FastQCFastQC Report
Thu 26 May 2016
SRR1513167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754411
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC350.002087908616.3808671
ATTAGAC1552.3646862E-1111.6856273
TAGGACC4700.010.9530224
GTCCTAC10300.010.6687681
GTAGGAC7550.010.483
CGCAGAT1201.5526584E-610.2771678
TCCTACA12000.010.2554732
TAGGCCG856.5224007E-410.0711715
GGACCGA951.6690436E-49.9855496
AGGACCG1856.348273E-109.7687345
TAGGACT3800.09.5331864
CTAAGAC2600.09.5330113
ACAGCGT1701.7049388E-89.4866168
TTATACT3150.09.3818654
TAGACTG3150.09.36068255
GTCCTAA6600.09.26594451
TTAGGAC5700.09.198523
TCTACGC950.00176769259.0312753
CCTACAG12050.09.0187833
CTAGCAC2750.09.0130293
Produced by FastQC (version 0.11.5)