##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513168_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1977597 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.869480485660123 31.0 31.0 34.0 28.0 34.0 2 30.974601498687548 33.0 31.0 34.0 27.0 34.0 3 31.071904437557297 33.0 31.0 34.0 27.0 34.0 4 34.46091948966347 37.0 35.0 37.0 31.0 37.0 5 34.205734535398264 37.0 35.0 37.0 30.0 37.0 6 34.11604942766398 37.0 35.0 37.0 30.0 37.0 7 33.98828527753632 37.0 35.0 37.0 30.0 37.0 8 33.95753583768584 37.0 35.0 37.0 30.0 37.0 9 35.527303085512365 39.0 37.0 39.0 30.0 39.0 10 35.374796786200626 39.0 35.0 39.0 29.0 39.0 11 35.385236729222385 39.0 37.0 39.0 30.0 39.0 12 35.325954175699096 39.0 35.0 39.0 29.0 39.0 13 35.290303838446356 39.0 35.0 39.0 29.0 39.0 14 36.47047401467539 40.0 37.0 41.0 29.0 41.0 15 36.45496377674521 40.0 37.0 41.0 29.0 41.0 16 36.41537279840129 40.0 37.0 41.0 29.0 41.0 17 36.35713140746067 40.0 37.0 41.0 28.0 41.0 18 36.314708709610706 40.0 37.0 41.0 28.0 41.0 19 36.39625818607128 40.0 37.0 41.0 28.0 41.0 20 36.365886983040525 40.0 37.0 41.0 28.0 41.0 21 36.31382733691445 40.0 37.0 41.0 28.0 41.0 22 36.25754488907497 40.0 37.0 41.0 28.0 41.0 23 36.18060504743889 40.0 37.0 41.0 28.0 41.0 24 36.11698237810838 40.0 37.0 41.0 28.0 41.0 25 36.05900039290108 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4144669077406604 1101 2 0.5140394321113426 1101 3 0.39015660013805586 1101 4 0.5325253268033379 1101 5 0.4163410100511129 1101 6 0.31241255380927413 1101 7 0.408881594425786 1101 8 0.5574442672082327 1101 9 0.5365449709539263 1101 10 0.626903556188104 1101 11 0.6401771966905585 1101 12 0.5817459492291661 1101 13 0.6130442896348711 1101 14 0.7114176208764889 1101 15 0.7243430526711379 1101 16 0.5941718314307209 1101 17 0.7306421300388592 1101 18 0.5926300882341451 1101 19 0.6350656450119132 1101 20 0.6796625910521996 1101 21 0.7105032115211642 1101 22 0.6808682156403236 1101 23 0.7553264996404749 1101 24 0.7417171723071831 1101 25 0.717838815392426 1102 1 0.49870760972159545 1102 2 0.49080768131887353 1102 3 0.48865266061933355 1102 4 0.5501198216945582 1102 5 0.5737060438680999 1102 6 0.6318250970866544 1102 7 0.6394882072921675 1102 8 0.6332014105472155 1102 9 0.6261655779979733 1102 10 0.6892401560115076 1102 11 0.7930183907144297 1102 12 0.8060815040821439 1102 13 0.871185588958852 1102 14 0.8330127414156436 1102 15 0.7674595107823166 1102 16 0.8972855294757807 1102 17 0.9544554467772031 1102 18 0.8201631932751638 1102 19 0.8939246356554804 1102 20 0.8509149333710653 1102 21 0.7803116340222971 1102 22 0.8183416234410785 1102 23 0.7762936124766497 1102 24 0.9208805510481781 1102 25 0.8496073864429405 1103 1 0.2987027630608523 1103 2 0.2469171698083592 1103 3 0.33205293239583256 1103 4 0.3759326521383741 1103 5 0.35075807458865427 1103 6 0.21459594514929137 1103 7 0.2422469265862972 1103 8 0.1985519059062355 1103 9 0.28240210958215073 1103 10 0.22907637522839508 1103 11 0.2303483410785674 1103 12 0.2236181766435621 1103 13 0.2854518560183408 1103 14 0.4043050005108526 1103 15 0.31179331000091537 1103 16 0.3029421883938497 1103 17 0.38297416791306205 1103 18 0.3244886562372855 1103 19 0.3824223586336828 1103 20 0.2746039570779004 1103 21 0.2625582551424728 1103 22 0.3907017230641898 1103 23 0.31609311823842035 1103 24 0.4312104208365781 1103 25 0.36793121729206035 1104 1 0.47341846046814595 1104 2 0.5079908658968009 1104 3 0.5082161507702203 1104 4 0.5975583368570909 1104 5 0.5618205818792319 1104 6 0.6445185996082614 1104 7 0.6073776331707919 1104 8 0.6761458620509089 1104 9 0.6868595362062493 1104 10 0.7781731773747396 1104 11 0.7355463708979073 1104 12 0.5847065133930514 1104 13 0.662261286984652 1104 14 0.8083045196306031 1104 15 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-0.9527982675306887 2215 22 -1.0079121337640942 2215 23 -0.9192970094555406 2215 24 -1.008269084959906 2215 25 -1.0143650234978878 2216 1 -0.32152084347551835 2216 2 -0.31911840104648803 2216 3 -0.38774926406222576 2216 4 -0.3269717865008843 2216 5 -0.3905054566938304 2216 6 -0.43566174502645083 2216 7 -0.6570346085462546 2216 8 -0.5979968222430116 2216 9 -0.6544683114299161 2216 10 -0.7390193579581634 2216 11 -0.5638067661766542 2216 12 -0.5381816453177208 2216 13 -0.601544813279105 2216 14 -0.7249846987745627 2216 15 -0.6304658402088208 2216 16 -0.7108822172781544 2216 17 -0.85096327390157 2216 18 -0.7761241891495771 2216 19 -0.7810235934123568 2216 20 -0.8214636588050155 2216 21 -0.9613465009376867 2216 22 -0.877178888092061 2216 23 -0.9207378896218827 2216 24 -0.9537566652986342 2216 25 -1.0567344261858906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44749.0 3 1.0 4 810.0 5 6539.0 6 7318.0 7 5655.0 8 4587.0 9 3927.0 10 3588.0 11 3344.0 12 2987.0 13 2933.0 14 2752.0 15 2671.0 16 2801.0 17 2797.0 18 3309.0 19 3891.0 20 5020.0 21 6421.0 22 8322.0 23 10077.0 24 11687.0 25 13007.0 26 14168.0 27 15675.0 28 17589.0 29 21391.0 30 27048.0 31 34758.0 32 44741.0 33 58139.0 34 77507.0 35 104622.0 36 150111.0 37 246630.0 38 1006025.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.36634690594009 18.996735522620337 13.092307099985003 27.54461047145457 2 14.057111011766896 20.445410465800755 39.50031657540259 25.997161947029756 3 16.879757631046772 24.855004927986666 29.95270245327262 28.312534987693937 4 11.244924274986726 17.17003362746833 37.73826199084726 33.84678010669768 5 12.824112404175683 38.05154017283296 35.93161377562485 13.192733647366515 6 31.87736882343004 37.45428497361224 17.88807612094082 12.780270082016903 7 27.755398507254327 31.56131324391966 22.55451111466302 18.128777134162995 8 25.44479576313703 36.17254130860481 20.52640149860012 17.85626142965804 9 26.4226949522553 14.658479886442304 19.661015920630977 39.25780924067141 10 14.406507962514588 28.716581812738813 34.899136087900175 21.977774136846428 11 34.415707548100045 22.224143746172754 24.440823888790284 18.919324816936918 12 22.790335948122898 24.752869265072714 31.67510873044407 20.78168605636032 13 28.899467383900763 21.502308104229527 25.795599406754764 23.802625105114945 14 21.248190957975613 21.363686471146853 27.56216480259066 29.825957768286877 15 23.41847201426782 29.693966971025947 23.33407665970367 23.55348435500256 16 22.80125829478908 27.75004209654444 26.686478589925045 22.762221018741432 17 22.065349441074336 28.063065386868004 27.297988150836595 22.57359702122107 18 22.140218602507925 27.337857667096777 28.52535618126942 21.996567549125878 19 23.47672452982079 26.9279332442353 27.367608263968847 22.227733961975062 20 23.75777269079595 26.82503058004234 27.470005263964296 21.947191465197406 21 23.374481185122857 26.49015161934967 26.96593419040607 23.1694330051214 22 23.382998035492605 27.21310480659589 26.76372057979515 22.640176578116346 23 22.791445635079167 26.95363267205065 27.136947716020977 23.117973976849207 24 23.13287287551508 26.768244490662152 27.472533584951837 22.626349048870928 25 23.026539346842018 26.780599840614244 27.17896310260155 23.013897709942192 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 292.0 3 399.0 4 399.0 5 399.0 6 876.0 7 1353.0 8 1353.0 9 1353.0 10 2055.5 11 2758.0 12 2758.0 13 2758.0 14 5058.5 15 7359.0 16 7359.0 17 7359.0 18 14406.5 19 21454.0 20 21454.0 21 21454.0 22 33703.5 23 45953.0 24 45953.0 25 45953.0 26 65660.5 27 85368.0 28 85368.0 29 85368.0 30 109833.0 31 134298.0 32 134298.0 33 134298.0 34 156152.0 35 178006.0 36 178006.0 37 178006.0 38 196363.0 39 214720.0 40 214720.0 41 214720.0 42 228625.5 43 242531.0 44 242531.0 45 242531.0 46 253026.5 47 263522.0 48 263522.0 49 263522.0 50 256400.5 51 249279.0 52 249279.0 53 249279.0 54 224991.5 55 200704.0 56 200704.0 57 200704.0 58 172822.0 59 144940.0 60 144940.0 61 144940.0 62 119451.0 63 93962.0 64 93962.0 65 93962.0 66 72660.0 67 51358.0 68 51358.0 69 51358.0 70 37851.5 71 24345.0 72 24345.0 73 24345.0 74 16946.0 75 9547.0 76 9547.0 77 9547.0 78 6622.0 79 3697.0 80 3697.0 81 3697.0 82 2509.0 83 1321.0 84 1321.0 85 1321.0 86 861.5 87 402.0 88 402.0 89 402.0 90 264.5 91 127.0 92 127.0 93 127.0 94 68.0 95 9.0 96 9.0 97 9.0 98 4.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1831010059177881 2 0.009354787653905218 3 0.005865704691097326 4 0.002376621728289434 5 0.08014777530507985 6 0.3245352819608849 7 0.028772292838227402 8 0.014411429628989121 9 0.005056641975083902 10 0.004095879999817961 11 0.0 12 0.0 13 0.0 14 0.0015675590122760097 15 0.0 16 0.0 17 0.02078279851759484 18 0.029530789134489987 19 0.0 20 0.0 21 6.573634567609073E-4 22 1.0113283950167805E-4 23 0.006421935308356557 24 0.0 25 2.528320987541951E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1977597.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.78196720588171 #Duplication Level Percentage of deduplicated Percentage of total 1 81.29210339669667 57.540149967221296 2 12.506807975837797 17.70512943992022 3 3.322813025966656 7.055857279057454 4 1.1986041321685432 3.3935823350395258 5 0.5511724078329271 1.9506533648008553 6 0.3020521897508161 1.2827908913644217 7 0.18666156176535456 0.9248590780431795 8 0.122030705301143 0.6910058704588896 9 0.09131640747287716 0.5817199463193711 >10 0.39323830685048533 4.723774150512627 >50 0.019772119513524616 0.9435231806196342 >100 0.011215070477970728 1.5093024953340175 >500 0.0014275486227195886 0.6695727032123674 >1k 7.851517424957492E-4 1.0280792980961386 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3409 0.17238092493061022 No Hit TATCAACGCAGAGTACTTTTTTTTT 2294 0.11599936690842472 No Hit GTACATGGGAAGCAGTGGTATCAAC 2132 0.10780760690878881 No Hit GGTATCAACGCAGAGTACTTTTTTT 2028 0.10254869925470154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 5.056641975083902E-5 0.0 0.0 0.0 0.0 4 5.056641975083902E-5 0.0 0.0 0.0 0.0 5 5.056641975083902E-5 0.0 0.0 0.0 0.0 6 5.056641975083902E-5 0.0 0.0 0.0 0.0 7 5.056641975083902E-5 0.0 0.0 0.0 0.0 8 5.056641975083902E-5 0.0 0.0 0.0 0.0 9 5.056641975083902E-5 0.0 0.0 0.0 0.0 10 5.056641975083902E-5 0.0 0.0 0.0 0.0 11 5.056641975083902E-5 0.0 0.0 0.0 0.0 12 5.056641975083902E-5 5.056641975083902E-5 0.0 0.0 0.0 13 5.056641975083902E-5 5.056641975083902E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCACG 50 0.0014751139 13.329321 4 TAGGACC 440 0.0 12.550334 4 GGCGAGG 425 0.0 11.833547 19 ATACGGG 65 7.884913E-4 11.716895 6 TGGCGAG 935 0.0 10.556396 18 TTAGTAC 145 1.3938916E-8 10.506402 3 GTCCTAG 240 0.0 10.319591 1 TCTATAC 195 1.4551915E-11 10.253843 3 TGGACCG 75 0.002600173 10.155673 5 GTCCTAA 615 0.0 9.913003 1 GCCTAAG 145 1.5546539E-7 9.854252 1 GTATAAG 175 2.4556357E-9 9.797942 1 GTAGGAC 1000 0.0 9.711855 3 TGTAGGA 1075 0.0 9.566926 2 GTAGGGG 170 1.6047125E-8 9.521426 3 TAGTACC 130 4.12169E-6 9.520945 4 AAGACGG 90 0.001091583 9.520944 5 AGGACCT 860 0.0 9.520943 5 AAGTCGT 100 2.7796743E-4 9.491804 7 GTCCTAC 1185 0.0 9.405197 1 >>END_MODULE