##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513165_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2537796 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.825573450348255 31.0 31.0 34.0 27.0 34.0 2 30.934558963762257 33.0 31.0 34.0 27.0 34.0 3 31.046515559170242 33.0 31.0 34.0 27.0 34.0 4 34.43441277391879 37.0 35.0 37.0 31.0 37.0 5 34.18093889343351 37.0 35.0 37.0 30.0 37.0 6 34.09527992005662 37.0 35.0 37.0 30.0 37.0 7 33.97168803166212 37.0 35.0 37.0 30.0 37.0 8 33.94595901325402 37.0 35.0 37.0 30.0 37.0 9 35.51226930769849 39.0 37.0 39.0 30.0 39.0 10 35.35943353996933 39.0 35.0 39.0 29.0 39.0 11 35.3763915618119 39.0 35.0 39.0 30.0 39.0 12 35.31192105275601 39.0 35.0 39.0 29.0 39.0 13 35.28383329471715 39.0 35.0 39.0 29.0 39.0 14 36.46595274009416 40.0 37.0 41.0 29.0 41.0 15 36.44781574247891 40.0 37.0 41.0 29.0 41.0 16 36.40748231930384 40.0 37.0 41.0 29.0 41.0 17 36.34406626852592 40.0 37.0 41.0 28.0 41.0 18 36.295292056571924 40.0 37.0 41.0 28.0 41.0 19 36.37345357940512 40.0 37.0 41.0 28.0 41.0 20 36.33718116034543 40.0 37.0 41.0 28.0 41.0 21 36.28584330655419 40.0 37.0 41.0 28.0 41.0 22 36.22932300310978 40.0 37.0 41.0 28.0 41.0 23 36.15231602540157 40.0 37.0 41.0 28.0 41.0 24 36.09278287143648 40.0 37.0 41.0 27.0 41.0 25 36.038833302598 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41700395375415766 1101 2 0.46254379239421795 1101 3 0.4155729893294762 1101 4 0.45106338433730997 1101 5 0.5354889840890991 1101 6 0.42019854308681204 1101 7 0.5309842875073585 1101 8 0.5255463298751337 1101 9 0.5905493567454698 1101 10 0.7585596312186595 1101 11 0.6459834013366432 1101 12 0.6845550839355923 1101 13 0.5697131087032048 1101 14 0.7722485837700432 1101 15 0.8148497952771692 1101 16 0.70617198918859 1101 17 0.6957040614272856 1101 18 0.6596452774975532 1101 19 0.7397714409723051 1101 20 0.7974329281991075 1101 21 0.7258675313633631 1101 22 0.750103540476438 1101 23 0.7194843663408079 1101 24 0.7039820715146163 1101 25 0.6445699007720549 1102 1 0.37552781340025376 1102 2 0.5287201542311095 1102 3 0.4184108556484105 1102 4 0.4446749497672329 1102 5 0.6263091798371647 1102 6 0.42342665454069817 1102 7 0.4486017369649886 1102 8 0.45405705707144506 1102 9 0.46796354595381473 1102 10 0.5655123112614078 1102 11 0.4911838021505943 1102 12 0.6154917092349379 1102 13 0.5060315117578682 1102 14 0.6772509317115549 1102 15 0.6183639118903628 1102 16 0.6680766381127938 1102 17 0.7664564441873551 1102 18 0.6577244025103397 1102 19 0.8130951586326489 1102 20 0.6852637198806093 1102 21 0.6772363319658155 1102 22 0.6813304231126409 1102 23 0.8239575954937379 1102 24 0.747128732199478 1102 25 0.7963911269028543 1103 1 0.4192060957896757 1103 2 0.4624181673491492 1103 3 0.47007075143494603 1103 4 0.5518273690428046 1103 5 0.5224800647108978 1103 6 0.4883822543711318 1103 7 0.5553611396642211 1103 8 0.5505740588449157 1103 9 0.6533212136514379 1103 10 0.6144295332568248 1103 11 0.6334503756309715 1103 12 0.6379336039879036 1103 13 0.7358188214983485 1103 14 0.7685126370326429 1103 15 0.7764825308358425 1103 16 0.7658838709444353 1103 17 0.8771751836157122 1103 18 0.6864467232589035 1103 19 0.7295893874548298 1103 20 0.7565078864412484 1103 21 0.7528882540535591 1103 22 0.720717106197462 1103 23 0.7353726843151875 1103 24 0.8340386118552985 1103 25 0.7794327706799891 1104 1 0.2529286781512887 1104 2 0.2669023368420014 1104 3 0.31382995209320796 1104 4 0.4608132846335806 1104 5 0.3855943657652645 1104 6 0.32228230207694963 1104 7 0.26163878042722644 1104 8 0.3970632114050474 1104 9 0.35754101002140715 1104 10 0.4655489649953566 1104 11 0.3886373765974298 1104 12 0.3869458896580511 1104 13 0.48448230871304787 1104 14 0.5163839579606915 1104 15 0.3996786846402287 1104 16 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-0.8293476542645877 2215 18 -0.9418539064732485 2215 19 -0.9059444691857976 2215 20 -0.934861268729172 2215 21 -0.8515477500842223 2215 22 -1.0225979349754226 2215 23 -0.9096339160129077 2215 24 -1.061351155214588 2215 25 -1.031071437262014 2216 1 -0.6004498752194998 2216 2 -0.4023728801865367 2216 3 -0.5090018356705137 2216 4 -0.4186710196083254 2216 5 -0.584141097719872 2216 6 -0.6005168612009442 2216 7 -0.7962692417630564 2216 8 -0.7271265683805552 2216 9 -0.9456265581761798 2216 10 -0.8934534828094272 2216 11 -0.8308578048205959 2216 12 -1.0120587906263907 2216 13 -0.9320121242608863 2216 14 -1.123340658279048 2216 15 -1.0938047811893696 2216 16 -1.1150186677268508 2216 17 -1.1627511231637513 2216 18 -1.1029030694333812 2216 19 -1.1429875892419048 2216 20 -1.2174602501358507 2216 21 -1.3241577401113602 2216 22 -1.1273547243645368 2216 23 -1.1757730862825255 2216 24 -1.2775469962051247 2216 25 -1.307970942170023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58809.0 3 2.0 4 1060.0 5 8269.0 6 9233.0 7 6948.0 8 5733.0 9 5077.0 10 4531.0 11 4114.0 12 3681.0 13 3598.0 14 3559.0 15 3486.0 16 3554.0 17 3812.0 18 4238.0 19 4991.0 20 6387.0 21 8217.0 22 10585.0 23 12780.0 24 14881.0 25 16400.0 26 18439.0 27 19866.0 28 23087.0 29 27409.0 30 35211.0 31 44763.0 32 58080.0 33 75309.0 34 100125.0 35 135228.0 36 195118.0 37 320110.0 38 1281106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.30705156674069 19.0660547742413 12.98088329632371 27.6460103626943 2 13.852742202502624 20.47646238912159 39.71012505236294 25.960670356012844 3 17.07456054450828 24.730880676410933 29.86906281414659 28.325495964934195 4 11.217176905529461 17.088283125135703 37.88436372147898 33.810176247855864 5 12.697156373620178 38.11426324691678 36.1793258854118 13.009254494051245 6 31.894006483751085 37.58705621886613 17.88637621570333 12.63256108167945 7 27.645604366367117 31.514421001888433 22.560561980174448 18.279412651570006 8 25.013586587053272 37.014395279631465 20.44092662297152 17.53109151034374 9 26.28831285515002 14.677222322927422 19.584459699092864 39.450005122829694 10 14.0543115769414 29.125847079096705 35.149984769619316 21.66985657434258 11 34.67359078507493 22.148943413891423 24.41220649729135 18.7652593037423 12 22.750016155750895 24.786665279636345 31.946697055240058 20.5166215093727 13 28.928802787930945 21.5593767190113 25.727481641550387 23.78433885150737 14 20.90602412297207 21.478736848078235 27.59896869152047 30.016270337429223 15 23.229841957351972 30.156876281623894 23.230866468384377 23.382415292639756 16 22.53443539197004 27.961112713551444 26.993225617819554 22.51122627665896 17 21.94811649254846 28.353567094595313 27.364666071011218 22.333650341845004 18 21.993777722069222 27.639748002867943 28.717708580917794 21.648765694145045 19 23.44431940155946 26.984202039880273 27.285920538924323 22.285558019635936 20 23.632947644333903 27.14922712463886 27.531527356808823 21.686297874218415 21 23.264243804600103 26.639135934304527 26.873554341015932 23.22306592007944 22 23.169993116060386 27.548052617414122 26.904185569260825 22.37776869726467 23 22.585067630086048 27.296711732373698 27.118474417842176 22.999746219698082 24 22.933443034822343 27.022187756620315 27.598356999538183 22.446012209019166 25 22.893808040141998 27.050533318149522 27.27537452847772 22.78028411323076 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 122.0 1 122.0 2 245.0 3 368.0 4 368.0 5 368.0 6 855.0 7 1342.0 8 1342.0 9 1342.0 10 2150.0 11 2958.0 12 2958.0 13 2958.0 14 5931.5 15 8905.0 16 8905.0 17 8905.0 18 17295.0 19 25685.0 20 25685.0 21 25685.0 22 41857.0 23 58029.0 24 58029.0 25 58029.0 26 84275.0 27 110521.0 28 110521.0 29 110521.0 30 144628.5 31 178736.0 32 178736.0 33 178736.0 34 207055.0 35 235374.0 36 235374.0 37 235374.0 38 259868.5 39 284363.0 40 284363.0 41 284363.0 42 301451.5 43 318540.0 44 318540.0 45 318540.0 46 330179.5 47 341819.0 48 341819.0 49 341819.0 50 330288.5 51 318758.0 52 318758.0 53 318758.0 54 284774.0 55 250790.0 56 250790.0 57 250790.0 58 214783.5 59 178777.0 60 178777.0 61 178777.0 62 146344.5 63 113912.0 64 113912.0 65 113912.0 66 87786.5 67 61661.0 68 61661.0 69 61661.0 70 45320.5 71 28980.0 72 28980.0 73 28980.0 74 20289.5 75 11599.0 76 11599.0 77 11599.0 78 8025.0 79 4451.0 80 4451.0 81 4451.0 82 2991.0 83 1531.0 84 1531.0 85 1531.0 86 988.5 87 446.0 88 446.0 89 446.0 90 278.5 91 111.0 92 111.0 93 111.0 94 63.0 95 15.0 96 15.0 97 15.0 98 9.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18405734739908172 2 0.008629535234510575 3 0.006580513169695279 4 0.002482469040064686 5 0.08097577583068143 6 0.3308382549267159 7 0.030223075456025626 8 0.014461367265138726 9 0.005358980784901544 10 0.00413744840010781 11 0.0 12 0.0 13 0.0 14 0.0019308092533836447 15 0.0 16 0.0 17 0.02108128470531122 18 0.030853543783661095 19 0.0 20 0.0 21 0.001024511032407648 22 1.9702135238608618E-4 23 0.006619917440172496 24 0.0 25 1.9702135238608618E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2537796.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.46099823941852 #Duplication Level Percentage of deduplicated Percentage of total 1 82.75685555399139 59.96644364597583 2 11.763948930154728 17.048549654331023 3 2.939353469198664 6.3896545966989935 4 1.0571580299218601 3.0641090457982023 5 0.487219058628773 1.7652189674755334 6 0.2705378590770073 1.1762065998165048 7 0.1633788002592871 0.828701367056256 8 0.11045595962706116 0.640299927685581 9 0.07480671334193938 0.4878512211390259 >10 0.345966752854021 4.220137343082108 >50 0.016939143624470944 0.8575172333429112 >100 0.010872964354500794 1.5600838906601895 >500 0.0016893416077841678 0.8423877270628981 >1k 8.174233585823174E-4 1.1528387798749176 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2884 0.11364191605629452 No Hit GTCCTACAGTGGACATTTCTAAATT 2591 0.10209646480646987 No Hit GTACATGGGAAGCAGTGGTATCAAC 2555 0.10067791106929005 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.940427047721724E-5 2 0.0 0.0 0.0 0.0 3.940427047721724E-5 3 0.0 0.0 0.0 0.0 3.940427047721724E-5 4 0.0 0.0 0.0 0.0 3.940427047721724E-5 5 0.0 0.0 0.0 0.0 3.940427047721724E-5 6 0.0 0.0 0.0 0.0 3.940427047721724E-5 7 0.0 0.0 0.0 0.0 3.940427047721724E-5 8 0.0 0.0 0.0 0.0 3.940427047721724E-5 9 0.0 0.0 0.0 2.7582989334052065E-4 1.1821281143165171E-4 10 0.0 0.0 0.0 2.7582989334052065E-4 1.1821281143165171E-4 11 0.0 0.0 0.0 2.7582989334052065E-4 1.9702135238608618E-4 12 0.0 0.0 0.0 2.7582989334052065E-4 1.9702135238608618E-4 13 0.0 0.0 0.0 2.7582989334052065E-4 1.9702135238608618E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 755 0.0 13.113596 4 GGCGAGG 735 0.0 11.103011 19 CTAGACA 310 0.0 11.055445 4 TGGCGAG 1560 0.0 11.012295 18 TGTAGGA 1780 0.0 10.91225 2 GTGCGTT 70 0.0015054441 10.845443 11 GCCTTAC 135 5.484253E-8 10.582342 1 GTAGGAC 1710 0.0 10.578576 3 TATGGCG 1660 0.0 10.520018 16 TAGACAG 465 0.0 10.441253 5 CGAGACG 100 2.4245164E-5 10.440385 18 GTCCTAC 1495 0.0 10.384145 1 TCTAGAC 230 0.0 10.348607 3 CTGTAGG 1785 0.0 10.191062 1 ATGGCGA 1725 0.0 10.179233 17 CCAACGA 140 9.858195E-8 10.167003 19 GTCCTAA 815 0.0 10.166839 1 GAGCCGT 75 0.0026053856 10.153426 6 TAGGACT 395 0.0 10.122495 4 TCCAACG 170 1.5861588E-9 10.049568 18 >>END_MODULE