FastQCFastQC Report
Thu 26 May 2016
SRR1513166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208726
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6400.013.0944394
ACCGTGC1103.8802682E-812.0765558
GTGCGCC1157.171184E-811.55436711
CTAGTAC1451.1677912E-911.1659263
CGTGCGC855.379851E-511.16522610
GTCCTAC13100.011.0750251
AGGACCT10600.011.0337355
GGACCTG10050.010.7628396
TCCTACA14100.010.7467772
GACCGTG1158.9569585E-710.7263817
GTCCTAT2850.010.3821951
GTCTTAT2850.010.3821951
AGAACCG1105.981703E-610.3731795
GTCCTAA7000.010.2267021
GGACGTG10100.010.1458976
GTAGGAC13050.010.0712273
TGGCGAG11450.010.0307918
CCTACAG14750.09.9435743
TGTAGGA14200.09.9328422
TAGACTG2500.09.8890985
Produced by FastQC (version 0.11.5)