##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513173_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2217730 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.82794073219012 31.0 31.0 34.0 27.0 34.0 2 30.93341569983722 33.0 31.0 34.0 27.0 34.0 3 31.044968052918975 33.0 31.0 34.0 27.0 34.0 4 34.44372353713031 37.0 35.0 37.0 31.0 37.0 5 34.19735630577212 37.0 35.0 37.0 30.0 37.0 6 34.11336862467478 37.0 35.0 37.0 30.0 37.0 7 33.98414054010182 37.0 35.0 37.0 30.0 37.0 8 33.95317419162838 37.0 35.0 37.0 30.0 37.0 9 35.515679095291134 39.0 37.0 39.0 30.0 39.0 10 35.366908956455475 39.0 35.0 39.0 29.0 39.0 11 35.379845607896364 39.0 35.0 39.0 30.0 39.0 12 35.31445712507835 39.0 35.0 39.0 29.0 39.0 13 35.284605880788014 39.0 35.0 39.0 29.0 39.0 14 36.4607824216654 40.0 37.0 41.0 29.0 41.0 15 36.43480676186912 40.0 37.0 41.0 29.0 41.0 16 36.398370405775275 40.0 37.0 41.0 29.0 41.0 17 36.333509940344406 40.0 37.0 41.0 28.0 41.0 18 36.28157169718586 40.0 36.0 41.0 28.0 41.0 19 36.359688961235136 40.0 37.0 41.0 28.0 41.0 20 36.32524202675709 40.0 37.0 41.0 28.0 41.0 21 36.274650205390195 40.0 37.0 41.0 28.0 41.0 22 36.22128933639352 40.0 37.0 41.0 28.0 41.0 23 36.14407524811406 40.0 37.0 41.0 28.0 41.0 24 36.07925175742764 40.0 37.0 41.0 27.0 41.0 25 36.02332655463019 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5316942682765635 1101 2 0.5761975317985453 1101 3 0.5229007041939155 1101 4 0.701992945963319 1101 5 0.5836685206063663 1101 6 0.5771796028729241 1101 7 0.6857395488821894 1101 8 0.7295787428629694 1101 9 0.7432668771378985 1101 10 0.7324867811368776 1101 11 0.7359581378613882 1101 12 0.7312394116572847 1101 13 0.6864173160940794 1101 14 0.7214935734965877 1101 15 0.880631478147933 1101 16 0.76372886921353 1101 17 0.8235292666903433 1101 18 0.8154654564709318 1101 19 0.9045050011647291 1101 20 0.8680071653210817 1101 21 0.9547295653451471 1101 22 0.9553217017074331 1101 23 0.9068921077694654 1101 24 1.010830880197794 1101 25 0.8725538216516711 1102 1 0.545516841215548 1102 2 0.5544899966969012 1102 3 0.4900839417137597 1102 4 0.5518808210615731 1102 5 0.63123452402791 1102 6 0.5618932412681872 1102 7 0.5160895635239271 1102 8 0.5310733562439793 1102 9 0.5001442564206044 1102 10 0.6304592854908719 1102 11 0.6327095981822737 1102 12 0.6760377409577174 1102 13 0.7109341386824326 1102 14 0.7456435819733827 1102 15 0.6782599016178779 1102 16 0.73104927671627 1102 17 0.9259447684396491 1102 18 0.7830174346470358 1102 19 0.8741099053769261 1102 20 0.9247916851490814 1102 21 0.8615626340996769 1102 22 0.8406687880012242 1102 23 0.8302765073810718 1102 24 0.8274280628188251 1102 25 0.8886061774305389 1103 1 0.4590308720828844 1103 2 0.37827018834637727 1103 3 0.42398849029372343 1103 4 0.476629450164026 1103 5 0.5127536442154792 1103 6 0.4021732491154921 1103 7 0.4777588110397417 1103 8 0.41157289816706566 1103 9 0.5026680602303557 1103 10 0.5719958145058968 1103 11 0.5400083538510998 1103 12 0.5508290350202429 1103 13 0.6982285516678175 1103 14 0.7177959339745428 1103 15 0.6108944057615204 1103 16 0.6109725559755717 1103 17 0.6692220849660302 1103 18 0.6241788358100351 1103 19 0.4922289901373489 1103 20 0.5978977132889085 1103 21 0.5231831216611269 1103 22 0.5374857724647057 1103 23 0.44238602300044505 1103 24 0.6510954970738325 1103 25 0.5710759262596312 1104 1 0.3902265319720719 1104 2 0.47133817515791776 1104 3 0.45506923596959226 1104 4 0.6030704358932297 1104 5 0.513156795008264 1104 6 0.5196144617040019 1104 7 0.4793181556251085 1104 8 0.6578824446757778 1104 9 0.5692307149203373 1104 10 0.6941971755689593 1104 11 0.6981581468709734 1104 12 0.681292342962962 1104 13 0.6586202156785106 1104 14 0.7304341778144305 1104 15 0.7234117043635706 1104 16 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-0.878309252343648 2215 21 -1.1008667050028436 2215 22 -1.0658296882822995 2215 23 -1.0501070969644246 2215 24 -1.1106020393050784 2215 25 -1.1170083106705206 2216 1 -0.4778113721825221 2216 2 -0.40806320344512415 2216 3 -0.5546948974165176 2216 4 -0.2593078276978318 2216 5 -0.485987149878099 2216 6 -0.5700819797049874 2216 7 -0.7258132223569405 2216 8 -0.6678685846285859 2216 9 -0.898601385508222 2216 10 -0.9215162170266069 2216 11 -0.8593048669382952 2216 12 -0.8670490169188767 2216 13 -0.9684740239125347 2216 14 -1.0907691682295635 2216 15 -1.0369303519440507 2216 16 -1.0592671634375819 2216 17 -1.0518864332383586 2216 18 -1.0864783351498275 2216 19 -1.0855768609459915 2216 20 -1.0048256805034512 2216 21 -1.280277586380791 2216 22 -1.0952172397371456 2216 23 -1.081425177661572 2216 24 -1.0754740332785033 2216 25 -1.1802901376237926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49481.0 3 1.0 4 787.0 5 6800.0 6 7814.0 7 6034.0 8 4782.0 9 4397.0 10 3914.0 11 3577.0 12 3255.0 13 3233.0 14 3098.0 15 3010.0 16 3094.0 17 3386.0 18 3682.0 19 4338.0 20 5649.0 21 7361.0 22 9409.0 23 11434.0 24 13273.0 25 14666.0 26 16504.0 27 18400.0 28 20702.0 29 24977.0 30 31687.0 31 40078.0 32 51883.0 33 67388.0 34 89116.0 35 121021.0 36 173186.0 37 284139.0 38 1102174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.12015970101455 19.083132969297893 13.191685031801534 27.605022297886027 2 14.05130078331101 20.599411051133927 39.115539501512956 26.2337486640421 3 16.806171334158247 24.815792511571026 29.993263011316696 28.384773142954028 4 11.395983903870539 17.306721090005933 37.4479860899428 33.84930891618072 5 12.885332794233314 37.971849773402 35.89453976216122 13.248277670203457 6 31.968082532011778 37.341139763536454 17.91994579403997 12.770831910411806 7 27.730479564194923 31.496325442980993 22.58429560992595 18.188899382898132 8 25.445624478567723 36.080046900719296 20.540846467789578 17.933482152923403 9 26.330769931449215 14.723718066076067 19.57597899736024 39.36953300511448 10 14.32799854259746 28.906488146880204 34.85640616798729 21.90910714253505 11 34.558940899027384 22.32386268842465 24.12904185811618 18.988154554431784 12 22.777389492859815 24.886032113918287 31.568360440630734 20.768217952591165 13 28.766351178908163 21.428352414405722 25.704526700725516 24.100769705960598 14 21.307254498608007 21.229966731088172 27.648098062575134 29.814680707728687 15 23.70780031834353 29.313261758645098 23.33985651995509 23.639081403056277 16 22.98922772384375 27.643175679636382 26.481762883669337 22.885833712850527 17 22.329264706678238 27.8984454911667 27.103827551806436 22.66846225034862 18 22.354607907388555 27.357379557266707 28.126946525875574 22.161066009469167 19 23.497269730760735 26.96550075978591 27.218011209660332 22.319218299793032 20 23.751358370946868 26.785181243884516 27.310944073444464 22.15251631172415 21 23.55553832057544 26.414791460748738 26.960398825456146 23.06927139321968 22 23.31109887637308 27.118146536389254 26.715617643093452 22.855136944144213 23 22.884111632848438 26.813691072880435 26.906539573798778 23.39565772047235 24 23.034950151731724 26.919552876139115 27.22513561163893 22.82036136049023 25 23.131486937520684 26.60941883713317 26.912341741044653 23.346752484301486 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 188.0 1 188.0 2 295.5 3 403.0 4 403.0 5 403.0 6 949.0 7 1495.0 8 1495.0 9 1495.0 10 2317.0 11 3139.0 12 3139.0 13 3139.0 14 5647.5 15 8156.0 16 8156.0 17 8156.0 18 15563.0 19 22970.0 20 22970.0 21 22970.0 22 36822.0 23 50674.0 24 50674.0 25 50674.0 26 72037.5 27 93401.0 28 93401.0 29 93401.0 30 119527.0 31 145653.0 32 145653.0 33 145653.0 34 170423.5 35 195194.0 36 195194.0 37 195194.0 38 217480.0 39 239766.0 40 239766.0 41 239766.0 42 255373.5 43 270981.0 44 270981.0 45 270981.0 46 285124.0 47 299267.0 48 299267.0 49 299267.0 50 292794.0 51 286321.0 52 286321.0 53 286321.0 54 257109.0 55 227897.0 56 227897.0 57 227897.0 58 196844.0 59 165791.0 60 165791.0 61 165791.0 62 135420.0 63 105049.0 64 105049.0 65 105049.0 66 81252.5 67 57456.0 68 57456.0 69 57456.0 70 42038.5 71 26621.0 72 26621.0 73 26621.0 74 18686.0 75 10751.0 76 10751.0 77 10751.0 78 7538.5 79 4326.0 80 4326.0 81 4326.0 82 2954.5 83 1583.0 84 1583.0 85 1583.0 86 1028.0 87 473.0 88 473.0 89 473.0 90 314.5 91 156.0 92 156.0 93 156.0 94 87.5 95 19.0 96 19.0 97 19.0 98 9.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1732852962263215 2 0.009920053387923688 3 0.005501120515121317 4 0.0024349221952176324 5 0.07624913763172252 6 0.31126422062198733 7 0.026693961843867374 8 0.012625522493721057 9 0.004689479783382107 10 0.0038778390516428962 11 0.0 12 0.0 13 0.0 14 0.0019840106775847375 15 0.0 16 0.0 17 0.019749924472320796 18 0.028813245976741987 19 0.0 20 0.0 21 8.116407317392109E-4 22 4.5091151763289496E-5 23 0.006267670095097239 24 0.0 25 1.8036460705315798E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2217730.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.6422194583032 #Duplication Level Percentage of deduplicated Percentage of total 1 80.51482572528042 56.877459885322956 2 13.047434756085208 18.433994988145276 3 3.5330701588035947 7.487517525593569 4 1.261835428877913 3.565554211482227 5 0.5742261968713326 2.0282306509045744 6 0.2926445207972355 1.2403835076856973 7 0.19079519130029238 0.9434737042796937 8 0.12347250796786578 0.6977897603946452 9 0.08037468070212878 0.5110061249745745 >10 0.34910386528170395 4.169298463453411 >50 0.019430847736509053 0.9411159501643954 >100 0.010679452579678263 1.4169207959143595 >500 0.001340606728443979 0.68980177973642 >1k 7.660609876816972E-4 0.9974526519483493 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3401 0.15335500714694755 No Hit CCCATGTACTCTGCGTTGATACCAC 3312 0.1493418946400148 No Hit GTATCAACGCAGAGTACTTTTTTTT 3130 0.1411353050190961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.509115176328949E-5 0.0 0.0 4.509115176328949E-5 0.0 2 4.509115176328949E-5 0.0 0.0 4.509115176328949E-5 0.0 3 4.509115176328949E-5 0.0 0.0 4.509115176328949E-5 0.0 4 4.509115176328949E-5 0.0 0.0 4.509115176328949E-5 4.509115176328949E-5 5 4.509115176328949E-5 0.0 0.0 4.509115176328949E-5 4.509115176328949E-5 6 4.509115176328949E-5 0.0 0.0 4.509115176328949E-5 4.509115176328949E-5 7 4.509115176328949E-5 0.0 0.0 4.509115176328949E-5 4.509115176328949E-5 8 9.018230352657898E-5 0.0 0.0 4.509115176328949E-5 4.509115176328949E-5 9 9.018230352657898E-5 0.0 0.0 1.3527345528986846E-4 4.509115176328949E-5 10 9.018230352657898E-5 0.0 0.0 1.8036460705315795E-4 4.509115176328949E-5 11 9.018230352657898E-5 0.0 0.0 1.8036460705315795E-4 4.509115176328949E-5 12 9.018230352657898E-5 0.0 0.0 1.8036460705315795E-4 9.018230352657898E-5 13 1.3527345528986846E-4 0.0 0.0 2.705469105797369E-4 1.3527345528986846E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCC 100 1.464814E-7 12.336106 8 TGCACCG 95 1.0170916E-6 12.022554 5 TAGGACC 425 0.0 11.197478 4 GTCCTAC 1005 0.0 11.180757 1 CCGACCT 60 0.005795418 11.104165 4 GAACCGT 95 1.33444755E-5 11.019429 6 GTGCGCC 140 8.483767E-9 10.84664 11 CTAGTAG 125 2.1806045E-7 10.661204 3 GCGCCAC 125 2.2649692E-7 10.6318655 13 CTAGCAC 230 0.0 10.346665 3 GTCCTAT 295 0.0 10.329594 1 ACCGCAT 75 0.0026731768 10.121933 8 TGGCGAG 760 0.0 9.99258 18 TGTACCG 125 2.5098689E-6 9.89857 5 TCCTACA 1205 0.0 9.796967 2 TATGGCG 800 0.0 9.729836 16 GTATTAA 250 0.0 9.522594 1 CTACACG 110 6.6991524E-5 9.517857 4 TGCGCCA 140 1.0699696E-6 9.492737 12 ACCGCTC 80 0.004558866 9.489312 8 >>END_MODULE