FastQCFastQC Report
Thu 26 May 2016
SRR1513169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219922
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30110.13563539619860518No Hit
GTCCTAAAGTGTGTATTTCTCATTT28080.1264909307624322No Hit
CTGTAGGACGTGGAATATGGCAAGA23690.10671546117386106No Hit
GTCCTACAGTGTGCATTTCTCATTT22230.10013865352025882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAG400.00523853914.266965
GGTCGAC500.001515607513.2816247
TAGGACC6200.013.2223774
AATCCCG1155.4296834E-912.37557819
GTCGAGG1105.0535164E-711.21302419
GGCGTCC855.4005173E-511.161038
GTAGGAC19000.011.08773
TCGTTCC600.005930818611.06976614
TGTCCGA600.005930818611.06976610
GGGTCGA600.0059417711.0670226
GTCCTAA10100.010.9747041
TGTAGGA19400.010.9653032
GGCGAGG7100.010.95790619
AAAGGCG1408.201823E-910.8700655
GGACCTG11750.010.8180996
AGGACCT12800.010.6259135
ATGTCGA1553.741661E-910.40613217
AAGGCGT1553.754394E-910.4040226
GTCCTAC18200.010.3431071
TGGCGAG16650.010.25700518
Produced by FastQC (version 0.11.5)