806
1102	2	1.2408468046574122
1102	3	1.1258745802138392
1102	4	1.5379551949212313
1102	5	1.467834973746399
1102	6	1.146945968684605
1102	7	1.0769322654155644
1102	8	1.157220781799488
1102	9	1.5002511724239795
1102	10	1.752833346559747
1102	11	1.423086742911245
1102	12	1.214934310500361
1102	13	1.169962278360714
1102	14	2.238713060612916
1102	15	1.8198307820398227
1102	16	1.8786437089236756
1102	17	2.223838629863316
1102	18	2.3544816660032097
1102	19	1.8717640723254476
1102	20	1.6327001459354449
1102	21	1.6080225079574433
1102	22	1.7556259050163732
1102	23	1.989533468257683
1102	24	1.851691269929475
1102	25	2.1073000458446174
1103	1	0.7081078717577114
1103	2	1.0353728331400802
1103	3	0.7776230817023517
1103	4	1.2177703832660782
1103	5	1.0737917830930712
1103	6	0.8256759623420038
1103	7	0.7800495692948388
1103	8	0.7823559668682734
1103	9	1.1667920345990197
1103	10	1.4547830780942945
1103	11	1.0356092896284252
1103	12	1.0385003202409209
1103	13	0.9243844824249337
1103	14	1.7362428737061038
1103	15	1.4256291215348185
1103	16	1.5008680580449436
1103	17	1.8426153073676446
1103	18	2.0734670970621565
1103	19	1.4877261957452745
1103	20	1.1070795517115997
1103	21	1.1770508896793146
1103	22	1.462878281587976
1103	23	1.7998518174284754
1103	24	1.4457563644685507
1103	25	1.6701912444823819
1104	1	0.819026670507526
1104	2	1.0392874319772076
1104	3	0.8984800810078575
1104	4	1.3936326458002881
1104	5	1.1855659666895164
1104	6	0.9098903123761701
1104	7	0.8021495627534918
1104	8	0.8995188244790313
1104	9	1.2253914173004645
1104	10	1.3830900628093588
1104	11	0.9796423099515792
1104	12	0.9672618586346573
1104	13	0.9746596595166892
1104	14	1.909838120516504
1104	15	1.547576715643153
1104	16	1.5705019887744527
1104	17	1.928902010211175
1104	18	2.04774004545839
1104	19	1.37943877938514
1104	20	1.176893021401753
1104	21	1.097510954786543
1104	22	1.4263520774823348
1104	23	1.7040686633789477
1104	24	1.406555911306647
1104	25	1.7459775625001654
1105	1	0.6167494816220405
1105	2	0.8902198747951893
1105	3	0.74871824332903
1105	4	1.1467806763205033
1105	5	0.949443562535528
1105	6	0.6828575760735305
1105	7	0.5633420045759934
1105	8	0.7105150695010494
1105	9	0.917600762688572
1105	10	1.2449405733137553
1105	11	0.8586035194718633
1105	12	0.8344622195655305
1105	13	0.8375385307674748
1105	14	1.5096292518640197
1105	15	1.2021699765924367
1105	16	1.3461283778263393
1105	17	1.691318986748385
1105	18	1.3586696662206847
1105	19	1.178844686431745
1105	20	0.9485178450803531
1105	21	0.9802659569715857
1105	22	1.035900580537053
1105	23	1.2913502337570435
1105	24	1.1575735181029572
1105	25	1.4327563229236162
1106	1	0.6756121312285899
1106	2	0.9417376308650631
1106	3	0.8333665261050847
1106	4	1.2560832065080092
1106	5	1.1436025512004662
1106	6	0.8244501698191442
1106	7	0.7032415803288927
1106	8	0.7850652516209493
1106	9	1.0180514490953811
1106	10	0.7907294369514517
1106	11	0.8110301831065811
1106	12	0.7996008657557212
1106	13	0.7371674329903186
1106	14	1.7754704346485113
1106	15	1.3641200243501217
1106	16	1.5535367076517232
1106	17	1.719466200447016
1106	18	1.803061171894079
1106	19	1.436586417719056
1106	20	1.245343007720443
1106	21	1.177880644232033
1106	22	1.501141600083642
1106	23	1.8658980246085903
1106	24	1.3466475874145516
1106	25	1.7954514538049509
1107	1	0.6633851671774771
1107	2	0.8304699844185599
1107	3	0.7666680292990193
1107	4	1.08229251775996
1107	5	0.8933974162054525
1107	6	0.7073979973675506
1107	7	0.7368299559976492
1107	8	0.8106382275651214
1107	9	1.0174395436424888
1107	10	1.2682270886985165
1107	11	0.7732572586077637
1107	12	0.8015024713945635
1107	13	0.780298252634239
1107	14	1.7137892415799882
1107	15	1.0477272796603856
1107	16	1.1627779689633542
1107	17	1.6295654935415271
1107	18	1.775344877403775
1107	19	1.2471961967046497
1107	20	1.000143318175752
1107	21	0.8083382537529005
1107	22	1.0671528957709242
1107	23	1.4508575258829097
1107	24	1.34503726767322
1107	25	1.5326725221474717
1108	1	0.4623068543549387
1108	2	0.6389934569150135
1108	3	0.46652542601865576
1108	4	0.9266788283342535
1108	5	0.9003501560247003
1108	6	0.4796625503887384
1108	7	0.4745840609087253
1108	8	0.5283095030677742
1108	9	0.6787658380136108
1108	10	0.9833709211066477
1108	11	0.610018521812151
1108	12	0.4475193549101135
1108	13	0.39155440722723966
1108	14	1.3000978590497496
1108	15	0.8599212753319705
1108	16	0.8565987497311625
1108	17	1.3137779371366136
1108	18	1.5133585230805693
1108	19	0.8627451258609398
1108	20	0.713940526247427
1108	21	0.6786049107053529
1108	22	0.9571197538740179
1108	23	1.2400366939232796
1108	24	0.9346836615067247
1108	25	1.302930023976316
1109	1	0.4434124861003923
1109	2	0.654274625621003
1109	3	0.7001714251941138
1109	4	0.8559751038912751
1109	5	0.9100160667192654
1109	6	0.578493099148254
1109	7	0.5378675865189635
1109	8	0.47404021739930613
1109	9	0.7245912232607665
1109	10	0.9432440818169923
1109	11	0.573375653889137
1109	12	0.4481942510043595
1109	13	0.5321328900798719
1109	14	1.4557612437119403
1109	15	0.8940809033589829
1109	16	1.0276516880261113
1109	17	1.3738797611171165
1109	18	1.5151070595012257
1109	19	0.9715671654678886
1109	20	0.776088477287388
1109	21	0.694965589878997
1109	22	0.9040575053649107
1109	23	1.2076850693999788
1109	24	0.9293101852550194
1109	25	1.1888736049428275
1110	1	0.46548625897288787
1110	2	0.7048119477238188
1110	3	0.6002585585080844
1110	4	0.980285298489008
1110	5	0.7706027643666644
1110	6	0.5288271101851407
1110	7	0.5050473715901234
1110	8	0.6227477399087462
1110	9	0.7893603199787478
1110	10	1.068716053934331
1110	11	0.6332071961487955
1110	12	0.5591440041266367
1110	13	0.4174073600188777
1110	14	1.1295922050828366
1110	15	0.9025037903761159
1110	16	1.1571898814620667
1110	17	1.4781492935016658
1110	18	0.9632627376888507
1110	19	0.8118227565222043
1110	20	0.5809098539937452
1110	21	0.5137282968206165
1110	22	0.8831455100120209
1110	23	-0.16669773629273266
1110	24	0.5386624942878342
1110	25	0.8966865587621768
1111	1	0.2223410445964511
1111	2	0.6431764912955593
1111	3	0.5785326131544757
1111	4	0.8583156302248796
1111	5	0.6821824045601019
1111	6	0.40424100602894697
1111	7	0.3030985460919098
1111	8	0.4374941312192462
1111	9	0.6467109614558666
1111	10	0.8210142990604794
1111	11	0.4337849931084321
1111	12	0.37329631611683567
1111	13	0.2811786958555089
1111	14	1.1895755648126922
1111	15	0.7869987570010579
1111	16	0.8511945868794655
1111	17	1.2782978511390795
1111	18	1.479931783417328
1111	19	0.8990232835397052
1111	20	0.5360101102350896
1111	21	0.4147742242518646
1111	22	0.8308211610186902
1111	23	1.1667857621900595
1111	24	0.7774859937502612
1111	25	1.0345695880642936
1112	1	0.20193738039974107
1112	2	0.3508029610330041
1112	3	0.2973325240051672
1112	4	0.8080734724112588
1112	5	0.4893222899004783
1112	6	0.23871848331705792
1112	7	0.15582535717911838
1112	8	0.29010350010420183
1112	9	0.5004850767273936
1112	10	0.6830342248650325
1112	11	0.23997800069312802
1112	12	0.20272436258257187
1112	13	0.1975902093284958
1112	14	0.7349032502437538
1112	15	0.561518403379921
1112	16	0.43220134681259026
1112	17	0.9009915882333601
1112	18	0.9869306046579567
1112	19	0.6140617074920414
1112	20	0.3671707628267029
1112	21	0.21412981021338595
1112	22	0.40406881159076846
1112	23	0.744949803589769
1112	24	0.39591494405385674
1112	25	0.7868361057928936
1113	1	0.020159460456589784
1113	2	0.1381678637290129
1113	3	0.12713320859465327
1113	4	0.4205675631777481
1113	5	0.4340404747702493
1113	6	0.16242017356577065
1113	7	0.19054553480377479
1113	8	0.13071837310849332
1113	9	0.308637340500276
1113	10	0.5257328408152588
1113	11	0.17843647337791424
1113	12	0.14492399137791523
1113	13	-0.019994671550335852
1113	14	0.585332025225135
1113	15	0.32866017075722453
1113	16	0.43693322480275754
1113	17	0.8024874518530751
1113	18	0.7187738297404493
1113	19	0.40568433560638795
1113	20	0.27646298885368026
1113	21	0.2018485017552223
1113	22	0.16735073916693466
1113	23	0.34235617820972664
1113	24	0.26037966647042055
1113	25	0.6619813623209936
1114	1	-0.7676078827909016
1114	2	-0.5115922920949387
1114	3	-0.44574884321272634
1114	4	-0.1351825417938386
1114	5	-3.209681130121151
1114	6	-1.0571826598121277
1114	7	-0.8526354127130205
1114	8	-0.645965785716335
1114	9	-0.3493645337116291
1114	10	-0.12221591181592117
1114	11	-0.5129955190124278
1114	12	-0.5761900195595544
1114	13	-0.5719020180865755
1114	14	-6.149819205586503
1114	15	-2.281113285925393
1114	16	-1.1704660626466108
1114	17	-0.23647412809961565
1114	18	-6.224460792884155
1114	19	-2.3655854692967893
1114	20	-1.304919553226334
1114	21	-1.0422017507236347
1114	22	-0.7828259072904515
1114	23	-0.33819685936941823
1114	24	-0.44814982926965286
1114	25	-0.20549404280126282
1115	1	-0.6891182026326206
1115	2	-0.37009123290503965
1115	3	-0.3095631309302007
1115	4	-3.176380447116088
1115	5	-1.007230435803816
1115	6	-0.7527395284363649
1115	7	-0.6564798055204477
1115	8	-0.4653521001310281
1115	9	-0.18697462352507443
1115	10	-0.6380172880681272
1115	11	-0.614406565091798
1115	12	-0.6246802136923648
1115	13	-0.6752927391685404
1115	14	-5.899173795549849
1115	15	-2.3446037924666427
1115	16	-1.2671942465726573
1115	17	-0.49086874316495965
1115	18	-0.22112329891310623
1115	19	-0.5235948284920298
1115	20	-0.5302251839653209
1115	21	-0.6542905986227865
1115	22	-1.8511425359455274
1115	23	-0.9682198500497776
1115	24	-0.7327291737646107
1115	25	-0.40904739088978914
1116	1	-0.2806506774478308
1116	2	0.018223775512641538
1116	3	-0.032148740597204295
1116	4	0.11358466633075182
1116	5	-0.5074984595154248
1116	6	-0.23755543499838438
1116	7	-0.18534519852430265
1116	8	-0.10657824951872641
1116	9	-0.07884831575479012
1116	10	0.09394127245028017
1116	11	-0.23818128715473819
1116	12	-0.5084665994734436
1116	13	-0.4959596087887945
1116	14	0.3194565285686579
1116	15	-0.045333990669703894
1116	16	-0.12907685979433836
1116	17	0.3919566016897278
1116	18	0.2483093643066141
1116	19	0.10994346894474205
1116	20	-0.3646631472078994
1116	21	-0.34885631938174555
1116	22	-0.3207189919167561
1116	23	-0.908400978653745
1116	24	-0.654509448931087
1116	25	-0.07530271655354426
1201	1	0.932672753397501
1201	2	1.1952871376823886
1201	3	1.1437085963364773
1201	4	1.4785987789043133
1201	5	1.458974130611736
1201	6	1.141858087093489
1201	7	1.0083251555966086
1201	8	1.2154208610911468
1201	9	1.4317233542661896
1201	10	1.860610536991885
1201	11	1.3658513788303637
1201	12	1.3032982592250875
1201	13	1.1923357420847793
1201	14	2.1053312712644257
1201	15	1.7050230643181408
1201	16	1.8890903852815413
1201	17	2.213920564579844
1201	18	1.628718483764544
1201	19	1.5778629225963599
1201	20	1.5333939479706018
1201	21	1.24965723770503
1201	22	1.6022824399707787
1201	23	1.9826417013751652
1201	24	1.6912512012100365
1201	25	1.9937940328936463
1202	1	0.9267300924340311
1202	2	1.069933304447538
1202	3	0.9600798024868098
1202	4	1.40657135360496
1202	5	1.240985349394279
1202	6	0.9072626482642647
1202	7	0.8842740874233712
1202	8	0.9899791633793313
1202	9	1.2230706487913565
1202	10	1.5524403169868393
1202	11	1.2576237890226452
1202	12	1.0756229397322912
1202	13	1.0725591766537974
1202	14	1.986045063815581
1202	15	1.5395402304670611
1202	16	1.6227910723618422
1202	17	2.081399849466159
1202	18	2.1416642421544196
1202	19	1.5722534408338973
1202	20	1.461919227331677
1202	21	1.3572845258236015
1202	22	1.5245005700952703
1202	23	1.7579480733142745
1202	24	1.3250045526833247
1202	25	1.8091695019641705
1203	1	0.5390792614677906
1203	2	0.770171857669375
1203	3	0.7022175342713908
1203	4	1.0648739086603953
1203	5	0.9995761326282704
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2211	13	-0.7581621874208153
2211	14	-0.13084539001105355
2211	15	-0.3050556074980406
2211	16	-6.8685751497007494
2211	17	-2.153974920766352
2211	18	-0.8728770866322719
2211	19	-0.7882069374186997
2211	20	-0.9170387993035973
2211	21	-0.7879957836831082
2211	22	-0.5921951959010556
2211	23	-0.14987760929659544
2211	24	-0.5564886833442131
2211	25	-7.727376075301887
2212	1	-1.7509804688285158
2212	2	-4.0472472702954825
2212	3	-2.0500117342533244
2212	4	-4.04489427119643
2212	5	-1.5713774625715615
2212	6	-1.3709966651349177
2212	7	-1.3959967596385248
2212	8	-1.209301655216354
2212	9	-1.4445931991302103
2212	10	-0.7662695217492796
2212	11	-1.3334681457760382
2212	12	-1.5084448506413821
2212	13	-1.6491109298185052
2212	14	-7.026940509070943
2212	15	-5.718989093736575
2212	16	-3.4381072855630315
2212	17	-7.923088929631415
2212	18	-3.5081936777053535
2212	19	-2.504511313256245
2212	20	-2.477909456967758
2212	21	-2.0591018552221243
2212	22	-1.7443740949836268
2212	23	-1.3710970712077355
2212	24	-1.799064191023838
2212	25	-1.5499143445524837
2213	1	-0.43974729036748883
2213	2	-0.5602833845431157
2213	3	-0.5766107445514059
2213	4	-0.15704465158997039
2213	5	-0.39228198471953135
2213	6	-0.5881442997843607
2213	7	-0.6520343115454281
2213	8	-0.66312194163757
2213	9	-0.5561646594341525
2213	10	-4.259492315744428
2213	11	-2.0284408117392942
2213	12	-1.4670899339083832
2213	13	-1.1956497497038612
2213	14	-0.366404255993551
2213	15	-0.8866991431599658
2213	16	-0.7217094858604369
2213	17	-0.26693584051505326
2213	18	-0.23582619842184016
2213	19	-0.7587234081047285
2213	20	-1.0937874163554255
2213	21	-0.9391382903207557
2213	22	-1.1436876589594647
2213	23	-1.4280800537645462
2213	24	-0.9589064495680333
2213	25	-0.6341900234299942
2214	1	-1.2752072352620125
2214	2	-0.9389762912318922
2214	3	-0.8849272817928693
2214	4	-0.4448394319363942
2214	5	-3.7553491655133833
2214	6	-1.716603431080955
2214	7	-1.4560152831219142
2214	8	-1.1631471059950726
2214	9	-1.1868949856714366
2214	10	-4.5081106585062045
2214	11	-2.2716313961599113
2214	12	-1.620579560237033
2214	13	-1.4499987165059665
2214	14	-0.6796964865502915
2214	15	-0.8852580742137945
2214	16	-0.8742500138771518
2214	17	-0.8047988177593268
2214	18	-0.37101448737656284
2214	19	-1.1164815050717891
2214	20	-1.3325452024160995
2214	21	-1.389057496354006
2214	22	-1.1769120559380752
2214	23	-0.9317687912106649
2214	24	-1.478494593909602
2214	25	-1.0413344376573548
2215	1	-2.194934454917334
2215	2	-4.773705269225392
2215	3	-2.986274441583447
2215	4	-1.8410954482964854
2215	5	-1.8708290409557229
2215	6	-2.0715852151616723
2215	7	-2.0713579153367583
2215	8	-2.3156151918669714
2215	9	-8.298137875427692
2215	10	-3.882173748608558
2215	11	-3.0951187610783357
2215	12	-3.5138755622518687
2215	13	-2.8109621871753774
2215	14	-7.837293639086994
2215	15	-4.733488826213833
2215	16	-9.299329864482242
2215	17	-4.611652332520663
2215	18	-3.1653231587112387
2215	19	-3.461119124920234
2215	20	-3.338650494955985
2215	21	-3.414706456613242
2215	22	-9.40400491360321
2215	23	-10.686867536697303
2215	24	-6.761330108626833
2215	25	-5.038129967880465
2216	1	-1.8004055138883466
2216	2	-1.739209926519397
2216	3	-1.539545757608039
2216	4	-6.979148230378026
2216	5	-3.495061319410045
2216	6	-2.725346318096417
2216	7	-2.2084537865669027
2216	8	-7.672655917437684
2216	9	-3.8736123661812165
2216	10	-2.224031767129407
2216	11	-2.0724543774161006
2216	12	-2.2679064245805307
2216	13	-2.093396607724898
2216	14	-1.4208739138963793
2216	15	-2.06509838610625
2216	16	-1.8057555302810613
2216	17	-1.506346125764054
2216	18	-7.249818844999698
2216	19	-4.5190263789431455
2216	20	-3.991585787329168
2216	21	-3.1358107333526135
2216	22	-2.8155526394308694
2216	23	-2.1252526910244427
2216	24	-2.455732975101661
2216	25	-1.9136645249332744
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95286.0
3	1.0
4	778.0
5	4854.0
6	5796.0
7	5133.0
8	4860.0
9	4664.0
10	4261.0
11	4096.0
12	4133.0
13	3978.0
14	4118.0
15	4112.0
16	4219.0
17	4576.0
18	5146.0
19	6194.0
20	7949.0
21	10173.0
22	12650.0
23	15201.0
24	17511.0
25	20343.0
26	22885.0
27	26133.0
28	29091.0
29	34430.0
30	42546.0
31	50543.0
32	64119.0
33	83357.0
34	110310.0
35	148600.0
36	211748.0
37	308728.0
38	835208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95805801528924	18.29971103461171	12.188486007444991	25.553744942654056
2	14.726642954143934	20.22221900750977	40.78936661525136	24.261771423094935
3	18.486450515062167	24.881123850909102	29.59683828774945	27.035587346279282
4	11.467715974690849	16.806339337770783	39.04765874053138	32.678285947006984
5	12.962839901142681	37.72364162730321	36.15565823432006	13.157860237234056
6	31.886290738662808	36.9449565778982	18.35445207332869	12.814300610110296
7	28.033981731910167	31.40509509500029	22.513055239481734	18.04786793360781
8	25.397279252723543	35.737589138658535	21.007499102525014	17.85763250609291
9	26.677998772380267	14.881664583802184	19.95391690520764	38.486419738609904
10	13.877316678370422	28.54927100711381	35.66344057942385	21.90997173509192
11	34.582101891035336	22.400395805436553	24.209122016406802	18.808380287121302
12	23.062101166753568	24.760497083567124	31.606221122082438	20.57118062759687
13	28.96718669588777	21.48644254097322	25.61044310206173	23.93592766107728
14	20.71903404747225	21.16433163191439	28.738224285113134	29.378410035500224
15	23.771802961584925	29.217660162054433	23.49053990777639	23.51999696858425
16	22.765018193757655	27.544374908670406	27.049075338573473	22.64153155899847
17	22.063887738595632	27.58894924545273	27.836781055436916	22.510381960514717
18	21.949934551726688	27.081061137076922	28.925385359974527	22.043618951221866
19	23.586762135648645	26.890049050008457	27.28614760105993	22.237041213282968
20	23.85360205642355	26.86083733677093	27.263985118984525	22.021575487820993
21	23.658839131062358	26.480134959584294	26.824209728637417	23.036816180715935
22	23.403846431458597	27.014657798244603	26.86425431631828	22.71724145397852
23	22.793742549284662	26.723299996526944	27.430314527874415	23.052642926313975
24	23.173157954572112	26.851609573529334	27.270221400948834	22.70501107094972
25	23.118970742000762	26.545199643826646	27.173645336862588	23.162184277310008
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	249.0
1	249.0
2	375.5
3	502.0
4	502.0
5	502.0
6	987.5
7	1473.0
8	1473.0
9	1473.0
10	2318.0
11	3163.0
12	3163.0
13	3163.0
14	6085.0
15	9007.0
16	9007.0
17	9007.0
18	16392.5
19	23778.0
20	23778.0
21	23778.0
22	38024.0
23	52270.0
24	52270.0
25	52270.0
26	73867.0
27	95464.0
28	95464.0
29	95464.0
30	122106.0
31	148748.0
32	148748.0
33	148748.0
34	173492.0
35	198236.0
36	198236.0
37	198236.0
38	220746.5
39	243257.0
40	243257.0
41	243257.0
42	258860.5
43	274464.0
44	274464.0
45	274464.0
46	286024.5
47	297585.0
48	297585.0
49	297585.0
50	289114.5
51	280644.0
52	280644.0
53	280644.0
54	252892.0
55	225140.0
56	225140.0
57	225140.0
58	193649.5
59	162159.0
60	162159.0
61	162159.0
62	132220.0
63	102281.0
64	102281.0
65	102281.0
66	79010.5
67	55740.0
68	55740.0
69	55740.0
70	40989.5
71	26239.0
72	26239.0
73	26239.0
74	18511.5
75	10784.0
76	10784.0
77	10784.0
78	7536.0
79	4288.0
80	4288.0
81	4288.0
82	2936.0
83	1584.0
84	1584.0
85	1584.0
86	1027.5
87	471.0
88	471.0
89	471.0
90	314.0
91	157.0
92	157.0
93	157.0
94	95.5
95	34.0
96	34.0
97	34.0
98	23.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1634103339901611
2	0.25602755971195773
3	0.2599053987636006
4	0.46475450122422474
5	0.2549002809178755
6	0.019208830651161322
7	0.018983374892344872
8	0.017946278401789216
9	0.01943428640997777
10	0.02222993781930172
11	0.021598661694615665
12	0.028768154824978697
13	0.027550693727369877
14	0.025296136139205404
15	0.04256604726454528
16	0.021914299756958692
17	0.0407624011940137
18	0.03210490005546212
19	0.02728014681679014
20	0.030526709743746983
21	0.03472018685773291
22	0.046263521709135016
23	0.02980525131553435
24	0.05041190767135765
25	0.03751583826705686
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2217730.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.53237215929407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.5195699645452	60.678597290578416
2	11.879592494626795	17.470692328313085
3	3.096990751416115	6.831872295210648
4	1.1132298127623328	3.2743371556344445
5	0.4807349052894168	1.7674788982852192
6	0.24750639079999126	1.0919839224065182
7	0.1668434695448518	0.8587877266443922
8	0.09885208557694562	0.5815062676293081
9	0.07458267996410717	0.49358172417824286
>10	0.29551099627415844	3.646778974670288
>50	0.016613378357164303	0.8306735670167429
>100	0.008302599191579038	1.196693860430081
>500	0.0010517784471848533	0.5323705416213504
>1k	6.18693204226023E-4	0.7446454473811562
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3033	0.13676146329805702	No Hit
CCCATGTACTCTGCGTTGATACCAC	2693	0.1214304716985386	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2361	0.10646020931312648	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.509115176328949E-5	0.0
4	0.0	0.0	0.0	4.509115176328949E-5	0.0
5	0.0	0.0	0.0	4.509115176328949E-5	0.0
6	0.0	0.0	0.0	9.018230352657898E-5	0.0
7	0.0	0.0	0.0	9.018230352657898E-5	0.0
8	1.8036460705315795E-4	0.0	0.0	9.018230352657898E-5	0.0
9	1.8036460705315795E-4	0.0	0.0	1.3527345528986846E-4	0.0
10	1.8036460705315795E-4	0.0	0.0	1.3527345528986846E-4	0.0
11	2.2545575881644745E-4	0.0	0.0	1.3527345528986846E-4	0.0
12	2.2545575881644745E-4	0.0	0.0	1.3527345528986846E-4	9.018230352657898E-5
13	2.705469105797369E-4	0.0	0.0	1.3527345528986846E-4	9.018230352657898E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	480	0.0	11.119179	4
AACACCG	70	0.0014776334	10.869598	5
GTCCTAC	845	0.0	10.851904	1
TGGCGAG	745	0.0	10.062176	18
TTAGGAC	655	0.0	10.040228	3
TAGGACT	350	0.0	9.803032	4
TAGGGCT	265	0.0	9.710549	4
GTCCTAA	555	0.0	9.637989	1
AACTATC	110	6.9407135E-5	9.487087	7
GTATAAG	295	0.0	9.390039	1
TATGGCG	835	0.0	9.318341	16
GTCTTAC	195	1.5243131E-9	9.307014	1
GTAGGAC	995	0.0	9.29147	3
ATGGCGA	850	0.0	9.265533	17
CTAGGAC	310	0.0	9.223492	3
TAGGACG	855	0.0	9.140579	4
TAAGACA	355	0.0	9.128019	4
ATAGACC	210	6.2755134E-10	9.077086	3
GGCGAGG	345	0.0	9.0764475	19
AATCCCG	105	4.5456388E-4	9.037156	19
>>END_MODULE