FastQCFastQC Report
Thu 26 May 2016
SRR1513172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144441
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC26460.12338879922553242No Hit
CCCATGTACTCTGCGTTGATACCAC22520.10501571271953856No Hit
GTATCAACGCAGAGTACTTTTTTTT22180.10343021794490966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA4200.011.8264891
AACCGTC600.00593552511.068567
CTTAGAC2100.010.8911853
TCTACAC2900.010.5156273
TAGGACC3450.010.4965764
CTAAGAC2001.8189894E-1210.4827653
GTCCTAC8350.010.4101421
GGCGAGG4100.09.9519919
TTAGGAC5750.09.7783913
CCCTAGA2159.094947E-129.7743071
CCTACAC2355.456968E-129.3270253
CTTGGAC2252.5465852E-119.3180143
GTAAGAC1752.5374902E-89.2575063
ATAATAC1651.0157237E-79.2410053
TCCTACA9500.09.2353362
GTAATAC1554.0722261E-79.2223753
TAGGACG7350.09.2056444
GTAGGAC8400.09.1894373
AGGACCG1252.7199521E-59.1297745
TAGCACT3450.09.1154484
Produced by FastQC (version 0.11.5)