FastQCFastQC Report
Thu 26 May 2016
SRR1513171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3979
Sequences flagged as poor quality0
Sequence length25
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCG250.6282985674792662No Hit
TCTCCGAGCCCACGAGACGTAGAGG240.6031666247800955No Hit
TCTCCGAGCCCACGAGACGGACTCC190.4775069112842423No Hit
CTCCGAGCCCACGAGACGTAGAGGA90.2261874842925358No Hit
TCTCCGAGCCCACGAGACTCCTGAG80.20105554159336514No Hit
CTCCGAGCCCACGAGACGGACTCCT80.20105554159336514No Hit
TCTCCGAGCCCACGAGACAGGCAGA80.20105554159336514No Hit
CTCCGAGCCCACGAGACTAAGGCGA40.10052777079668257No Hit
CTCCGAGCCCACGAGACAGGCAGAA40.10052777079668257No Hit
TCTCCGAGCCCACGAGACTAGGCAT40.10052777079668257No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)