FastQCFastQC Report
Thu 26 May 2016
SRR1513164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241470
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC32230.1437895666683025No Hit
GTCCTACAGTGGACATTTCTAAATT31260.1394620494586142No Hit
GTCCTAAAGTGTGTATTTCTCATTT30490.13602680383855237No Hit
CCCATGTACTCTGCGTTGATACCAC27600.12313347936845018No Hit
CTGTAGGACGTGGAATATGGCAAGA26890.11996591522527628No Hit
CTTTAGGACGTGAAATATGGCGAGG25730.11479074000544284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAA250.00584017819.1070841
TTGCGCC250.006075174518.9770379
TAGGACC7600.013.1679074
GGCGAGG7200.011.72886319
ACTGTCG658.118303E-411.6763538
TGTAGGA21100.011.3005742
TCGAACT1604.7293724E-1111.26761619
GTAGGAC19000.011.2366143
AGGACCT13650.011.217625
GGACCTG12900.011.1782756
ACACCGC600.00593804811.0679636
TGGCGAG17000.011.05133318
CTGTAGG20200.011.019681
GTATAAG3100.010.7862581
GGACGTG17400.010.740836
GTACTAG1701.2551027E-1010.6774891
AGGACGT17850.010.4962835
TTAGGAC12350.010.341393
GACGTGA9850.010.3057157
GTCCTAA10200.010.3028391
Produced by FastQC (version 0.11.5)