FastQCFastQC Report
Thu 26 May 2016
SRR1513166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208726
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25010.11323269613342714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC852.7375245E-713.3968698
GTCGCGT500.001510444813.28763518
TAGGACC6650.012.453684
GTAGGAC12750.010.8259943
GTATTAA2250.010.5814461
GTCTTAC1855.638867E-1110.2954611
TGTAGGA14150.010.2942392
TATAGGT2350.010.12684
TGGCGAG12000.010.12391318
TAGGACA6400.010.1141414
AACCGAG856.6477613E-410.0501557
GGCGAGG4750.09.9873119
CGTTTCC1252.5985555E-69.87170914
AGGACCT11600.09.847445
CTATACT3300.09.80765154
CTGTAGG14650.09.750821
TAAGGTG2550.09.7058425
GTCCTAC15100.09.58636951
TAGGACT3200.09.5191934
TCCTACA16350.09.4913742
Produced by FastQC (version 0.11.5)