##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513164_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2241470 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.79264411301512 31.0 30.0 34.0 25.0 34.0 2 29.662070873132365 31.0 30.0 34.0 25.0 34.0 3 29.88085229782241 31.0 30.0 34.0 25.0 34.0 4 32.961658643657955 37.0 35.0 37.0 26.0 37.0 5 33.144404787929346 37.0 35.0 37.0 26.0 37.0 6 33.51058501786774 37.0 35.0 37.0 28.0 37.0 7 33.47379264500529 37.0 35.0 37.0 28.0 37.0 8 33.365530210085346 37.0 35.0 37.0 28.0 37.0 9 34.640676431092096 39.0 35.0 39.0 27.0 39.0 10 34.21906025956181 39.0 35.0 39.0 25.0 39.0 11 34.69716079180181 39.0 35.0 39.0 27.0 39.0 12 34.69904437712751 39.0 35.0 39.0 27.0 39.0 13 34.75113340798672 39.0 35.0 39.0 27.0 39.0 14 34.99486363859432 40.0 35.0 41.0 19.0 41.0 15 35.44736311438476 40.0 36.0 41.0 26.0 41.0 16 35.30883705782366 40.0 35.0 41.0 25.0 41.0 17 34.925261547109706 40.0 34.0 41.0 19.0 41.0 18 34.65807126573186 39.0 34.0 41.0 18.0 41.0 19 35.29144043864071 40.0 35.0 41.0 25.0 41.0 20 35.51021249447907 40.0 36.0 41.0 27.0 41.0 21 35.559347660240824 40.0 36.0 41.0 27.0 41.0 22 35.24873141286745 40.0 36.0 41.0 24.0 41.0 23 34.79917152582903 40.0 35.0 41.0 18.0 41.0 24 35.06242688949663 40.0 35.0 41.0 24.0 41.0 25 34.70696284134965 40.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7954326078363927 1101 2 1.1156207023533469 1101 3 0.9918906633820761 1101 4 1.4566133595689053 1101 5 1.208789748830938 1101 6 1.022162383043252 1101 7 0.9882849166335106 1101 8 1.140033751452819 1101 9 1.416891502047548 1101 10 1.75141232103568 1101 11 1.378660480174652 1101 12 1.2134657824020323 1101 13 1.185761377560567 1101 14 2.2242347601870875 1101 15 1.8493950572976274 1101 16 1.9300681983559542 1101 17 2.247636948513353 1101 18 2.340662136998226 1101 19 1.8820194168252868 1101 20 1.5194076186849657 1101 21 1.4086932036565258 1101 22 1.7301451750642514 1101 23 1.9694541614360546 1101 24 1.6603237326208387 1101 25 1.8953205126701675 1102 1 0.7192952342271575 1102 2 0.930531832382897 1102 3 0.8539339619871633 1102 4 1.160062468156255 1102 5 1.0855717908151021 1102 6 0.7705350935658899 1102 7 0.7097376643884559 1102 8 0.7886725789714006 1102 9 1.1598969753189223 1102 10 1.39368891000003 1102 11 0.9608516381131551 1102 12 0.8124469186446177 1102 13 0.8233827818574184 1102 14 1.7234015803325775 1102 15 1.3457575136516908 1102 16 1.4689991527547832 1102 17 1.7298624767925546 1102 18 1.9713462347834678 1102 19 1.418866657377933 1102 20 1.1972516831812285 1102 21 1.152564717808545 1102 22 1.241355943393252 1102 23 1.601502278384629 1102 24 1.381231312131149 1102 25 1.6808844537312453 1103 1 0.7178216069338639 1103 2 1.0094827692128838 1103 3 0.7484950728814788 1103 4 1.1971924107898815 1103 5 1.0843668359012426 1103 6 0.8316524917916013 1103 7 0.7432731430393886 1103 8 0.8147556978394164 1103 9 1.1552173406946409 1103 10 1.3416469484464812 1103 11 0.8764886045502536 1103 12 0.8526628971740351 1103 13 0.8846489746088366 1103 14 1.824663367268741 1103 15 1.321224103806152 1103 16 1.4449095997868753 1103 17 1.769939954757163 1103 18 2.0145249088408193 1103 19 1.372732961472579 1103 20 1.0587745137359903 1103 21 1.096359223397556 1103 22 1.38641975050988 1103 23 1.7997480564335433 1103 24 1.4792068924227806 1103 25 1.7350368644318337 1104 1 0.8689332213999137 1104 2 1.1058621026651032 1104 3 0.9220469336394714 1104 4 1.4389255443073026 1104 5 1.1207365994561087 1104 6 0.9272247343633993 1104 7 0.820353183489928 1104 8 0.988742856011882 1104 9 1.3013761086885012 1104 10 1.4092006582031615 1104 11 1.1244801075742643 1104 12 1.0827356719605987 1104 13 0.9530926626671103 1104 14 2.0233530075964055 1104 15 1.6098531495522508 1104 16 1.658511314836538 1104 17 2.0114975293936865 1104 18 2.21538512889677 1104 19 1.6231140774658641 1104 20 1.3845254017759387 1104 21 1.3549509938027242 1104 22 1.6441038205813143 1104 23 2.022503662595348 1104 24 1.8028366747408455 1104 25 1.9405338880534586 1105 1 0.5642077220206012 1105 2 0.8281318372720037 1105 3 0.7396692750871345 1105 4 1.1576529170668621 1105 5 0.9877114410699903 1105 6 0.7383974674746625 1105 7 0.6822876531749955 1105 8 0.6866138952629939 1105 9 0.9211263149137636 1105 10 1.4214841216769898 1105 11 0.807210486217727 1105 12 0.7272825937791225 1105 13 0.6703019325383437 1105 14 1.4362148881808352 1105 15 1.0929735478428313 1105 16 1.227287714044806 1105 17 1.5297907513958222 1105 18 1.2313362602679305 1105 19 1.0982026502116895 1105 20 0.9827123730154597 1105 21 0.8846766575777281 1105 22 1.1135269524434648 1105 23 1.3902173715156039 1105 24 1.199779207121317 1105 25 1.4224593123222107 1106 1 0.41088474776041295 1106 2 0.5634048360995116 1106 3 0.616913903535206 1106 4 1.0345853542694172 1106 5 0.8369322933166146 1106 6 0.5118172210209622 1106 7 0.44710848910253276 1106 8 0.36956594729959846 1106 9 0.6113565027429217 1106 10 0.5228547476736551 1106 11 0.31702393421038977 1106 12 0.44928764205346994 1106 13 0.3319485285007886 1106 14 1.3127292374483375 1106 15 0.9308155613344553 1106 16 1.1306051007912288 1106 17 1.3010744782497667 1106 18 1.5322100409926023 1106 19 1.0486057494193304 1106 20 0.8056241949529266 1106 21 0.696396600744734 1106 22 0.9062722309943325 1106 23 1.3787551635075417 1106 24 0.9298072156427182 1106 25 1.1993802197344436 1107 1 0.6726419605844995 1107 2 0.8874500419964058 1107 3 0.8257875235636405 1107 4 1.1285446991720747 1107 5 0.9854057731853416 1107 6 0.7132898639711271 1107 7 0.6935052166016717 1107 8 0.7858986808266621 1107 9 0.9990747887547897 1107 10 1.2217610452882184 1107 11 0.7158413586264558 1107 12 0.6431276404375907 1107 13 0.6835564571844372 1107 14 1.5831832020975298 1107 15 1.030466040257373 1107 16 1.1701330365706823 1107 17 1.5553262089499214 1107 18 1.6753660582438386 1107 19 1.2299270971655716 1107 20 1.0091439073269441 1107 21 0.9336082129238719 1107 22 1.1956264161579497 1107 23 1.466666476486104 1107 24 1.194142453778099 1107 25 1.441122520830163 1108 1 0.8200509337071828 1108 2 1.0704297274740604 1108 3 0.8444478147671504 1108 4 1.3793355911802507 1108 5 1.2842510834718155 1108 6 0.8822508346808178 1108 7 0.7903863003654337 1108 8 0.8444383086216121 1108 9 1.1304018921863985 1108 10 1.4151008003812535 1108 11 0.9914969746624323 1108 12 0.7547221481708988 1108 13 0.7329859349759786 1108 14 1.8390505273470126 1108 15 1.3497790343826708 1108 16 1.3855672942191148 1108 17 1.7298872337534732 1108 18 2.0110580205462725 1108 19 1.3897170013691493 1108 20 1.1942173800262097 1108 21 1.216480258941246 1108 22 1.4549595423171624 1108 23 1.7855677620766812 1108 24 1.4755827009111329 1108 25 1.7550620702516824 1109 1 0.3598350273880513 1109 2 0.5065738472480312 1109 3 0.4928157271345377 1109 4 0.7735534419192547 1109 5 0.7951686950240742 1109 6 0.44768732541017187 1109 7 0.41721568607899684 1109 8 0.4247261471782693 1109 9 0.6585980460594669 1109 10 0.9560337391423133 1109 11 0.5535272316298858 1109 12 0.4642363408256287 1109 13 0.31606671659196195 1109 14 1.3288999085801976 1109 15 0.8687753218408076 1109 16 0.9595115282705464 1109 17 1.3135010403655585 1109 18 1.4318322692841292 1109 19 0.9163774834169942 1109 20 0.7358609063651258 1109 21 0.593043111078579 1109 22 0.8392864153009825 1109 23 1.2150397106877406 1109 24 0.9076772808934166 1109 25 1.2078754886542953 1110 1 0.4579174903534948 1110 2 0.6981928256069665 1110 3 0.5952827351661085 1110 4 0.8980057292060195 1110 5 0.7283863593491802 1110 6 0.4412680216035767 1110 7 0.48902667153495827 1110 8 0.4700135996053021 1110 9 0.6328737481331856 1110 10 1.099581531804148 1110 11 0.6344788674944581 1110 12 0.5581232585504097 1110 13 0.34652955793004026 1110 14 1.271353643137303 1110 15 0.9116437437583329 1110 16 1.1363974684648142 1110 17 1.4109346318063132 1110 18 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25 -1.7561997968312326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83553.0 3 1.0 4 697.0 5 4301.0 6 5246.0 7 4699.0 8 4304.0 9 4034.0 10 3831.0 11 3718.0 12 3699.0 13 3717.0 14 3663.0 15 3902.0 16 3836.0 17 4166.0 18 4789.0 19 5846.0 20 7648.0 21 9709.0 22 12104.0 23 14582.0 24 16776.0 25 19579.0 26 22504.0 27 25103.0 28 28191.0 29 33606.0 30 40994.0 31 48936.0 32 63059.0 33 82837.0 34 110182.0 35 148450.0 36 214228.0 37 316681.0 38 878299.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64646217266092 18.4700668755821 12.163454576982 25.72001637477498 2 14.318650806609263 20.204753454420526 41.6204832185103 23.856112520459913 3 18.51303599779367 24.911167507013328 29.647439123516424 26.928357371676583 4 11.39817329408674 16.689156051826302 39.23324794838529 32.67942270570167 5 12.580016300735144 38.07290776664719 36.43831621100058 12.908759721617082 6 31.83221360311573 37.23186269085094 18.24654531694553 12.689378389087807 7 27.981192459134608 31.465281818603703 22.582257333280975 17.971268388980715 8 24.952800994893895 36.75681368005879 20.92262397464038 17.367761350406933 9 26.6297637454457 14.878570647675271 20.07974065323079 38.41192495364824 10 13.912156436457396 28.638695631026152 35.85372525447788 21.595422678038574 11 34.3319480707247 22.196985146171013 24.879884657141925 18.591182125962362 12 23.346847882113266 24.615415511012834 31.907247204675127 20.130489402198773 13 29.034110788212864 21.77755781822336 25.622407194521994 23.565924199041778 14 20.315743016226264 21.740812620795555 28.653074047480604 29.290370315497576 15 23.166847577696526 30.544268452925046 23.29382748228976 22.995056487088668 16 22.072290769539705 27.694683058524287 28.054307325018407 22.178718846917604 17 21.544594793030296 28.292517240594385 28.278011530477386 21.88487643589793 18 21.539091146028582 27.115488291221716 29.936957538496696 21.408463024253006 19 23.530955312480614 26.943356180552986 27.39983943428029 22.12584907268611 20 23.797672058543952 27.059842608236963 27.665168080015068 21.47731725320402 21 23.154946348229736 26.68289110362024 26.97066435543567 23.191498192714356 22 23.277234183673016 27.741762994557313 26.953747436885113 22.027255384884562 23 22.13244649493098 27.551568233941115 27.79420814907739 22.52177712205051 24 23.177567006480704 26.982118363125647 27.790298182019452 22.050016448374198 25 22.875746400385253 26.963871521168336 27.68761043422996 22.47277164421645 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 258.0 1 258.0 2 367.0 3 476.0 4 476.0 5 476.0 6 970.5 7 1465.0 8 1465.0 9 1465.0 10 2322.0 11 3179.0 12 3179.0 13 3179.0 14 6049.0 15 8919.0 16 8919.0 17 8919.0 18 16678.5 19 24438.0 20 24438.0 21 24438.0 22 39790.0 23 55142.0 24 55142.0 25 55142.0 26 78829.5 27 102517.0 28 102517.0 29 102517.0 30 133010.0 31 163503.0 32 163503.0 33 163503.0 34 188850.0 35 214197.0 36 214197.0 37 214197.0 38 234256.5 39 254316.0 40 254316.0 41 254316.0 42 267997.5 43 281679.0 44 281679.0 45 281679.0 46 291852.5 47 302026.0 48 302026.0 49 302026.0 50 289270.5 51 276515.0 52 276515.0 53 276515.0 54 245373.0 55 214231.0 56 214231.0 57 214231.0 58 182905.0 59 151579.0 60 151579.0 61 151579.0 62 123535.0 63 95491.0 64 95491.0 65 95491.0 66 73589.5 67 51688.0 68 51688.0 69 51688.0 70 37995.5 71 24303.0 72 24303.0 73 24303.0 74 17089.0 75 9875.0 76 9875.0 77 9875.0 78 6827.5 79 3780.0 80 3780.0 81 3780.0 82 2538.5 83 1297.0 84 1297.0 85 1297.0 86 872.5 87 448.0 88 448.0 89 448.0 90 282.5 91 117.0 92 117.0 93 117.0 94 71.5 95 26.0 96 26.0 97 26.0 98 15.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17318991554649404 2 0.26772609046741647 3 0.2713844039848849 4 0.48129129544450744 5 0.26714611393415927 6 0.020254565084520428 7 0.020254565084520428 8 0.01904999843852472 9 0.02029917866400175 10 0.022708311955993165 11 0.02226217616117994 12 0.030114166149892704 13 0.02917728098078493 14 0.026634306950349546 15 0.04273980914310698 16 0.023199061330287714 17 0.04340901283532682 18 0.033817093246842476 19 0.028686531606490383 20 0.030872597001075187 21 0.0364492944362405 22 0.046041214024724844 23 0.03127411921640709 24 0.05027950407545048 25 0.03939379068200779 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2241470.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.75842533264775 #Duplication Level Percentage of deduplicated Percentage of total 1 83.41167885415726 60.68902407780994 2 11.363968389914659 16.5364889116035 3 2.8436306081904648 6.20694255838973 4 0.984973707566063 2.8666054382626642 5 0.4591357113453453 1.6702995685736206 6 0.2462134726808487 1.0748462740764864 7 0.14167207007471477 0.7215485712576942 8 0.10317531322261825 0.6005498658624331 9 0.07742024921163816 0.506967787934996 >10 0.335619088208639 4.217249342157697 >50 0.018118998386936962 0.9143856653847544 >100 0.011542539646070436 1.671198909606439 >500 0.00179736792276764 0.8516070915164239 >1k 0.001053629471965882 1.472285937563735 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3223 0.1437895666683025 No Hit GTCCTACAGTGGACATTTCTAAATT 3126 0.1394620494586142 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3049 0.13602680383855237 No Hit CCCATGTACTCTGCGTTGATACCAC 2760 0.12313347936845018 No Hit CTGTAGGACGTGGAATATGGCAAGA 2689 0.11996591522527628 No Hit CTTTAGGACGTGAAATATGGCGAGG 2573 0.11479074000544284 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.461357948132253E-5 0.0 5 0.0 0.0 0.0 4.461357948132253E-5 4.461357948132253E-5 6 0.0 0.0 0.0 4.461357948132253E-5 4.461357948132253E-5 7 0.0 0.0 0.0 4.461357948132253E-5 4.461357948132253E-5 8 0.0 0.0 0.0 4.461357948132253E-5 4.461357948132253E-5 9 0.0 0.0 0.0 4.461357948132253E-5 4.461357948132253E-5 10 0.0 0.0 0.0 4.461357948132253E-5 4.461357948132253E-5 11 0.0 0.0 0.0 4.461357948132253E-5 4.461357948132253E-5 12 0.0 0.0 0.0 4.461357948132253E-5 8.922715896264505E-5 13 0.0 0.0 0.0 4.461357948132253E-5 8.922715896264505E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCTAA 25 0.005840178 19.107084 1 TTGCGCC 25 0.0060751745 18.977037 9 TAGGACC 760 0.0 13.167907 4 GGCGAGG 720 0.0 11.728863 19 ACTGTCG 65 8.118303E-4 11.676353 8 TGTAGGA 2110 0.0 11.300574 2 TCGAACT 160 4.7293724E-11 11.267616 19 GTAGGAC 1900 0.0 11.236614 3 AGGACCT 1365 0.0 11.21762 5 GGACCTG 1290 0.0 11.178275 6 ACACCGC 60 0.005938048 11.067963 6 TGGCGAG 1700 0.0 11.051333 18 CTGTAGG 2020 0.0 11.01968 1 GTATAAG 310 0.0 10.786258 1 GGACGTG 1740 0.0 10.74083 6 GTACTAG 170 1.2551027E-10 10.677489 1 AGGACGT 1785 0.0 10.496283 5 TTAGGAC 1235 0.0 10.34139 3 GACGTGA 985 0.0 10.305715 7 GTCCTAA 1020 0.0 10.302839 1 >>END_MODULE