FastQCFastQC Report
Thu 26 May 2016
SRR1513165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537796
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28840.11364191605629452No Hit
GTCCTACAGTGGACATTTCTAAATT25910.10209646480646987No Hit
GTACATGGGAAGCAGTGGTATCAAC25550.10067791106929005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7550.013.1135964
GGCGAGG7350.011.10301119
CTAGACA3100.011.0554454
TGGCGAG15600.011.01229518
TGTAGGA17800.010.912252
GTGCGTT700.001505444110.84544311
GCCTTAC1355.484253E-810.5823421
GTAGGAC17100.010.5785763
TATGGCG16600.010.52001816
TAGACAG4650.010.4412535
CGAGACG1002.4245164E-510.44038518
GTCCTAC14950.010.3841451
TCTAGAC2300.010.3486073
CTGTAGG17850.010.1910621
ATGGCGA17250.010.17923317
CCAACGA1409.858195E-810.16700319
GTCCTAA8150.010.1668391
GAGCCGT750.002605385610.1534266
TAGGACT3950.010.1224954
TCCAACG1701.5861588E-910.04956818
Produced by FastQC (version 0.11.5)