FastQCFastQC Report
Thu 26 May 2016
SRR1513170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558873
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30680.19680884844371543No Hit
TATCAACGCAGAGTACTTTTTTTTT21650.1388823849024263No Hit
GGTATCAACGCAGAGTACTTTTTTT18780.1204716484280631No Hit
CCCATGTACTCTGCGTTGATACCAC16750.10744942019009888No Hit
GTACATGGGAAGCAGTGGTATCAAC16480.10571739968554204No Hit
GTCCTACAGTGGACATTTCTAAATT16110.10334389010522346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACCG550.003022711512.1151765
TAGGACC5200.011.7157754
GTCCTAC9450.010.9835531
GACAGTC1658.185452E-1110.9303967
AGGACCT8100.010.6942615
GCGCCAC803.7994626E-410.67870913
GGCGAGG5250.010.66330319
TAGGGGT1252.1783671E-710.6613544
GTGCTAG1701.3278623E-1010.6427241
TCGAACT1002.430565E-510.43738619
TGGCGAG10150.010.28643818
TTAGGAC7050.010.262353
GCCTCGA1303.8835242E-710.22235516
GTCTTAT1502.3554094E-810.1572671
TGGGCCG750.002603897410.1536715
ACACCGT750.002605983810.1526926
GTAGGAC11450.010.1432393
TGCGTTG5250.010.12499812
GACGTGA6150.010.03239257
CTCTAAT1053.9991843E-59.9758881
Produced by FastQC (version 0.11.5)