FastQCFastQC Report
Thu 26 May 2016
SRR1513169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219922
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30500.1373922146814167No Hit
GTATCAACGCAGAGTACTTTTTTTT29530.13302269178827003No Hit
GTCCTAAAGTGTGTATTTCTCATTT28280.12739186331772018No Hit
CTTTAGGACGTGAAATATGGCGAGG27340.12315748030786668No Hit
CTGTAGGACGTGGAATATGGCAAGA26810.12077000903635354No Hit
GTCCTACAGTGTGCATTTCTCATTT22950.10338201071929554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGA655.506503E-513.14055917
CCCCTAT1750.012.5187891
TAGGACC7350.012.04680254
ACCGTGC1001.9552863E-611.3879718
CTACACT3500.011.1530074
TAGTACG600.005781821411.1076714
AGGACCT12150.011.0488995
GGCGAGG7600.010.86205719
CTATACT3250.010.8391334
TGTAGGA19300.010.8046852
GTAGGAC17550.010.7414843
GGACCTG12350.010.6372526
CTGTAGG19700.010.3954851
TGGCGAG17900.010.33824218
GTCCTAC18650.010.2657281
AGGACTG3950.010.1234465
CGAAATC1606.2791514E-910.08355313
GTCCTAA10300.010.08002951
GGACGTG16650.010.0627296
TCTACAC3600.010.0497973
Produced by FastQC (version 0.11.5)