FastQCFastQC Report
Thu 26 May 2016
SRR1513167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754411
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34210.124200781945759No Hit
GTACATGGGAAGCAGTGGTATCAAC28260.10259906745943143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG550.00302042412.1169445
ACCGTCC752.0977271E-411.386598
GGACGTG7600.010.8974256
TAGGACC5900.010.8113654
GACGTGA5050.010.7122987
GTAGGAC9150.010.6137423
TAGACAG4400.010.6023265
AGGACGT8000.010.5915095
TTAGGAC6500.010.5465053
TAGGACG7500.010.5359724
TAGACTG2650.010.41861255
ACACCGT1951.4551915E-1110.2518666
GGCGAGG5300.010.20385219
GTCCTAA4800.010.12101651
ATTGCGA750.002676680510.12049510
TGGCGAG9500.010.09031818
GTACTAA1701.4897523E-910.0859961
CAGGACT6450.010.037074
GTCCTAT2002.3646862E-1110.00194551
GTCCTAC9000.09.7373441
Produced by FastQC (version 0.11.5)