FastQCFastQC Report
Thu 26 May 2016
SRR1513168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977597
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34090.17238092493061022No Hit
TATCAACGCAGAGTACTTTTTTTTT22940.11599936690842472No Hit
GTACATGGGAAGCAGTGGTATCAAC21320.10780760690878881No Hit
GGTATCAACGCAGAGTACTTTTTTT20280.10254869925470154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG500.001475113913.3293214
TAGGACC4400.012.5503344
GGCGAGG4250.011.83354719
ATACGGG657.884913E-411.7168956
TGGCGAG9350.010.55639618
TTAGTAC1451.3938916E-810.5064023
GTCCTAG2400.010.3195911
TCTATAC1951.4551915E-1110.2538433
TGGACCG750.00260017310.1556735
GTCCTAA6150.09.9130031
GCCTAAG1451.5546539E-79.8542521
GTATAAG1752.4556357E-99.7979421
GTAGGAC10000.09.7118553
TGTAGGA10750.09.5669262
GTAGGGG1701.6047125E-89.5214263
TAGTACC1304.12169E-69.5209454
AAGACGG900.0010915839.5209445
AGGACCT8600.09.5209435
AAGTCGT1002.7796743E-49.4918047
GTCCTAC11850.09.4051971
Produced by FastQC (version 0.11.5)