FastQCFastQC Report
Thu 26 May 2016
SRR1513170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558873
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20670.13259579195996082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC1001.0229996E-813.28519513
GTACTAG1001.8103401E-611.4605021
CTAGCAC1407.968993E-910.889443
GTAGGAC11250.010.8410433
TAGGACC4850.010.8048954
GTATTAA2050.010.7151041
TCCAACG803.8062982E-410.67663218
GTAGGGG1451.3773388E-810.5139433
GTCCTAC10500.010.4599821
GTGCGCC1002.4271794E-510.43870311
GGCGAGG4100.010.41622519
TGGCGAG8750.010.41225718
TATGGCG8500.010.38288116
GTATAAG1303.5707126E-710.2850661
GGCGAGA5050.010.14808619
TGTACCG750.002623455610.1445185
ATTATCC1701.4788384E-910.0887463
TAGAGTT1701.5279511E-910.0699265
TAGGACT2950.010.0124254
GGACGTG8200.09.95008856
Produced by FastQC (version 0.11.5)