##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513169_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2219922 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.932999447728342 31.0 31.0 34.0 28.0 34.0 2 31.024595008293083 33.0 31.0 34.0 27.0 34.0 3 31.13177039553642 33.0 31.0 34.0 27.0 34.0 4 34.527751875966814 37.0 35.0 37.0 31.0 37.0 5 34.29584462877524 37.0 35.0 37.0 30.0 37.0 6 34.21804639982846 37.0 35.0 37.0 30.0 37.0 7 34.08910898671215 37.0 35.0 37.0 30.0 37.0 8 34.05655559069193 37.0 35.0 37.0 30.0 37.0 9 35.63135416469588 39.0 37.0 39.0 30.0 39.0 10 35.4726508408854 39.0 35.0 39.0 30.0 39.0 11 35.497790462908156 39.0 37.0 39.0 30.0 39.0 12 35.438142421220206 39.0 35.0 39.0 30.0 39.0 13 35.413573539971225 39.0 35.0 39.0 30.0 39.0 14 36.58803147137602 40.0 37.0 41.0 30.0 41.0 15 36.57556797040617 40.0 37.0 41.0 30.0 41.0 16 36.54391145274474 40.0 37.0 41.0 29.0 41.0 17 36.4782686058339 40.0 37.0 41.0 29.0 41.0 18 36.43148858383312 40.0 37.0 41.0 29.0 41.0 19 36.51885291465196 40.0 37.0 41.0 29.0 41.0 20 36.489850093832125 40.0 37.0 41.0 29.0 41.0 21 36.4364117297815 40.0 37.0 41.0 29.0 41.0 22 36.38336842465636 40.0 37.0 41.0 29.0 41.0 23 36.30549361644238 40.0 37.0 41.0 29.0 41.0 24 36.249993017772695 40.0 37.0 41.0 29.0 41.0 25 36.194738824156886 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28913014652887625 1101 2 0.3190569682566782 1101 3 0.3022472539719061 1101 4 0.44781703221422475 1101 5 0.3143813839391001 1101 6 0.2837121455499343 1101 7 0.4066255350739638 1101 8 0.31882930865287307 1101 9 0.413415668450007 1101 10 0.3485130564804564 1101 11 0.3047308859394704 1101 12 0.35408117338728573 1101 13 0.3850123856542069 1101 14 0.49993918356538813 1101 15 0.47984917369673497 1101 16 0.44990247460401633 1101 17 0.4182022624665862 1101 18 0.43077016061073437 1101 19 0.33646249738063005 1101 20 0.40723990684378464 1101 21 0.43709066389632767 1101 22 0.3854705458526553 1101 23 0.4089736140106126 1101 24 0.49395332699607053 1101 25 0.4352471659600994 1102 1 0.40570056418217604 1102 2 0.4617332391188427 1102 3 0.4511194732815049 1102 4 0.5393392839491682 1102 5 0.5149911294312304 1102 6 0.5128640427429687 1102 7 0.45273471778143914 1102 8 0.4641093468178781 1102 9 0.40566270730823817 1102 10 0.49338361601015635 1102 11 0.6171252178407869 1102 12 0.6510018841110039 1102 13 0.5948518988516938 1102 14 0.7049473458063886 1102 15 0.6428208754432347 1102 16 0.6257010946013821 1102 17 0.729509687000025 1102 18 0.7218276739722072 1102 19 0.7385757848132641 1102 20 0.6579586064509542 1102 21 0.7448631547836868 1102 22 0.6684500992525955 1102 23 0.7066931062372319 1102 24 0.7554475761568611 1102 25 0.8136067170244203 1103 1 0.3914046948237271 1103 2 0.3657857167959371 1103 3 0.3733559571766598 1103 4 0.4831844579382718 1103 5 0.36959424475050184 1103 6 0.27877355121115954 1103 7 0.4012815054534755 1103 8 0.4180835973311545 1103 9 0.42151825173940694 1103 10 0.47562109886069237 1103 11 0.36435630533223673 1103 12 0.39439001167234977 1103 13 0.486587334573926 1103 14 0.5081528436829288 1103 15 0.560370447630369 1103 16 0.5103270057708826 1103 17 0.46514286357395207 1103 18 0.3508646196456837 1103 19 0.30398268046789667 1103 20 0.38878783970207564 1103 21 0.27336303049845156 1103 22 0.3760955838136226 1103 23 0.3169638085408337 1103 24 0.34695300873131885 1103 25 0.26732504607389984 1104 1 0.09488333736165089 1104 2 0.15785874846893222 1104 3 0.12003548971983946 1104 4 0.2780326153398178 1104 5 0.099866289262863 1104 6 0.10359401120466316 1104 7 0.0831962637941075 1104 8 0.11512895521420319 1104 9 0.13646073790818036 1104 10 0.17084276220539607 1104 11 0.20996843240966 1104 12 0.14466610715972905 1104 13 0.06732974754551435 1104 14 0.20349864371554105 1104 15 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-0.9758965060559319 2215 19 -0.9067396023569003 2215 20 -0.9804241351509617 2215 21 -0.9881586799356938 2215 22 -0.8966816851184731 2215 23 -0.8501864909762631 2215 24 -1.000482368541988 2215 25 -1.1111045400766457 2216 1 -0.3521881853634987 2216 2 -0.23332277941119628 2216 3 -0.25622946923062173 2216 4 -0.12921064772666568 2216 5 -0.2698373167999506 2216 6 -0.29752901286458666 2216 7 -0.5002572946975974 2216 8 -0.4220872182698372 2216 9 -0.4589357629342601 2216 10 -0.3940130886946491 2216 11 -0.392475511529085 2216 12 -0.5535497168929169 2216 13 -0.48968465525547344 2216 14 -0.7425522625572754 2216 15 -0.6894688177813038 2216 16 -0.588369071592723 2216 17 -0.7380972789411402 2216 18 -0.6339841511430393 2216 19 -0.6276789525136692 2216 20 -0.7110231289944906 2216 21 -0.9638180876534292 2216 22 -0.8557861246243306 2216 23 -0.8206944239842642 2216 24 -0.7696611382923066 2216 25 -0.9134464359910908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46330.0 3 0.0 4 848.0 5 6636.0 6 7299.0 7 5675.0 8 4748.0 9 4100.0 10 3724.0 11 3350.0 12 3160.0 13 3004.0 14 2887.0 15 2914.0 16 3027.0 17 3176.0 18 3572.0 19 4152.0 20 5407.0 21 7244.0 22 8920.0 23 11013.0 24 12732.0 25 14491.0 26 16021.0 27 17874.0 28 20182.0 29 24628.0 30 31057.0 31 39776.0 32 50698.0 33 66733.0 34 88812.0 35 119798.0 36 172602.0 37 281351.0 38 1121981.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.019415442633274 19.4179699805304 13.05871298451729 27.50390159231903 2 13.644325350022571 20.75015069508955 40.338710302318226 25.266813652569653 3 16.844882686466846 25.146793958675147 30.212435657980212 27.7958876968778 4 10.997911136117615 17.550105479292228 38.46771000244609 32.98427338214406 5 12.328288482112788 38.497779793574225 36.53545905681465 12.638472667498332 6 31.25730871232411 38.306534909822865 18.153270619100763 12.282885758752261 7 27.285217266701846 31.73198283549572 23.013512338282947 17.969287559519493 8 24.642798411386117 37.36279282465551 20.795117918312087 17.199290845646292 9 26.22251153822763 14.696447194448137 20.036399757635277 39.04464150968896 10 13.858826391329027 29.300664017803246 35.67379337063365 21.166716220234072 11 34.11259494702967 22.289521884102236 25.105611818793633 18.492271350074464 12 22.647957901223556 25.057141647319142 32.37307436927964 19.921826082177663 13 28.817589086463396 21.81905490373085 26.011274269996875 23.352081739808874 14 20.512108993328017 22.030074612174808 28.076124869869034 29.381691524628145 15 22.80080110922816 30.92027557725001 23.461319812137543 22.81760350138428 16 22.0339723647948 28.23455058330878 27.69574786861881 22.03572918327761 17 21.515025220382665 28.747766886617537 27.882006006033066 21.85520188696673 18 21.419688110567183 27.828570759103982 29.4721905798721 21.27955055045673 19 23.106937991515018 27.24519149771929 27.715928757857256 21.931941752908436 20 23.31370201295361 27.514840611516984 27.972829675997623 21.198627699531787 21 22.818474500912878 26.93383449637214 27.295652107321803 22.952038895393176 22 22.854458072081073 28.20015342915974 27.15290706814714 21.792481430612046 23 21.97693931196349 27.83306566597366 27.728461411977236 22.461533610085617 24 22.676021950320777 27.221677158026274 28.22788368239965 21.8744172092533 25 22.470674618905186 27.41080759667015 27.896861148149483 22.22165663627518 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 141.0 1 141.0 2 261.5 3 382.0 4 382.0 5 382.0 6 870.5 7 1359.0 8 1359.0 9 1359.0 10 2202.0 11 3045.0 12 3045.0 13 3045.0 14 5761.0 15 8477.0 16 8477.0 17 8477.0 18 16619.0 19 24761.0 20 24761.0 21 24761.0 22 40443.5 23 56126.0 24 56126.0 25 56126.0 26 81498.5 27 106871.0 28 106871.0 29 106871.0 30 137945.5 31 169020.0 32 169020.0 33 169020.0 34 194564.5 35 220109.0 36 220109.0 37 220109.0 38 240228.0 39 260347.0 40 260347.0 41 260347.0 42 272999.5 43 285652.0 44 285652.0 45 285652.0 46 291193.0 47 296734.0 48 296734.0 49 296734.0 50 284251.5 51 271769.0 52 271769.0 53 271769.0 54 239128.0 55 206487.0 56 206487.0 57 206487.0 58 174426.5 59 142366.0 60 142366.0 61 142366.0 62 114698.5 63 87031.0 64 87031.0 65 87031.0 66 66482.5 67 45934.0 68 45934.0 69 45934.0 70 33251.0 71 20568.0 72 20568.0 73 20568.0 74 14396.0 75 8224.0 76 8224.0 77 8224.0 78 5665.0 79 3106.0 80 3106.0 81 3106.0 82 2060.5 83 1015.0 84 1015.0 85 1015.0 86 660.5 87 306.0 88 306.0 89 306.0 90 191.0 91 76.0 92 76.0 93 76.0 94 45.0 95 14.0 96 14.0 97 14.0 98 8.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18739397149989953 2 0.009369698574994978 3 0.006486714398073445 4 0.002477564527041941 5 0.08491289333589197 6 0.3452824018141178 7 0.03112721978519966 8 0.016982578667178398 9 0.005720921726078664 10 0.004594756031968691 11 0.0 12 0.0 13 0.0 14 0.001937004993869154 15 0.0 16 0.0 17 0.02193770772126228 18 0.03036142711320488 19 0.0 20 0.0 21 8.558859275235797E-4 22 2.2523313882199465E-4 23 0.005720921726078664 24 0.0 25 9.009325552879786E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2219922.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.04028773096088 #Duplication Level Percentage of deduplicated Percentage of total 1 83.78966316048468 62.03810769282578 2 11.359354239559053 16.820997126697254 3 2.7418240829520957 6.090163320283534 4 0.9224393566966216 2.7319070153672134 5 0.3902179680366516 1.4445925315612294 6 0.21339585437629505 0.9479934275169069 7 0.12525149258468535 0.6491559584791693 8 0.08654578957955203 0.5126300129898582 9 0.05828331383907579 0.3883781993903159 >10 0.2812866677158085 3.5723925427783367 >50 0.017670142774678734 0.8997885742361625 >100 0.01145186052907275 1.7093783711745005 >500 0.0017034880095008525 0.8369410261528237 >1k 9.125828622310912E-4 1.3575742005468865 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3050 0.1373922146814167 No Hit GTATCAACGCAGAGTACTTTTTTTT 2953 0.13302269178827003 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2828 0.12739186331772018 No Hit CTTTAGGACGTGAAATATGGCGAGG 2734 0.12315748030786668 No Hit CTGTAGGACGTGGAATATGGCAAGA 2681 0.12077000903635354 No Hit GTCCTACAGTGTGCATTTCTCATTT 2295 0.10338201071929554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.504662776439893E-5 0.0 6 0.0 0.0 0.0 4.504662776439893E-5 0.0 7 0.0 0.0 0.0 9.009325552879786E-5 0.0 8 0.0 0.0 0.0 9.009325552879786E-5 0.0 9 0.0 0.0 0.0 1.351398832931968E-4 0.0 10 0.0 0.0 0.0 1.8018651105759572E-4 0.0 11 0.0 0.0 0.0 3.153263943507925E-4 0.0 12 0.0 0.0 0.0 3.6037302211519145E-4 0.0 13 0.0 0.0 0.0 3.6037302211519145E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGCGA 65 5.506503E-5 13.140559 17 CCCCTAT 175 0.0 12.518789 1 TAGGACC 735 0.0 12.0468025 4 ACCGTGC 100 1.9552863E-6 11.387971 8 CTACACT 350 0.0 11.153007 4 TAGTACG 60 0.0057818214 11.107671 4 AGGACCT 1215 0.0 11.048899 5 GGCGAGG 760 0.0 10.862057 19 CTATACT 325 0.0 10.839133 4 TGTAGGA 1930 0.0 10.804685 2 GTAGGAC 1755 0.0 10.741484 3 GGACCTG 1235 0.0 10.637252 6 CTGTAGG 1970 0.0 10.395485 1 TGGCGAG 1790 0.0 10.338242 18 GTCCTAC 1865 0.0 10.265728 1 AGGACTG 395 0.0 10.123446 5 CGAAATC 160 6.2791514E-9 10.083553 13 GTCCTAA 1030 0.0 10.0800295 1 GGACGTG 1665 0.0 10.062729 6 TCTACAC 360 0.0 10.049797 3 >>END_MODULE