##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513170_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1558873 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.800813151552436 31.0 31.0 34.0 27.0 34.0 2 30.917338359186413 33.0 31.0 34.0 27.0 34.0 3 31.012399983834474 33.0 31.0 34.0 27.0 34.0 4 34.39228789003338 37.0 35.0 37.0 31.0 37.0 5 34.1466482516536 37.0 35.0 37.0 30.0 37.0 6 34.054761997930555 37.0 35.0 37.0 30.0 37.0 7 33.926360903037 37.0 35.0 37.0 30.0 37.0 8 33.88803000629301 37.0 35.0 37.0 29.0 37.0 9 35.45454568781421 39.0 37.0 39.0 30.0 39.0 10 35.296859975123056 39.0 35.0 39.0 29.0 39.0 11 35.31191828968748 39.0 35.0 39.0 29.0 39.0 12 35.25309630739643 39.0 35.0 39.0 29.0 39.0 13 35.22294054743395 39.0 35.0 39.0 29.0 39.0 14 36.39526696530121 40.0 37.0 41.0 28.0 41.0 15 36.370894870845795 40.0 37.0 41.0 28.0 41.0 16 36.333375457782644 40.0 37.0 41.0 28.0 41.0 17 36.279326795704335 40.0 37.0 41.0 28.0 41.0 18 36.240589194886304 40.0 37.0 41.0 27.0 41.0 19 36.316491465308594 40.0 37.0 41.0 28.0 41.0 20 36.28577760985019 40.0 37.0 41.0 28.0 41.0 21 36.23489277189354 40.0 37.0 41.0 27.0 41.0 22 36.175333718654436 40.0 37.0 41.0 27.0 41.0 23 36.099314697220365 40.0 37.0 41.0 27.0 41.0 24 36.03867345191045 40.0 37.0 41.0 27.0 41.0 25 35.988789336911985 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3874716128387128 1101 2 0.42846352883260863 1101 3 0.38622822258091105 1101 4 0.4533340815376121 1101 5 0.38630380443717627 1101 6 0.3966936522671105 1101 7 0.377175842687123 1101 8 0.43072505729003296 1101 9 0.495950254315602 1101 10 0.6657109951364646 1101 11 0.5417951107168406 1101 12 0.4829084784409048 1101 13 0.5248579387397925 1101 14 0.6050294034366388 1101 15 0.649763146458362 1101 16 0.6190479370678972 1101 17 0.5437743292519954 1101 18 0.6579938544002175 1101 19 0.609247184247053 1101 20 0.6443733647768468 1101 21 0.6434465255795061 1101 22 0.6633809933503585 1101 23 0.632045210615793 1101 24 0.6409690825312921 1101 25 0.6424545244844992 1102 1 0.5044410619163777 1102 2 0.5584581106210429 1102 3 0.44348809488110064 1102 4 0.553945843660415 1102 5 0.6441881875932722 1102 6 0.5387977598380829 1102 7 0.5658294702328845 1102 8 0.6665941490490397 1102 9 0.6948846727077722 1102 10 0.7547739986671544 1102 11 0.6699969019138408 1102 12 0.8003221854537728 1102 13 0.8058998643651023 1102 14 0.8568105076894028 1102 15 0.8375912935362493 1102 16 0.9143516225141539 1102 17 1.014490489333589 1102 18 0.9971085611262325 1102 19 0.9866327023945161 1102 20 0.9228770530561548 1102 21 0.9839715609041235 1102 22 0.9922248249112613 1102 23 0.9987715484359754 1102 24 1.0054990969444475 1102 25 1.112736873509398 1103 1 0.4439492178888287 1103 2 0.44468877643598503 1103 3 0.4714128901242738 1103 4 0.675840947938724 1103 5 0.571053405794153 1103 6 0.387095514083569 1103 7 0.5118885973580731 1103 8 0.6086040222793301 1103 9 0.5977879298402939 1103 10 0.5442450476154619 1103 11 0.6122426920707227 1103 12 0.4738619817348777 1103 13 0.6413855832836219 1103 14 0.6780599586569025 1103 15 0.598684957367098 1103 16 0.5244858938132779 1103 17 0.6615789020527885 1103 18 0.6490177687464964 1103 19 0.6821644464959249 1103 20 0.6671828398809794 1103 21 0.6317593497085809 1103 22 0.7459170406326976 1103 23 0.6123602921337934 1103 24 0.7069230622910254 1103 25 0.7722162704244795 1104 1 0.24188539069585246 1104 2 0.29597791973806764 1104 3 0.3527380134982714 1104 4 0.41729942432600353 1104 5 0.2972417428602583 1104 6 0.28567748853814123 1104 7 0.28600722463918515 1104 8 0.3466179736484776 1104 9 0.34978159375391016 1104 10 0.498882776207644 1104 11 0.3523176897711622 1104 12 0.33039251452657936 1104 13 0.3977141827347239 1104 14 0.3518493746973448 1104 15 0.36814991957947285 1104 16 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-1.4919504360371292 2216 1 -0.5533722354518495 2216 2 -0.564154341081224 2216 3 -0.6651690458931228 2216 4 -0.3320222980615384 2216 5 -0.5302158366102745 2216 6 -0.7443636457170655 2216 7 -0.9751342973547779 2216 8 -1.042172345082328 2216 9 -1.3031451443076136 2216 10 -1.1091284581382652 2216 11 -1.0785086022051331 2216 12 -1.2016880937617955 2216 13 -1.2486346548446363 2216 14 -1.3637388106746684 2216 15 -1.1671072557092117 2216 16 -1.3048052631022315 2216 17 -1.4049780533506677 2216 18 -1.4210750796115335 2216 19 -1.3019690326787483 2216 20 -1.2347230052656357 2216 21 -1.5450508200829773 2216 22 -1.4747777995693099 2216 23 -1.491940706430249 2216 24 -1.536498059868272 2216 25 -1.773889210911392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37216.0 3 0.0 4 634.0 5 5289.0 6 5948.0 7 4556.0 8 3575.0 9 3259.0 10 2918.0 11 2649.0 12 2333.0 13 2248.0 14 2208.0 15 2170.0 16 2204.0 17 2309.0 18 2763.0 19 3171.0 20 4005.0 21 5159.0 22 6668.0 23 7983.0 24 9106.0 25 10334.0 26 11229.0 27 12532.0 28 13968.0 29 16790.0 30 21010.0 31 27273.0 32 35081.0 33 46006.0 34 61366.0 35 82548.0 36 118979.0 37 196775.0 38 786611.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.762947101072996 19.544171555194524 13.460910628627836 27.23197071510465 2 13.669697519866919 20.916928789598312 39.95638804757924 25.456985642955527 3 16.753143776534937 25.24907075598187 30.306121270674634 27.691664196808556 4 11.024194852082697 17.643587171006207 38.306527144473726 33.02569083243737 5 12.426274826773529 38.366878675023735 36.41848613578336 12.788360362419374 6 31.124581092239833 38.245964744491204 18.141803374174494 12.487650789094463 7 27.152506134433942 31.913045040605333 22.996747980000208 17.937700844960524 8 24.756854555428305 36.89779839410767 20.828673807530308 17.51667324293372 9 26.11145432582046 14.802754185776026 20.06863011141327 39.017161376990245 10 13.95954847434315 29.314397448300106 35.365382690832206 21.36067138652454 11 33.88755851182233 22.5201154936932 24.96630578629561 18.626020208188866 12 22.526480686034517 25.206046423311214 32.18891608804315 20.078556802611118 13 28.565251948041954 21.968755633075947 26.03393605508595 23.432056363796153 14 20.687712977788724 21.95403272159954 28.102868271954673 29.25538602865706 15 22.94163796537627 30.468036844566555 23.647596693252112 22.942728496805064 16 22.159662782022654 28.26400867806422 27.49941784866375 22.07691069124938 17 21.57675203760368 28.485452379134475 27.874893091171337 22.06290249209051 18 21.524045899245216 27.7349326930419 29.23770113644457 21.50332027126831 19 22.957290298824855 27.31171814509585 27.854610349913045 21.876381206166247 20 23.240315279050954 27.287983049292663 28.09510460441614 21.376597067240244 21 22.946339736076872 26.863104711575037 27.49728488006964 22.693270672278448 22 22.840519093959085 27.824129016531206 27.32953998729849 22.005811902211217 23 22.152887816832287 27.59390296319581 27.68410112972241 22.569108090249486 24 22.65790734716683 27.217098506420985 28.097029071643426 22.02796507476876 25 22.47264530208683 27.22813211852409 27.844923864869042 22.45429871452004 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 170.0 2 270.0 3 370.0 4 370.0 5 370.0 6 879.5 7 1389.0 8 1389.0 9 1389.0 10 2028.5 11 2668.0 12 2668.0 13 2668.0 14 4822.0 15 6976.0 16 6976.0 17 6976.0 18 13144.0 19 19312.0 20 19312.0 21 19312.0 22 30462.0 23 41612.0 24 41612.0 25 41612.0 26 58870.5 27 76129.0 28 76129.0 29 76129.0 30 96947.5 31 117766.0 32 117766.0 33 117766.0 34 134705.0 35 151644.0 36 151644.0 37 151644.0 38 164609.5 39 177575.0 40 177575.0 41 177575.0 42 185476.5 43 193378.0 44 193378.0 45 193378.0 46 199459.5 47 205541.0 48 205541.0 49 205541.0 50 197593.5 51 189646.0 52 189646.0 53 189646.0 54 168233.5 55 146821.0 56 146821.0 57 146821.0 58 125373.0 59 103925.0 60 103925.0 61 103925.0 62 84194.0 63 64463.0 64 64463.0 65 64463.0 66 49551.5 67 34640.0 68 34640.0 69 34640.0 70 25046.5 71 15453.0 72 15453.0 73 15453.0 74 10730.0 75 6007.0 76 6007.0 77 6007.0 78 4155.5 79 2304.0 80 2304.0 81 2304.0 82 1540.0 83 776.0 84 776.0 85 776.0 86 504.0 87 232.0 88 232.0 89 232.0 90 151.5 91 71.0 92 71.0 93 71.0 94 38.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18276023768453237 2 0.008082762354598483 3 0.005067763698518096 4 0.0018603183197091744 5 0.07890315631869947 6 0.3290838958657954 7 0.029316050762313542 8 0.015267440003130465 9 0.005260210421246632 10 0.004426274622756312 11 0.0 12 6.414890757617843E-5 13 0.0 14 0.0016037226894044607 15 0.0 16 0.0 17 0.019372970088005887 18 0.026685945551690227 19 0.0 20 0.0 21 6.414890757617843E-4 22 1.9244672272853528E-4 23 0.00532435932882281 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1558873.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.57979129172494 #Duplication Level Percentage of deduplicated Percentage of total 1 84.5962742650283 63.93768753008362 2 10.946249850102454 16.546305581956382 3 2.5283446739166555 5.732752883044957 4 0.8335228208156218 2.519899233365382 5 0.35929069849202533 1.3577558002542676 6 0.2024329398041614 0.9179903604581303 7 0.11217184846440961 0.5934547427022955 8 0.08058009969849084 0.4872181693982661 9 0.05485342565731279 0.37312294165342663 >10 0.25869394071220914 3.3735902663924886 >50 0.014760559032694201 0.7751507702911146 >100 0.01087161545845312 1.6874873371461052 >500 0.0010190936437666999 0.5043715246372623 >1k 9.341691734528082E-4 1.1932128586162771 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3068 0.19680884844371543 No Hit TATCAACGCAGAGTACTTTTTTTTT 2165 0.1388823849024263 No Hit GGTATCAACGCAGAGTACTTTTTTT 1878 0.1204716484280631 No Hit CCCATGTACTCTGCGTTGATACCAC 1675 0.10744942019009888 No Hit GTACATGGGAAGCAGTGGTATCAAC 1648 0.10571739968554204 No Hit GTCCTACAGTGGACATTTCTAAATT 1611 0.10334389010522346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.414890757617843E-5 7 0.0 0.0 0.0 0.0 6.414890757617843E-5 8 6.414890757617843E-5 0.0 0.0 0.0 6.414890757617843E-5 9 6.414890757617843E-5 0.0 0.0 6.414890757617843E-5 6.414890757617843E-5 10 6.414890757617843E-5 0.0 0.0 6.414890757617843E-5 6.414890757617843E-5 11 6.414890757617843E-5 0.0 0.0 6.414890757617843E-5 6.414890757617843E-5 12 6.414890757617843E-5 0.0 0.0 6.414890757617843E-5 1.2829781515235686E-4 13 6.414890757617843E-5 0.0 0.0 6.414890757617843E-5 1.9244672272853528E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAACCG 55 0.0030227115 12.115176 5 TAGGACC 520 0.0 11.715775 4 GTCCTAC 945 0.0 10.983553 1 GACAGTC 165 8.185452E-11 10.930396 7 AGGACCT 810 0.0 10.694261 5 GCGCCAC 80 3.7994626E-4 10.678709 13 GGCGAGG 525 0.0 10.663303 19 TAGGGGT 125 2.1783671E-7 10.661354 4 GTGCTAG 170 1.3278623E-10 10.642724 1 TCGAACT 100 2.430565E-5 10.437386 19 TGGCGAG 1015 0.0 10.286438 18 TTAGGAC 705 0.0 10.26235 3 GCCTCGA 130 3.8835242E-7 10.222355 16 GTCTTAT 150 2.3554094E-8 10.157267 1 TGGGCCG 75 0.0026038974 10.153671 5 ACACCGT 75 0.0026059838 10.152692 6 GTAGGAC 1145 0.0 10.143239 3 TGCGTTG 525 0.0 10.124998 12 GACGTGA 615 0.0 10.0323925 7 CTCTAAT 105 3.9991843E-5 9.975888 1 >>END_MODULE