##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513166_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2208726 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.046171865591294 31.0 30.0 34.0 23.0 34.0 2 28.92406572838822 31.0 30.0 34.0 16.0 34.0 3 29.146735267298887 31.0 30.0 34.0 22.0 34.0 4 32.2206557083133 35.0 33.0 37.0 19.0 37.0 5 32.3878172303853 35.0 33.0 37.0 22.0 37.0 6 32.73226737947577 37.0 33.0 37.0 25.0 37.0 7 32.66872939422998 37.0 33.0 37.0 25.0 37.0 8 32.576165173950955 37.0 33.0 37.0 24.0 37.0 9 33.75167585295777 39.0 34.0 39.0 21.0 39.0 10 33.323885352913855 38.0 33.0 39.0 17.0 39.0 11 33.78423036628355 38.0 34.0 39.0 23.0 39.0 12 33.76326760313412 38.0 34.0 39.0 23.0 39.0 13 33.81460262612927 38.0 34.0 39.0 23.0 39.0 14 34.020412219532886 39.0 33.0 41.0 12.0 41.0 15 34.426893602918604 39.0 34.0 41.0 17.0 41.0 16 34.26476711008971 39.0 33.0 41.0 17.0 41.0 17 33.889358843061565 39.0 33.0 41.0 11.0 41.0 18 33.595046194050326 39.0 32.0 41.0 10.0 41.0 19 34.223427441882784 39.0 34.0 41.0 18.0 41.0 20 34.4217997162165 39.0 34.0 41.0 18.0 41.0 21 34.46847277570871 39.0 34.0 41.0 18.0 41.0 22 34.158084343644255 39.0 34.0 41.0 10.0 41.0 23 33.684880786480534 39.0 33.0 41.0 8.0 41.0 24 33.91477349386026 39.0 34.0 41.0 2.0 41.0 25 33.531293605454 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0158927683131154 1101 2 1.3394453198077692 1101 3 1.2485364726191186 1101 4 1.6065115039241746 1101 5 1.5229263340150467 1101 6 1.1685539030027527 1101 7 1.168665202001904 1101 8 1.2401320640400684 1101 9 1.610154124990821 1101 10 1.9086839547910444 1101 11 1.4903699558298698 1101 12 1.4344138212023907 1101 13 1.3439044799444986 1101 14 2.407623081398995 1101 15 1.9712024507348573 1101 16 2.037594943666953 1101 17 2.400707441889942 1101 18 2.373516403604512 1101 19 1.8965024347202117 1101 20 1.589965414973264 1101 21 1.6317341276284338 1101 22 1.8971554880665593 1101 23 2.2136129824351585 1101 24 1.895476253349912 1101 25 2.051610999293878 1102 1 0.9674790098756603 1102 2 1.274813336467254 1102 3 1.1845051642750128 1102 4 1.6086129688100002 1102 5 1.5707527019599041 1102 6 1.2275037091208034 1102 7 1.2215360909865325 1102 8 1.277407304597297 1102 9 1.6763436266424137 1102 10 1.8646795744951916 1102 11 1.4876660207444203 1102 12 1.3667518938722125 1102 13 1.2634248016449163 1102 14 2.303912396349446 1102 15 1.8276411265929653 1102 16 2.002323508940542 1102 17 2.3110262699812765 1102 18 2.5973225934652007 1102 19 1.9857796859050438 1102 20 1.729285751035306 1102 21 1.5659453153348863 1102 22 1.7198852740783366 1102 23 2.0279832251466345 1102 24 1.838656635369162 1102 25 2.079035241718124 1103 1 1.0692091620642934 1103 2 1.3073464249138418 1103 3 1.1047463083320572 1103 4 1.638165398683661 1103 5 1.5087153689966328 1103 6 1.2290513351772034 1103 7 1.1450769682894446 1103 8 1.2258616760429675 1103 9 1.6419919352137242 1103 10 1.9019852911358583 1103 11 1.5677279187778907 1103 12 1.4621245576708901 1103 13 1.5215237964457984 1103 14 2.3652566486598943 1103 15 1.937598621851528 1103 16 2.0211517181554015 1103 17 2.4710430069460045 1103 18 2.638173175404141 1103 19 1.9911194119458813 1103 20 1.7281991274305852 1103 21 1.6366129961718983 1103 22 1.894604064335013 1103 23 2.346077019433359 1103 24 2.0759481417100005 1103 25 2.1171526625995227 1104 1 0.6343492357737333 1104 2 0.9263848394858769 1104 3 0.8112585361710565 1104 4 1.235356683640724 1104 5 1.1142913175792017 1104 6 0.8045360121798808 1104 7 0.738075307802049 1104 8 0.8229645980344245 1104 9 1.1564435755126397 1104 10 1.2503783737883651 1104 11 0.8455570370750394 1104 12 0.9459510668060247 1104 13 0.9303326023062297 1104 14 1.911985316674091 1104 15 1.4540470197205053 1104 16 1.576282231482871 1104 17 2.0313562536590837 1104 18 2.1095791651859486 1104 19 1.466626916048014 1104 20 1.152898539939585 1104 21 1.1391607950136091 1104 22 1.4657982284520372 1104 23 1.7711397019120838 1104 24 1.4339402651334296 1104 25 1.6914389168811326 1105 1 0.8470508823540861 1105 2 1.099654105808824 1105 3 1.0088403076647623 1105 4 1.2912283686469408 1105 5 1.2620051719667131 1105 6 1.0701134941934995 1105 7 0.9843801264008007 1105 8 0.9681370176779467 1105 9 1.2220585931006624 1105 10 1.6102504310019583 1105 11 1.158244988243112 1105 12 1.1766022740400004 1105 13 1.13272106121903 1105 14 1.9601097360839361 1105 15 1.5876860831641935 1105 16 1.8086118700866507 1105 17 2.0438215275682055 1105 18 1.5576947166851554 1105 19 1.5400846123537306 1105 20 1.3130119022717466 1105 21 1.3041605160329865 1105 22 1.470219590286753 1105 23 1.8128269277305407 1105 24 1.633385084953055 1105 25 1.9226100425443278 1106 1 0.4868196845341508 1106 2 0.6382180337780241 1106 3 0.608370989872423 1106 4 1.0702803601776694 1106 5 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1107 18 1.6442804984950854 1107 19 1.2934703815870918 1107 20 1.1731998674973525 1107 21 0.8531925758122654 1107 22 1.18468504292791 1107 23 1.4567060266515028 1107 24 1.2984651664485583 1107 25 1.4999491891625354 1108 1 0.5900434231262324 1108 2 0.7986662355902006 1108 3 0.623003540777038 1108 4 1.1355278503537747 1108 5 0.9448821699771983 1108 6 0.6494027702238583 1108 7 0.5742507100795251 1108 8 0.6112409446442157 1108 9 0.768234614427783 1108 10 1.1235553819968587 1108 11 0.7506146743080251 1108 12 0.5775062597837888 1108 13 0.47416353739598094 1108 14 1.4864730957379706 1108 15 1.0099215024507515 1108 16 1.0760864836726896 1108 17 1.405998523048531 1108 18 1.7190235252945953 1108 19 1.11023917116988 1108 20 0.8798564387759598 1108 21 0.7604933284696571 1108 22 1.0359385871966609 1108 23 1.3961356858095968 1108 24 1.011433618802549 1108 25 1.3229770254864093 1109 1 0.36001661548043984 1109 2 0.5006442657893224 1109 3 0.4364943118813045 1109 4 0.6212414116975609 1109 5 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 112417.0 3 3.0 4 891.0 5 5516.0 6 6533.0 7 5889.0 8 5407.0 9 5122.0 10 4744.0 11 4492.0 12 4382.0 13 4486.0 14 4516.0 15 4596.0 16 4792.0 17 4963.0 18 5622.0 19 7139.0 20 8862.0 21 11408.0 22 14102.0 23 16690.0 24 19553.0 25 22412.0 26 25225.0 27 28494.0 28 31299.0 29 37146.0 30 45091.0 31 53405.0 32 67873.0 33 87746.0 34 114664.0 35 151736.0 36 214042.0 37 306201.0 38 761267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55949029761362 18.551202214267477 12.162915373566932 25.72639211455197 2 14.53413403254569 20.322606560063107 41.33206489893752 23.81119450845368 3 18.33447560604754 25.06846828037273 29.6904970474898 26.90655906608993 4 11.426427047567266 16.792263187634703 39.40945617471653 32.37185359008151 5 12.643998994242308 38.02022771152909 36.51000265962999 12.82577063459861 6 31.466775226451755 37.60500691253526 18.429321647760027 12.498896213252959 7 27.820394618866573 31.54242976976038 22.76987630348665 17.8672993078864 8 24.97672476720239 36.515851610881576 21.0208861252443 17.486537496671733 9 26.615701669102158 14.877148302799467 20.049105357800276 38.458044670298094 10 13.7144665924609 28.75358764356235 35.98471023070507 21.547235533271685 11 34.443197626396554 22.31909968265009 24.77616144271037 18.461541248242984 12 22.91636474961415 24.743355409508215 32.01952618345446 20.320753657423175 13 28.80551126222922 21.766397223355657 25.84948460984913 23.578606904565984 14 20.673886196622824 21.310112368709575 28.76739500501998 29.248606429647623 15 23.2185101291812 30.15414690177545 23.48796034475496 23.139382624288384 16 22.364536124303978 27.88181123271493 27.53035868899733 22.223293953983763 17 21.83917417068534 27.97301587948639 28.12760296709374 22.060206982734528 18 21.691450538083405 27.414083736484507 29.3369812438463 21.55748448158578 19 23.385472638978896 27.052025634441563 27.409252111408648 22.153249615170896 20 23.61000281690396 27.18991638958054 27.670012834575274 21.530067958940226 21 23.38496043160417 26.702971853063634 26.912170525677904 22.999897189654288 22 23.209359060361646 27.450341819677384 27.205566952312836 22.134732167648135 23 22.610048779736687 27.240749806733334 27.548798984098923 22.60040242943106 24 23.032131115461656 26.95767701466089 27.700777571725848 22.3094142981516 25 22.91243755406697 27.035159947642796 27.5825554483652 22.469847049925043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 165.0 1 165.0 2 272.5 3 380.0 4 380.0 5 380.0 6 768.5 7 1157.0 8 1157.0 9 1157.0 10 1966.0 11 2775.0 12 2775.0 13 2775.0 14 5582.5 15 8390.0 16 8390.0 17 8390.0 18 16071.5 19 23753.0 20 23753.0 21 23753.0 22 38591.5 23 53430.0 24 53430.0 25 53430.0 26 77086.5 27 100743.0 28 100743.0 29 100743.0 30 130269.5 31 159796.0 32 159796.0 33 159796.0 34 185923.0 35 212050.0 36 212050.0 37 212050.0 38 233363.0 39 254676.0 40 254676.0 41 254676.0 42 268337.5 43 281999.0 44 281999.0 45 281999.0 46 287253.0 47 292507.0 48 292507.0 49 292507.0 50 281627.0 51 270747.0 52 270747.0 53 270747.0 54 241222.0 55 211697.0 56 211697.0 57 211697.0 58 181406.0 59 151115.0 60 151115.0 61 151115.0 62 122328.0 63 93541.0 64 93541.0 65 93541.0 66 72181.0 67 50821.0 68 50821.0 69 50821.0 70 37310.5 71 23800.0 72 23800.0 73 23800.0 74 16756.5 75 9713.0 76 9713.0 77 9713.0 78 6692.5 79 3672.0 80 3672.0 81 3672.0 82 2486.0 83 1300.0 84 1300.0 85 1300.0 86 840.0 87 380.0 88 380.0 89 380.0 90 231.5 91 83.0 92 83.0 93 83.0 94 54.5 95 26.0 96 26.0 97 26.0 98 18.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16991695665283968 2 0.24833320203592477 3 0.2497367260583703 4 0.4055278925498228 5 0.2450281293379079 6 0.01897021178724749 7 0.018924936818781508 8 0.01675173833241425 9 0.018698561976451582 10 0.020464285746624976 11 0.019830436188101194 12 0.027798830638114457 13 0.026848056300328785 14 0.02485595768782547 15 0.040611646713988064 16 0.02091703543128482 17 0.041154946335579874 18 0.031873577800053066 19 0.027300805984988633 20 0.028432680196638242 21 0.035178650498069924 22 0.044505294002062726 23 0.0288401549128321 24 0.04907806581712716 25 0.036944374268243325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2208726.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.92445576558316 #Duplication Level Percentage of deduplicated Percentage of total 1 85.67773611005458 65.90713221493199 2 10.083290896443005 15.513033290698743 3 2.2776036851116044 5.256102717806903 4 0.8161499911979897 2.511275755839633 5 0.35642085839991944 1.370874027795789 6 0.20999074938671128 0.9692054467427837 7 0.11769702894958475 0.6337645928021024 8 0.09050171359267163 0.5569436051175155 9 0.06578880368833703 0.4554691127274703 >10 0.28156476070259645 3.671654790759693 >50 0.013203224927965572 0.6920944473981626 >100 0.00868579598531271 1.3587686407237827 >500 7.723026207376452E-4 0.40375242375315673 >1k 5.940789390275405E-4 0.6999289329023395 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.5274968465984465E-5 0.0 5 0.0 0.0 0.0 4.5274968465984465E-5 0.0 6 0.0 0.0 0.0 4.5274968465984465E-5 0.0 7 0.0 0.0 0.0 4.5274968465984465E-5 0.0 8 0.0 0.0 0.0 4.5274968465984465E-5 0.0 9 0.0 0.0 0.0 4.5274968465984465E-5 0.0 10 0.0 0.0 0.0 4.5274968465984465E-5 0.0 11 0.0 0.0 0.0 4.5274968465984465E-5 0.0 12 0.0 0.0 0.0 9.054993693196893E-5 0.0 13 0.0 0.0 0.0 9.054993693196893E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 640 0.0 13.094439 4 ACCGTGC 110 3.8802682E-8 12.076555 8 GTGCGCC 115 7.171184E-8 11.554367 11 CTAGTAC 145 1.1677912E-9 11.165926 3 CGTGCGC 85 5.379851E-5 11.165226 10 GTCCTAC 1310 0.0 11.075025 1 AGGACCT 1060 0.0 11.033735 5 GGACCTG 1005 0.0 10.762839 6 TCCTACA 1410 0.0 10.746777 2 GACCGTG 115 8.9569585E-7 10.726381 7 GTCCTAT 285 0.0 10.382195 1 GTCTTAT 285 0.0 10.382195 1 AGAACCG 110 5.981703E-6 10.373179 5 GTCCTAA 700 0.0 10.226702 1 GGACGTG 1010 0.0 10.145897 6 GTAGGAC 1305 0.0 10.071227 3 TGGCGAG 1145 0.0 10.03079 18 CCTACAG 1475 0.0 9.943574 3 TGTAGGA 1420 0.0 9.932842 2 TAGACTG 250 0.0 9.889098 5 >>END_MODULE