##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513169_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2219922
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.590377049283713	31.0	30.0	34.0	25.0	34.0
2	29.457894016096063	31.0	30.0	34.0	23.0	34.0
3	29.67575212102047	31.0	30.0	34.0	25.0	34.0
4	32.75278861149175	37.0	33.0	37.0	25.0	37.0
5	32.93351478114997	37.0	35.0	37.0	26.0	37.0
6	33.29380356607124	37.0	35.0	37.0	27.0	37.0
7	33.25282284692886	37.0	35.0	37.0	27.0	37.0
8	33.14446228290904	37.0	35.0	37.0	27.0	37.0
9	34.39917889006911	39.0	35.0	39.0	26.0	39.0
10	33.976324843845866	39.0	34.0	39.0	22.0	39.0
11	34.45253526925721	39.0	35.0	39.0	27.0	39.0
12	34.44831394976941	39.0	35.0	39.0	27.0	39.0
13	34.494651163419256	39.0	35.0	39.0	27.0	39.0
14	34.72449977972199	40.0	34.0	41.0	17.0	41.0
15	35.17375160028145	40.0	35.0	41.0	25.0	41.0
16	35.034269672538045	40.0	35.0	41.0	23.0	41.0
17	34.655757274354684	39.0	34.0	41.0	17.0	41.0
18	34.38742757628421	39.0	33.0	41.0	17.0	41.0
19	35.019875923568485	40.0	34.0	41.0	24.0	41.0
20	35.22744177498128	40.0	35.0	41.0	25.0	41.0
21	35.273267258939725	40.0	36.0	41.0	25.0	41.0
22	34.96366043491618	40.0	35.0	41.0	21.0	41.0
23	34.512623866964695	40.0	34.0	41.0	18.0	41.0
24	34.7760403293449	40.0	35.0	41.0	19.0	41.0
25	34.42400273523124	39.0	34.0	41.0	17.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.676059250546249
1101	2	0.9393527046761285
1101	3	0.8438075695792158
1101	4	1.1416159556115062
1101	5	0.9226916522130111
1101	6	0.6908303540586402
1101	7	0.6447182725333178
1101	8	0.777363998943855
1101	9	1.0979165115576635
1101	10	1.494761654596367
1101	11	1.0100441427541824
1101	12	0.9951662617552586
1101	13	0.751612722569746
1101	14	1.792710036866687
1101	15	1.5182522592451093
1101	16	1.4801880121290196
1101	17	1.8448527581339036
1101	18	1.8995105797783367
1101	19	1.4922035687767092
1101	20	1.0352521930974916
1101	21	1.0828772860293086
1101	22	1.387519680677265
1101	23	1.7328851099613374
1101	24	1.2765339036030028
1101	25	1.5601385917987187
1102	1	0.9395067724302493
1102	2	1.1245139741592851
1102	3	1.0474709125449024
1102	4	1.3217590246638125
1102	5	1.1986793579151467
1102	6	0.9455438556724545
1102	7	0.9437744342177439
1102	8	0.9518347950170352
1102	9	1.2668614332026351
1102	10	1.4935927521134076
1102	11	1.0605645632648901
1102	12	0.99674971166246
1102	13	1.1090807134161054
1102	14	2.0352811055115083
1102	15	1.571459833605985
1102	16	1.6911437521597819
1102	17	2.0128951371848913
1102	18	2.3019456856141574
1102	19	1.6501385839209561
1102	20	1.4107701413312483
1102	21	1.2215848939336382
1102	22	1.4864736714789046
1102	23	1.8332045927460925
1102	24	1.6055672805842391
1102	25	1.9493097702672983
1103	1	0.6280749354106945
1103	2	0.900618766720747
1103	3	0.6566738230685871
1103	4	1.1954242727684061
1103	5	0.9804962085009237
1103	6	0.7401799520975487
1103	7	0.7891802471838929
1103	8	0.6953283678685835
1103	9	0.9984870072003531
1103	10	1.2472290324592308
1103	11	0.9202288577857587
1103	12	0.8741550058728293
1103	13	0.8336933480207236
1103	14	1.7967875185421676
1103	15	1.277041881384676
1103	16	1.3901830316500678
1103	17	1.6755256086928085
1103	18	1.9030633862174895
1103	19	1.3507482485319073
1103	20	1.1021512497218993
1103	21	1.1186043752187516
1103	22	1.307779734563617
1103	23	1.6995506914504759
1103	24	1.3975538085122992
1103	25	1.5320243954900974
1104	1	0.37652195143490985
1104	2	0.6732462680419431
1104	3	0.5251593157977119
1104	4	0.9725774727458045
1104	5	0.7680652701617134
1104	6	0.45573008451945896
1104	7	0.4078578494677032
1104	8	0.4474643408101855
1104	9	0.7282406818447029
1104	10	0.969658925934084
1104	11	0.5663807243801955
1104	12	0.5929089796166949
1104	13	0.4829220850782434
1104	14	1.5204400733677872
1104	15	1.0568342613570607
1104	16	1.1625616927612512
1104	17	1.5238233016965452
1104	18	1.5657264714997723
1104	19	1.0093426924609545
1104	20	0.7178664820492813
1104	21	0.7941774870987857
1104	22	1.096886239496115
1104	23	1.338211088427137
1104	24	1.2003797792678057
1104	25	1.5006470998824781
1105	1	0.7895281745431006
1105	2	1.073434940116659
1105	3	0.8950010450508508
1105	4	1.372134398525489
1105	5	1.2392062869053788
1105	6	0.8764025381531795
1105	7	0.8035860126595011
1105	8	0.9820820533325758
1105	9	1.18254405448441
1105	10	1.630887619590581
1105	11	0.9563641055867151
1105	12	0.8565886878957443
1105	13	0.8240502010552149
1105	14	1.7097618667147643
1105	15	1.2593599173332493
1105	16	1.5351253883076552
1105	17	1.771963492648858
1105	18	1.3754905228235188
1105	19	1.3094041166599197
1105	20	1.1739675562145564
1105	21	1.0308960719721085
1105	22	1.1199063668263634
1105	23	1.5214515865378857
1105	24	1.3990321162423527
1105	25	1.4572540203921562
1106	1	0.5761913156280727
1106	2	0.8347149894114558
1106	3	0.7224207450243334
1106	4	1.174971754755859
1106	5	1.0596481309795251
1106	6	0.7215141650257877
1106	7	0.7081654253212264
1106	8	0.7038159853325823
1106	9	0.9865853651894483
1106	10	0.780198349486497
1106	11	0.7463479206815222
1106	12	0.7533815476060326
1106	13	0.6841417735931756
1106	14	1.699264154840435
1106	15	1.286775304743088
1106	16	1.4821951566334448
1106	17	1.7114099824139188
1106	18	1.8098422485707886
1106	19	1.2795204261360666
1106	20	1.1851425781382758
1106	21	1.0320447488830666
1106	22	1.306922516829026
1106	23	1.582405562164226
1106	24	1.2062580541929648
1106	25	1.4925717238496787
1107	1	0.5860264421473005
1107	2	0.8382826821789386
1107	3	0.804785259867927
1107	4	1.129087365435275
1107	5	1.1042922333903604
1107	6	0.8453635374107193
1107	7	0.8122698285888106
1107	8	0.8202647301024584
1107	9	1.1561261103578104
1107	10	1.3796965064898785
1107	11	0.9229729953976076
1107	12	0.9335431833900572
1107	13	0.9471498429296972
1107	14	1.7949953761992674
1107	15	1.1273922105012062
1107	16	1.2743860811203973
1107	17	1.7104100322246367
1107	18	1.8565236093996376
1107	19	1.3214311653271409
1107	20	1.1113915819238898
1107	21	0.9042852350356867
1107	22	1.3218321103735562
1107	23	1.631704476290551
1107	24	1.485524342298163
1107	25	1.7221206877867132
1108	1	0.5788429604460141
1108	2	0.7556072900636366
1108	3	0.5827402549223315
1108	4	1.1152405730091033
1108	5	1.0170050707508267
1108	6	0.627601773951632
1108	7	0.5994536635471235
1108	8	0.6872502112385348
1108	9	0.9792043921156193
1108	10	1.1496246186843138
1108	11	0.8442505494459311
1108	12	0.5915806639609187
1108	13	0.6987871892628945
1108	14	1.6165970435724475
1108	15	1.212026412846832
1108	16	1.2287461560108852
1108	17	1.542892899258696
1108	18	1.8228643964562963
1108	19	1.2570041482294982
1108	20	1.111948844888424
1108	21	1.0379682928285305
1108	22	1.2981965656512173
1108	23	1.583581154261438
1108	24	1.3777297259352963
1108	25	1.6473158622956916
1109	1	0.3924818412083333
1109	2	0.6060769477725003
1109	3	0.5752077950791978
1109	4	0.8070845149993247
1109	5	0.8966757008618558
1109	6	0.5833581718842282
1109	7	0.445863054611614
1109	8	0.47835074619904105
1109	9	0.7039882833960434
1109	10	0.9668905038071145
1109	11	0.6246706815143028
1109	12	0.5159417414048093
1109	13	0.3811538670745662
1109	14	1.3469990649987338
1109	15	0.8703038469867863
1109	16	0.8931444647555367
1109	17	1.206666739132757
1109	18	1.44470687566389
1109	19	0.7713221780702639
1109	20	0.7147408441639982
1109	21	0.6551822839961119
1109	22	0.8152216140623523
1109	23	1.0855064548284403
1109	24	0.8958278298903863
1109	25	0.9908874550570062
1110	1	0.28947509314816955
1110	2	0.5793735375811515
1110	3	0.5120560750695518
1110	4	0.655610687993196
1110	5	0.5603199904461889
1110	6	0.21578502193142413
1110	7	0.19945951734507616
1110	8	0.30031907342377195
1110	9	0.42906842044111215
1110	10	0.7722762342034173
1110	11	0.3803668471941961
1110	12	0.28822867595087587
1110	13	0.19074002486844677
1110	14	1.0131674927395338
1110	15	0.7340328193121763
1110	16	0.9006938953688106
1110	17	1.1666227327095342
1110	18	0.8078277973063024
1110	19	0.5300660030742463
1110	20	0.43693216586851946
1110	21	0.3906077100657299
1110	22	0.5711008844628225
1110	23	-0.6003604145047632
1110	24	0.0026608792454041463
1110	25	0.5021605257410471
1111	1	0.11250189068365302
1111	2	0.4613780888968684
1111	3	0.4784514543910632
1111	4	0.7842480628587225
1111	5	0.5882351377475743
1111	6	0.3381195066539462
1111	7	0.3042703681814203
1111	8	0.4388477936394892
1111	9	0.4726005471580237
1111	10	0.7474533188465529
1111	11	0.4544152252640572
1111	12	0.34571521225826274
1111	13	0.09709419744203984
1111	14	0.9291482158053697
1111	15	0.5944441800142215
1111	16	0.7702185713402798
1111	17	1.1069945796413379
1111	18	1.3555784792229204
1111	19	0.8749654969067961
1111	20	0.4700107307165524
1111	21	0.20476749592870647
1111	22	0.4762002811546182
1111	23	0.7742016097048179
1111	24	0.5525063333948879
1111	25	0.7358406729479228
1112	1	0.1529168939802048
1112	2	0.3348738911262856
1112	3	0.28016533943598887
1112	4	0.6833207616923289
1112	5	0.38834792088275094
1112	6	0.20270125510649706
1112	7	0.0481525874894686
1112	8	0.20499989228327564
1112	9	0.3490796685927009
1112	10	0.6726655419301437
1112	11	0.3068079424198942
1112	12	0.31968195049110193
1112	13	0.1309474924242835
1112	14	0.6677950757892503
1112	15	0.46494676130703283
1112	16	0.4353624644273424
1112	17	0.9024900163922212
1112	18	1.1487766337978016
1112	19	0.4412115412456217
1112	20	0.3573307184480683
1112	21	0.33436175839865
1112	22	0.4974321692376833
1112	23	0.9131503517421748
1112	24	0.7157150417663587
1112	25	0.9715475237384865
1113	1	0.07089375469995218
1113	2	0.16412288557284782
1113	3	0.047031117773013165
1113	4	0.34946361408132276
1113	5	0.3782965760709871
1113	6	0.11120780266526253
1113	7	0.14028764059987964
1113	8	0.07856170727580292
1113	9	0.26307740814735325
1113	10	0.5683290635565186
1113	11	0.18207933591190795
1113	12	0.019834905217791743
1113	13	0.008048166033162829
1113	14	0.45361153538409127
1113	15	0.1787729923843031
1113	16	0.3708444896733667
1113	17	0.6250104153433469
1113	18	0.49483472786555893
1113	19	0.37737251406545624
1113	20	0.2178637305980189
1113	21	0.043007303475526726
1113	22	0.3287400315032514
1113	23	0.4686240313914425
1113	24	0.2365257664567082
1113	25	0.6729618941091786
1114	1	-0.9722419472429102
1114	2	-0.7787250622642041
1114	3	-0.8075096539958935
1114	4	-0.5113314497528165
1114	5	-3.3379787049275436
1114	6	-1.3471869528428044
1114	7	-1.118371993357357
1114	8	-0.8014717146805381
1114	9	-0.5096977499492326
1114	10	-0.3203213164719543
1114	11	-0.8091984273142216
1114	12	-0.9480774365560336
1114	13	-0.9166646615431304
1114	14	-6.357099432191685
1114	15	-2.7146497149646365
1114	16	-1.6014833376069006
1114	17	-0.6961041382507673
1114	18	-6.659176955075537
1114	19	-2.642838923348201
1114	20	-1.6262141350963049
1114	21	-1.3926511687300902
1114	22	-1.2115170927203138
1114	23	-0.7737139482572886
1114	24	-0.8606761928789908
1114	25	-0.7334823142836768
1115	1	-0.8176760451534548
1115	2	-0.46736019373127746
1115	3	-0.4934302770036787
1115	4	-3.092667914448615
1115	5	-0.9431683864329727
1115	6	-0.7830727661700934
1115	7	-0.7749858829443568
1115	8	-0.4804573440661457
1115	9	-0.363234923598597
1115	10	-0.670662106477117
1115	11	-0.7873635972005459
1115	12	-0.8073529267618369
1115	13	-0.7946272383705022
1115	14	-6.074060375370934
1115	15	-2.4292841213557637
1115	16	-1.3296002624974506
1115	17	-0.5035048703102092
1115	18	-0.3972985198198913
1115	19	-0.6012460611073109
1115	20	-0.6308160538892338
1115	21	-0.8303361647591245
1115	22	-1.9406139334669987
1115	23	-0.998887102169526
1115	24	-0.8341673099801952
1115	25	-0.4897329330625695
1116	1	-0.5153964490176612
1116	2	-0.27176313167495536
1116	3	-0.27996471931434996
1116	4	-0.05636736791947072
1116	5	-0.7834026353390442
1116	6	-0.5044776531249511
1116	7	-0.3932129640785931
1116	8	-0.376000347874772
1116	9	-0.4400127973661583
1116	10	-0.31733258181294133
1116	11	-0.5182324844211053
1116	12	-0.8333317348260465
1116	13	-0.8008770833041936
1116	14	0.0443550826099397
1116	15	-0.34281265073381917
1116	16	-0.33409780893825314
1116	17	0.013818710834726744
1116	18	-0.059451537216595796
1116	19	-0.31555478568579076
1116	20	-0.8319124727731833
1116	21	-0.8086761748592792
1116	22	-0.8200139604304866
1116	23	-1.5021678129449043
1116	24	-1.0698399380629908
1116	25	-0.5742901142308199
1201	1	0.8480591524276733
1201	2	1.0934979692283378
1201	3	1.0964908673445635
1201	4	1.4362385670374138
1201	5	1.3149981746453676
1201	6	1.054664828020421
1201	7	1.0003873185754557
1201	8	1.1613500661060172
1201	9	1.3176192122714738
1201	10	1.7227288829918805
1201	11	1.2243498311786425
1201	12	1.227688154462598
1201	13	1.1399012893026423
1201	14	2.0355049445125744
1201	15	1.6749750987967076
1201	16	1.7516752444492738
1201	17	2.1019960357849428
1201	18	1.5164841122923818
1201	19	1.4369407336404478
1201	20	1.509781101283039
1201	21	1.387874465120241
1201	22	1.6033825109076005
1201	23	1.946232179895354
1201	24	1.6204208710031054
1201	25	1.9428010393666
1202	1	0.8961557084460914
1202	2	1.1097227496222253
1202	3	1.1520747550651507
1202	4	1.5112774839719592
1202	5	1.3039690676433722
1202	6	1.0134199445229228
1202	7	0.9905857901665769
1202	8	1.0960272990785498
1202	9	1.4295830897160755
1202	10	1.6336983969456327
1202	11	1.2338520573228067
1202	12	1.1810153374877501
1202	13	1.2490581813723693
1202	14	2.0919859750139054
1202	15	1.6847407185739627
1202	16	1.76191035048479
1202	17	2.257442783093225
1202	18	2.372044506599721
1202	19	1.8056024303996878
1202	20	1.5241432169126483
1202	21	1.4629557848006556
1202	22	1.7294922881785837
1202	23	2.0315575827691887
1202	24	1.682650338619439
1202	25	2.004310806323403
1203	1	0.7004799687769498
1203	2	0.8807471000867579
1203	3	0.7978271064646805
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2211	13	-0.4291760307674153
2211	14	0.16989318940180453
2211	15	-0.13671850889384984
2211	16	-6.746898151456758
2211	17	-1.8535933910330797
2211	18	-0.64972128126049
2211	19	-0.6429771731155327
2211	20	-0.7022414141930113
2211	21	-0.6664127007910068
2211	22	-0.5197413108404234
2211	23	-0.11089081289809144
2211	24	-0.5545660930175913
2211	25	-7.796976747324667
2212	1	-1.481425912626385
2212	2	-3.8190913655710013
2212	3	-1.7771576574859296
2212	4	-3.498359197301969
2212	5	-1.2981775629393866
2212	6	-1.103682249128191
2212	7	-1.0151340301509464
2212	8	-0.8087067416638192
2212	9	-0.9521993693667881
2212	10	-0.4176412837664074
2212	11	-0.9138579753084386
2212	12	-1.0127736377309162
2212	13	-1.046116669282661
2212	14	-6.66526470914328
2212	15	-5.117566590776317
2212	16	-2.8300179935936
2212	17	-7.359961083511376
2212	18	-3.0207158144072714
2212	19	-1.8191706828887817
2212	20	-1.9547415301994633
2212	21	-1.4893841018579508
2212	22	-1.4083821949747488
2212	23	-0.8285708254189217
2212	24	-1.185956259288261
2212	25	-0.9576937379835897
2213	1	-0.2743230202182936
2213	2	-0.2125717725061591
2213	3	-0.3292201164761579
2213	4	0.06379358663343027
2213	5	-0.10422351161732024
2213	6	-0.35594214615302633
2213	7	-0.3430681130394575
2213	8	-0.30885393349200996
2213	9	-0.22993379747629206
2213	10	-4.095121081835771
2213	11	-1.6949321505317343
2213	12	-1.072610810465548
2213	13	-0.9107418621394245
2213	14	0.02986480784289114
2213	15	-0.5102902011731416
2213	16	-0.3971288958381294
2213	17	0.03885665280835582
2213	18	0.08111078297874741
2213	19	-0.45203273636256824
2213	20	-0.7330688635446165
2213	21	-0.5751694500641662
2213	22	-0.8866260309474896
2213	23	-1.199295907734033
2213	24	-0.6224441676864743
2213	25	-0.3376431370980768
2214	1	-1.0310982819145096
2214	2	-0.8160396924662834
2214	3	-0.7696794200437722
2214	4	-0.4590035364884102
2214	5	-3.7672431655374226
2214	6	-1.4945229337340287
2214	7	-1.2119433533727175
2214	8	-0.9409923654509953
2214	9	-0.9004002220294467
2214	10	-4.437311140939951
2214	11	-2.0635773830319124
2214	12	-1.452726526480646
2214	13	-1.1706670917912092
2214	14	-0.4890837786414721
2214	15	-0.7666431884265776
2214	16	-0.7738183496585407
2214	17	-0.46153927398790984
2214	18	-0.13166426508790607
2214	19	-0.9288841926041158
2214	20	-1.1948505341278306
2214	21	-1.1837295867870878
2214	22	-0.851558381584816
2214	23	-0.644398093876589
2214	24	-1.1215262726498025
2214	25	-0.8314763636166234
2215	1	-2.223258334755588
2215	2	-4.850358581413111
2215	3	-2.976087443544145
2215	4	-1.915234438089282
2215	5	-1.9387781640599577
2215	6	-1.8080672837366336
2215	7	-2.072919522742275
2215	8	-2.2633709091926306
2215	9	-8.308225220725802
2215	10	-3.872377453015183
2215	11	-3.0868062509466014
2215	12	-3.556644761866785
2215	13	-2.817993576642852
2215	14	-8.29610886077111
2215	15	-4.886839604271948
2215	16	-9.54196421884211
2215	17	-4.63262755682673
2215	18	-3.174557661691477
2215	19	-3.6322058800421892
2215	20	-3.2989735286872843
2215	21	-3.3781200892987755
2215	22	-9.578254597537956
2215	23	-10.713151635445485
2215	24	-6.903466096396997
2215	25	-5.410202615550364
2216	1	-1.693023545570707
2216	2	-1.7257317316190033
2216	3	-1.5666805961870942
2216	4	-6.8765840648907215
2216	5	-3.324181158545631
2216	6	-2.433202627448736
2216	7	-1.9768834156123347
2216	8	-7.8769619516535165
2216	9	-3.648020817388968
2216	10	-1.9233579817018622
2216	11	-1.881337765384231
2216	12	-2.2491670580388643
2216	13	-2.0261319937548663
2216	14	-1.2418479757325116
2216	15	-1.962254726018429
2216	16	-1.6201473346777604
2216	17	-1.4056002056951442
2216	18	-7.441433412392133
2216	19	-4.565541147547901
2216	20	-3.976074861912302
2216	21	-3.225388941193394
2216	22	-2.7345771169135276
2216	23	-2.076152271788196
2216	24	-2.475503572920978
2216	25	-1.8845508478135216
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91255.0
3	3.0
4	754.0
5	4577.0
6	5441.0
7	4961.0
8	4611.0
9	4379.0
10	4263.0
11	4045.0
12	3841.0
13	3951.0
14	3883.0
15	4049.0
16	4050.0
17	4398.0
18	5099.0
19	6219.0
20	7878.0
21	10103.0
22	12814.0
23	14986.0
24	17450.0
25	20118.0
26	22869.0
27	25907.0
28	28930.0
29	33974.0
30	41368.0
31	49654.0
32	63238.0
33	83150.0
34	109724.0
35	147746.0
36	212349.0
37	310532.0
38	847353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.413689107587444	18.741417287585342	12.182160413405255	25.66273319142196
2	14.168380513237008	20.477579363555755	41.8560856723029	23.497954450904338
3	18.253012129827503	25.14347508513646	29.969161608619395	26.634351176416647
4	11.114797986633995	17.018411190034392	39.9031795302659	31.963611293065714
5	12.341676238777142	38.23271814361734	36.85912217235038	12.56648344525513
6	31.187981862679727	37.95275718265997	18.55524998287912	12.304010971781185
7	27.482723726502783	31.680977581619075	23.070909990367277	17.76538870151087
8	24.493299104234453	37.09839904773662	21.280040945162856	17.12826090286607
9	26.460839064742203	14.883160358550029	20.376662258757058	38.2793383179507
10	13.415515485748786	29.02886287655556	36.47022248655078	21.085399151144873
11	34.152721833238715	22.36652158633181	25.181260306561054	18.299496273868417
12	22.853908859589083	24.881864785815573	32.38772592359951	19.876500430995833
13	28.821622615358134	21.95172725167237	25.962440510682168	23.26420962228733
14	20.02276348326352	21.894577857240698	29.122704727162617	28.95995393233316
15	22.747687457243984	30.90371007297928	23.637328040825857	22.71127442895088
16	21.797944271954808	28.056411996267556	28.274618521557297	21.87102521022034
17	21.20360923236664	28.44316477510347	28.645870888193475	21.707355104336415
18	21.12035363935815	27.585311890267256	30.234948472654683	21.05938599771991
19	23.091546385992483	27.211721772810897	27.80253257412283	21.894199267073787
20	23.39371698778058	27.482117864832055	28.07646172993267	21.047703417454695
21	22.972339660426094	27.00616546815109	27.203089132968085	22.818405738454732
22	22.845984184970764	28.15725976181012	27.3916108057048	21.60514524751431
23	21.928365246402176	27.756844855352554	28.124895798505626	22.189894099739643
24	22.748815806671143	27.278270784790042	28.194798112501747	21.778115296037065
25	22.500435992081393	27.32171979611343	28.09231045159767	22.08553376020751
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	182.0
2	309.5
3	437.0
4	437.0
5	437.0
6	860.0
7	1283.0
8	1283.0
9	1283.0
10	2269.0
11	3255.0
12	3255.0
13	3255.0
14	6137.5
15	9020.0
16	9020.0
17	9020.0
18	17403.5
19	25787.0
20	25787.0
21	25787.0
22	42130.5
23	58474.0
24	58474.0
25	58474.0
26	83696.0
27	108918.0
28	108918.0
29	108918.0
30	140462.0
31	172006.0
32	172006.0
33	172006.0
34	198323.0
35	224640.0
36	224640.0
37	224640.0
38	244809.0
39	264978.0
40	264978.0
41	264978.0
42	276268.0
43	287558.0
44	287558.0
45	287558.0
46	290910.0
47	294262.0
48	294262.0
49	294262.0
50	280418.5
51	266575.0
52	266575.0
53	266575.0
54	234419.5
55	202264.0
56	202264.0
57	202264.0
58	170688.5
59	139113.0
60	139113.0
61	139113.0
62	111912.0
63	84711.0
64	84711.0
65	84711.0
66	64467.5
67	44224.0
68	44224.0
69	44224.0
70	32154.5
71	20085.0
72	20085.0
73	20085.0
74	13946.5
75	7808.0
76	7808.0
77	7808.0
78	5377.5
79	2947.0
80	2947.0
81	2947.0
82	1956.5
83	966.0
84	966.0
85	966.0
86	642.5
87	319.0
88	319.0
89	319.0
90	199.5
91	80.0
92	80.0
93	80.0
94	51.5
95	23.0
96	23.0
97	23.0
98	15.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17951081164112975
2	0.27437900971295387
3	0.27582050180141465
4	0.4903325432154823
5	0.2714059322805035
6	0.018469117383403563
7	0.01819883761681717
8	0.016577159017298806
9	0.019234910055398343
10	0.020811542027152304
11	0.020811542027152304
12	0.028964981652508513
13	0.02774872270286974
14	0.026036950847822583
15	0.042163643587477394
16	0.020946681910445502
17	0.04261410986512139
18	0.03207319896825204
19	0.026982930030874958
20	0.030091147346618483
21	0.035496742678346356
22	0.04594756031968691
23	0.029910960835560888
24	0.05009185007401161
25	0.038064400460917094
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2219922.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.47826082155596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.2003840716569	65.15977195128919
2	10.59358683736116	16.20358194367018
3	2.393328461135047	5.491127948470178
4	0.7856174417348738	2.403306224598529
5	0.3288808547420952	1.2576117894091108
6	0.19348205227033688	0.8878302514692448
7	0.11217125667594541	0.6005063837321256
8	0.07302812766859222	0.4468051356118787
9	0.050729586643116366	0.3491739502795777
>10	0.24237703003276728	3.2100791728056812
>50	0.014625135796783253	0.7706746003065982
>100	0.00982642004444165	1.5512645937451859
>500	0.0011300533490606487	0.5422456003123814
>1k	8.326708887799333E-4	1.1260204543000785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3011	0.13563539619860518	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2808	0.1264909307624322	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2369	0.10671546117386106	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2223	0.10013865352025882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.504662776439893E-5	0.0
8	0.0	0.0	0.0	4.504662776439893E-5	0.0
9	0.0	0.0	0.0	4.504662776439893E-5	0.0
10	0.0	0.0	0.0	4.504662776439893E-5	0.0
11	0.0	0.0	0.0	1.8018651105759572E-4	0.0
12	0.0	0.0	0.0	2.2523313882199465E-4	0.0
13	0.0	0.0	0.0	3.153263943507925E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACAG	40	0.005238539	14.26696	5
GGTCGAC	50	0.0015156075	13.281624	7
TAGGACC	620	0.0	13.222377	4
AATCCCG	115	5.4296834E-9	12.375578	19
GTCGAGG	110	5.0535164E-7	11.213024	19
GGCGTCC	85	5.4005173E-5	11.16103	8
GTAGGAC	1900	0.0	11.0877	3
TCGTTCC	60	0.0059308186	11.069766	14
TGTCCGA	60	0.0059308186	11.069766	10
GGGTCGA	60	0.00594177	11.067022	6
GTCCTAA	1010	0.0	10.974704	1
TGTAGGA	1940	0.0	10.965303	2
GGCGAGG	710	0.0	10.957906	19
AAAGGCG	140	8.201823E-9	10.870065	5
GGACCTG	1175	0.0	10.818099	6
AGGACCT	1280	0.0	10.625913	5
ATGTCGA	155	3.741661E-9	10.406132	17
AAGGCGT	155	3.754394E-9	10.404022	6
GTCCTAC	1820	0.0	10.343107	1
TGGCGAG	1665	0.0	10.257005	18
>>END_MODULE