FastQCFastQC Report
Thu 26 May 2016
SRR1513173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217730
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC34010.15335500714694755No Hit
CCCATGTACTCTGCGTTGATACCAC33120.1493418946400148No Hit
GTATCAACGCAGAGTACTTTTTTTT31300.1411353050190961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC1001.464814E-712.3361068
TGCACCG951.0170916E-612.0225545
TAGGACC4250.011.1974784
GTCCTAC10050.011.1807571
CCGACCT600.00579541811.1041654
GAACCGT951.33444755E-511.0194296
GTGCGCC1408.483767E-910.8466411
CTAGTAG1252.1806045E-710.6612043
GCGCCAC1252.2649692E-710.631865513
CTAGCAC2300.010.3466653
GTCCTAT2950.010.3295941
ACCGCAT750.002673176810.1219338
TGGCGAG7600.09.9925818
TGTACCG1252.5098689E-69.898575
TCCTACA12050.09.7969672
TATGGCG8000.09.72983616
GTATTAA2500.09.5225941
CTACACG1106.6991524E-59.5178574
TGCGCCA1401.0699696E-69.49273712
ACCGCTC800.0045588669.4893128
Produced by FastQC (version 0.11.5)