##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513167_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2754411 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.73156221057787 31.0 31.0 34.0 27.0 34.0 2 30.83518908398202 33.0 31.0 34.0 27.0 34.0 3 30.941408526178556 33.0 31.0 34.0 27.0 34.0 4 34.31812028052458 37.0 35.0 37.0 31.0 37.0 5 34.04757968218977 37.0 35.0 37.0 30.0 37.0 6 33.96006296809009 37.0 35.0 37.0 30.0 37.0 7 33.819484456023446 37.0 35.0 37.0 29.0 37.0 8 33.78897993073655 37.0 35.0 37.0 29.0 37.0 9 35.35398203100409 39.0 35.0 39.0 29.0 39.0 10 35.19834222271113 39.0 35.0 39.0 28.0 39.0 11 35.21127565929703 39.0 35.0 39.0 29.0 39.0 12 35.14097678233205 39.0 35.0 39.0 28.0 39.0 13 35.1119393583601 39.0 35.0 39.0 28.0 39.0 14 36.27196413316676 40.0 37.0 41.0 27.0 41.0 15 36.248602695821354 40.0 37.0 41.0 27.0 41.0 16 36.21009028790547 40.0 36.0 41.0 27.0 41.0 17 36.136158692366536 40.0 36.0 41.0 27.0 41.0 18 36.09057508120611 40.0 36.0 41.0 27.0 41.0 19 36.16750877047761 40.0 37.0 41.0 27.0 41.0 20 36.12686523543509 40.0 37.0 41.0 27.0 41.0 21 36.07201212890887 40.0 37.0 41.0 27.0 41.0 22 36.01379351157107 40.0 37.0 41.0 27.0 41.0 23 35.93570494744611 40.0 37.0 41.0 27.0 41.0 24 35.86509384401965 40.0 36.0 41.0 27.0 41.0 25 35.80980216823125 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5535236108009656 1101 2 0.5701170754713338 1101 3 0.5365534160502534 1101 4 0.5911942865551154 1101 5 0.5524402731139233 1101 6 0.6386112880892298 1101 7 0.6568860188949159 1101 8 0.5964818609008589 1101 9 0.6938388616220976 1101 10 0.8169869599176565 1101 11 0.6754607011760569 1101 12 0.705020018581969 1101 13 0.832781485481064 1101 14 0.9716157096770672 1101 15 1.0063813668649004 1101 16 0.9972999780690657 1101 17 0.9896399255181478 1101 18 0.9830914364447239 1101 19 1.0103887587938516 1101 20 1.0387968631691251 1101 21 1.025271813293223 1101 22 0.9380500117997599 1101 23 0.9571344860074049 1101 24 1.0100238848974854 1101 25 1.073197495376398 1102 1 0.3379544563159129 1102 2 0.4184975439883125 1102 3 0.3619451734946999 1102 4 0.48592388463611513 1102 5 0.4636386110184887 1102 6 0.3951420389217901 1102 7 0.3847610091248512 1102 8 0.3899717315828113 1102 9 0.35357312674948105 1102 10 0.5086966183013573 1102 11 0.5479709424648931 1102 12 0.4716556537362493 1102 13 0.4250728581046417 1102 14 0.5419942078914914 1102 15 0.5003548609981365 1102 16 0.554312887698373 1102 17 0.5703191884875594 1102 18 0.6234557138218193 1102 19 0.5191496514775196 1102 20 0.6007639325336598 1102 21 0.5297653597105167 1102 22 0.5376725963346232 1102 23 0.5318202320579388 1102 24 0.6380471474442047 1102 25 0.6323147850482442 1103 1 0.3915857758806247 1103 2 0.4178403617377171 1103 3 0.4836443533885024 1103 4 0.5464248062020332 1103 5 0.4743209037536218 1103 6 0.2975955988378871 1103 7 0.49283230445372794 1103 8 0.5021483287384427 1103 9 0.5093996656214372 1103 10 0.507063403125656 1103 11 0.5199490570634779 1103 12 0.5571036588124159 1103 13 0.6356697529284503 1103 14 0.7179365666867596 1103 15 0.7131328216578439 1103 16 0.6700338162297044 1103 17 0.6842120928275861 1103 18 0.6842329461343581 1103 19 0.6736383692525294 1103 20 0.6693446574140296 1103 21 0.6058358948866669 1103 22 0.6517082280962825 1103 23 0.6578533161947604 1103 24 0.6996219040124458 1103 25 0.7209930416586303 1104 1 0.2945184867433248 1104 2 0.3610558038453142 1104 3 0.39118204494624464 1104 4 0.5554086001626004 1104 5 0.537396300302234 1104 6 0.4561735830725553 1104 7 0.40579878930812896 1104 8 0.48822798823956504 1104 9 0.49326776673984796 1104 10 0.6293547543074922 1104 11 0.6137506745158063 1104 12 0.6125571742370113 1104 13 0.5676977217408492 1104 14 0.6800105749729326 1104 15 0.6091832485989173 1104 16 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-1.2920720009896414 2215 22 -1.3971740667233021 2215 23 -1.2508703140490667 2215 24 -1.3578285074386969 2215 25 -1.3802814813407949 2216 1 -0.517177953469254 2216 2 -0.4522452158966459 2216 3 -0.5128044798330791 2216 4 -0.37462819330735186 2216 5 -0.5757870557837492 2216 6 -0.6502785310182162 2216 7 -0.811518263492438 2216 8 -0.7516432098228236 2216 9 -0.8747727153136253 2216 10 -0.8449674035712391 2216 11 -0.7924954941476869 2216 12 -0.8946565548618182 2216 13 -0.8640035428566293 2216 14 -1.150168840226442 2216 15 -1.0662341866036726 2216 16 -1.0030546439106232 2216 17 -1.0646249125183758 2216 18 -1.0232789336189398 2216 19 -1.0588698782812216 2216 20 -1.045619578667143 2216 21 -1.2454180665791554 2216 22 -1.0607095434397493 2216 23 -1.1789642591720835 2216 24 -1.1655733764291227 2216 25 -1.298519505679991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69403.0 3 1.0 4 1166.0 5 9624.0 6 10590.0 7 8297.0 8 6616.0 9 5889.0 10 5108.0 11 4800.0 12 4584.0 13 4210.0 14 4156.0 15 4071.0 16 4148.0 17 4359.0 18 4783.0 19 5697.0 20 7370.0 21 9416.0 22 11921.0 23 14326.0 24 16379.0 25 18686.0 26 20501.0 27 22658.0 28 25533.0 29 30849.0 30 38855.0 31 49705.0 32 63328.0 33 82634.0 34 109909.0 35 148692.0 36 212597.0 37 347937.0 38 1365613.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.02493134233687 18.39247051566624 12.766961106902283 27.815637035094603 2 14.57005941583386 19.776606050219524 38.51176479985825 27.141569734088367 3 17.27328509238517 23.999811199500403 29.337564400939648 29.389339307174783 4 11.579839352647225 16.27523820450225 36.54843455239627 35.59648789045425 5 13.267775684567551 37.69184513958402 35.39873232604908 13.641646849799349 6 33.38280644770049 36.48049114237003 17.044768737168102 13.09193367276139 7 28.870446962898775 30.908593713968003 21.713891014366858 18.507068308766357 8 26.341527887919643 35.792861645359665 19.638209398320424 18.227401068400276 9 26.532549869910895 14.432375086502175 18.88201793145615 40.15305711213078 10 14.641417447535728 28.516179955509614 34.23406938161851 22.60833321533615 11 35.69634306572258 21.80952661022629 23.27481265504676 19.219317669004372 12 23.035523747182246 24.273864720987536 31.015814270274117 21.6747972615561 13 29.246978755167618 20.73641152318953 25.369852211598054 24.646757510044797 14 21.718129791708947 20.349829924625148 26.911482747215604 31.020557536450298 15 24.102212777976852 28.85727656475377 22.782075732343504 24.258434924925872 16 23.67700390391993 27.179930663942308 25.58114965413658 23.56191577800118 17 22.85719265793192 27.434927876785377 26.510723795849344 23.197155669433357 18 23.000833114825305 26.95173672118835 27.277283421014975 22.770146742971363 19 24.00128375903233 26.519862141125632 26.62398603548998 22.85486806435205 20 24.2118913989234 26.290085248715606 26.67913394188449 22.818889410476505 21 24.021696313333106 25.95337979258528 26.36407038231765 23.660853511763968 22 24.046710547812417 26.33344345976089 26.161972981509653 23.457873010917037 23 23.563558097417943 26.15923254216414 26.352208017032673 23.925001343385244 24 23.581811138570096 26.312848736081868 26.59624870798149 23.50909141736654 25 23.606896148608396 26.095489882214213 26.475426471106122 23.822187498071273 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 120.0 2 261.5 3 403.0 4 403.0 5 403.0 6 976.5 7 1550.0 8 1550.0 9 1550.0 10 2384.0 11 3218.0 12 3218.0 13 3218.0 14 5947.0 15 8676.0 16 8676.0 17 8676.0 18 16437.5 19 24199.0 20 24199.0 21 24199.0 22 38082.0 23 51965.0 24 51965.0 25 51965.0 26 75261.5 27 98558.0 28 98558.0 29 98558.0 30 127696.0 31 156834.0 32 156834.0 33 156834.0 34 187340.0 35 217846.0 36 217846.0 37 217846.0 38 247583.5 39 277321.0 40 277321.0 41 277321.0 42 301396.0 43 325471.0 44 325471.0 45 325471.0 46 346780.5 47 368090.0 48 368090.0 49 368090.0 50 366543.5 51 364997.0 52 364997.0 53 364997.0 54 336951.5 55 308906.0 56 308906.0 57 308906.0 58 271362.0 59 233818.0 60 233818.0 61 233818.0 62 194674.5 63 155531.0 64 155531.0 65 155531.0 66 121624.5 67 87718.0 68 87718.0 69 87718.0 70 64844.0 71 41970.0 72 41970.0 73 41970.0 74 29623.5 75 17277.0 76 17277.0 77 17277.0 78 12025.0 79 6773.0 80 6773.0 81 6773.0 82 4548.0 83 2323.0 84 2323.0 85 2323.0 86 1480.5 87 638.0 88 638.0 89 638.0 90 413.0 91 188.0 92 188.0 93 188.0 94 103.0 95 18.0 96 18.0 97 18.0 98 10.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17789647224034466 2 0.009548320856981764 3 0.006571277852143343 4 0.0019967971373916235 5 0.07718528571081076 6 0.31992320681263614 7 0.02839082475345909 8 0.014050190766737424 9 0.005264283362214281 10 0.003920983469787189 11 0.0 12 0.0 13 0.0 14 0.001597437709913299 15 0.0 16 0.0 17 0.021129744253853184 18 0.032275502820748245 19 0.0 20 0.0 21 9.439404649487676E-4 22 3.630540249802953E-5 23 0.006353445437155167 24 0.0 25 1.452216099921181E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2754411.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.85550251744694 #Duplication Level Percentage of deduplicated Percentage of total 1 80.04460172072673 54.314666735688085 2 12.640755625708872 17.154896503654395 3 3.635544074422777 7.400750102828521 4 1.4199276646662289 3.853996208974073 5 0.6942818126055532 2.355542064153687 6 0.395434224992294 1.6099432829669573 7 0.2514121188229747 1.1941786963196321 8 0.17693868117841965 0.9605010500908796 9 0.12424507146139828 0.7587640583396357 >10 0.5805894587845937 6.614337593519871 >50 0.02336140992673913 1.0637521185845427 >100 0.011087449714981323 1.3264802143164713 >500 0.0010709923461521868 0.5281126841422377 >1k 7.496946422697177E-4 0.864078686421069 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3421 0.124200781945759 No Hit GTACATGGGAAGCAGTGGTATCAAC 2826 0.10259906745943143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.630540249802953E-5 0.0 3.630540249802953E-5 3.630540249802953E-5 6 0.0 3.630540249802953E-5 0.0 3.630540249802953E-5 3.630540249802953E-5 7 0.0 7.261080499605905E-5 0.0 3.630540249802953E-5 3.630540249802953E-5 8 0.0 7.261080499605905E-5 0.0 3.630540249802953E-5 3.630540249802953E-5 9 0.0 7.261080499605905E-5 0.0 3.630540249802953E-5 3.630540249802953E-5 10 0.0 7.261080499605905E-5 0.0 3.630540249802953E-5 3.630540249802953E-5 11 0.0 7.261080499605905E-5 0.0 3.630540249802953E-5 3.630540249802953E-5 12 0.0 7.261080499605905E-5 0.0 3.630540249802953E-5 3.630540249802953E-5 13 0.0 7.261080499605905E-5 0.0 3.630540249802953E-5 1.0891620749408857E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCG 55 0.003020424 12.116944 5 ACCGTCC 75 2.0977271E-4 11.38659 8 GGACGTG 760 0.0 10.897425 6 TAGGACC 590 0.0 10.811365 4 GACGTGA 505 0.0 10.712298 7 GTAGGAC 915 0.0 10.613742 3 TAGACAG 440 0.0 10.602326 5 AGGACGT 800 0.0 10.591509 5 TTAGGAC 650 0.0 10.546505 3 TAGGACG 750 0.0 10.535972 4 TAGACTG 265 0.0 10.4186125 5 ACACCGT 195 1.4551915E-11 10.251866 6 GGCGAGG 530 0.0 10.203852 19 GTCCTAA 480 0.0 10.1210165 1 ATTGCGA 75 0.0026766805 10.120495 10 TGGCGAG 950 0.0 10.090318 18 GTACTAA 170 1.4897523E-9 10.085996 1 CAGGACT 645 0.0 10.03707 4 GTCCTAT 200 2.3646862E-11 10.0019455 1 GTCCTAC 900 0.0 9.737344 1 >>END_MODULE