FastQCFastQC Report
Thu 26 May 2016
SRR1513168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977597
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24780.1253035881425791No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACG400.00517121914.2946884
TAGGACC5800.013.4731544
GACCGAC550.003099693512.0748287
GTAGGAC11500.011.6017813
GGCGAGG4400.010.99977919
AGGACGT9350.010.9853445
GTCCTAC10250.010.9033981
TGTAGGA12600.010.89952
GGACGTG9150.010.887146
CGAAATC803.8138582E-410.67463513
AGGACCT9550.010.6556995
TCCTACA11000.010.4040672
TTAGGAC7700.010.39643
TAGGACG9900.010.2998764
TGTACTG3700.010.2815785
TGGCGAG9600.010.28057918
GACGTGA5550.010.0856677
ACGAAAT1054.160431E-59.94040112
TATGGCG10050.09.91493116
AGGCGTG1356.5619497E-79.8387487
Produced by FastQC (version 0.11.5)