##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513172_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2144441
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.691398364422245	31.0	30.0	34.0	25.0	34.0
2	29.551160418962333	31.0	30.0	34.0	24.0	34.0
3	29.740966060619062	31.0	30.0	34.0	25.0	34.0
4	32.83871227979692	37.0	33.0	37.0	25.0	37.0
5	32.98701199986383	37.0	35.0	37.0	26.0	37.0
6	33.39044207791215	37.0	35.0	37.0	28.0	37.0
7	33.38039936748085	37.0	35.0	37.0	28.0	37.0
8	33.26410519104979	37.0	35.0	37.0	27.0	37.0
9	34.531906916534425	39.0	35.0	39.0	27.0	39.0
10	34.10579820102302	39.0	34.0	39.0	23.0	39.0
11	34.57414822790648	39.0	35.0	39.0	27.0	39.0
12	34.57111713495498	39.0	35.0	39.0	27.0	39.0
13	34.61710767514704	39.0	35.0	39.0	27.0	39.0
14	34.84174197378245	40.0	34.0	41.0	18.0	41.0
15	35.2796337134013	40.0	35.0	41.0	25.0	41.0
16	35.12268745094875	40.0	35.0	41.0	24.0	41.0
17	34.71972509385896	39.0	34.0	41.0	18.0	41.0
18	34.46165317674863	39.0	33.0	41.0	17.0	41.0
19	35.10620483380051	40.0	34.0	41.0	25.0	41.0
20	35.32430456235448	40.0	35.0	41.0	26.0	41.0
21	35.37824449355333	40.0	36.0	41.0	26.0	41.0
22	35.06448253880615	40.0	35.0	41.0	23.0	41.0
23	34.59784018305936	40.0	34.0	41.0	18.0	41.0
24	34.850361003170526	40.0	35.0	41.0	22.0	41.0
25	34.4892454490471	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8363716970939201
1101	2	1.140716476228917
1101	3	1.0164836258514889
1101	4	1.3201229903753884
1101	5	1.1304994349055661
1101	6	0.9179076764751315
1101	7	0.8010842710484596
1101	8	0.9842033772912231
1101	9	1.3535687680914918
1101	10	1.597203427426379
1101	11	1.209761380807123
1101	12	1.004985978958345
1101	13	0.8962031120633611
1101	14	1.9406785586738664
1101	15	1.6854362952410256
1101	16	1.6647392660928517
1101	17	2.0013349396923203
1101	18	2.048534653720111
1101	19	1.6256872203965997
1101	20	1.302542376210063
1101	21	1.2850360770724407
1101	22	1.5880237508878565
1101	23	1.801220893298101
1101	24	1.465625549675643
1101	25	1.79056289638703
1102	1	1.0310561019640048
1102	2	1.2370464139708837
1102	3	1.1799180182938294
1102	4	1.5487708318251947
1102	5	1.4327391134147192
1102	6	1.0704438312711488
1102	7	1.1337807735827283
1102	8	1.1002539592079614
1102	9	1.493297087641622
1102	10	1.7441015616738653
1102	11	1.3063309254744269
1102	12	1.1856035682939492
1102	13	1.2463429987620955
1102	14	2.2226327768493874
1102	15	1.7123160165413864
1102	16	1.8701446392532972
1102	17	2.238636170337351
1102	18	2.4550312823214284
1102	19	1.8015866411642563
1102	20	1.591837017167535
1102	21	1.4245844428736731
1102	22	1.752695747897242
1102	23	1.9439852612627533
1102	24	1.8078426991684893
1102	25	2.081001784690436
1103	1	0.7325480586540252
1103	2	1.0098758430111054
1103	3	0.8301068149784143
1103	4	1.234588330333139
1103	5	1.1446396076149057
1103	6	0.8690380493849972
1103	7	0.7961567557292781
1103	8	0.8588913336635855
1103	9	1.2202317858525404
1103	10	1.5685210389522766
1103	11	1.1238654791086446
1103	12	1.059533966628365
1103	13	1.0528595866731933
1103	14	1.855126980978298
1103	15	1.5152043989959978
1103	16	1.556542414269103
1103	17	1.8528024217235313
1103	18	2.094577539971283
1103	19	1.5083095561771813
1103	20	1.1466044042883894
1103	21	1.1258752921928377
1103	22	1.3142901491941004
1103	23	1.8484972755076896
1103	24	1.5193684560787233
1103	25	1.7758116978459313
1104	1	0.7057089486446628
1104	2	1.092994778869766
1104	3	0.8368885850674239
1104	4	1.2739794675716354
1104	5	1.1836031880657671
1104	6	0.9007113662833603
1104	7	0.8186064195683755
1104	8	0.8489836288439392
1104	9	1.1831177257443457
1104	10	1.4211849112834685
1104	11	1.013124067877996
1104	12	0.8279614295763764
1104	13	0.8568527559305537
1104	14	1.9047771351294287
1104	15	1.499179034927586
1104	16	1.5079356394392391
1104	17	2.0586996556051105
1104	18	2.20234179137546
1104	19	1.4733488221949216
1104	20	1.2367706959223952
1104	21	1.241243583392837
1104	22	1.4985160016195564
1104	23	1.6547679128937887
1104	24	1.4383918006402325
1104	25	1.849134852186701
1105	1	0.833917121484884
1105	2	1.0722832030928835
1105	3	0.9268432125785999
1105	4	1.3089606910842377
1105	5	1.1796214142910628
1105	6	0.9309776159092777
1105	7	0.860427014816814
1105	8	0.9385359857999305
1105	9	1.108013417288653
1105	10	1.547438688301817
1105	11	1.052960437306595
1105	12	0.9113578017080144
1105	13	0.8909996083348091
1105	14	1.7047744320875395
1105	15	1.3388398997079136
1105	16	1.5317936525103732
1105	17	1.8012799021611485
1105	18	1.5764443332002074
1105	19	1.4434717140635982
1105	20	1.2137943571926328
1105	21	1.1404893381690115
1105	22	1.313306371067462
1105	23	1.6766758743175174
1105	24	1.4623385314076032
1105	25	1.8278881706533028
1106	1	0.394337254150841
1106	2	0.5877925532581791
1106	3	0.4643575942443263
1106	4	1.0352427835600793
1106	5	0.8256556922802716
1106	6	0.45084928170721383
1106	7	0.456055083639356
1106	8	0.4456697204156512
1106	9	0.5664928759620409
1106	10	0.60690512076809
1106	11	0.5152674540362128
1106	12	0.396604081039591
1106	13	0.31469649970533453
1106	14	1.3683610779046589
1106	15	0.9175390878608027
1106	16	1.0700006390129104
1106	17	1.3848600741461112
1106	18	1.3930229605097821
1106	19	0.9830459167945023
1106	20	0.6945435128573507
1106	21	0.4641726630530414
1106	22	0.745143439944755
1106	23	1.1760922297880256
1106	24	0.7821075679252516
1106	25	0.9709925159751265
1107	1	0.7172982082729362
1107	2	0.9325193871771198
1107	3	0.9185647779439527
1107	4	1.2079693625428405
1107	5	1.0741887063739455
1107	6	0.816049198693392
1107	7	0.7408935056370112
1107	8	0.8422713724133217
1107	9	1.0588914883808656
1107	10	1.282834781287157
1107	11	0.8909282019472329
1107	12	0.9177068545780784
1107	13	0.8097086772253945
1107	14	1.7222755637140708
1107	15	1.1719554393693485
1107	16	1.2394828829806315
1107	17	1.7723241979339548
1107	18	1.8650739867921402
1107	19	1.4810183951392446
1107	20	1.235622544291381
1107	21	0.9558447636961347
1107	22	1.3099883235794394
1107	23	1.6502653462804346
1107	24	1.415262298882645
1107	25	1.7574030287319289
1108	1	0.6807932694404606
1108	2	0.9957523601755938
1108	3	0.8397461861956366
1108	4	1.235872066558727
1108	5	1.2275022058741385
1108	6	0.7928669977322542
1108	7	0.6657349730335795
1108	8	0.8184552639183664
1108	9	1.0643937426251497
1108	10	1.2792668458272303
1108	11	0.9519216964549173
1108	12	0.6252851049189943
1108	13	0.736768820990342
1108	14	1.6919138445226665
1108	15	1.26914592700453
1108	16	1.326665599537229
1108	17	1.603416535955077
1108	18	1.823975957904679
1108	19	1.2903575637155598
1108	20	1.0764310522108573
1108	21	1.1458702945867287
1108	22	1.4820872540160224
1108	23	1.7960954679765706
1108	24	1.4367621281937986
1108	25	1.8941006472232331
1109	1	0.39367597077966465
1109	2	0.6853187539601713
1109	3	0.6250931700814917
1109	4	0.8985904656050678
1109	5	0.9124794320397172
1109	6	0.6046698809701354
1109	7	0.550010077449393
1109	8	0.6183609675785675
1109	9	0.8370971330959449
1109	10	1.0987204708982645
1109	11	0.7506580488527632
1109	12	0.7112836588106219
1109	13	0.6661385447661274
1109	14	1.6118728227861467
1109	15	1.1916261963121002
1109	16	1.2344237227644967
1109	17	1.5944135805942707
1109	18	1.7371349053626233
1109	19	1.082786171695723
1109	20	0.9607453935398809
1109	21	0.9521501716711711
1109	22	1.1813908809302873
1109	23	1.4477623797979007
1109	24	1.280102182293291
1109	25	1.5413292254198439
1110	1	0.49441434362955405
1110	2	0.7916193096285191
1110	3	0.5965256902208189
1110	4	0.9693052940417033
1110	5	0.7342554539534376
1110	6	0.3897463326983228
1110	7	0.47950701891146963
1110	8	0.4524502957099372
1110	9	0.6665333834146381
1110	10	1.000329146810806
1110	11	0.5828367599286608
1110	12	0.5726145834904912
1110	13	0.39122416327306553
1110	14	1.2746394013310507
1110	15	0.9414348911928769
1110	16	0.9771398840262364
1110	17	1.3605639878884617
1110	18	1.0196075519760868
1110	19	0.7143119571375678
1110	20	0.4895446573126776
1110	21	0.3269418726711848
1110	22	0.7381242982383327
1110	23	-0.5619561366263923
1110	24	0.08153486110226993
1110	25	0.6414641126580136
1111	1	0.1396880086511203
1111	2	0.5346914718267328
1111	3	0.44962701964147556
1111	4	0.8406747307052598
1111	5	0.6308255486579881
1111	6	0.29921189670169923
1111	7	0.32402046873584567
1111	8	0.36350067141577824
1111	9	0.5691813759449857
1111	10	0.9390528140554792
1111	11	0.3915168541885876
1111	12	0.3234232625176574
1111	13	0.1652321664743468
1111	14	1.1183971711056273
1111	15	0.7551499772272905
1111	16	0.8422846085827231
1111	17	1.1801326153394385
1111	18	1.3737914477566235
1111	19	0.8378696468801294
1111	20	0.49438694978522335
1111	21	0.3642068230957989
1111	22	0.7159730377341305
1111	23	1.110619026772298
1111	24	0.7474789276177702
1111	25	0.9901069475697497
1112	1	0.15312357390108033
1112	2	0.40278691765923824
1112	3	0.18932420165394603
1112	4	0.6382810937607601
1112	5	0.3811691272157276
1112	6	0.1991440358604919
1112	7	0.025596602871168272
1112	8	0.22693980596972807
1112	9	0.3601481285393362
1112	10	0.655163418413558
1112	11	0.14388057067128557
1112	12	0.19596570859252438
1112	13	0.09946337805475736
1112	14	0.6683037082197814
1112	15	0.5188089104019866
1112	16	0.38612888117179267
1112	17	0.8977879792101504
1112	18	0.8909681723693978
1112	19	0.45211487128359806
1112	20	0.3139728223919178
1112	21	0.24330586026503198
1112	22	0.518807065098791
1112	23	0.8055247046095673
1112	24	0.5653819394934132
1112	25	0.9099026599984228
1113	1	-0.18329176419428705
1113	2	-0.031724240989696284
1113	3	-0.13660055520120906
1113	4	0.21454907683400393
1113	5	0.2821573558309396
1113	6	-0.11698884868387438
1113	7	0.00643250992006017
1113	8	-0.048035685484570934
1113	9	0.0918629060866536
1113	10	0.3917633854808926
1113	11	-0.0074399598963239555
1113	12	-0.23734848803253072
1113	13	-0.31468104144841647
1113	14	0.36192406138796684
1113	15	-0.04513587924727602
1113	16	0.1611287079822148
1113	17	0.5239709750488046
1113	18	0.28436066134165827
1113	19	0.1920604169275748
1113	20	-0.02699393782080506
1113	21	-0.14458328561548228
1113	22	-0.012824812370382688
1113	23	0.08241531753903075
1113	24	-0.059155932981859394
1113	25	0.2942892020846415
1114	1	-0.8328065447257877
1114	2	-0.4394131033366655
1114	3	-0.5278740696831434
1114	4	-0.2253165546977911
1114	5	-3.4143639938256705
1114	6	-1.2294373407710637
1114	7	-0.9297006641617571
1114	8	-0.5438762349023065
1114	9	-0.288256532551749
1114	10	-0.15889054106434486
1114	11	-0.5807946926523684
1114	12	-0.6574634477219945
1114	13	-0.568277637447224
1114	14	-6.210870802750581
1114	15	-2.3941833561060406
1114	16	-1.340297090763677
1114	17	-0.4106124826997757
1114	18	-6.53785343241767
1114	19	-2.5883591112898046
1114	20	-1.4873821133956042
1114	21	-1.245573133331213
1114	22	-0.8320857857952859
1114	23	-0.4290149601558042
1114	24	-0.5451398087656756
1114	25	-0.4402285644128199
1115	1	-0.7085667359354559
1115	2	-0.32958041179503894
1115	3	-0.3608145822312778
1115	4	-3.1139482105203946
1115	5	-1.0374917325057922
1115	6	-0.6608428646058826
1115	7	-0.5583402870538023
1115	8	-0.4566096036448357
1115	9	-0.3044057823193782
1115	10	-0.6183202316764849
1115	11	-0.6408783468815571
1115	12	-0.5708600749959274
1115	13	-0.6540424761140642
1115	14	-5.82833395505439
1115	15	-2.3863503989954324
1115	16	-1.2449777440633767
1115	17	-0.30825489406613116
1115	18	-0.06969780899448352
1115	19	-0.4531015093777029
1115	20	-0.3695577872768965
1115	21	-0.7356528040256762
1115	22	-1.8355687229882207
1115	23	-0.9502013895808474
1115	24	-0.6532741866703375
1115	25	-0.29222697099892514
1116	1	-0.2436580690305874
1116	2	-0.02106383668452949
1116	3	-0.10244278017422204
1116	4	-0.02013267278081088
1116	5	-0.5365403709937979
1116	6	-0.34258995809115333
1116	7	-0.32226535758809405
1116	8	-0.23929273289377306
1116	9	-0.255100489827619
1116	10	-0.11665904603118094
1116	11	-0.43268940818813206
1116	12	-0.8264229033941461
1116	13	-0.662042934990275
1116	14	0.2061793015685467
1116	15	-0.172076421219856
1116	16	-0.2060313728301324
1116	17	0.11886280768106872
1116	18	0.12996069270300126
1116	19	-0.08004082897422649
1116	20	-0.5357556692763978
1116	21	-0.6748243711476789
1116	22	-0.5894711126099708
1116	23	-1.2878615331947572
1116	24	-0.9201991305356714
1116	25	-0.379387160619963
1201	1	0.8523519473773788
1201	2	1.021127732449962
1201	3	1.0450281318017964
1201	4	1.3507450276310777
1201	5	1.2647015322903457
1201	6	1.0394658050009369
1201	7	0.7636561837709976
1201	8	0.9248117615427347
1201	9	1.2295427231278637
1201	10	1.5121779845517551
1201	11	1.0209802892950819
1201	12	0.9589905692011129
1201	13	0.9618302149477458
1201	14	1.86122625514718
1201	15	1.47332687367048
1201	16	1.6188536989195228
1201	17	2.011830443479191
1201	18	1.4049319366874826
1201	19	1.3617626276796315
1201	20	1.3730011560594875
1201	21	1.218281316853016
1201	22	1.502088240755846
1201	23	1.7421783594761067
1201	24	1.5280509557365392
1201	25	1.7962116585129877
1202	1	0.9356335008766976
1202	2	1.0347874740496508
1202	3	0.9844022850600673
1202	4	1.396575764593159
1202	5	1.208561368879586
1202	6	0.9023966882453536
1202	7	0.9127385172000686
1202	8	1.0612979923378276
1202	9	1.189123163309091
1202	10	1.487402969775438
1202	11	1.10206677140809
1202	12	1.0139716514818033
1202	13	1.024366479280431
1202	14	1.890087768861875
1202	15	1.5367200512087464
1202	16	1.6333054136954317
1202	17	2.0043635208307364
1202	18	2.085279991630287
1202	19	1.6532030496796182
1202	20	1.3990397463767508
1202	21	1.3293750122289794
1202	22	1.4917329627106852
1202	23	1.81023737972982
1202	24	1.4061229020079153
1202	25	1.8439834135950122
1203	1	0.5970799696575391
1203	2	0.7711186163020685
1203	3	0.6996673175853871
1203	4	0.9640619548153708
1203	5	0.9718441873151846
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2211	16	-7.300267810106394
2211	17	-2.4855989349329732
2211	18	-1.0903877388594125
2211	19	-1.142198373592045
2211	20	-1.2646603233422837
2211	21	-1.1691956313646017
2211	22	-0.9715277052079188
2211	23	-0.5699449487931574
2211	24	-0.9681937376180514
2211	25	-8.422179442832537
2212	1	-1.5329823293253426
2212	2	-4.065163403456715
2212	3	-1.7944278340460293
2212	4	-3.8235861482495004
2212	5	-1.290054269936114
2212	6	-1.1134692392447434
2212	7	-0.9847358210816708
2212	8	-0.7416244770563054
2212	9	-0.8638902145959904
2212	10	-0.3200189924449006
2212	11	-0.8148651779893612
2212	12	-0.8001430910078753
2212	13	-0.9697688053429516
2212	14	-6.63242628208517
2212	15	-5.0929588663101235
2212	16	-2.6477186308082707
2212	17	-7.458052029947247
2212	18	-2.987638203468947
2212	19	-1.7672006479044455
2212	20	-1.8252726982009548
2212	21	-1.3828934900453262
2212	22	-1.213496350020975
2212	23	-0.7265419709601275
2212	24	-1.0904768007053107
2212	25	-0.8157559182114795
2213	1	-0.1451211660057723
2213	2	-0.14529047966790998
2213	3	-0.17830454644237292
2213	4	0.3117256801318433
2213	5	0.07221077144422594
2213	6	-0.13336189536448018
2213	7	-0.15076200917353333
2213	8	-0.19539310560998047
2213	9	-0.057534924111415364
2213	10	-3.9265903519531697
2213	11	-1.444883973034571
2213	12	-0.8472652127596447
2213	13	-0.6647689615572361
2213	14	0.08474347680218841
2213	15	-0.46642249880203934
2213	16	-0.2539703265554607
2213	17	0.1923131003930223
2213	18	0.3451582460269762
2213	19	-0.3054512781057639
2213	20	-0.6653934600662552
2213	21	-0.6207121466353556
2213	22	-0.8379965054130807
2213	23	-1.030985034141402
2213	24	-0.5533167106601056
2213	25	-0.29661687269721426
2214	1	-1.2326122365640018
2214	2	-0.9314814526024264
2214	3	-0.9737312173573471
2214	4	-0.5436098462190486
2214	5	-3.9578527736884332
2214	6	-1.8045249269663373
2214	7	-1.5109042788836575
2214	8	-1.218296850956122
2214	9	-1.2277682753400043
2214	10	-4.524035113080597
2214	11	-2.2031859896698833
2214	12	-1.5746872435171824
2214	13	-1.3281630757153948
2214	14	-0.6411014435881697
2214	15	-0.7680555257821666
2214	16	-0.8259718163718972
2214	17	-0.4652878489769847
2214	18	-0.06559583290322735
2214	19	-0.945681704987642
2214	20	-1.2342996525522807
2214	21	-1.194219214291543
2214	22	-0.9371034884911538
2214	23	-0.787370464008248
2214	24	-1.4755307854486972
2214	25	-0.9251930747225288
2215	1	-2.313444212946532
2215	2	-5.001743229316233
2215	3	-3.0448325394116758
2215	4	-1.9705875264929205
2215	5	-1.9927937130995517
2215	6	-2.1594003074079744
2215	7	-2.3863593951077604
2215	8	-2.558375788518017
2215	9	-8.35644950282586
2215	10	-4.019354780723109
2215	11	-3.296872628042376
2215	12	-3.459541449243005
2215	13	-2.908469913345776
2215	14	-8.1598662727767
2215	15	-4.799377285279736
2215	16	-9.529467311971892
2215	17	-4.695242314396367
2215	18	-3.0928689732039665
2215	19	-3.6228670966087186
2215	20	-3.4831702296539113
2215	21	-3.597356851877656
2215	22	-9.571449109539621
2215	23	-10.784156315180105
2215	24	-6.839855280114417
2215	25	-5.225888055865386
2216	1	-1.8885404323297124
2216	2	-1.8917135346675416
2216	3	-1.687896261344079
2216	4	-7.226764951272152
2216	5	-3.637768382148483
2216	6	-2.8077007651631405
2216	7	-2.2576008954551234
2216	8	-8.294519480510857
2216	9	-4.094138260375715
2216	10	-2.360573981709468
2216	11	-2.2802073950340684
2216	12	-2.5574668858666456
2216	13	-2.291123483543366
2216	14	-1.5387529056448699
2216	15	-2.1389867567436625
2216	16	-1.7993472251638778
2216	17	-1.505504649981141
2216	18	-7.8628027979237025
2216	19	-5.011961974326621
2216	20	-4.381157821340842
2216	21	-3.4697779842407606
2216	22	-2.991514741224428
2216	23	-2.2919836417836095
2216	24	-2.760231388436864
2216	25	-2.205969015747442
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81732.0
3	3.0
4	684.0
5	3999.0
6	4961.0
7	4701.0
8	4357.0
9	4045.0
10	3839.0
11	3755.0
12	3723.0
13	3760.0
14	3893.0
15	3877.0
16	3933.0
17	4265.0
18	4713.0
19	5718.0
20	7584.0
21	9795.0
22	12023.0
23	14510.0
24	17055.0
25	19501.0
26	22217.0
27	25212.0
28	28076.0
29	33859.0
30	41245.0
31	49155.0
32	62775.0
33	81986.0
34	108142.0
35	145978.0
36	207866.0
37	302987.0
38	808517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.058247892192355	18.092813602073942	12.17857393091529	25.67036457481841
2	14.899926737460628	20.20610758045204	40.45560229334658	24.438363388740758
3	18.46613702288577	24.70403909489264	29.627276280673797	27.20254760154779
4	11.683002899620833	16.64426398402936	38.60196953078991	33.070763585559895
5	13.101385013577097	37.675604610976876	35.81473807048122	13.408272304964806
6	32.09951688387769	36.68310324916348	18.16107104577523	13.0563088211836
7	28.129607302187722	31.38714048175969	22.37344421564215	18.109808000410446
8	25.667782007595015	35.46285093827628	20.833679253819195	18.03568780030951
9	26.845796604746376	14.771270242020378	19.812331476828515	38.57060167640473
10	14.185491458004595	28.336653549054848	35.26852701362027	22.209327979320285
11	34.617266468248694	22.28899899671589	24.107783777581567	18.98595075745385
12	23.172106635295478	24.591413443368833	31.26561193174901	20.97086798958668
13	28.926018060394433	21.440222930515667	25.524219297725963	24.109539711363944
14	21.103062247825175	20.925764395829933	28.4053455232409	29.565827833103995
15	23.851837130569017	28.937879659422084	23.469804290393476	23.74047891961542
16	23.000826517209468	27.388177632168127	26.73615257171856	22.874843278903846
17	22.207863700828543	27.401657087407628	27.717539374486826	22.672939837277003
18	22.151627997669458	27.035551033793332	28.61836995447605	22.194451014061155
19	23.65272100609146	26.79519418832076	27.128337673714363	22.423747131873416
20	24.039668527100684	26.670600555564626	27.030434772468066	22.259296144866624
21	23.9197072290084	26.345237456586997	26.59765680578265	23.137398508621956
22	23.700648433640172	26.68580383331164	26.651840261108955	22.96170747193923
23	23.096952474423585	26.592286866327203	27.05791474093327	23.25284591831594
24	23.413657803759467	26.669528177326573	26.942833548724217	22.973980470189744
25	23.357171847228248	26.362364071487555	26.9792730886037	23.301190992680503
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	280.0
1	280.0
2	411.5
3	543.0
4	543.0
5	543.0
6	1018.0
7	1493.0
8	1493.0
9	1493.0
10	2225.5
11	2958.0
12	2958.0
13	2958.0
14	5547.0
15	8136.0
16	8136.0
17	8136.0
18	15150.0
19	22164.0
20	22164.0
21	22164.0
22	34758.0
23	47352.0
24	47352.0
25	47352.0
26	67708.5
27	88065.0
28	88065.0
29	88065.0
30	113435.0
31	138805.0
32	138805.0
33	138805.0
34	162665.5
35	186526.0
36	186526.0
37	186526.0
38	207655.0
39	228784.0
40	228784.0
41	228784.0
42	245315.5
43	261847.0
44	261847.0
45	261847.0
46	274969.5
47	288092.0
48	288092.0
49	288092.0
50	281290.5
51	274489.0
52	274489.0
53	274489.0
54	249081.5
55	223674.0
56	223674.0
57	223674.0
58	193169.0
59	162664.0
60	162664.0
61	162664.0
62	133747.0
63	104830.0
64	104830.0
65	104830.0
66	81450.5
67	58071.0
68	58071.0
69	58071.0
70	42850.0
71	27629.0
72	27629.0
73	27629.0
74	19479.5
75	11330.0
76	11330.0
77	11330.0
78	7867.0
79	4404.0
80	4404.0
81	4404.0
82	2992.0
83	1580.0
84	1580.0
85	1580.0
86	1053.5
87	527.0
88	527.0
89	527.0
90	339.5
91	152.0
92	152.0
93	152.0
94	93.0
95	34.0
96	34.0
97	34.0
98	23.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16745622752036546
2	0.25918176345257343
3	0.2632387647876533
4	0.4674411653200065
5	0.25857554486227413
6	0.020658064269429656
7	0.019865316882115198
8	0.018792776299277995
9	0.020891225265698612
10	0.02350262842391094
11	0.022476720040327525
12	0.030217665116456925
13	0.029564814326903843
14	0.027559629758990803
15	0.042342036922442726
16	0.02354926062316473
17	0.04294825551274202
18	0.034228034252283
19	0.02891196353735076
20	0.03152336669556308
21	0.03665290861348016
22	0.04513996887767022
23	0.031196941300786547
24	0.049010441415734914
25	0.039684001564976604
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2144441.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.39134737125815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.3727495002405	59.630743229975415
2	11.882594928259582	17.203941142471795
3	3.0207186617190795	6.560216818540438
4	1.1332415803203761	3.2814753958610354
5	0.523515419139489	1.8948993280568278
6	0.2796725404492236	1.2147523215517193
7	0.17521056666418983	0.8878610296151643
8	0.12164447827955013	0.7044806146344303
9	0.08481438094801544	0.5525844581957404
>10	0.37694463412326285	4.672379994278848
>50	0.017934169517940173	0.8742035450739705
>100	0.009069269505561148	1.2305011681168532
>500	0.0013033591953122681	0.629751945153766
>1k	5.865116378902048E-4	0.6622090084740221
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2646	0.12338879922553242	No Hit
CCCATGTACTCTGCGTTGATACCAC	2252	0.10501571271953856	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2218	0.10343021794490966	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.663219925379155E-5	0.0
4	0.0	0.0	0.0	1.3989659776137464E-4	0.0
5	0.0	0.0	0.0	1.3989659776137464E-4	0.0
6	0.0	0.0	0.0	1.865287970151662E-4	0.0
7	0.0	0.0	0.0	1.865287970151662E-4	0.0
8	0.0	0.0	0.0	1.865287970151662E-4	0.0
9	0.0	0.0	0.0	1.865287970151662E-4	4.663219925379155E-5
10	0.0	0.0	0.0	3.264253947765408E-4	9.32643985075831E-5
11	0.0	0.0	0.0	3.264253947765408E-4	9.32643985075831E-5
12	0.0	0.0	0.0	3.264253947765408E-4	1.3989659776137464E-4
13	0.0	0.0	0.0	3.264253947765408E-4	1.3989659776137464E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	420	0.0	11.826489	1
AACCGTC	60	0.005935525	11.06856	7
CTTAGAC	210	0.0	10.891185	3
TCTACAC	290	0.0	10.515627	3
TAGGACC	345	0.0	10.496576	4
CTAAGAC	200	1.8189894E-12	10.482765	3
GTCCTAC	835	0.0	10.410142	1
GGCGAGG	410	0.0	9.95199	19
TTAGGAC	575	0.0	9.778391	3
CCCTAGA	215	9.094947E-12	9.774307	1
CCTACAC	235	5.456968E-12	9.327025	3
CTTGGAC	225	2.5465852E-11	9.318014	3
GTAAGAC	175	2.5374902E-8	9.257506	3
ATAATAC	165	1.0157237E-7	9.241005	3
TCCTACA	950	0.0	9.235336	2
GTAATAC	155	4.0722261E-7	9.222375	3
TAGGACG	735	0.0	9.205644	4
GTAGGAC	840	0.0	9.189437	3
AGGACCG	125	2.7199521E-5	9.129774	5
TAGCACT	345	0.0	9.115448	4
>>END_MODULE