##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513168_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1977597
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.653477933067254	31.0	30.0	34.0	25.0	34.0
2	29.52155924589287	31.0	30.0	34.0	23.0	34.0
3	29.734012541483427	31.0	30.0	34.0	25.0	34.0
4	32.80581281221604	37.0	35.0	37.0	25.0	37.0
5	32.9804363578626	37.0	35.0	37.0	26.0	37.0
6	33.34186793365888	37.0	35.0	37.0	28.0	37.0
7	33.299520579774345	37.0	35.0	37.0	27.0	37.0
8	33.18466907059426	37.0	35.0	37.0	27.0	37.0
9	34.452887519550245	39.0	35.0	39.0	26.0	39.0
10	34.031715764131924	39.0	34.0	39.0	23.0	39.0
11	34.505258654821986	39.0	35.0	39.0	27.0	39.0
12	34.50267268811593	39.0	35.0	39.0	27.0	39.0
13	34.54596512838561	39.0	35.0	39.0	27.0	39.0
14	34.79049371535252	40.0	34.0	41.0	17.0	41.0
15	35.22953968882437	40.0	35.0	41.0	25.0	41.0
16	35.09061148454412	40.0	35.0	41.0	23.0	41.0
17	34.703887596916864	40.0	34.0	41.0	17.0	41.0
18	34.43818381601509	39.0	34.0	41.0	17.0	41.0
19	35.078046234900235	40.0	35.0	41.0	24.0	41.0
20	35.28466012033797	40.0	36.0	41.0	25.0	41.0
21	35.34126922724903	40.0	36.0	41.0	25.0	41.0
22	35.02412827284831	40.0	36.0	41.0	22.0	41.0
23	34.57994373980139	40.0	34.0	41.0	18.0	41.0
24	34.8363427938048	40.0	35.0	41.0	19.0	41.0
25	34.48901874345481	40.0	34.0	41.0	17.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9255582263255668
1101	2	1.1037139071269344
1101	3	1.1013265421004732
1101	4	1.4132594719618226
1101	5	1.2918506710356183
1101	6	1.055211535607718
1101	7	0.9444544273582949
1101	8	1.0559893575342088
1101	9	1.4438515674279984
1101	10	1.7692584780210154
1101	11	1.3343442731715243
1101	12	1.205157669392399
1101	13	1.086635942607522
1101	14	2.0841562558738076
1101	15	1.729701643016334
1101	16	1.806283533771058
1101	17	2.126785292250709
1101	18	2.307021065095995
1101	19	1.77567536621752
1101	20	1.432196759595179
1101	21	1.4514712459384569
1101	22	1.6832558642696611
1101	23	1.7908652147458923
1101	24	1.4404870835504724
1101	25	1.7143307636086504
1102	1	0.9054557035831294
1102	2	1.1802272888145495
1102	3	1.0344418870426395
1102	4	1.3936753573407472
1102	5	1.4075122671618416
1102	6	1.0697850306458392
1102	7	1.0972676994957524
1102	8	1.085633258350235
1102	9	1.4099163444748228
1102	10	1.6981252484928575
1102	11	1.263946649447611
1102	12	1.1315734912675666
1102	13	1.192345610091209
1102	14	2.2304669407272613
1102	15	1.7614389440250164
1102	16	1.838176134829446
1102	17	2.253856314781082
1102	18	2.432508200086822
1102	19	1.914448006574112
1102	20	1.6255198705529281
1102	21	1.5640395665321236
1102	22	1.6093948146958326
1102	23	2.090576272640689
1102	24	1.7395703931934463
1102	25	2.146605301479809
1103	1	0.6432101608449727
1103	2	0.9468734177563221
1103	3	0.722371339048884
1103	4	1.1046430476827496
1103	5	0.8594368458507091
1103	6	0.6649109862710247
1103	7	0.6181213660854752
1103	8	0.6268918513282671
1103	9	0.9178170678995272
1103	10	1.2180111580989958
1103	11	0.8710043568195616
1103	12	0.8138903889684954
1103	13	0.8562579194584146
1103	14	1.6515639702694713
1103	15	1.2773230870233192
1103	16	1.2943220338577532
1103	17	1.6696212905083954
1103	18	1.8496845234220984
1103	19	1.1630173631980725
1103	20	0.950400991052426
1103	21	0.9149321093993237
1103	22	1.185450250801395
1103	23	1.5611392910440642
1103	24	1.2536128051099311
1103	25	1.4827838670015083
1104	1	1.1000821062436401
1104	2	1.344762305851681
1104	3	1.212142159140157
1104	4	1.5776117452352594
1104	5	1.5207766924927597
1104	6	1.18028320424785
1104	7	1.186668822062316
1104	8	1.2074680982245667
1104	9	1.4989012148013003
1104	10	1.6668574025908995
1104	11	1.1584705226089795
1104	12	1.1494982620732372
1104	13	1.2347682857606088
1104	14	2.2064871603623857
1104	15	1.7584393719615292
1104	16	1.8886186036579957
1104	17	2.2683232709256202
1104	18	2.326044021878282
1104	19	1.6712544609667361
1104	20	1.4959964136438018
1104	21	1.393523296911674
1104	22	1.7139909696935618
1104	23	1.9012731519173158
1104	24	1.6574686553038305
1104	25	1.9849931784619343
1105	1	0.6810764959835325
1105	2	0.9027680598437762
1105	3	0.8249917989854936
1105	4	1.074115876940887
1105	5	1.088821065621005
1105	6	0.7725589057659903
1105	7	0.740014243020326
1105	8	0.9262288928504034
1105	9	1.0964604806842715
1105	10	1.4560428663386276
1105	11	1.0116002277699039
1105	12	0.93260775050517
1105	13	0.9090541616185845
1105	14	1.680940566457096
1105	15	1.3703214127672823
1105	16	1.5293588469656214
1105	17	1.8990700175166992
1105	18	1.6119712463664442
1105	19	1.3555831972326757
1105	20	1.1661038120712277
1105	21	1.0398286779074724
1105	22	1.2562616052481417
1105	23	1.4878326793453809
1105	24	1.4364057925867257
1105	25	1.6047946162530806
1106	1	0.48592383448529475
1106	2	0.6528216952053114
1106	3	0.5803439475884673
1106	4	1.0081082837224002
1106	5	0.8595718012567488
1106	6	0.510146703778183
1106	7	0.5733657584755107
1106	8	0.5410653904043627
1106	9	0.802250025091162
1106	10	0.5618637265086193
1106	11	0.5401827510688264
1106	12	0.49683250886135255
1106	13	0.3937496357506234
1106	14	1.435400500500407
1106	15	1.0067867399543715
1106	16	1.0833647593816949
1106	17	1.3232151541217476
1106	18	1.441263214044021
1106	19	0.9863542938082119
1106	20	0.7138216331318219
1106	21	0.6938950582152117
1106	22	0.826931147650825
1106	23	1.1982217691612718
1106	24	0.7940347679805058
1106	25	1.0972205288513521
1107	1	0.799925967514195
1107	2	0.9124015485971277
1107	3	0.9481712623754639
1107	4	1.2211745878742946
1107	5	1.1542414822036946
1107	6	0.7927204585098977
1107	7	0.8693047418318756
1107	8	0.9174961721143902
1107	9	1.1315814256800039
1107	10	1.422631016059782
1107	11	0.9890426320998955
1107	12	0.9633912723054436
1107	13	0.8894347586251072
1107	14	1.729672116194692
1107	15	1.242700487475851
1107	16	1.2847031599104497
1107	17	1.8689964135100823
1107	18	2.177473632838712
1107	19	1.5564175336087303
1107	20	1.4553758901768248
1107	21	1.1683925947170408
1107	22	1.4250579877637861
1107	23	1.6203511929430405
1107	24	1.3887217234572873
1107	25	1.7043226138119962
1108	1	0.6551585427463138
1108	2	0.8819816076351614
1108	3	0.7058401637475384
1108	4	1.1174091981873318
1108	5	1.069338537815085
1108	6	0.7513806587949645
1108	7	0.583725179314385
1108	8	0.6261455382340486
1108	9	0.8858719185093094
1108	10	1.1123658893327146
1108	11	0.7904371361836624
1108	12	0.6343913147710225
1108	13	0.5763502445129305
1108	14	1.5572052692852552
1108	15	1.1472133801326834
1108	16	1.2264285252504834
1108	17	1.5587609037979888
1108	18	1.7863182317078241
1108	19	1.178761393685754
1108	20	1.0243319230647785
1108	21	0.9841719305337051
1108	22	1.1948744384911976
1108	23	1.526321743612634
1108	24	1.0834278998026576
1108	25	1.5131320424758243
1109	1	0.23918019952201064
1109	2	0.4554671567525226
1109	3	0.3524712570749742
1109	4	0.6670043469832905
1109	5	0.6997685725705409
1109	6	0.38528192525257765
1109	7	0.32279500569887176
1109	8	0.2922833682963031
1109	9	0.559582070392544
1109	10	0.887770673128955
1109	11	0.48614164199017296
1109	12	0.37925120093274245
1109	13	0.28826605487593326
1109	14	1.2846577670136128
1109	15	0.7522004782811251
1109	16	0.8439669544332062
1109	17	1.1204101837479428
1109	18	1.2636150174346241
1109	19	0.8153589718585721
1109	20	0.6444890101853034
1109	21	0.475278146341104
1109	22	0.751786298757537
1109	23	1.0819986931134125
1109	24	0.8167837024002793
1109	25	1.04465219605774
1110	1	0.3933710821397298
1110	2	0.5786224175750583
1110	3	0.4657791217470546
1110	4	0.8405983365357699
1110	5	0.7174448338322605
1110	6	0.38949914280044595
1110	7	0.3942805465681758
1110	8	0.40602219729642286
1110	9	0.463796673124655
1110	10	0.9197928809192959
1110	11	0.4847324163865352
1110	12	0.469890213877143
1110	13	0.2702023739731061
1110	14	1.0851439092905437
1110	15	0.7598566588250861
1110	16	0.8981363315253859
1110	17	1.260469961638023
1110	18	0.9909568651061349
1110	19	0.6222204618659717
1110	20	0.45508765891255365
1110	21	0.36491114894471366
1110	22	0.6337349448432974
1110	23	-0.5387095804304707
1110	24	0.15113026794650608
1110	25	0.6504227208939568
1111	1	0.2778073866716362
1111	2	0.6317717249520456
1111	3	0.5806682637118783
1111	4	0.9153267040258655
1111	5	0.7906057219105307
1111	6	0.547873703756018
1111	7	0.4627425302814032
1111	8	0.5507743113564629
1111	9	0.6602235351547634
1111	10	1.0830047908110032
1111	11	0.6716051478885703
1111	12	0.5254941060820428
1111	13	0.4132021040477696
1111	14	1.2945255245790648
1111	15	1.0414758865725346
1111	16	0.9730518902110745
1111	17	1.3236880973779321
1111	18	1.5906289011424661
1111	19	1.0408616699757047
1111	20	0.7008245899061478
1111	21	0.5429479571155227
1111	22	0.8758643138114834
1111	23	1.2026445716957923
1111	24	0.7860259496503801
1111	25	0.924305059014884
1112	1	0.18813725654881353
1112	2	0.4070845398562497
1112	3	0.3518463404771843
1112	4	0.7236111996516144
1112	5	0.4773555442620534
1112	6	0.3242883141914277
1112	7	0.020179670663303284
1112	8	0.2305198988066124
1112	9	0.4544917533823991
1112	10	0.6759912484340944
1112	11	0.097070298224331
1112	12	0.1156222646781373
1112	13	0.11759082531877141
1112	14	0.4629897017391542
1112	15	0.37125494908784873
1112	16	0.2853301249671816
1112	17	0.8133696923381706
1112	18	0.8827029282415992
1112	19	0.33471983534899863
1112	20	0.19312717408666913
1112	21	0.13013398776588048
1112	22	0.38302896397830466
1112	23	0.7694466145325762
1112	24	0.5263433756587759
1112	25	0.7029160693064256
1113	1	0.27277342618239686
1113	2	0.3739743467062766
1113	3	0.26120306862245
1113	4	0.6880872728876497
1113	5	0.7969343670804889
1113	6	0.4592071305644865
1113	7	0.3698302111045422
1113	8	0.41491804905360397
1113	9	0.5912147415257394
1113	10	0.8376553984230313
1113	11	0.4647109646457963
1113	12	0.4149534390226677
1113	13	0.2267098828087697
1113	14	0.9116977855159121
1113	15	0.6618888667376126
1113	16	0.7398920743178579
1113	17	1.1071492691137337
1113	18	0.9281571846407672
1113	19	0.8235290471074848
1113	20	0.5756694377591387
1113	21	0.5159869425356334
1113	22	0.6782788535638105
1113	23	0.8254219924598942
1113	24	0.6528583824908338
1113	25	1.113879423182773
1114	1	-0.882259759071168
1114	2	-0.564394773335053
1114	3	-0.5498273471633617
1114	4	-0.2997823482449107
1114	5	-3.4552655001077284
1114	6	-1.1323997780073043
1114	7	-0.8329326514494113
1114	8	-0.6422397708987475
1114	9	-0.24949781060328036
1114	10	2.582263153954045E-4
1114	11	-0.4242697068754353
1114	12	-0.6018805922474684
1114	13	-0.5905541944343753
1114	14	-5.875352200546324
1114	15	-2.2320203486624237
1114	16	-1.169283026106804
1114	17	-0.25950306006605217
1114	18	-6.398197501456732
1114	19	-2.3545533520284962
1114	20	-1.299421629527842
1114	21	-0.9496933207603888
1114	22	-0.6211193258374621
1114	23	-0.2786752392911609
1114	24	-0.3581780094321019
1114	25	-0.24142021128994884
1115	1	-0.8598370685036869
1115	2	-0.49412652090217435
1115	3	-0.5685050145235344
1115	4	-2.9606979208634954
1115	5	-1.134600038433959
1115	6	-0.8802338337999132
1115	7	-0.7857727368311345
1115	8	-0.6489738757309098
1115	9	-0.5785771342459824
1115	10	-0.9259400284982462
1115	11	-0.8255154149900505
1115	12	-0.8949546948709113
1115	13	-0.9085486563506606
1115	14	-6.43567158470875
1115	15	-2.75252280147226
1115	16	-1.5617671927702332
1115	17	-0.6058554592579668
1115	18	-0.46197360955905964
1115	19	-0.7515036137916482
1115	20	-0.7785388473668391
1115	21	-0.9504167974590203
1115	22	-2.0323315682989076
1115	23	-1.169930180011768
1115	24	-1.0195639991421217
1115	25	-0.7026002570959591
1116	1	-0.4795571735678976
1116	2	-0.12278599822414549
1116	3	-0.2363023960506574
1116	4	-5.510493757299173E-6
1116	5	-0.6846349529695885
1116	6	-0.4169081714989318
1116	7	-0.47044326169083206
1116	8	-0.3332823528863287
1116	9	-0.4668983198231942
1116	10	-0.20827546295939925
1116	11	-0.43684117425412694
1116	12	-0.9301960899822461
1116	13	-0.749673052970401
1116	14	0.17895404779953594
1116	15	-0.3252400437791394
1116	16	-0.25597763553972186
1116	17	0.19469769958870842
1116	18	-0.17732591794550956
1116	19	-0.2968999387337661
1116	20	-0.6805865672873637
1116	21	-0.8783208227570043
1116	22	-0.778164696367611
1116	23	-1.4116482410312727
1116	24	-1.0264413416024993
1116	25	-0.4955253914172175
1201	1	1.1227912042510972
1201	2	1.293039494144022
1201	3	1.2819051119760019
1201	4	1.564727847984237
1201	5	1.4960328175052808
1201	6	1.2177669311116972
1201	7	1.134843328135446
1201	8	1.1970271669875459
1201	9	1.3887667337492502
1201	10	1.8305952675844637
1201	11	1.3917942771063139
1201	12	1.3578564495833092
1201	13	1.264092585961329
1201	14	2.170345196207556
1201	15	1.8384085019855192
1201	16	1.9729471925648312
1201	17	2.3692613437508996
1201	18	1.7994533182837529
1201	19	1.7347287628364967
1201	20	1.7134878761433825
1201	21	1.682143422046103
1201	22	1.9082642771059852
1201	23	2.1674483227036063
1201	24	1.7429111467300444
1201	25	2.101663763743325
1202	1	0.7305349913023349
1202	2	0.8667710301840579
1202	3	0.8748935156674484
1202	4	1.2100339887363418
1202	5	1.0468425860275374
1202	6	0.6971495375457195
1202	7	0.635615518519387
1202	8	0.7062204477652969
1202	9	0.9224480460244777
1202	10	1.2127970415595826
1202	11	0.8668217656490214
1202	12	0.8419098661445972
1202	13	0.8283278842994619
1202	14	1.676343528061082
1202	15	1.252240485555177
1202	16	1.379868027355549
1202	17	1.751884997350416
1202	18	1.9228039608788876
1202	19	1.419810690352847
1202	20	1.176062023460446
1202	21	1.1302190446862568
1202	22	1.3022209232347208
1202	23	1.4722150960957734
1202	24	1.177031020094411
1202	25	1.6001859294638123
1203	1	0.6615938382161737
1203	2	0.885729604669308
1203	3	0.7058258528241339
1203	4	1.0606126364148523
1203	5	1.1005632824158127
1203	6	0.7402642264743662
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2211	13	-0.7828659044727999
2211	14	-0.20049873281705288
2211	15	-0.41400545584771464
2211	16	-6.655466871925537
2211	17	-2.0074794249736456
2211	18	-0.8611781457041161
2211	19	-0.914430673664306
2211	20	-1.108684550344421
2211	21	-1.0271562954693536
2211	22	-0.8078510775099303
2211	23	-0.2526084610147805
2211	24	-0.6341414148448408
2211	25	-8.285152966816785
2212	1	-1.4645851356182256
2212	2	-4.096455273519471
2212	3	-1.8127849663711224
2212	4	-3.824603034881161
2212	5	-1.371488217899799
2212	6	-1.1867487670901085
2212	7	-1.0574772166557764
2212	8	-0.9123452333600355
2212	9	-1.0912054491908378
2212	10	-0.5500848391531008
2212	11	-1.0191625705739256
2212	12	-1.1598910709274932
2212	13	-1.301782111754136
2212	14	-6.605755110827147
2212	15	-5.1844068198990705
2212	16	-2.770234923441542
2212	17	-7.670600706870644
2212	18	-3.0420796202302895
2212	19	-1.8907974000383234
2212	20	-1.8753374134487544
2212	21	-1.4765150551497968
2212	22	-1.493408475084486
2212	23	-0.7695277838249837
2212	24	-1.2621379574693634
2212	25	-1.0045552633034092
2213	1	-0.41740419524745676
2213	2	-0.34323787620510515
2213	3	-0.4138563085485565
2213	4	-0.03884833755000727
2213	5	-0.17689778491575936
2213	6	-0.5015232542166501
2213	7	-0.48789266129592335
2213	8	-0.5628609441314509
2213	9	-0.3141650260547735
2213	10	-4.132335226132103
2213	11	-1.8828690107693618
2213	12	-1.064769300717387
2213	13	-0.9983756579433845
2213	14	-0.15352381361613254
2213	15	-0.7343346171808065
2213	16	-0.4154478369548258
2213	17	0.11631912913372844
2213	18	0.1006539272846112
2213	19	-0.5590511668636751
2213	20	-0.9021270107432144
2213	21	-0.9078849881892594
2213	22	-1.2065829643260244
2213	23	-1.4286067917462262
2213	24	-1.000068622087504
2213	25	-0.642228483261384
2214	1	-0.9250253714609329
2214	2	-0.6926849799886163
2214	3	-0.6306495049647793
2214	4	-0.20609269410942233
2214	5	-3.54532518035251
2214	6	-1.3121422263635196
2214	7	-1.0859835758647094
2214	8	-0.6954466711924141
2214	9	-0.6074122089642913
2214	10	-4.556603582343612
2214	11	-1.8663724171127498
2214	12	-1.3761806385863693
2214	13	-1.0172759759400165
2214	14	-0.369436286318674
2214	15	-0.5398734194223493
2214	16	-0.5177242852473469
2214	17	-0.19291605920147248
2214	18	0.18042976886454198
2214	19	-0.6519745670266062
2214	20	-0.9327026533840623
2214	21	-0.8765232450436358
2214	22	-0.6393033751326413
2214	23	-0.305046294531202
2214	24	-0.8290405349339025
2214	25	-0.6533029919900315
2215	1	-2.288207976147916
2215	2	-4.840909085638703
2215	3	-2.9254951001743628
2215	4	-1.922050693957555
2215	5	-1.9560992104952426
2215	6	-2.020423772419864
2215	7	-2.153076522475775
2215	8	-2.339280117378653
2215	9	-7.79464978102877
2215	10	-3.664538526949837
2215	11	-2.979629765319597
2215	12	-3.2749624113220364
2215	13	-2.76691982655003
2215	14	-8.036387069721524
2215	15	-4.630680483190456
2215	16	-9.44086170683629
2215	17	-4.582001034069048
2215	18	-3.0213080011231206
2215	19	-3.5083731020805757
2215	20	-3.2673268082790514
2215	21	-3.27305345629798
2215	22	-9.35013756852942
2215	23	-10.686223328613082
2215	24	-6.462000742265737
2215	25	-4.792632551378439
2216	1	-1.8239992618105987
2216	2	-1.852646731974822
2216	3	-1.7859247410898966
2216	4	-6.866139381041091
2216	5	-3.478892153006697
2216	6	-2.7799583630139573
2216	7	-2.153872094826095
2216	8	-8.035739724042589
2216	9	-3.9829057654207034
2216	10	-2.179155754657174
2216	11	-2.153220752464563
2216	12	-2.340855546671577
2216	13	-2.1346292938861424
2216	14	-1.4187009424148656
2216	15	-2.0400203500559115
2216	16	-1.6544466934566273
2216	17	-1.475481916615479
2216	18	-7.436941798156212
2216	19	-4.552278337370701
2216	20	-4.107530323279111
2216	21	-3.291062572737495
2216	22	-2.9213002707433304
2216	23	-2.1671488676336708
2216	24	-2.66579079430101
2216	25	-1.9744064606615694
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81569.0
3	1.0
4	667.0
5	4126.0
6	5059.0
7	4544.0
8	4186.0
9	3932.0
10	3691.0
11	3475.0
12	3462.0
13	3465.0
14	3536.0
15	3613.0
16	3615.0
17	3775.0
18	4428.0
19	5445.0
20	7043.0
21	9042.0
22	10836.0
23	13097.0
24	15157.0
25	17449.0
26	19585.0
27	22315.0
28	24783.0
29	30026.0
30	35977.0
31	42557.0
32	54673.0
33	71831.0
34	95875.0
35	129412.0
36	185749.0
37	273914.0
38	775687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79198387251997	18.414502575635552	12.211956824547809	25.58155672729667
2	14.660990609255062	20.250159588411474	40.94326258035828	24.145587221975184
3	18.3382464637123	24.913069535245246	29.744821438893133	27.003862562149322
4	11.406909019405798	16.791887498901236	39.09115436382787	32.71004911786509
5	12.763449359467394	37.84240367927891	36.26458481105974	13.129562150193957
6	31.773410350832204	37.29775193669069	18.152773626311376	12.776064086165729
7	28.02746885654158	31.519693549684124	22.526667479254975	17.92617011451932
8	25.256810387424473	36.01040239485707	20.938916275590636	17.79387094212783
9	26.69970675614027	14.841487481754445	19.970068693309813	38.48873706879547
10	13.935559247710739	28.483257593574642	35.72195542607724	21.859227732637386
11	34.42494351778374	22.38395161571761	24.487715023131535	18.703389843367113
12	23.063001728340485	24.70459641454072	31.633284286972298	20.599117570146497
13	28.985888435740257	21.58565040204225	25.745119828672387	23.683341333545098
14	20.66945124177955	21.30311236458579	28.71145455777757	29.315981835857087
15	23.483001635513663	29.725220551521716	23.448449954951286	23.343327858013335
16	22.550403052455994	27.68012939759109	27.29149154191715	22.47797600803576
17	21.76144624390797	27.866935928553865	28.048141354396737	22.32347647314143
18	21.718343044415363	27.196374619300123	29.2040116320536	21.88127070423091
19	23.465430618197892	26.965539113013154	27.45839388220751	22.110636386581444
20	23.82718172575481	26.907318701662632	27.433421514524603	21.83207805805796
21	23.53772246331249	26.56063865680543	26.8615645007661	23.04007437911598
22	23.393444165525867	27.24176169965629	26.968677814640895	22.396116320176944
23	22.597293156411087	27.05993811801684	27.526556799253814	22.816211926318257
24	23.289624193133047	26.822005365706325	27.417514786553056	22.470855654607572
25	23.04335333509373	26.71100036118505	27.434736289385103	22.810910014336113
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	239.0
1	239.0
2	387.5
3	536.0
4	536.0
5	536.0
6	995.5
7	1455.0
8	1455.0
9	1455.0
10	2297.0
11	3139.0
12	3139.0
13	3139.0
14	5678.0
15	8217.0
16	8217.0
17	8217.0
18	15355.5
19	22494.0
20	22494.0
21	22494.0
22	35186.5
23	47879.0
24	47879.0
25	47879.0
26	67727.0
27	87575.0
28	87575.0
29	87575.0
30	112402.5
31	137230.0
32	137230.0
33	137230.0
34	159444.5
35	181659.0
36	181659.0
37	181659.0
38	200310.0
39	218961.0
40	218961.0
41	218961.0
42	232382.0
43	245803.0
44	245803.0
45	245803.0
46	253313.5
47	260824.0
48	260824.0
49	260824.0
50	253268.0
51	245712.0
52	245712.0
53	245712.0
54	221131.0
55	196550.0
56	196550.0
57	196550.0
58	168958.5
59	141367.0
60	141367.0
61	141367.0
62	115861.0
63	90355.0
64	90355.0
65	90355.0
66	69933.5
67	49512.0
68	49512.0
69	49512.0
70	36520.5
71	23529.0
72	23529.0
73	23529.0
74	16397.0
75	9265.0
76	9265.0
77	9265.0
78	6381.5
79	3498.0
80	3498.0
81	3498.0
82	2374.0
83	1250.0
84	1250.0
85	1250.0
86	822.5
87	395.0
88	395.0
89	395.0
90	255.5
91	116.0
92	116.0
93	116.0
94	70.5
95	25.0
96	25.0
97	25.0
98	18.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16823447851104142
2	0.26982241579047705
3	0.26956958369172285
4	0.4790662607194489
5	0.2656759693709082
6	0.019417505184322184
7	0.019012973826315473
8	0.01820391111030205
9	0.020378267159588126
10	0.02184469333236246
11	0.021541294813857425
12	0.0281149293814665
13	0.028620593578974885
14	0.026041706171682097
15	0.042880323948711496
16	0.021996392591614976
17	0.04222296049195059
18	0.03266590715904201
19	0.02750813234445643
20	0.030693816788759286
21	0.03529536098608564
22	0.0462682740720177
23	0.030744383208510126
24	0.049049427158313855
25	0.03898670962789689
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1977597.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.50907663726409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.96105996396705	60.98390914799915
2	11.636310207412496	17.107488376233267
3	2.876911791613105	6.344373881050093
4	1.0816863241416124	3.180550515952251
5	0.49393286867885927	1.8154274548688984
6	0.25162848043775904	1.109818635157052
7	0.15440699001158856	0.7945220683463794
8	0.10751717406691831	0.6322790550645728
9	0.07631613758117596	0.5048935925503188
>10	0.33442267505019696	4.185642236239665
>50	0.015232401293467602	0.7683928308388606
>100	0.00876719071484927	1.2648155094511755
>500	0.001112489249839922	0.5106528579552637
>1k	6.953057811499084E-4	0.797233838293044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2478	0.1253035881425791	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.056641975083902E-5	0.0
4	0.0	0.0	0.0	5.056641975083902E-5	0.0
5	0.0	0.0	0.0	5.056641975083902E-5	0.0
6	0.0	0.0	0.0	5.056641975083902E-5	0.0
7	0.0	0.0	0.0	5.056641975083902E-5	0.0
8	0.0	0.0	0.0	5.056641975083902E-5	0.0
9	0.0	0.0	0.0	5.056641975083902E-5	0.0
10	0.0	0.0	0.0	5.056641975083902E-5	0.0
11	0.0	0.0	0.0	5.056641975083902E-5	0.0
12	0.0	0.0	0.0	5.056641975083902E-5	5.056641975083902E-5
13	0.0	0.0	0.0	5.056641975083902E-5	5.056641975083902E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATACG	40	0.005171219	14.294688	4
TAGGACC	580	0.0	13.473154	4
GACCGAC	55	0.0030996935	12.074828	7
GTAGGAC	1150	0.0	11.601781	3
GGCGAGG	440	0.0	10.999779	19
AGGACGT	935	0.0	10.985344	5
GTCCTAC	1025	0.0	10.903398	1
TGTAGGA	1260	0.0	10.8995	2
GGACGTG	915	0.0	10.88714	6
CGAAATC	80	3.8138582E-4	10.674635	13
AGGACCT	955	0.0	10.655699	5
TCCTACA	1100	0.0	10.404067	2
TTAGGAC	770	0.0	10.3964	3
TAGGACG	990	0.0	10.299876	4
TGTACTG	370	0.0	10.281578	5
TGGCGAG	960	0.0	10.280579	18
GACGTGA	555	0.0	10.085667	7
ACGAAAT	105	4.160431E-5	9.940401	12
TATGGCG	1005	0.0	9.914931	16
AGGCGTG	135	6.5619497E-7	9.838748	7
>>END_MODULE