##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513165_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2537796 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.543412866912863 31.0 30.0 34.0 25.0 34.0 2 29.412728997917878 31.0 30.0 34.0 23.0 34.0 3 29.62683131347043 31.0 30.0 34.0 25.0 34.0 4 32.69326494328149 37.0 33.0 37.0 24.0 37.0 5 32.871388007546706 37.0 35.0 37.0 25.0 37.0 6 33.23124711363719 37.0 35.0 37.0 27.0 37.0 7 33.18927092642592 37.0 35.0 37.0 27.0 37.0 8 33.07780333801456 37.0 35.0 37.0 26.0 37.0 9 34.33720480290772 39.0 35.0 39.0 25.0 39.0 10 33.91884020622619 39.0 34.0 39.0 22.0 39.0 11 34.39305956822376 39.0 35.0 39.0 27.0 39.0 12 34.38181358942957 39.0 35.0 39.0 27.0 39.0 13 34.435921957478065 39.0 35.0 39.0 27.0 39.0 14 34.65953804009463 40.0 34.0 41.0 17.0 41.0 15 35.10561250786115 40.0 35.0 41.0 24.0 41.0 16 34.96108237226318 40.0 35.0 41.0 22.0 41.0 17 34.572971980411346 39.0 34.0 41.0 17.0 41.0 18 34.30659004900315 39.0 33.0 41.0 17.0 41.0 19 34.93787207482398 40.0 34.0 41.0 22.0 41.0 20 35.151720626874656 40.0 35.0 41.0 25.0 41.0 21 35.20003065652243 40.0 36.0 41.0 25.0 41.0 22 34.8841364711742 40.0 35.0 41.0 19.0 41.0 23 34.43801984083827 40.0 34.0 41.0 16.0 41.0 24 34.69034587492454 40.0 35.0 41.0 18.0 41.0 25 34.34107509035399 39.0 34.0 41.0 15.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0083323908225381 1101 2 1.2451669027969459 1101 3 1.1392169321467982 1101 4 1.4501639600852272 1101 5 1.2682845908010307 1101 6 0.9386272877458026 1101 7 0.9412423093628988 1101 8 1.053631533317791 1101 9 1.4451311036326544 1101 10 1.7304363622540748 1101 11 1.2880259833778638 1101 12 1.208465224096102 1101 13 1.0386622477718461 1101 14 2.083073122232335 1101 15 1.5901914304563292 1101 16 1.6867607895766241 1101 17 2.114618135718409 1101 18 2.166485716478242 1101 19 1.713450751940421 1101 20 1.2856213733700912 1101 21 1.2741130233287166 1101 22 1.5233073211590735 1101 23 1.790373564495276 1101 24 1.4404542589579563 1101 25 1.7129523451645952 1102 1 0.7012253382471343 1102 2 0.9413286346780474 1102 3 0.8602930350322424 1102 4 1.0886844981027721 1102 5 1.0231961639957987 1102 6 0.817150740661873 1102 7 0.7525271263775224 1102 8 0.782514541739566 1102 9 1.1190111170424188 1102 10 1.3802966566526678 1102 11 0.9774803673729195 1102 12 0.9145309861787609 1102 13 0.8725845179662954 1102 14 1.8661726268844632 1102 15 1.476594661371756 1102 16 1.596053831165385 1102 17 1.956152728107135 1102 18 2.170977156043705 1102 19 1.5722795358546193 1102 20 1.3063669156706297 1102 21 1.1936405632652907 1102 22 1.3370668746927592 1102 23 1.7146925073916108 1102 24 1.3861101974012158 1102 25 1.708150311760967 1103 1 0.8835747783756496 1103 2 1.1327755718623287 1103 3 0.9627079236519158 1103 4 1.462532358024248 1103 5 1.2990149560675661 1103 6 0.9769546424255182 1103 7 0.9722766842150321 1103 8 0.9567313879948571 1103 9 1.3909393163661932 1103 10 1.6502889440603994 1103 11 1.1994092300158101 1103 12 1.2116019197159957 1103 13 1.2080539398595675 1103 14 2.086130451849023 1103 15 1.7171777158598047 1103 16 1.716013284276869 1103 17 2.1129398093040237 1103 18 2.2829448952169784 1103 19 1.7188814273995305 1103 20 1.3972108053256846 1103 21 1.484006245063874 1103 22 1.735668296487134 1103 23 2.081058278436771 1103 24 1.8733126610890167 1103 25 2.0106207382811903 1104 1 0.5429287941792253 1104 2 0.8237699149143651 1104 3 0.6840779501985743 1104 4 1.080859814388056 1104 5 1.058310109822962 1104 6 0.8142085782662534 1104 7 0.6604380834044079 1104 8 0.7446997548342011 1104 9 1.023582729162186 1104 10 1.2358360689038648 1104 11 0.8134747105904125 1104 12 0.7835610506736685 1104 13 0.6737532209180515 1104 14 1.6592238981395653 1104 15 1.206270135777089 1104 16 1.341108287711286 1104 17 1.7135672032298785 1104 18 1.9203304450311407 1104 19 1.2657545537899537 1104 20 1.0903631033208754 1104 21 1.0909126761286743 1104 22 1.3034945240770526 1104 23 1.5723960560874701 1104 24 1.370307829997678 1104 25 1.5888003176942362 1105 1 0.3987415753067154 1105 2 0.7022355154313686 1105 3 0.5691040455939884 1105 4 0.9622082598057418 1105 5 0.8259986593598256 1105 6 0.5075816139500446 1105 7 0.48717509586011687 1105 8 0.4461047389996864 1105 9 0.7761029952702714 1105 10 1.1467974178624374 1105 11 0.6091725584136682 1105 12 0.5687979254015545 1105 13 0.5628938029407493 1105 14 1.2583768155312214 1105 15 0.9064358246454489 1105 16 1.2009029664679716 1105 17 1.4876025307994212 1105 18 1.1507212866374346 1105 19 0.937287579274809 1105 20 0.8462771083855003 1105 21 0.7906287723965946 1105 22 0.9944835089854465 1105 23 1.2775110539003691 1105 24 1.049859537775312 1105 25 1.2908697046731206 1106 1 0.5589890881081878 1106 2 0.8200559132557004 1106 3 0.750704404665246 1106 4 1.2431498024104073 1106 5 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1107 18 1.6570127285640197 1107 19 1.1579493874539892 1107 20 0.8463548365497857 1107 21 0.6951413637306842 1107 22 1.062631903993136 1107 23 1.3615351994946536 1107 24 1.0090148190933022 1107 25 1.4450325192679188 1108 1 0.7544558494596494 1108 2 0.9320238129333518 1108 3 0.7809174930696905 1108 4 1.2928236772292223 1108 5 1.1035408078767404 1108 6 0.7859396283503699 1108 7 0.7017602047133664 1108 8 0.8031695668934873 1108 9 1.1420092638663277 1108 10 1.3346529066062232 1108 11 1.060311322501704 1108 12 0.8214933436108112 1108 13 0.8846644594786426 1108 14 1.6739436195912276 1108 15 1.2032912720944537 1108 16 1.2963749073565367 1108 17 1.6187213433524477 1108 18 1.913369888186594 1108 19 1.2902964573758666 1108 20 1.1593730250926129 1108 21 1.092833242705268 1108 22 1.35258022219071 1108 23 1.6354602264588038 1108 24 1.3315958387161757 1108 25 1.723456806051395 1109 1 0.5752951297033988 1109 2 0.6605449446181986 1109 3 0.6977941981475304 1109 4 1.0221304739508241 1109 5 1.0426831677427302 1109 6 0.6773916587172764 1109 7 0.6509619136238811 1109 8 0.6207008081281487 1109 9 0.8330870247829765 1109 10 1.2218831912953476 1109 11 0.7872823654347698 1109 12 0.6224988365374529 1109 13 0.6240097597240677 1109 14 1.5076159565033933 1109 15 0.9992059062568828 1109 16 1.1569778099501633 1109 17 1.515580543470243 1109 18 1.8190851362627996 1109 19 1.042591443658722 1109 20 0.9208177436952383 1109 21 0.8990660138164372 1109 22 1.078163652693199 1109 23 1.3989265647689422 1109 24 1.1489305647413914 1109 25 1.3889764413444965 1110 1 0.41579778349798246 1110 2 0.6041825355111534 1110 3 0.5250758697324827 1110 4 0.8690348137926023 1110 5 0.7819303900697179 1110 6 0.43949519897419265 1110 7 0.37440530475847567 1110 8 0.4114951777397593 1110 9 0.7220818732611249 1110 10 1.0239187642379548 1110 11 0.5922147427691158 1110 12 0.5399654999093642 1110 13 0.39405621576055694 1110 14 1.2075853282934474 1110 15 0.823536865199479 1110 16 1.115725772946604 1110 17 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2216 23 -2.1269310132741595 2216 24 -2.429877317920827 2216 25 -1.9484612290937307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 109733.0 3 8.0 4 889.0 5 5412.0 6 6507.0 7 6035.0 8 5433.0 9 5135.0 10 4900.0 11 4624.0 12 4541.0 13 4553.0 14 4408.0 15 4723.0 16 4816.0 17 4958.0 18 5870.0 19 7061.0 20 8977.0 21 11576.0 22 14370.0 23 16948.0 24 19767.0 25 22678.0 26 25946.0 27 28775.0 28 32690.0 29 38421.0 30 47111.0 31 56326.0 32 71522.0 33 93354.0 34 124422.0 35 167173.0 36 240288.0 37 352532.0 38 975314.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95223460180897 18.31642444763561 12.043663371729965 25.687677578825458 2 14.472456645103318 20.169298169703595 41.35310507084451 24.005140114348578 3 18.58270771912896 24.74196007212197 29.63402013582733 27.04131207292174 4 11.334548265434945 16.706702401827307 39.24903890475534 32.7097104279824 5 12.631247453879407 37.921796609861424 36.4753827923144 12.971573143944767 6 31.7829741499076 37.30167269476838 18.288026995806145 12.62732615951788 7 27.968631086171108 31.41313425471044 22.528493994968645 18.089740664149804 8 24.984530934583784 36.538878392257665 20.97258448080299 17.50400619235556 9 26.505794747010402 14.84898502265008 19.974240313908734 38.67097991643079 10 13.621587321606635 28.839443014626188 35.99575127205654 21.543218391710646 11 34.75926597405054 22.20192019674922 24.506195708722863 18.53261812047737 12 22.976731316351458 24.653437170139586 32.00652093881969 20.363310574689265 13 29.02450214498219 21.581666196310408 25.769150725793864 23.624680932913535 14 20.354856395333485 21.40481857714536 28.83641771302242 29.403907314498735 15 23.233712523933477 30.082086348547083 23.448675237580513 23.23552588993893 16 22.27458451851364 27.79870386566174 27.52967105556162 22.397040560262997 17 21.652746674004113 28.026075791984972 28.13243197323829 22.188745560772624 18 21.561738760258258 27.38870625704578 29.55778129902483 21.491773683671138 19 23.485681875244126 26.934818073713323 27.383322448197728 22.196177602844823 20 23.735398744592583 27.127639656684504 27.53208975079079 21.604871847932124 21 23.38342071031574 26.61413536205605 26.952895265875675 23.049548661752535 22 23.15983764247283 27.516967433880623 27.142099104952095 22.181095818694455 23 22.516578479352187 27.233325344971114 27.601079059559545 22.64901711611715 24 23.088907935001068 27.009415209888772 27.596673255710353 22.30500359939981 25 22.94129449565189 26.974526272553078 27.492215736924035 22.591963494871 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 196.0 1 196.0 2 337.5 3 479.0 4 479.0 5 479.0 6 969.0 7 1459.0 8 1459.0 9 1459.0 10 2387.0 11 3315.0 12 3315.0 13 3315.0 14 6427.0 15 9539.0 16 9539.0 17 9539.0 18 18140.5 19 26742.0 20 26742.0 21 26742.0 22 43438.5 23 60135.0 24 60135.0 25 60135.0 26 86913.0 27 113691.0 28 113691.0 29 113691.0 30 147734.0 31 181777.0 32 181777.0 33 181777.0 34 211242.5 35 240708.0 36 240708.0 37 240708.0 38 265035.0 39 289362.0 40 289362.0 41 289362.0 42 305844.0 43 322326.0 44 322326.0 45 322326.0 46 330602.0 47 338878.0 48 338878.0 49 338878.0 50 325792.5 51 312707.0 52 312707.0 53 312707.0 54 279324.0 55 245941.0 56 245941.0 57 245941.0 58 210614.0 59 175287.0 60 175287.0 61 175287.0 62 142732.0 63 110177.0 64 110177.0 65 110177.0 66 84677.5 67 59178.0 68 59178.0 69 59178.0 70 43526.5 71 27875.0 72 27875.0 73 27875.0 74 19652.5 75 11430.0 76 11430.0 77 11430.0 78 7923.0 79 4416.0 80 4416.0 81 4416.0 82 2968.0 83 1520.0 84 1520.0 85 1520.0 86 1002.5 87 485.0 88 485.0 89 485.0 90 313.5 91 142.0 92 142.0 93 142.0 94 79.5 95 17.0 96 17.0 97 17.0 98 15.5 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1739304498864369 2 0.2802825759044462 3 0.2791004477901297 4 0.4946024030300308 5 0.2752388292833624 6 0.020214390754812444 7 0.01997796513194914 8 0.018677624206200973 9 0.019623326697654184 10 0.022302817090104957 11 0.021908774385332782 12 0.02908035161218632 13 0.028055840579778675 14 0.025888605703531728 15 0.04243839930396297 16 0.022026987196764436 17 0.04086222848487428 18 0.03215388470940927 19 0.027464776522620415 20 0.030380692537934493 21 0.03530622634758665 22 0.04618180499929861 23 0.030420096808411706 24 0.04972818934224815 25 0.03731584414192472 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2537796.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.01252272368511 #Duplication Level Percentage of deduplicated Percentage of total 1 84.22601887950098 63.18006155124098 2 10.967441503549123 16.453909100113314 3 2.6430096080945495 5.947764548583314 4 0.9074374159552274 2.7227667913865434 5 0.41851914245615723 1.5697088341894847 6 0.24101818528191354 1.0847629260168534 7 0.12693070421701322 0.666497463408844 8 0.08605913770539815 0.516441041816554 9 0.06881796270230003 0.4645988091903596 >10 0.28919342225780426 3.6864508299260814 >50 0.014231396692858373 0.7459066527638933 >100 0.009466693590552185 1.4517551306751102 >500 0.0011665958833209124 0.5996773681363209 >1k 6.89352112830745E-4 0.9096989525523523 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2815 0.11092302139336654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.940427047721724E-5 2 0.0 0.0 0.0 0.0 3.940427047721724E-5 3 0.0 0.0 0.0 0.0 3.940427047721724E-5 4 0.0 0.0 0.0 1.1821281143165171E-4 3.940427047721724E-5 5 0.0 0.0 0.0 1.1821281143165171E-4 3.940427047721724E-5 6 0.0 0.0 0.0 1.1821281143165171E-4 3.940427047721724E-5 7 0.0 0.0 0.0 1.1821281143165171E-4 3.940427047721724E-5 8 3.940427047721724E-5 0.0 0.0 1.1821281143165171E-4 3.940427047721724E-5 9 3.940427047721724E-5 0.0 0.0 3.9404270477217236E-4 1.1821281143165171E-4 10 3.940427047721724E-5 0.0 0.0 3.9404270477217236E-4 1.1821281143165171E-4 11 3.940427047721724E-5 0.0 0.0 3.9404270477217236E-4 1.5761708190886896E-4 12 3.940427047721724E-5 0.0 0.0 3.9404270477217236E-4 1.9702135238608618E-4 13 3.940427047721724E-5 0.0 0.0 3.9404270477217236E-4 1.9702135238608618E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGC 40 0.0053307326 14.229604 6 GTCCTAA 865 0.0 12.2615 1 ACGGCTC 55 0.0031015512 12.074078 8 GTATTCG 65 7.646074E-4 11.760164 1 GGACCGA 65 8.1235106E-4 11.675572 6 GGCGAGG 615 0.0 11.26339 19 TAGGACC 630 0.0 11.1950445 4 GTCCTAC 1655 0.0 11.027375 1 GGACGTG 1195 0.0 10.9550085 6 AGGACGT 1230 0.0 10.748154 5 TAGGACT 435 0.0 10.735976 4 TGGCGAG 1355 0.0 10.50445 18 GTAGGAC 1450 0.0 10.451351 3 TAAAGTG 995 0.0 10.227862 5 TTAGGAC 960 0.0 10.226083 3 GTGTAGG 365 0.0 10.209595 1 TCCTACA 1875 0.0 10.174168 2 AAGGCGT 140 9.900941E-8 10.164002 6 GTATTAA 320 0.0 10.152329 1 TAGGACG 1340 0.0 10.099927 4 >>END_MODULE