FastQCFastQC Report
Thu 26 May 2016
SRR1513164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241470
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC35560.1586458886355829No Hit
CCCATGTACTCTGCGTTGATACCAC34970.15601368744618488No Hit
GTATCAACGCAGAGTACTTTTTTTT32980.1471355851294017No Hit
GTCCTACAGTGGACATTTCTAAATT32820.14642176785770053No Hit
GTCCTAAAGTGTGTATTTCTCATTT32240.14383418024778383No Hit
CTTTAGGACGTGAAATATGGCGAGG30380.13553605446425784No Hit
CTGTAGGACGTGGAATATGGCAAGA28780.12839788174724623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC500.00147480413.329823
TCCAACG701.1017895E-412.20179318
TAGGACC7950.011.6166644
CTAGTAC1408.069037E-910.8814873
TACACGG909.308969E-510.5787495
GGCGAGG9450.010.54264119
TGGCGAG19150.010.45665618
GTCCTAC17800.010.2743061
AAGTGCG750.002667470110.124557
GCAACTA750.002670434310.12319513
GTATTAC1701.4897523E-910.0854411
GTAGGAC19300.010.0146323
TGTAGGA19550.09.9848662
TTAGGAC12850.09.9288273
CTGTAGG19300.09.9199631
GGACGTG18600.09.82755856
GTCCTAA11350.09.8188651
GTACTAG1659.79162E-99.8137791
TCTAGTA2054.0017767E-119.75442
TAAGACT2450.09.7151784
Produced by FastQC (version 0.11.5)