FastQCFastQC Report
Thu 26 May 2016
SRR1513165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537796
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT28150.11092302139336654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC400.005330732614.2296046
GTCCTAA8650.012.26151
ACGGCTC550.003101551212.0740788
GTATTCG657.646074E-411.7601641
GGACCGA658.1235106E-411.6755726
GGCGAGG6150.011.2633919
TAGGACC6300.011.19504454
GTCCTAC16550.011.0273751
GGACGTG11950.010.95500856
AGGACGT12300.010.7481545
TAGGACT4350.010.7359764
TGGCGAG13550.010.5044518
GTAGGAC14500.010.4513513
TAAAGTG9950.010.2278625
TTAGGAC9600.010.2260833
GTGTAGG3650.010.2095951
TCCTACA18750.010.1741682
AAGGCGT1409.900941E-810.1640026
GTATTAA3200.010.1523291
TAGGACG13400.010.0999274
Produced by FastQC (version 0.11.5)