FastQCFastQC Report
Thu 26 May 2016
SRR1513172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144441
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31950.148989876615864No Hit
GTACATGGGAAGCAGTGGTATCAAC29610.1380779419904768No Hit
CCCATGTACTCTGCGTTGATACCAC28990.1351867456367417No Hit
TATCAACGCAGAGTACTTTTTTTTT22440.10464265512550824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC7000.011.4242263
GTCCTAA4300.011.2958791
TAGGACC2950.011.2946114
ACCGTCC855.379701E-511.1652228
TAGGACG8000.010.9477044
CTAAGAC3050.010.9248073
CTGTCGA700.001506435710.8444459
AGGACGT7600.010.7723415
GGACGTG7250.010.7656476
TGTAGGA8450.010.7036722
CCTATAC1252.1764936E-710.6626123
ACACCGT909.333023E-510.5760096
TCCAACG909.608856E-510.5456718
GTACCGT1002.3488152E-510.4702486
TCTACAC4100.010.4489873
GTCTTAT1553.5142875E-910.4456511
TGGCGAG8400.010.16903918
TGGACCG750.002603048510.1543925
GCCTTAT2450.010.1070771
GCGTGCG856.666442E-410.047069
Produced by FastQC (version 0.11.5)